miRNA-218 contributes to the regulation of D-glucuronyl C5-epimerase expression in normal and tumor breast tissues
microRNAs (miRNAs) are key posttranscriptional regulators of gene expression. In the present study, regulation of tumor-suppressor gene D-glucuronyl C5-epimerase (GLCE) by miRNA-218 was investigated. Significant downregulation of miRNA-218 expression was shown in primary breast tumors. Exogenous miR...
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| Veröffentlicht in: | Epigenetics 2012-10, Vol.7 (10), p.1109-1114 |
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| creator | Prudnikova, Tatiana Y Mostovich, Luydmila A Kashuba, Vladimir I Ernberg, Ingemar Zabarovsky, Eugene R Grigorieva, Elvira V |
| description | microRNAs (miRNAs) are key posttranscriptional regulators of gene expression. In the present study, regulation of tumor-suppressor gene D-glucuronyl C5-epimerase (GLCE) by miRNA-218 was investigated. Significant downregulation of miRNA-218 expression was shown in primary breast tumors. Exogenous miRNA-218/anti-miRNA-218 did not affect GLCE mRNA but regulated GLCE protein level in MCF7 breast carcinoma cells in vitro. Comparative analysis showed a positive correlation between miRNA-218 and GLCE mRNA, and negative correlation between miRNA-218 and GLCE protein levels in breast tissues and primary tumors in vivo, supporting a direct involvement of miRNA-218 in posttranscriptional regulation of GLCE in human breast tissue. A common scheme for the regulation of GLCE expression in normal and tumor breast tissues is suggested. |
| doi_str_mv | 10.4161/epi.22103 |
| format | Article |
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In the present study, regulation of tumor-suppressor gene D-glucuronyl C5-epimerase (GLCE) by miRNA-218 was investigated. Significant downregulation of miRNA-218 expression was shown in primary breast tumors. Exogenous miRNA-218/anti-miRNA-218 did not affect GLCE mRNA but regulated GLCE protein level in MCF7 breast carcinoma cells in vitro. Comparative analysis showed a positive correlation between miRNA-218 and GLCE mRNA, and negative correlation between miRNA-218 and GLCE protein levels in breast tissues and primary tumors in vivo, supporting a direct involvement of miRNA-218 in posttranscriptional regulation of GLCE in human breast tissue. A common scheme for the regulation of GLCE expression in normal and tumor breast tissues is suggested.</description><identifier>ISSN: 1559-2294</identifier><identifier>ISSN: 1559-2308</identifier><identifier>EISSN: 1559-2308</identifier><identifier>DOI: 10.4161/epi.22103</identifier><identifier>PMID: 22968430</identifier><language>eng</language><publisher>United States: Landes Bioscience</publisher><subject>Binding ; Biology ; Bioscience ; biosynthesis ; breast cancer ; Breast Neoplasms - genetics ; Breast Neoplasms - metabolism ; Brief Report ; Calcium ; Cancer ; Carbohydrate Epimerases - genetics ; Carbohydrate Epimerases - metabolism ; Cell ; Cycle ; D-glucuronyl C5-epimerase ; expression ; Female ; Gene Expression Regulation, Neoplastic ; GLCE ; heparan sulphate proteoglycan ; Humans ; Landes ; MCF-7 Cells ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Organogenesis ; Proteins ; RNA, Messenger - genetics ; TECHNOLOGY ; TEKNIKVETENSKAP ; tumor-suppressor gene</subject><ispartof>Epigenetics, 2012-10, Vol.7 (10), p.1109-1114</ispartof><rights>Copyright © 2012 Landes Bioscience 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-39a80b2d3245c367dcaf9a287cd11c4412046858978b1fac67e74f886c0a78d63</citedby><cites>FETCH-LOGICAL-c540t-39a80b2d3245c367dcaf9a287cd11c4412046858978b1fac67e74f886c0a78d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469452/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469452/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,550,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22968430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-85632$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:125463499$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Prudnikova, Tatiana