Diversity analysis of protistan microplankton in Sagami Bay by 18S rRNA gene clone analysis using newly designed PCR primers

The diversity of protistan microplankton in Sagami Bay was revealed by 18S rRNA gene clone analysis using newly designed PCR primers. PCR amplification consisted of a first reaction targeting the V3–V5 region of the 18S rRNA gene, followed by a nested reaction targeting the V3–V4 region. In total, 6...

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