Effect of thymol on Ca super(2+ homeostasis and viability in human glioblastoma cells)
The effect of the natural essential oil thymol on cytosolic Ca super(2+ concentrations ([Ca) super(2)+] sub(i) and viability in human glioblastoma cells was examined. The Ca) super(2)+-sensitive fluorescent dye fura-2 was applied to measure [Ca super(2+]) sub(i). Thymol at concentrations of 400-1000...
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| Veröffentlicht in: | European journal of pharmacology 2011-11, Vol.670 (1), p.85-91 |
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| creator | Hsu, Shu-Shong Lin, Ko-Long Chou, Chiang-Ting Chiang, An-Jen Liang, Wei-Zhe Chang, Hong-Tai Tsai, Jeng-Yu Liao, Wei-Chuan Huang, Fong-Dee Huang, Jong Khing Chen, I-Shu Liu, Shuih-Inn Kuo, Chun-Chi Jan, Chung-Ren |
| description | The effect of the natural essential oil thymol on cytosolic Ca super(2+ concentrations ([Ca) super(2)+] sub(i) and viability in human glioblastoma cells was examined. The Ca) super(2)+-sensitive fluorescent dye fura-2 was applied to measure [Ca super(2+]) sub(i). Thymol at concentrations of 400-1000 mu M induced a [Ca super(2+]) sub(i) rise in a concentration-dependent fashion. The response was decreased partially by removal of extracellular Ca super(2+. Thymol-induced Ca) super(2)+ signal was not altered by nifedipine, econazole, SK&F96365, and protein kinase C activator phorbol myristate acetate (PMA), but was inhibited by the protein kinase C inhibitor GF109203X. When extracellular Ca super(2+ was removed, incubation with the endoplasmic reticulum Ca) super(2)+ pump inhibitor thapsigargin or 2,5-di-tert-butylhydroquinone (BHQ) abolished thymol-induced [Ca super(2+]) sub(i) rise. Incubation with thymol also abolished thapsigargin or BHQ-induced [Ca super(2+]) sub(i) rise. Inhibition of phospholipase C with U73122 abolished thymol-induced [Ca super(2+]) sub(i) rise. At concentrations of 200-800 mu M, thymol killed cells in a concentration-dependent manner. This cytotoxic effect was not changed by chelating cytosolic Ca super(2+ with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl (BAPTA/AM). Annexin V/propidium iodide staining data suggest that thymol (200, 400 and 600 mu M) induced apoptosis in a concentration-dependent manner. Collectively, in human glioblastoma cells, thymol induced a [Ca) super(2)+] sub(i rise by inducing phospholipase C- and protein kinase C-dependent Ca) super(2)+ release from the endoplasmic reticulum and Ca super(2+ entry via non store-operated Ca) super(2)+ channels. Thymol induced cell death that may involve apoptosis. |
| doi_str_mv | 10.1016/j.ejphar.2011.08.017 |
| format | Article |
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The Ca) super(2)+-sensitive fluorescent dye fura-2 was applied to measure [Ca super(2+]) sub(i). Thymol at concentrations of 400-1000 mu M induced a [Ca super(2+]) sub(i) rise in a concentration-dependent fashion. The response was decreased partially by removal of extracellular Ca super(2+. Thymol-induced Ca) super(2)+ signal was not altered by nifedipine, econazole, SK&F96365, and protein kinase C activator phorbol myristate acetate (PMA), but was inhibited by the protein kinase C inhibitor GF109203X. When extracellular Ca super(2+ was removed, incubation with the endoplasmic reticulum Ca) super(2)+ pump inhibitor thapsigargin or 2,5-di-tert-butylhydroquinone (BHQ) abolished thymol-induced [Ca super(2+]) sub(i) rise. Incubation with thymol also abolished thapsigargin or BHQ-induced [Ca super(2+]) sub(i) rise. Inhibition of phospholipase C with U73122 abolished thymol-induced [Ca super(2+]) sub(i) rise. At concentrations of 200-800 mu M, thymol killed cells in a concentration-dependent manner. This cytotoxic effect was not changed by chelating cytosolic Ca super(2+ with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl (BAPTA/AM). Annexin V/propidium iodide staining data suggest that thymol (200, 400 and 600 mu M) induced apoptosis in a concentration-dependent manner. Collectively, in human glioblastoma cells, thymol induced a [Ca) super(2)+] sub(i rise by inducing phospholipase C- and protein kinase C-dependent Ca) super(2)+ release from the endoplasmic reticulum and Ca super(2+ entry via non store-operated Ca) super(2)+ channels. Thymol induced cell death that may involve apoptosis.</description><identifier>ISSN: 0014-2999</identifier><identifier>DOI: 10.1016/j.ejphar.2011.08.017</identifier><language>eng</language><subject>12-O-Tetradecanoylphorbol-13-acetate ; Annexin V ; Apoptosis ; Ca super(2+)-transporting ATPase ; Calcium ; Calcium (extracellular) ; Calcium channels ; Calcium homeostasis ; Calcium influx ; Endoplasmic reticulum ; Essential oils ; Fura-2 ; glioblastoma cells ; Phorbol esters ; Phospholipase C ; propidium iodide ; Protein kinase C ; thapsigargin ; thymol</subject><ispartof>European journal of pharmacology, 2011-11, Vol.670 (1), p.85-91</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids></links><search><creatorcontrib>Hsu, Shu-Shong</creatorcontrib><creatorcontrib>Lin, Ko-Long</creatorcontrib><creatorcontrib>Chou, Chiang-Ting</creatorcontrib><creatorcontrib>Chiang, An-Jen</creatorcontrib><creatorcontrib>Liang, Wei-Zhe</creatorcontrib><creatorcontrib>Chang, Hong-Tai</creatorcontrib><creatorcontrib>Tsai, Jeng-Yu</creatorcontrib><creatorcontrib>Liao, Wei-Chuan</creatorcontrib><creatorcontrib>Huang, Fong-Dee</creatorcontrib><creatorcontrib>Huang, Jong Khing</creatorcontrib><creatorcontrib>Chen, I-Shu</creatorcontrib><creatorcontrib>Liu, Shuih-Inn</creatorcontrib><creatorcontrib>Kuo, Chun-Chi</creatorcontrib><creatorcontrib>Jan, Chung-Ren</creatorcontrib><title>Effect of thymol on Ca super(2+ homeostasis and viability in human glioblastoma cells)</title><title>European journal of pharmacology</title><description>The effect of the natural essential oil thymol on cytosolic Ca super(2+ concentrations ([Ca) super(2)+] sub(i) and viability in human glioblastoma cells was examined. The Ca) super(2)+-sensitive fluorescent dye fura-2 was applied to measure [Ca super(2+]) sub(i). Thymol at concentrations of 400-1000 mu M induced a [Ca super(2+]) sub(i) rise in a concentration-dependent fashion. The response was decreased partially by removal of extracellular Ca super(2+. Thymol-induced Ca) super(2)+ signal was not altered by nifedipine, econazole, SK&F96365, and protein kinase C activator phorbol myristate acetate (PMA), but was inhibited by the protein kinase C inhibitor GF109203X. When extracellular Ca super(2+ was removed, incubation with the endoplasmic reticulum Ca) super(2)+ pump inhibitor thapsigargin or 2,5-di-tert-butylhydroquinone (BHQ) abolished thymol-induced [Ca super(2+]) sub(i) rise. Incubation with thymol also abolished thapsigargin or BHQ-induced [Ca super(2+]) sub(i) rise. Inhibition of phospholipase C with U73122 abolished thymol-induced [Ca super(2+]) sub(i) rise. At concentrations of 200-800 mu M, thymol killed cells in a concentration-dependent manner. This cytotoxic effect was not changed by chelating