Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation
Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression...
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| Veröffentlicht in: | Experimental hematology 2012-11, Vol.40 (11), p.899-905.e5 |
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| creator | Verner, Jan Kabathova, Jitka Tomancova, Alexandra Pavlova, Sarka Tichy, Boris Mraz, Marek Brychtova, Yvona Krejci, Marta Zdrahal, Zbynek Trbusek, Martin Volejnikova, Jana Sedlacek, Petr Doubek, Michael Mayer, Jiri Pospisilova, Sarka |
| description | Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups—favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8 , G0S2 , ANXA3 , and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes. |
| doi_str_mv | 10.1016/j.exphem.2012.06.011 |
| format | Article |
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Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups—favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8 , G0S2 , ANXA3 , and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes.</description><identifier>ISSN: 0301-472X</identifier><identifier>EISSN: 1873-2399</identifier><identifier>DOI: 10.1016/j.exphem.2012.06.011</identifier><identifier>PMID: 22771791</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Acute Disease ; Adult ; Advanced Basic Science ; Child ; Child, Preschool ; Female ; Gene Expression Profiling ; Graft vs Host Disease - genetics ; Hematology, Oncology and Palliative Medicine ; Hematopoietic Stem Cell Transplantation ; Humans ; Male ; Middle Aged ; Survival Rate</subject><ispartof>Experimental hematology, 2012-11, Vol.40 (11), p.899-905.e5</ispartof><rights>ISEH - Society for Hematology and Stem Cells</rights><rights>2012 ISEH - Society for Hematology and Stem Cells</rights><rights>Copyright © 2012 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-9ddb85942f2e5630108ba58ce179ab0197f0f25a72e1d7fd947af5dd02bf7f483</citedby><cites>FETCH-LOGICAL-c463t-9ddb85942f2e5630108ba58ce179ab0197f0f25a72e1d7fd947af5dd02bf7f483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0301472X12002664$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22771791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verner, Jan</creatorcontrib><creatorcontrib>Kabathova, Jitka</creatorcontrib><creatorcontrib>Tomancova, Alexandra</creatorcontrib><creatorcontrib>Pavlova, Sarka</creatorcontrib><creatorcontrib>Tichy, Boris</creatorcontrib><creatorcontrib>Mraz, Marek</creatorcontrib><creatorcontrib>Brychtova, Yvona</creatorcontrib><creatorcontrib>Krejci, Marta</creatorcontrib><creatorcontrib>Zdrahal, Zbynek</creatorcontrib><creatorcontrib>Trbusek, Martin</creatorcontrib><creatorcontrib>Volejnikova, Jana</creatorcontrib><creatorcontrib>Sedlacek, Petr</creatorcontrib><creatorcontrib>Doubek, Michael</creatorcontrib><creatorcontrib>Mayer, Jiri</creatorcontrib><creatorcontrib>Pospisilova, Sarka</creatorcontrib><title>Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation</title><title>Experimental hematology</title><addtitle>Exp Hematol</addtitle><description>Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups—favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8 , G0S2 , ANXA3 , and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes.</description><subject>Acute Disease</subject><subject>Adult</subject><subject>Advanced Basic Science</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Graft vs Host Disease - genetics</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Survival