Chemotactic and Immunoregulatory Properties of Bone Cells are Modulated by Endotoxin-Stimulated Lymphocytes
In our study, we explored the bidirectional communication via soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an in vitro coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bo...
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| Veröffentlicht in: | Inflammation 2012-10, Vol.35 (5), p.1618-1631 |
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| creator | Cvija, Hrvoje Kovacic, Natasa Katavic, Vedran Ivcevic, Sanja Aguila, Hector Leonardo Marusic, Ana Grcevic, Danka |
| description | In our study, we explored the bidirectional communication
via
soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an
in vitro
coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblasts (OBL) and osteoclasts (OCL), and of bone cells to modulate cytokine and activation profile of endotoxin-stimulated lymphocytes were tested. LPS-pulsed lymphocytes enhanced OCL but inhibited OBL differentiation and increased the RANKL/OPG ratio, and, at the same time, upregulated chemotactic properties of bone cells, specifically CCL2, CCL5, and CXCL10 in OCL and CCL5 and CXCL13 in OBL. In parallel, bone cells had immunosuppressive effects by downregulating the lymphocyte expression of interleukin (IL)-1, IL-6, TNF-α and co-stimulatory molecules. OCL stimulated the production of osteoclastogenic cytokine RANKL in T lymphocytes. The anti-inflammatory effect, especially of OBL, suggests a possible compensatory mechanism to limit the inflammatory reaction during infection. |
| doi_str_mv | 10.1007/s10753-012-9477-y |
| format | Article |
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via
soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an
in vitro
coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblasts (OBL) and osteoclasts (OCL), and of bone cells to modulate cytokine and activation profile of endotoxin-stimulated lymphocytes were tested. LPS-pulsed lymphocytes enhanced OCL but inhibited OBL differentiation and increased the RANKL/OPG ratio, and, at the same time, upregulated chemotactic properties of bone cells, specifically CCL2, CCL5, and CXCL10 in OCL and CCL5 and CXCL13 in OBL. In parallel, bone cells had immunosuppressive effects by downregulating the lymphocyte expression of interleukin (IL)-1, IL-6, TNF-α and co-stimulatory molecules. OCL stimulated the production of osteoclastogenic cytokine RANKL in T lymphocytes. The anti-inflammatory effect, especially of OBL, suggests a possible compensatory mechanism to limit the inflammatory reaction during infection.</description><identifier>ISSN: 0360-3997</identifier><identifier>EISSN: 1573-2576</identifier><identifier>DOI: 10.1007/s10753-012-9477-y</identifier><identifier>PMID: 22699680</identifier><identifier>CODEN: INFLD4</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - metabolism ; Cell Differentiation ; Cells, Cultured ; Chemokine CCL2 - biosynthesis ; Chemokine CCL5 - biosynthesis ; Chemokine CXCL10 ; Chemokine CXCL13 - biosynthesis ; Female ; Immunology ; Inflammation ; Interleukin-1 - biosynthesis ; Interleukin-6 - biosynthesis ; Internal Medicine ; Lipopolysaccharides - immunology ; Lymphocyte Activation ; Lymphocytes - immunology ; Lymphocytes - metabolism ; Mice ; Mice, Inbred C57BL ; Osteoblasts - immunology ; Osteoblasts - metabolism ; Osteoclasts - immunology ; Osteoclasts - metabolism ; Osteoprotegerin - biosynthesis ; Pathology ; Pharmacology/Toxicology ; RANK Ligand - biosynthesis ; RANK Ligand - metabolism ; Rheumatology ; Tumor Necrosis Factor-alpha - biosynthesis ; Up-Regulation</subject><ispartof>Inflammation, 2012-10, Vol.35 (5), p.1618-1631</ispartof><rights>Springer Science+Business Media, LLC 