Identification and cellular location of glutamine synthetase in human sperm
Glutamine synthetase (GS) catalyzes the de novo synthesis of glutamine, an amino acid that has been shown to influence sperm motility in mammals. To date, no information is available about GS content in human sperm. In this study, we have characterized the presence and cellular location of GS in fre...
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| Veröffentlicht in: | Cell and tissue research 2012-10, Vol.350 (1), p.183-187 |
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| creator | Francou, Maria Manuela Hombrebueno, José R. De Juan, Joaquín |
| description | Glutamine synthetase (GS) catalyzes the de novo synthesis of glutamine, an amino acid that has been shown to influence sperm motility in mammals. To date, no information is available about GS content in human sperm. In this study, we have characterized the presence and cellular location of GS in fresh human normozoospermic samples. We have detected a single band corresponding to GS by Western blot. Confocal analysis has revealed GS immunoreactivity in the post-acrosomal head region. Moreover, double-labeling experiments with either F-actin or calicin have demonstrated GS confinement in the post-acrosomal region of the perinuclear theca. These data have been validated by a post-embedding ultra-structural study. The presence of GS in the post-acrosomal region of the perinuclear theca suggests that human sperm can carry out in glutamine synthesis. |
| doi_str_mv | 10.1007/s00441-012-1465-x |
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To date, no information is available about GS content in human sperm. In this study, we have characterized the presence and cellular location of GS in fresh human normozoospermic samples. We have detected a single band corresponding to GS by Western blot. Confocal analysis has revealed GS immunoreactivity in the post-acrosomal head region. Moreover, double-labeling experiments with either F-actin or calicin have demonstrated GS confinement in the post-acrosomal region of the perinuclear theca. These data have been validated by a post-embedding ultra-structural study. The presence of GS in the post-acrosomal region of the perinuclear theca suggests that human sperm can carry out in glutamine synthesis.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-012-1465-x</identifier><identifier>PMID: 22777743</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Enzymes ; Glutamate-Ammonia Ligase - metabolism ; Glutamate-Ammonia Ligase - ultrastructure ; Glutamine ; Human body ; Human Genetics ; Humans ; Ligases ; Male ; Mice ; Mice, Inbred C57BL ; Molecular Medicine ; Muscle proteins ; Protein Transport ; Proteomics ; Short Communication ; Sperm ; Spermatozoa ; Spermatozoa - cytology ; Spermatozoa - enzymology ; Spermatozoa - ultrastructure ; Tissue Extracts</subject><ispartof>Cell and tissue research, 2012-10, Vol.350 (1), p.183-187</ispartof><rights>Springer-Verlag 2012</rights><rights>COPYRIGHT 2012 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-7f290731d8a78dec1b255d7853473b6845d0f7a27ad8f6da59291e164912321b3</citedby><cites>FETCH-LOGICAL-c470t-7f290731d8a78dec1b255d7853473b6845d0f7a27ad8f6da59291e164912321b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-012-1465-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-012-1465-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22777743$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Francou, Maria Manuela</creatorcontrib><creatorcontrib>Hombrebueno, José R.</creatorcontrib><creatorcontrib>De Juan, Joaquín</creatorcontrib><title>Identification and cellular location of glutamine synthetase in human sperm</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>Glutamine synthetase (GS) catalyzes the de novo synthesis of glutamine, an amino acid that has been shown to influence sperm motility in mammals. To date, no information is available about GS content in human sperm. In this study, we have characterized the presence and cellular location of GS in fresh human normozoospermic samples. We have detected a single band corresponding to GS by Western blot. Confocal analysis has revealed GS immunoreactivity in the post-acrosomal head region. Moreover, double-labeling experiments with either F-actin or calicin have demonstrated GS confinement in the post-acrosomal region of the perinuclear theca. These data have been validated by a post-embedding ultra-structural study. The presence of GS in the post-acrosomal region of the perinuclear theca suggests that human sperm can carry out in glutamine synthesis.