cytosolic Ca super(2+ with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl (BAPTA/AM). Annexin V/propidium iodide staining data suggest that thymol (200, 400 and 600 mu M) induced apoptosis in a concentration-dependent manner. Collectively, in human glioblastoma cells, thymol induced a [Ca) super(2)+] sub(i rise by inducing phospholipase C- and protein kinase C-dependent Ca) super(2)+ release from the endoplasmic reticulum and Ca super(2+ entry via non store-operated Ca) super(2)+ channels. Thymol induced cell death that may involve apoptosis.</description><subject>12-O-Tetradecanoylphorbol-13-acetate</subject><subject>Annexin V</subject><subject>Apoptosis</subject><subject>Ca super(2+)-transporting ATPase</subject><subject>Calcium</subject><subject>Calcium (extracellular)</subject><subject>Calcium channels</subject><subject>Calcium homeostasis</subject><subject>Calcium influx</subject><subject>Endoplasmic reticulum</subject><subject>Essential oils</subject><subject>Fura-2</subject><subject>glioblastoma cells</subject><subject>Phorbol esters</subject><subject>Phospholipase C</subject><subject>propidium iodide</subject><subject>Protein kinase C</subject><subject>thapsigargin</subject><subject>thymol</subject><issn>0014-2999</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqVyrFuwjAQAFAPVIIW_oDhRqoKc04I4BlR9QOqrugAhziyfSHnVOLvKyR-oNNbnlJzg9qg2axa7dquoV4XaIzGnUazHakJolkvC2vtWL2KtIhY2aKaqJ9DXbtzBq4hN_fIATjBnkCGzvWL4gMajo4lk3gBShf49XTywec7-ATNECnBNXg-BZLMkeDsQpD3qXqpKYibPX1Ti8_D9_5r2fV8G5zkY_TyqJQcD3I0aMt1WdnKlv-ofzZCSw8</recordid><startdate>20111116</startdate><enddate>20111116</enddate><creator>Hsu, Shu-Shong</creator><creator>Lin, Ko-Long</creator><creator>Chou, Chiang-Ting</creator><creator>Chiang, An-Jen</creator><creator>Liang, Wei-Zhe</creator><creator>Chang, Hong-Tai</creator><creator>Tsai, Jeng-Yu</creator><creator>Liao, Wei-Chuan</creator><creator>Huang, Fong-Dee</creator><creator>Huang, Jong Khing</creator><creator>Chen, I-Shu</creator><creator>Liu, Shuih-Inn</creator><creator>Kuo, Chun-Chi</creator><creator>Jan, Chung-Ren</creator><scope>7TK</scope></search><sort><creationdate>20111116</creationdate><title>Effect of thymol on Ca super(2+ homeostasis and viability in human glioblastoma cells)</title><author>Hsu, Shu-Shong ; Lin, Ko-Long ; Chou, Chiang-Ting ; Chiang, An-Jen ; Liang, Wei-Zhe ; Chang, Hong-Tai ; Tsai, Jeng-Yu ; Liao, Wei-Chuan ; Huang, Fong-Dee ; Huang, Jong Khing ; Chen, I-Shu ; Liu, Shuih-Inn ; Kuo, Chun-Chi ; Jan, Chung-Ren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_10934359593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>12-O-Tetradecanoylphorbol-13-acetate</topic><topic>Annexin V</topic><topic>Apoptosis</topic><topic>Ca super(2+)-transporting ATPase</topic><topic>Calcium</topic><topic>Calcium (extracellular)</topic><topic>Calcium channels</topic><topic>Calcium homeostasis</topic><topic>Calcium influx</topic><topic>Endoplasmic reticulum</topic><topic>Essential oils</topic><topic>Fura-2</topic><topic>glioblastoma cells</topic><topic>Phorbol esters</topic><topic>Phospholipase C</topic><topic>propidium iodide</topic><topic>Protein kinase C</topic><topic>thapsigargin</topic><topic>thymol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsu, Shu-Shong</creatorcontrib><creatorcontrib>Lin, Ko-Long</creatorcontrib><creatorcontrib>Chou, Chiang-Ting</creatorcontrib><creatorcontrib>Chiang, An-Jen</creatorcontrib><creatorcontrib>Liang, Wei-Zhe</creatorcontrib><creatorcontrib>Chang, Hong-Tai</creatorcontrib><creatorcontrib>Tsai, Jeng-Yu</creatorcontrib><creatorcontrib>Liao, Wei-Chuan</creatorcontrib><creatorcontrib>Huang, Fong-Dee</creatorcontrib><creatorcontrib>Huang, Jong