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-f08db748aa1634eaff342fb34fde34009d4cd991ad2f1ecb4d893e207838971d3</citedby><cites>FETCH-LOGICAL-c405t-f08db748aa1634eaff342fb34fde34009d4cd991ad2f1ecb4d893e207838971d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10753-012-9477-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10753-012-9477-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22699680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cvija, Hrvoje</creatorcontrib><creatorcontrib>Kovacic, Natasa</creatorcontrib><creatorcontrib>Katavic, Vedran</creatorcontrib><creatorcontrib>Ivcevic, Sanja</creatorcontrib><creatorcontrib>Aguila, Hector Leonardo</creatorcontrib><creatorcontrib>Marusic, Ana</creatorcontrib><creatorcontrib>Grcevic, Danka</creatorcontrib><title>Chemotactic and Immunoregulatory Properties of Bone Cells are Modulated by Endotoxin-Stimulated Lymphocytes</title><title>Inflammation</title><addtitle>Inflammation</addtitle><addtitle>Inflammation</addtitle><description>In our study, we explored the bidirectional communication
via
soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an
in vitro
coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblasts (OBL) and osteoclasts (OCL), and of bone cells to modulate cytokine and activation profile of endotoxin-stimulated lymphocytes were tested. LPS-pulsed lymphocytes enhanced OCL but inhibited OBL differentiation and increased the RANKL/OPG ratio, and, at the same time, upregulated chemotactic properties of bone cells, specifically CCL2, CCL5, and CXCL10 in OCL and CCL5 and CXCL13 in OBL. In parallel, bone cells had immunosuppressive effects by downregulating the lymphocyte expression of interleukin (IL)-1, IL-6, TNF-α and co-stimulatory molecules. OCL stimulated the production of osteoclastogenic cytokine RANKL in T lymphocytes. The anti-inflammatory effect, especially of OBL, suggests a possible compensatory mechanism to limit the inflammatory reaction during infection.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Chemokine CCL2 - biosynthesis</subject><subject>Chemokine CCL5 - biosynthesis</subject><subject>Chemokine CXCL10</subject><subject>Chemokine CXCL13 - biosynthesis</subject><subject>Female</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Interleukin-1 - biosynthesis</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Internal Medicine</subject><subject>Lipopolysaccharides - immunology</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes - immunology</subject><subject>Lymphocytes - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Osteoblasts - immunology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteoclasts - immunology</subject><subject>Osteoclasts - metabolism</subject><subject>Osteoprotegerin - biosynthesis</subject><subject>Pathology</subject><subject>Pharmacology/Toxicology</subject><subject>RANK Ligand - biosynthesis</subject><subject>RANK Ligand - metabolism</subject><subject>Rheumatology</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Up-Regulation</subject><issn>0360-3997</issn><issn>1573-2576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqN0U9rFDEYBvAgFrtWP4AXCXjxMvrmz0ySoy7VFlZaqJ6HzORNO3VnsiYZ6Hx7s-5WRBA8BZLf-yThIeQVg3cMQL1PDFQtKmC8MlKpanlCVqxWouK1ap6SFYgGKmGMOiXPU7oHAG20eEZOOW-MaTSsyPf1HY4h2z4PPbWTo5fjOE8h4u28tTnEhV7HsMOYB0w0ePoxTEjXuN0maiPSL8HtHTraLfR8ciGHh2GqbvIwHvc3y7i7C_2SMb0gJ95uE748rmfk26fzr-uLanP1-XL9YVP1EupcedCuU1Jbyxoh0XovJPedkN6hkADGyd4Zw6zjnmHfSaeNQA5KC20Uc-KMvD3k7mL4MWPK7TikvrzZThjm1DIwQirJlfkPKooVGupC3_xF78Mcp_KRXwqYaLQuih1UH0NKEX27i8No41JQuy-tPZTWltLafWntUmZeH5PnbkT3e-KxpQL4AaRyNN1i_PPqf6X-BG1Lo1o</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Cvija, Hrvoje</creator><creator>Kovacic, Natasa</creator><creator>Katavic, Vedran</creator><creator>Ivcevic, Sanja</creator><creator>Aguila, Hector Leonardo</creator><creator>Marusic, Ana</creator><creator>Grcevic, Danka</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20121001</creationdate><title>Chemotactic