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Enzymes</subject><subject>Glutamate-Ammonia Ligase - metabolism</subject><subject>Glutamate-Ammonia Ligase - ultrastructure</subject><subject>Glutamine</subject><subject>Human body</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Ligases</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Medicine</subject><subject>Muscle proteins</subject><subject>Protein Transport</subject><subject>Proteomics</subject><subject>Short Communication</subject><subject>Sperm</subject><subject>Spermatozoa</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - enzymology</subject><subject>Spermatozoa - ultrastructure</subject><subject>Tissue Extracts</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kd9rFDEQx4Mo9qz-Ab7IglD6sm1-J_tYiq3Fgi8VfAu5zeQuZTc5k11o_3uz3Fla0clDYObzHWbmi9BHgs8Ixuq8YMw5aTGhLeFStA-v0IpwRluslX6NVphh2iopfx6hd6XcY1wp2b1FR5SqGpyt0LcbB3EKPvR2Cik2Nrqmh2GYB5ubIR2yyTebYZ7sGCI05TFOW5hsgSbEZjuPNjZlB3l8j954OxT4cPiP0Y-rL3eXX9vb79c3lxe3bc8VnlrlaYcVI05bpR30ZE2FcEoLxhVbS82Fw15ZqqzTXjorOtoRIJJ3hDJK1uwYne777nL6NUOZzBjKMrSNkOZiSA0tiVS6op__Qu_TnGOdzhCsieZU1DM8URs7gAnRpynbfmlqLpjgHWNSLNTZP6j6HIyhTxF8qPkXgpNngi3YYdqWVO9YT1pegmQP9jmVksGbXQ6jzY91SLM4bfZOm-q0WZw2D1Xz6bDZvB7BPSn-WFsBugdKLcUN5Oer_6_rbyILsD8</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Francou, Maria Manuela</creator><creator>Hombrebueno, José R.</creator><creator>De Juan, Joaquín</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20121001</creationdate><title>Identification and cellular location of glutamine synthetase in human sperm</title><author>Francou, Maria Manuela ; Hombrebueno, José R. ; De Juan, Joaquín</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-7f290731d8a78dec1b255d7853473b6845d0f7a27ad8f6da59291e164912321b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Western</topic><topic>Enzymes</topic><topic>Glutamate-Ammonia Ligase - metabolism</topic><topic>Glutamate-Ammonia Ligase - ultrastructure</topic><topic>Glutamine</topic><topic>Human body</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Ligases</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Medicine</topic><topic>Muscle proteins</topic><topic>Protein Transport</topic><topic>Proteomics</topic><topic>Short Communication</topic><topic>Sperm</topic><topic>Spermatozoa</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - enzymology</topic><topic>Spermatozoa - ultrastructure</topic><topic>Tissue Extracts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Francou, Maria Manuela</creatorcontrib><creatorcontrib>Hombrebueno, José R.</creatorcontrib><creatorcontrib>De Juan, Joaquín</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Francou, Maria Manuela</au><au>Hombrebueno, José R.</au><au>De Juan, Joaquín</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and cellular location of glutamine synthetase in human sperm</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>350</volume><issue>1</issue><spage>183</spage><epage>187</epage><pages>183-187</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Glutamine synthetase (GS) catalyzes the de novo synthesis of glutamine, an amino acid that has been shown to influence sperm motility in mammals. 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| subjects | Animals Biomedical and Life Sciences Biomedicine Blotting, Western Enzymes Glutamate-Ammonia Ligase - metabolism Glutamate-Ammonia Ligase - ultrastructure Glutamine Human body Human Genetics Humans Ligases Male Mice Mice, Inbred C57BL Molecular Medicine Muscle proteins Protein Transport Proteomics Short Communication Sperm Spermatozoa Spermatozoa - cytology Spermatozoa - enzymology Spermatozoa - ultrastructure Tissue Extracts |
| title | Identification and cellular location of glutamine synthetase in human sperm |
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