Coat protein-mediated transgenic resistance in tomato against a IB subgroup Cucumber mosaic virus strain
Transgenic tomato plants containing the coat protein (CP) gene of Cucumber mosaic virus (CMV) of subgroup IB were developed through Agrobacterium- mediated transformations. The progenies of transgenic plants showed the presence of transgene, its expression and translation of 26 KDa CP. The T 1 and T...
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| Veröffentlicht in: | Phytoparasitica 2012-09, Vol.40 (4), p.375-382 |
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| container_title | Phytoparasitica |
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| creator | Pratap, D. Raj, S. K. Kumar, S. Snehi, S. K. Gautam, K. K. Sharma, A. K. |
| description | Transgenic tomato plants containing the coat protein (CP) gene of
Cucumber mosaic virus
(CMV) of subgroup IB were developed through
Agrobacterium-
mediated transformations. The progenies of transgenic plants showed the presence of transgene, its expression and translation of 26 KDa CP. The T
1
and T
2
generation plants were evaluated for resistance against challenge inoculations by a homologous strain of CMV. Visual observations of challenged transgenic plants categorized them into resistant, tolerant and susceptible as compared with untransformed control plants. Out of 33 plants of the T
1
generation, 36.3% showed resistance and remained symptomless throughout their life, 48.4% showed tolerance which developed delayed symptoms of mild mosaic, and 15.1% showed susceptibility to CMV which developed severe systemic mosaic and leaf distortion symptoms after 30 days of virus challenge. Out of 120 plants of the T
2
generation, 60% showed resistance, 26.6% were tolerant and only 13.3% were found susceptible to challenge inoculations of CMV. Resistant transgenic plants also showed less CP accumulation in systemic upper leaves as compared with challenged untransformed plants. In this study, CP of a CMV subgroup IB strain has demonstrated a significant level of resistance in transgenic tomato plants against the CMV strain. The strategy may be applied for better quality and productivity of tomato crops. |
| doi_str_mv | 10.1007/s12600-012-0238-0 |
| format | Article |
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Cucumber mosaic virus
(CMV) of subgroup IB were developed through
Agrobacterium-
mediated transformations. The progenies of transgenic plants showed the presence of transgene, its expression and translation of 26 KDa CP. The T
1
and T
2
generation plants were evaluated for resistance against challenge inoculations by a homologous strain of CMV. Visual observations of challenged transgenic plants categorized them into resistant, tolerant and susceptible as compared with untransformed control plants. Out of 33 plants of the T
1
generation, 36.3% showed resistance and remained symptomless throughout their life, 48.4% showed tolerance which developed delayed symptoms of mild mosaic, and 15.1% showed susceptibility to CMV which developed severe systemic mosaic and leaf distortion symptoms after 30 days of virus challenge. Out of 120 plants of the T
2
generation, 60% showed resistance, 26.6% were tolerant and only 13.3% were found susceptible to challenge inoculations of CMV. Resistant transgenic plants also showed less CP accumulation in systemic upper leaves as compared with challenged untransformed plants. In this study, CP of a CMV subgroup IB strain has demonstrated a significant level of resistance in transgenic tomato plants against the CMV strain. The strategy may be applied for better quality and productivity of tomato crops.</description><identifier>ISSN: 0334-2123</identifier><identifier>EISSN: 1876-7184</identifier><identifier>DOI: 10.1007/s12600-012-0238-0</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Biomedical and Life Sciences ; Cucumber mosaic virus ; Cytomegalovirus ; Ecology ; Leaves ; Life Sciences ; Lycopersicon esculentum ; Plant diseases ; Plant Pathology ; Plant Sciences ; Proteins ; Resistance to control ; Tomatoes ; Transgenic plants ; Viruses</subject><ispartof>Phytoparasitica, 2012-09, Vol.40 (4), p.375-382</ispartof><rights>Springer Science + Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-14491d8dd46efc8bd990bc031fcfe88ab3a18c23270462cee4aa6f2fdaef3b273</citedby><cites>FETCH-LOGICAL-c349t-14491d8dd46efc8bd990bc031fcfe88ab3a18c23270462cee4aa6f2fdaef3b273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12600-012-0238-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12600-012-0238-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Pratap, D.</creatorcontrib><creatorcontrib>Raj, S. K.</creatorcontrib><creatorcontrib>Kumar, S.</creatorcontrib><creatorcontrib>Snehi, S. K.</creatorcontrib><creatorcontrib>Gautam, K. K.</creatorcontrib><creatorcontrib>Sharma, A. K.</creatorcontrib><title>Coat protein-mediated transgenic resistance in tomato against a IB subgroup Cucumber mosaic virus strain</title><title>Phytoparasitica</title><addtitle>Phytoparasitica</addtitle><description>Transgenic tomato plants containing the coat protein (CP) gene of
Cucumber mosaic virus
(CMV) of subgroup IB were developed through
Agrobacterium-
mediated transformations. The progenies of transgenic plants showed the presence of transgene, its expression and translation of 26 KDa CP. The T
1
and T
2
generation plants were evaluated for resistance against challenge inoculations by a homologous strain of CMV. Visual observations of challenged transgenic plants categorized them into resistant, tolerant and susceptible as compared with untransformed control plants. Out of 33 plants of the T
