ddm1 plants are sensitive to methyl methane sulfonate and NaCl stresses and are deficient in DNA repair
Plant response to stress includes changes in gene expression and chromatin structure. Our previous work showed that Arabidopsis thaliana Dicer-like (DCL) mutants were impaired in transgenerational response to stress that included an increase in recombination frequency, cytosine methylation and stres...
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| Veröffentlicht in: | Plant cell reports 2012-09, Vol.31 (9), p.1549-1561 |
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| creator | Yao, Youli Bilichak, Andriy Golubov, Andrey Kovalchuk, Igor |
| description | Plant response to stress includes changes in gene expression and chromatin structure. Our previous work showed that Arabidopsis thaliana Dicer-like (DCL) mutants were impaired in transgenerational response to stress that included an increase in recombination frequency, cytosine methylation and stress tolerance. It can be hypothesized that changes in chromatin structure are important for an efficient stress response. To test this hypothesis, we analyzed the stress response of ddm1, a mutant impaired in DDM1, a member of the SWI/SNF family of adenosine triphosphate-dependent chromatin remodeling genes. We exposed Arabidopsis thaliana ddm1 mutants to methyl methane sulfonate (MMS) and NaCl and found that these plants were more sensitive. At the same time, ddm1 plants were similar to wild-type plants in sensitivity to temperature and bleomycin stresses. Direct comparison to met1 plants, deficient in maintenance methyltransferase MET1, showed higher sensitivity of ddm1 plants to NaCl. The level of DNA strand breaks upon exposure to MMS increased in wild-type plants but decreased in ddm1 plants. DNA methylation analysis showed that heterozygous ddm1/DDM1 plants had lower methylation as compared to fourth generation of homozygous ddm1/ddm1 plants. Exposure to MMS resulted in a decrease in methylation in wild-type plants and an increase in ddm1 plants. Finally, in vitro DNA excision repair assay showed lower capacity for ddm1 mutant. Our results provided a new example of a link between genetic genome stability and epigenetic genome stability. Key message We demonstrate that heterozygous ddm1/DDM1 plants are more sensitive to stress and have more severe changes in methylation than homozygous ddm1/ddm1 plants. |
| doi_str_mv | 10.1007/s00299-012-1269-1 |
| format | Article |
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Our previous work showed that Arabidopsis thaliana Dicer-like (DCL) mutants were impaired in transgenerational response to stress that included an increase in recombination frequency, cytosine methylation and stress tolerance. It can be hypothesized that changes in chromatin structure are important for an efficient stress response. To test this hypothesis, we analyzed the stress response of ddm1, a mutant impaired in DDM1, a member of the SWI/SNF family of adenosine triphosphate-dependent chromatin remodeling genes. We exposed Arabidopsis thaliana ddm1 mutants to methyl methane sulfonate (MMS) and NaCl and found that these plants were more sensitive. At the same time, ddm1 plants were similar to wild-type plants in sensitivity to temperature and bleomycin stresses. Direct comparison to met1 plants, deficient in maintenance methyltransferase MET1, showed higher sensitivity of ddm1 plants to NaCl. The level of DNA strand breaks upon exposure to MMS increased in wild-type plants but decreased in ddm1 plants. DNA methylation analysis showed that heterozygous ddm1/DDM1 plants had lower methylation as compared to fourth generation of homozygous ddm1/ddm1 plants. Exposure to MMS resulted in a decrease in methylation in wild-type plants and an increase in ddm1 plants. Finally, in vitro DNA excision repair assay showed lower capacity for ddm1 mutant. Our results provided a new example of a link between genetic genome stability and epigenetic genome stability. Key message We demonstrate that heterozygous ddm1/DDM1 plants are more sensitive to stress and have more severe changes in methylation than homozygous ddm1/ddm1 plants.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-012-1269-1</identifier><identifier>PMID: 22538524</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Adaptation, Physiological - drug effects ; adenosine ; Arabidopsis - drug effects ; Arabidopsis - physiology ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; ATP ; Biological and medical sciences ; Biological Assay ; Biomedical and Life Sciences ; Biotechnology ; Bleomycin ; Bleomycin - pharmacology ; Cell Biology ; chromatin ; Chromatin remodeling ; Crosses, Genetic ; Cytosine ; Deoxyribonucleic acid ; DNA ; DNA (Cytosine-5-)-Methyltransferases - metabolism ; DNA Breaks, Double-Stranded - drug effects ; DNA damage ; DNA methylation ; DNA Methylation - drug effects ; DNA Methylation - genetics ; DNA repair ; DNA Repair - drug effects ; DNA-Binding Proteins - metabolism ; epigenetics ; Fundamental and applied biological sciences. Psychology ; Gene expression ; gene expression regulation ; genes ; Genomes ; Heterozygote ; Life Sciences ; Methane ; methyl methanesulfonate ; Methyl Methanesulfonate - pharmacology ; Methyltransferase ; Mutants ; Mutation - genetics ; Original Paper ; Plant Biochemistry ; plant response ; Plant Sciences ; Recombination ; Reproducibility of Results ; Sodium chloride ; Sodium Chloride - pharmacology ; Stress ; stress response ; stress tolerance ; Stress, Physiological - drug effects ; Sulfonates ; Temperature ; Temperature effects ; Transcription Factors - metabolism</subject><ispartof>Plant cell reports, 2012-09, Vol.31 (9), p.1549-1561</ispartof><rights>Springer-Verlag 2012</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-c48f96a64e1114168274673d72846c83bf0145ae8781a389b3591f073b5bb9b53</citedby><cites>FETCH-LOGICAL-c459t-c48f96a64e1114168274673d72846c83bf0145ae8781a389b3591f073b5bb9b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-012-1269-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-012-1269-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26225001$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22538524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yao, Youli</creatorcontrib><creatorcontrib>Bilichak, Andriy</creatorcontrib><creatorcontrib>Golubov, Andrey</creatorcontrib><creatorcontrib>Kovalchuk, Igor</creatorcontrib><title>ddm1 plants are sensitive to methyl methane sulfonate and NaCl stresses and are deficient in DNA repair</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Plant response to stress includes changes in gene expression and chromatin structure. Our previous work showed that Arabidopsis thaliana Dicer-like (DCL) mutants were impaired in transgenerational response to stress that included an increase in recombination frequency, cytosine methylation and stress tolerance. It can be hypothesized that changes in chromatin structure are important for an efficient stress response. To test this hypothesis, we analyzed the stress response of ddm1, a mutant impaired in DDM1, a member of the SWI/SNF family of adenosine triphosphate-dependent chromatin remodeling genes. We exposed Arabidopsis thaliana ddm1 mutants to methyl methane sulfonate (MMS) and NaCl and found that these plants were more sensitive. At the same time, ddm1 plants were similar to wild-type plants in sensitivity to temperature and bleomycin stresses. Direct comparison to met1 plants, deficient in maintenance methyltransferase MET1, showed higher sensitivity of ddm1 plants to NaCl. The level of DNA strand breaks upon exposure to MMS increased in wild-type plants but decreased in ddm1 plants. DNA methylation analysis showed that heterozygous ddm1/DDM1 plants had lower methylation as compared to fourth generation of homozygous ddm1/ddm1 plants. Exposure to MMS resulted in a decrease in methylation in wild-type plants and an increase in ddm1 plants. Finally, in vitro DNA excision repair assay showed lower capacity for ddm1 mutant. Our results provided a new example of a link between genetic genome stability and epigenetic genome stability. Key message We demonstrate that heterozygous ddm1/DDM1 plants are more sensitive to stress and have more severe changes in methylation than homozygous ddm1/ddm1 plants.</description><subject>Adaptation, Physiological - drug effects</subject><subject>adenosine</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Bleomycin</subject><subject>Bleomycin - pharmacology</subject><subject>Cell Biology</subject><subject>chromatin</subject><subject>Chromatin remodeling</subject><subject>Crosses, Genetic</subject><subject>Cytosine</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA (Cytosine-5-)-Methyltransferases - metabolism</subject><subject>DNA Breaks, Double-Stranded - drug effects</subject><subject>DNA damage</subject><subject>DNA methylation</subject><subject>DNA Methylation - drug effects</subject><subject>DNA Methylation - genetics</subject><subject>DNA repair</subject><subject>DNA Repair - drug effects</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>epigenetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>Genomes</subject><subject>Heterozygote</subject><subject>Life Sciences</subject><subject>Methane</subject><subject>methyl methanesulfonate</subject><subject>Methyl Methanesulfonate - pharmacology</subject><subject>Methyltransferase</subject><subject>Mutants</subject><subject>Mutation - genetics</subject><subject>Original Paper</subject><subject>Plant Biochemistry</subject><subject>plant response</subject><subject>Plant Sciences</subject><subject>Recombination</subject><subject>Reproducibility of Results</subject><subject>Sodium chloride</subject><subject>Sodium Chloride - pharmacology</subject><subject>Stress</subject><subject>stress response</subject><subject>stress tolerance</subject><subject>Stress, Physiological - drug effects</subject><subject>Sulfonates</subject><subject>Temperature</subject><subject>Temperature effects</subject><subject>Transcription Factors - metabolism</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kUtv1TAQhS0EoreFH8AGLFWV2AT8ih0vq8tTqsoCKrGLnGRySZU4F4-D1H_P3ObyEBIbj-T5zvF4DmPPpHglhXCvUQjlfSGkKqSyvpAP2EYarQol9NeHbCOckoVz0pywU8RbIajp7GN2olSpq1KZDdt13ST5fgwxIw8JOELEIQ8_gOeZT5C_3Y33JUTqLWM_x5CBh9jx67AdOeYEiID3Nwd9B_3QDhAzHyJ_c33JE-zDkJ6wR30YEZ4e6xm7eff2y_ZDcfXp_cft5VXRmtJnOqve22ANSCmNtJVyxjrdOVUZ21a66ekPZYDKVTLoyje69LIXTjdl0_im1Gfs5eq7T_P3BTDX04AtjPRBmBespfDaWCOFIfT8H_R2XlKk6YjSjhbqnCZKrlSbZsQEfb1PwxTSHUH1IYV6TaGmFOpDCrUkzfOj89JM0P1W_Fo7ARdHIGAbxj6F2A74h7NEUlrEqZVDasUdpL9H_P_rL1ZRH-Y67BIZ33xWtDdBlt4qr38CbvulsA</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Yao, Youli</creator><creator>Bilichak, Andriy</creator><creator>Golubov, Andrey</creator><creator>Kovalchuk, Igor</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20120901</creationdate><title>ddm1 plants are sensitive to methyl methane sulfonate and NaCl stresses and are deficient in DNA repair</title><author>Yao, Youli ; Bilichak, Andriy ; Golubov, Andrey ; Kovalchuk, Igor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-c48f96a64e1114168274673d72846c83bf0145ae8781a389b3591f073b5bb9b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adaptation, Physiological - drug effects</topic><topic>adenosine</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>ATP</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Bleomycin</topic><topic>Bleomycin - pharmacology</topic><topic>Cell Biology</topic><topic>chromatin</topic><topic>Chromatin remodeling</topic><topic>Crosses, Genetic</topic><topic>Cytosine</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA (Cytosine-5-)-Methyltransferases - metabolism</topic><topic>DNA Breaks, Double-Stranded - drug effects</topic><topic>DNA damage</topic><topic>DNA methylation</topic><topic>DNA Methylation - drug effects</topic><topic>DNA Methylation - genetics</topic><topic>DNA repair</topic><topic>DNA Repair - drug effects</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>epigenetics</topic><topic>Fundamental and applied biological sciences. 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Rep</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>31</volume><issue>9</issue><spage>1549</spage><epage>1561</epage><pages>1549-1561</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Plant response to stress includes changes in gene expression and chromatin structure. Our previous work showed that Arabidopsis thaliana Dicer-like (DCL) mutants were impaired in transgenerational response to stress that included an increase in recombination frequency, cytosine methylation and stress tolerance. It can be hypothesized that changes in chromatin structure are important for an efficient stress response. To test this hypothesis, we analyzed the stress response of ddm1, a mutant impaired in DDM1, a member of the SWI/SNF family of adenosine triphosphate-dependent chromatin remodeling genes. We exposed Arabidopsis thaliana ddm1 mutants to methyl methane sulfonate (MMS) and NaCl and found that these plants were more sensitive. At the same time, ddm1 plants were similar to wild-type plants in sensitivity to temperature and bleomycin stresses. Direct comparison to met1 plants, deficient in maintenance methyltransferase MET1, showed higher sensitivity of ddm1 plants to NaCl. The level of DNA strand breaks upon exposure to MMS increased in wild-type plants but decreased in ddm1 plants. DNA methylation analysis showed that heterozygous ddm1/DDM1 plants had lower methylation as compared to fourth generation of homozygous ddm1/ddm1 plants. Exposure to MMS resulted in a decrease in methylation in wild-type plants and an increase in ddm1 plants. Finally, in vitro DNA excision repair assay showed lower capacity for ddm1 mutant. Our results provided a new example of a link between genetic genome stability and epigenetic genome stability. Key message We demonstrate that heterozygous ddm1/DDM1 plants are more sensitive to stress and have more severe changes in methylation than homozygous ddm1/ddm1 plants.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22538524</pmid><doi>10.1007/s00299-012-1269-1</doi><tpages>13</tpages></addata></record> |
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| subjects | Adaptation, Physiological - drug effects adenosine Arabidopsis - drug effects Arabidopsis - physiology Arabidopsis Proteins - metabolism Arabidopsis thaliana ATP Biological and medical sciences Biological Assay Biomedical and Life Sciences Biotechnology Bleomycin Bleomycin - pharmacology Cell Biology chromatin Chromatin remodeling Crosses, Genetic Cytosine Deoxyribonucleic acid DNA DNA (Cytosine-5-)-Methyltransferases - metabolism DNA Breaks, Double-Stranded - drug effects DNA damage DNA methylation DNA Methylation - drug effects DNA Methylation - genetics DNA repair DNA Repair - drug effects DNA-Binding Proteins - metabolism epigenetics Fundamental and applied biological sciences. Psychology Gene expression gene expression regulation genes Genomes Heterozygote Life Sciences Methane methyl methanesulfonate Methyl Methanesulfonate - pharmacology Methyltransferase Mutants Mutation - genetics Original Paper Plant Biochemistry plant response Plant Sciences Recombination Reproducibility of Results Sodium chloride Sodium Chloride - pharmacology Stress stress response stress tolerance Stress, Physiological - drug effects Sulfonates Temperature Temperature effects Transcription Factors - metabolism |
| title | ddm1 plants are sensitive to methyl methane sulfonate and NaCl stresses and are deficient in DNA repair |
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