Improved gap repair cloning in yeast: treatment of the gapped vector with Taq DNA polymerase avoids vector self-ligation

Gap repair is a fast and efficient method for assembling recombinant DNA molecules in Saccharomyces cerevisiae. This method produces a circular DNA molecule by homologous recombination between two or more linear DNA fragments, one of which is typically a vector carrying replicative sequences and a s...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Yeast (Chichester, England) England), 2012-10, Vol.29 (10), p.419-423
Hauptverfasser:	Bessa, Daniela, Pereira, Filipa, Moreira, Roxana, Johansson, Björn, Queirós, Odília
Format:	Artikel
Sprache:	eng
Schlagworte:	Cloning, Molecular - methods DNA Repair - genetics DNA, Circular - genetics DNA, Fungal - genetics DNA, Recombinant - genetics gap repair Genetic Vectors homologous recombination Saccharomyces cerevisiae - genetics Taq polymerase Taq Polymerase - metabolism Yarrowia - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!