1
generation, 36.3% showed resistance and remained symptomless throughout their life, 48.4% showed tolerance which developed delayed symptoms of mild mosaic, and 15.1% showed susceptibility to CMV which developed severe systemic mosaic and leaf distortion symptoms after 30 days of virus challenge. Out of 120 plants of the T
2
generation, 60% showed resistance, 26.6% were tolerant and only 13.3% were found susceptible to challenge inoculations of CMV. Resistant transgenic plants also showed less CP accumulation in systemic upper leaves as compared with challenged untransformed plants. In this study, CP of a CMV subgroup IB strain has demonstrated a significant level of resistance in transgenic tomato plants against the CMV strain. The strategy may be applied for better quality and productivity of tomato crops.</description><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Cucumber mosaic virus</subject><subject>Cytomegalovirus</subject><subject>Ecology</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Lycopersicon esculentum</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Proteins</subject><subject>Resistance to control</subject><subject>Tomatoes</subject><subject>Transgenic plants</subject><subject>Viruses</subject><issn>0334-2123</issn><issn>1876-7184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kMtqHDEQRYVxwGM7H-CdIJtsFJce9GOZDHkYDN7Y66ZaXT2WmZYmKnXAfx8N40UIeFWbc25VXSFuNHzRAO0ta9MAKNBGgbGdgjOx0V3bqFZ37lxswFqnjDb2QlwyvwBUWsNGPG8TFnnIqVCIaqEpYKFJloyRdxSDl5k4cMHoSYYoS1qwJIk7DJGLRHn3TfI67nJaD3K7-nUZKcslMVb1T8grS65hIV6LDzPumT6-zSvx9OP74_aXun_4ebf9eq-8dX1R2rleT900uYZm341T38PowerZz9R1OFrUnTfWtOAa44kcYjObeUKa7WhaeyU-n3LrT79X4jIsgT3t9xgprTxo6K1rLPRH9NN_6Etac6zXVcq2VtclR0qfKJ8Tc6Z5OOSwYH6t0HDsfjh1P9Tuh2P3A1THnByubNxR_jf5PekvDySIGA</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Pratap, D.</creator><creator>Raj, S. 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K. ; Kumar, S. ; Snehi, S. K. ; Gautam, K. K. ; Sharma, A. K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-14491d8dd46efc8bd990bc031fcfe88ab3a18c23270462cee4aa6f2fdaef3b273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agriculture</topic><topic>Biomedical and Life Sciences</topic><topic>Cucumber mosaic virus</topic><topic>Cytomegalovirus</topic><topic>Ecology</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Lycopersicon esculentum</topic><topic>Plant diseases</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Proteins</topic><topic>Resistance to control</topic><topic>Tomatoes</topic><topic>Transgenic plants</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pratap, D.</creatorcontrib><creatorcontrib>Raj, S. K.</creatorcontrib><creatorcontrib>Kumar, S.</creatorcontrib><creatorcontrib>Snehi, S. K.</creatorcontrib><creatorcontrib>Gautam, K. K.</creatorcontrib><creatorcontrib>Sharma, A. K.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Phytoparasitica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pratap, D.</au><au>Raj, S. K.</au><au>Kumar, S.</au><au>Snehi, S. K.</au><au>Gautam, K. K.</au><au>Sharma, A. K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coat protein-mediated transgenic resistance in tomato against a IB subgroup Cucumber mosaic virus strain</atitle><jtitle>Phytoparasitica</jtitle><stitle>Phytoparasitica</stitle><date>2012-09-01</date><risdate>2012</risdate><volume>40</volume><issue>4</issue><spage>375</spage><epage>382</epage><pages>375-382</pages><issn>0334-2123</issn><eissn>1876-7184</eissn><abstract>Transgenic tomato plants containing the coat protein (CP) gene of
Cucumber mosaic virus
(CMV) of subgroup IB were developed through
Agrobacterium-
mediated transformations. The progenies of transgenic plants showed the presence of transgene, its expression and translation of 26 KDa CP. The T
1
and T
2
generation plants were evaluated for resistance against challenge inoculations by a homologous strain of CMV. Visual observations of challenged transgenic plants categorized them into resistant, tolerant and susceptible as compared with untransformed control plants. Out of 33 plants of the T
1
generation, 36.3% showed resistance and remained symptomless throughout their life, 48.4% showed tolerance which developed delayed symptoms of mild mosaic, and 15.1% showed susceptibility to CMV which developed severe systemic mosaic and leaf distortion symptoms after 30 days of virus challenge. Out of 120 plants of the T
2
generation, 60% showed resistance, 26.6% were tolerant and only 13.3% were found susceptible to challenge inoculations of CMV. Resistant transgenic plants also showed less CP accumulation in systemic upper leaves as compared with challenged untransformed plants. In this study, CP of a CMV subgroup IB strain has demonstrated a significant level of resistance in transgenic tomato plants against the CMV strain. The strategy may be applied for better quality and productivity of tomato crops.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12600-012-0238-0</doi><tpages>8</tpages></addata></record> |
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| subjects | Agriculture Biomedical and Life Sciences Cucumber mosaic virus Cytomegalovirus Ecology Leaves Life Sciences Lycopersicon esculentum Plant diseases Plant Pathology Plant Sciences Proteins Resistance to control Tomatoes Transgenic plants Viruses |
| title | Coat protein-mediated transgenic resistance in tomato against a IB subgroup Cucumber mosaic virus strain |
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