A sensitive method based on fluorescence-detected circular dichroism for protein local structure analysis

We report an improved fluorescence-detected circular dichroism (FDCD)-based analytical method that is useful for probing protein three-dimensional structures. The method uses a novel FDCD device with an ellipsoidal mirror that functions on a standard circular dichroism (CD) spectrometer and eliminat...

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Veröffentlicht in:	Analytical biochemistry 2012-11, Vol.430 (2), p.179-184
Hauptverfasser:	Nehira, Tatsuo, Ishihara, Kaoru, Matsuo, Koichi, Izumi, Shunsuke, Yamazaki, Takeshi, Ishida, Atsuhiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bees calcium Calcium - metabolism Calmodulin Calmodulin - chemistry Calmodulin - metabolism Circular Dichroism circular dichroism spectroscopy Conformation denaturation fluorescence Fluorescence-detected circular dichroism fluorescent dyes Fluorescent Dyes - chemistry Horses Hydrogen-Ion Concentration Metmyoglobin Metmyoglobin - chemistry Metmyoglobin - metabolism peptides Peptides - analysis Protein Binding Protein Denaturation Protein Structure, Secondary Protein Structure, Tertiary Spectrometry, Fluorescence Trypsin - metabolism tryptophan
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