and Immunoregulatory Properties of Bone Cells are Modulated by Endotoxin-Stimulated Lymphocytes</title><author>Cvija, Hrvoje ; Kovacic, Natasa ; Katavic, Vedran ; Ivcevic, Sanja ; Aguila, Hector Leonardo ; Marusic, Ana ; Grcevic, Danka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-f08db748aa1634eaff342fb34fde34009d4cd991ad2f1ecb4d893e207838971d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Chemokine CCL2 - biosynthesis</topic><topic>Chemokine CCL5 - biosynthesis</topic><topic>Chemokine CXCL10</topic><topic>Chemokine CXCL13 - biosynthesis</topic><topic>Female</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Interleukin-1 - biosynthesis</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Internal Medicine</topic><topic>Lipopolysaccharides - immunology</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes - immunology</topic><topic>Lymphocytes - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Osteoblasts - immunology</topic><topic>Osteoblasts - metabolism</topic><topic>Osteoclasts - immunology</topic><topic>Osteoclasts - metabolism</topic><topic>Osteoprotegerin - biosynthesis</topic><topic>Pathology</topic><topic>Pharmacology/Toxicology</topic><topic>RANK Ligand - biosynthesis</topic><topic>RANK Ligand - metabolism</topic><topic>Rheumatology</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cvija, Hrvoje</creatorcontrib><creatorcontrib>Kovacic, Natasa</creatorcontrib><creatorcontrib>Katavic, Vedran</creatorcontrib><creatorcontrib>Ivcevic, Sanja</creatorcontrib><creatorcontrib>Aguila, Hector Leonardo</creatorcontrib><creatorcontrib>Marusic, Ana</creatorcontrib><creatorcontrib>Grcevic, Danka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - 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via
soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an
in vitro
coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblasts (OBL) and osteoclasts (OCL), and of bone cells to modulate cytokine and activation profile of endotoxin-stimulated lymphocytes were tested. LPS-pulsed lymphocytes enhanced OCL but inhibited OBL differentiation and increased the RANKL/OPG ratio, and, at the same time, upregulated chemotactic properties of bone cells, specifically CCL2, CCL5, and CXCL10 in OCL and CCL5 and CXCL13 in OBL. In parallel, bone cells had immunosuppressive effects by downregulating the lymphocyte expression of interleukin (IL)-1, IL-6, TNF-α and co-stimulatory molecules. OCL stimulated the production of osteoclastogenic cytokine RANKL in T lymphocytes. The anti-inflammatory effect, especially of OBL, suggests a possible compensatory mechanism to limit the inflammatory reaction during infection.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>22699680</pmid><doi>10.1007/s10753-012-9477-y</doi><tpages>14</tpages></addata></record> |
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| subjects | Animals Biomedical and Life Sciences Biomedicine Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Bone Marrow Cells - metabolism Cell Differentiation Cells, Cultured Chemokine CCL2 - biosynthesis Chemokine CCL5 - biosynthesis Chemokine CXCL10 Chemokine CXCL13 - biosynthesis Female Immunology Inflammation Interleukin-1 - biosynthesis Interleukin-6 - biosynthesis Internal Medicine Lipopolysaccharides - immunology Lymphocyte Activation Lymphocytes - immunology Lymphocytes - metabolism Mice Mice, Inbred C57BL Osteoblasts - immunology Osteoblasts - metabolism Osteoclasts - immunology Osteoclasts - metabolism Osteoprotegerin - biosynthesis Pathology Pharmacology/Toxicology RANK Ligand - biosynthesis RANK Ligand - metabolism Rheumatology Tumor Necrosis Factor-alpha - biosynthesis Up-Regulation |
| title | Chemotactic and Immunoregulatory Properties of Bone Cells are Modulated by Endotoxin-Stimulated Lymphocytes |
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