Y</creatorcontrib><creatorcontrib>Mostovich, Luydmila A</creatorcontrib><creatorcontrib>Kashuba, Vladimir I</creatorcontrib><creatorcontrib>Ernberg, Ingemar</creatorcontrib><creatorcontrib>Zabarovsky, Eugene R</creatorcontrib><creatorcontrib>Grigorieva, Elvira V</creatorcontrib><title>miRNA-218 contributes to the regulation of D-glucuronyl C5-epimerase expression in normal and tumor breast tissues</title><title>Epigenetics</title><addtitle>Epigenetics</addtitle><description>microRNAs (miRNAs) are key posttranscriptional regulators of gene expression. In the present study, regulation of tumor-suppressor gene D-glucuronyl C5-epimerase (GLCE) by miRNA-218 was investigated. Significant downregulation of miRNA-218 expression was shown in primary breast tumors. Exogenous miRNA-218/anti-miRNA-218 did not affect GLCE mRNA but regulated GLCE protein level in MCF7 breast carcinoma cells in vitro. Comparative analysis showed a positive correlation between miRNA-218 and GLCE mRNA, and negative correlation between miRNA-218 and GLCE protein levels in breast tissues and primary tumors in vivo, supporting a direct involvement of miRNA-218 in posttranscriptional regulation of GLCE in human breast tissue. A common scheme for the regulation of GLCE expression in normal and tumor breast tissues is suggested.</description><subject>Binding</subject><subject>Biology</subject><subject>Bioscience</subject><subject>biosynthesis</subject><subject>breast cancer</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - metabolism</subject><subject>Brief Report</subject><subject>Calcium</subject><subject>Cancer</subject><subject>Carbohydrate Epimerases - genetics</subject><subject>Carbohydrate Epimerases - metabolism</subject><subject>Cell</subject><subject>Cycle</subject><subject>D-glucuronyl C5-epimerase</subject><subject>expression</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>GLCE</subject><subject>heparan sulphate proteoglycan</subject><subject>Humans</subject><subject>Landes</subject><subject>MCF-7 Cells</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Organogenesis</subject><subject>Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>TECHNOLOGY</subject><subject>TEKNIKVETENSKAP</subject><subject>tumor-suppressor gene</subject><issn>1559-2294</issn><issn>1559-2308</issn><issn>1559-2308</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNqFkk1v1DAQhi0Eou3CgT-AfASJtP5K4lyQVtsClSpACLhajjPZGpw4tR1o_z0Ouy30gDjNaOaZeUczg9AzSo4FregJTPaYMUr4A3RIy7IpGCfy4a3PGnGAjmL8RojgVdM8Rgc5VknBySEKg_30fl0wKrHxYwq2nRNEnDxOl4ADbGenk_Uj9j0-LbZuNnPw443Dm7LIsgMEHQHD9RQgxoWzIx59GLTDeuxwmgcfcBtAx4STjXGG-AQ96rWL8HRvV-jLm7PPm3fFxYe355v1RWFKQVLBGy1JyzrORGl4VXdG941msjYdpUYIyoioZCmbWra016aqoRa9lJUhupZdxVeo2PWNP2GaWzUFO-hwo7y2ah_6nj1QJRfLNlbo1T_5U_t1rXzYKmdnJcuKs4y_3uGZHaAzkLen3b2q-5nRXqqt_6GyWiPKpcGLfYPgr_JekhpsNOCcHsHPUVFKWSUJF8toL3eoCT7GAP2dDCVqeQGVb6F-v0Bmn_891x15e_MMnOyALNRBbK2PxsJo4A9KKDv7eE4YY2rq-lzR_Kciy29hhGRNVDpk42A_zi9WodSp</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Prudnikova, Tatiana Y</creator><creator>Mostovich, Luydmila A</creator><creator>Kashuba, Vladimir I</creator><creator>Ernberg, Ingemar</creator><creator>Zabarovsky, Eugene R</creator><creator>Grigorieva, Elvira V</creator><general>Landes Bioscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>ABXSW</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>DG8</scope><scope>ZZAVC</scope></search><sort><creationdate>20121001</creationdate><title>miRNA-218 contributes to the regulation of D-glucuronyl C5-epimerase expression in normal and tumor breast tissues</title><author>Prudnikova, Tatiana Y ; Mostovich, Luydmila A ; Kashuba, Vladimir I ; Ernberg, Ingemar ; Zabarovsky, Eugene R ; Grigorieva, Elvira