Khing</creatorcontrib><creatorcontrib>Chen, I-Shu</creatorcontrib><creatorcontrib>Liu, Shuih-Inn</creatorcontrib><creatorcontrib>Kuo, Chun-Chi</creatorcontrib><creatorcontrib>Jan, Chung-Ren</creatorcontrib><collection>Neurosciences Abstracts</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsu, Shu-Shong</au><au>Lin, Ko-Long</au><au>Chou, Chiang-Ting</au><au>Chiang, An-Jen</au><au>Liang, Wei-Zhe</au><au>Chang, Hong-Tai</au><au>Tsai, Jeng-Yu</au><au>Liao, Wei-Chuan</au><au>Huang, Fong-Dee</au><au>Huang, Jong Khing</au><au>Chen, I-Shu</au><au>Liu, Shuih-Inn</au><au>Kuo, Chun-Chi</au><au>Jan, Chung-Ren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of thymol on Ca super(2+ homeostasis and viability in human glioblastoma cells)</atitle><jtitle>European journal of pharmacology</jtitle><date>2011-11-16</date><risdate>2011</risdate><volume>670</volume><issue>1</issue><spage>85</spage><epage>91</epage><pages>85-91</pages><issn>0014-2999</issn><abstract>The effect of the natural essential oil thymol on cytosolic Ca super(2+ concentrations ([Ca) super(2)+] sub(i) and viability in human glioblastoma cells was examined. The Ca) super(2)+-sensitive fluorescent dye fura-2 was applied to measure [Ca super(2+]) sub(i). Thymol at concentrations of 400-1000 mu M induced a [Ca super(2+]) sub(i) rise in a concentration-dependent fashion. The response was decreased partially by removal of extracellular Ca super(2+. Thymol-induced Ca) super(2)+ signal was not altered by nifedipine, econazole, SK&F96365, and protein kinase C activator phorbol myristate acetate (PMA), but was inhibited by the protein kinase C inhibitor GF109203X. When extracellular Ca super(2+ was removed, incubation with the endoplasmic reticulum Ca) super(2)+ pump inhibitor thapsigargin or 2,5-di-tert-butylhydroquinone (BHQ) abolished thymol-induced [Ca super(2+]) sub(i) rise. Incubation with thymol also abolished thapsigargin or BHQ-induced [Ca super(2+]) sub(i) rise. Inhibition of phospholipase C with U73122 abolished thymol-induced [Ca super(2+]) sub(i) rise. At concentrations of 200-800 mu M, thymol killed cells in a concentration-dependent manner. This cytotoxic effect was not changed by chelating cytosolic Ca super(2+ with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl (BAPTA/AM). Annexin V/propidium iodide staining data suggest that thymol (200, 400 and 600 mu M) induced apoptosis in a concentration-dependent manner. Collectively, in human glioblastoma cells, thymol induced a [Ca) super(2)+] sub(i rise by inducing phospholipase C- and protein kinase C-dependent Ca) super(2)+ release from the endoplasmic reticulum and Ca super(2+ entry via non store-operated Ca) super(2)+ channels. Thymol induced cell death that may involve apoptosis.</abstract><doi>10.1016/j.ejphar.2011.08.017</doi></addata></record> |
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| subjects | 12-O-Tetradecanoylphorbol-13-acetate Annexin V Apoptosis Ca super(2+)-transporting ATPase Calcium Calcium (extracellular) Calcium channels Calcium homeostasis Calcium influx Endoplasmic reticulum Essential oils Fura-2 glioblastoma cells Phorbol esters Phospholipase C propidium iodide Protein kinase C thapsigargin thymol |
| title | Effect of thymol on Ca super(2+ homeostasis and viability in human glioblastoma cells) |
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