Rate</subject><issn>0301-472X</issn><issn>1873-2399</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1TAQhC0Eoo_CP0DIRy4Ja8eJkwsSqqBUqsQBkLhZjr1u_UjiYDsV_fc4eoVDL5wsWTOzs98S8ppBzYB17441_l5vca45MF5DVwNjT8iB9bKpeDMMT8kBGmCVkPzHGXmR0hEA2naA5-SMcymZHNiBuEtckJakiCn5sNA1Bucnv9zQ4Kg2W0Z6E7XL1V2qbkPK1PqEOiEtfxhpKaBzWIPH7A1NGWdqcJpojnpJ66SXrHOJfUmeOT0lfPXwnpPvnz5-u_hcXX-5vLr4cF0Z0TW5Gqwd-3YQ3HFsu9Ie-lG3vcFSVo_ABunA8VZLjsxKZwchtWutBT466UTfnJO3p9yyxq8NU1azT3shvWDYkmKM8UaA4G2RipPUxJBSRKfW6Gcd7xUDtRNWR3UirHbCCjpVCBfbm4cJ2zij_Wf6i7QI3p8EWPa88xhVMh4Xg9ZHNFnZ4P834XGAKffwRk8_8R7TMWxxKQwVU6l41Nf9yvuRGQfgXSeaP1ZLpXQ</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Verner, Jan</creator><creator>Kabathova, Jitka</creator><creator>Tomancova, Alexandra</creator><creator>Pavlova, Sarka</creator><creator>Tichy, Boris</creator><creator>Mraz, Marek</creator><creator>Brychtova, Yvona</creator><creator>Krejci, Marta</creator><creator>Zdrahal, Zbynek</creator><creator>Trbusek, Martin</creator><creator>Volejnikova, Jana</creator><creator>Sedlacek, Petr</creator><creator>Doubek, Michael</creator><creator>Mayer, Jiri</creator><creator>Pospisilova, Sarka</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121101</creationdate><title>Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation</title><author>Verner, Jan ; Kabathova, Jitka ; Tomancova, Alexandra ; Pavlova, Sarka ; Tichy, Boris ; Mraz, Marek ; Brychtova, Yvona ; Krejci, Marta ; Zdrahal, Zbynek ; Trbusek, Martin ; Volejnikova, Jana ; Sedlacek, Petr ; Doubek, Michael ; Mayer, Jiri ; Pospisilova, Sarka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-9ddb85942f2e5630108ba58ce179ab0197f0f25a72e1d7fd947af5dd02bf7f483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acute Disease</topic><topic>Adult</topic><topic>Advanced Basic Science</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Graft vs Host Disease - genetics</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Hematopoietic Stem Cell Transplantation</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Survival Rate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verner, Jan</creatorcontrib><creatorcontrib>Kabathova, Jitka</creatorcontrib><creatorcontrib>Tomancova, Alexandra</creatorcontrib><creatorcontrib>Pavlova, Sarka</creatorcontrib><creatorcontrib>Tichy, Boris</creatorcontrib><creatorcontrib>Mraz, Marek</creatorcontrib><creatorcontrib>Brychtova, Yvona</creatorcontrib><creatorcontrib>Krejci, Marta</creatorcontrib><creatorcontrib>Zdrahal, Zbynek</creatorcontrib><creatorcontrib>Trbusek, Martin</creatorcontrib><creatorcontrib>Volejnikova, Jana</creatorcontrib><creatorcontrib>Sedlacek, Petr</creatorcontrib><creatorcontrib>Doubek, Michael</creatorcontrib><creatorcontrib>Mayer, Jiri</creatorcontrib><creatorcontrib>Pospisilova, Sarka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verner, Jan</au><au>Kabathova, Jitka</au><au>Tomancova, Alexandra</au><au>Pavlova, Sarka</au><au>Tichy, Boris</au><au>Mraz, Marek</au><au>Brychtova, Yvona</au><au>Krejci, Marta</au><au>Zdrahal, Zbynek</au><au>Trbusek, Martin</au><au>Volejnikova, Jana</au><au>Sedlacek, Petr</au><au>Doubek, Michael</au><au>Mayer, Jiri</au><au>Pospisilova, Sarka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation</atitle><jtitle>Experimental hematology</jtitle><addtitle>Exp Hematol</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>40</volume><issue>11</issue><spage>899</spage><epage>905.e5</epage><pages>899-905.e5</pages><issn>0301-472X</issn><eissn>1873-2399</eissn><abstract>Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups—favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8 , G0S2 , ANXA3 , and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>22771791</pmid><doi>10.1016/j.exphem.2012.06.011</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Experimental hematology, 2012-11, Vol.40 (11), p.899-905.e5 |
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| subjects | Acute Disease Adult Advanced Basic Science Child Child, Preschool Female Gene Expression Profiling Graft vs Host Disease - genetics Hematology, Oncology and Palliative Medicine Hematopoietic Stem Cell Transplantation Humans Male Middle Aged Survival Rate |
| title | Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation |
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