V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-39a80b2d3245c367dcaf9a287cd11c4412046858978b1fac67e74f886c0a78d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Binding</topic><topic>Biology</topic><topic>Bioscience</topic><topic>biosynthesis</topic><topic>breast cancer</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - metabolism</topic><topic>Brief Report</topic><topic>Calcium</topic><topic>Cancer</topic><topic>Carbohydrate Epimerases - genetics</topic><topic>Carbohydrate Epimerases - metabolism</topic><topic>Cell</topic><topic>Cycle</topic><topic>D-glucuronyl C5-epimerase</topic><topic>expression</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>GLCE</topic><topic>heparan sulphate proteoglycan</topic><topic>Humans</topic><topic>Landes</topic><topic>MCF-7 Cells</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Organogenesis</topic><topic>Proteins</topic><topic>RNA, Messenger - genetics</topic><topic>TECHNOLOGY</topic><topic>TEKNIKVETENSKAP</topic><topic>tumor-suppressor gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prudnikova, Tatiana Y</creatorcontrib><creatorcontrib>Mostovich, Luydmila A</creatorcontrib><creatorcontrib>Kashuba, Vladimir I</creatorcontrib><creatorcontrib>Ernberg, Ingemar</creatorcontrib><creatorcontrib>Zabarovsky, Eugene R</creatorcontrib><creatorcontrib>Grigorieva, Elvira V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SWEPUB Linköpings universitet full text</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Linköpings universitet</collection><collection>SwePub Articles full text</collection><jtitle>Epigenetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prudnikova, Tatiana Y</au><au>Mostovich, Luydmila A</au><au>Kashuba, Vladimir I</au><au>Ernberg, Ingemar</au><au>Zabarovsky, Eugene R</au><au>Grigorieva, Elvira V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miRNA-218 contributes to the regulation of D-glucuronyl C5-epimerase expression in normal and tumor breast tissues</atitle><jtitle>Epigenetics</jtitle><addtitle>Epigenetics</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>7</volume><issue>10</issue><spage>1109</spage><epage>1114</epage><pages>1109-1114</pages><issn>1559-2294</issn><issn>1559-2308</issn><eissn>1559-2308</eissn><abstract>microRNAs (miRNAs) are key posttranscriptional regulators of gene expression. In the present study, regulation of tumor-suppressor gene D-glucuronyl C5-epimerase (GLCE) by miRNA-218 was investigated. Significant downregulation of miRNA-218 expression was shown in primary breast tumors. Exogenous miRNA-218/anti-miRNA-218 did not affect GLCE mRNA but regulated GLCE protein level in MCF7 breast carcinoma cells in vitro. Comparative analysis showed a positive correlation between miRNA-218 and GLCE mRNA, and negative correlation between miRNA-218 and GLCE protein levels in breast tissues and primary tumors in vivo, supporting a direct involvement of miRNA-218 in posttranscriptional regulation of GLCE in human breast tissue. A common scheme for the regulation of GLCE expression in normal and tumor breast tissues is suggested.</abstract><cop>United States</cop><pub>Landes Bioscience</pub><pmid>22968430</pmid><doi>10.4161/epi.22103</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Binding Biology Bioscience biosynthesis breast cancer Breast Neoplasms - genetics Breast Neoplasms - metabolism Brief Report Calcium Cancer Carbohydrate Epimerases - genetics Carbohydrate Epimerases - metabolism Cell Cycle D-glucuronyl C5-epimerase expression Female Gene Expression Regulation, Neoplastic GLCE heparan sulphate proteoglycan Humans Landes MCF-7 Cells MicroRNAs - genetics MicroRNAs - metabolism miRNA Organogenesis Proteins RNA, Messenger - genetics TECHNOLOGY TEKNIKVETENSKAP tumor-suppressor gene |
| title | miRNA-218 contributes to the regulation of D-glucuronyl C5-epimerase expression in normal and tumor breast tissues |
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