Solid-phase extraction and GC-MS analysis of potentially genotoxic cleavage products of Delta b-carotene in primary cell cultures

Solid-phase extraction and GC-MS analysis of potentially genotoxic cleavage products of Delta b-carotene in primary cell cultures

A validated method for the simultaneous determination of prominent volatile cleavage products (CPs) of Delta *b-carotene in cell culture media has been developed. Target CPs comprised Delta *b-ionone ( Delta *b-IO), cyclocitral (CC), dihydroactinidiolide (DHA), and 1,1,6-trimethyltetraline (TMT). CP...

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Veröffentlicht in:Analytical and bioanalytical chemistry 2011-06, Vol.400 (8), p.2415-2426
Hauptverfasser: Martano, G, Vogl, C, Bojaxhi, E, Bresgen, N, Eckl, P, Stutz, H
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container_issue 8
container_start_page 2415
container_title Analytical and bioanalytical chemistry
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creator Martano, G
Vogl, C
Bojaxhi, E
Bresgen, N
Eckl, P
Stutz, H
description A validated method for the simultaneous determination of prominent volatile cleavage products (CPs) of Delta *b-carotene in cell culture media has been developed. Target CPs comprised Delta *b-ionone ( Delta *b-IO), cyclocitral (CC), dihydroactinidiolide (DHA), and 1,1,6-trimethyltetraline (TMT). CPs were extracted by solid-phase extraction applying a phenyl adsorbent, eluted with 10% (v/v) tetrahydrofuran in n-hexane, and identified and quantified by gas chromatography-mass spectrometry with electron impact ionization. Method validation addressed linearity confirmation over two application ranges and homoscedasticity testing. Recoveries from culture media were between 71.7% and 95.7% at 1.0 Delta *mg/ml. Precision of recoveries determined in intra-day (N=5) and inter-day (N=15) assays were
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