Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini‐ and Conventional STR Kits
: Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medi...
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| Veröffentlicht in: | Journal of forensic sciences 2012-05, Vol.57 (3), p.820-825 |
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| container_title | Journal of forensic sciences |
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| creator | Oh, Chang Seok Lee, Sang Jun Park, Jun Bum Lee, Soong Deok Seo, Seung Bum Kim, Hye Yeon Kim, Jaehyup Kim, Yi‐Suk Shin, Dong Hoon |
| description | : Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger‐sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large‐sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples. |
| doi_str_mv | 10.1111/j.1556-4029.2011.02044.x |
| format | Article |
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In this study, we tested newly developed AmpFℓSTR® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger‐sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large‐sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.</description><identifier>ISSN: 0022-1198</identifier><identifier>EISSN: 1556-4029</identifier><identifier>DOI: 10.1111/j.1556-4029.2011.02044.x</identifier><identifier>PMID: 22268568</identifier><identifier>CODEN: JFSCAS</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>AmpFℓSTR® Identifiler™ kit ; AmpFℓSTR® MiniFiler™ kit ; Analytical chemistry ; ancient DNA ; autosomal short tandem repeat genotyping ; Deoxyribonucleic acid ; DNA ; DNA - isolation & purification ; DNA Degradation, Necrotic ; DNA Fingerprinting - instrumentation ; DNA Fingerprinting - methods ; Femur - chemistry ; forensic science ; Forensic sciences ; Gene loci ; Genotype & phenotype ; Genotyping Techniques - instrumentation ; Genotyping Techniques - methods ; History, 16th Century ; History, 18th Century ; Humans ; Korea ; medieval human sample ; Microsatellite Repeats ; Polymerase Chain Reaction</subject><ispartof>Journal of forensic sciences, 2012-05, Vol.57 (3), p.820-825</ispartof><rights>2012 American Academy of Forensic Sciences</rights><rights>2012 American Academy of Forensic Sciences.</rights><rights>Copyright American Society for Testing and Materials May 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4964-3087a92337f65c829b084be8391b9592323e47986176f8d9e0ef706dcf6d3f3f3</citedby><cites>FETCH-LOGICAL-c4964-3087a92337f65c829b084be8391b9592323e47986176f8d9e0ef706dcf6d3f3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1556-4029.2011.02044.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1556-4029.2011.02044.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22268568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oh, Chang Seok</creatorcontrib><creatorcontrib>Lee, Sang Jun</creatorcontrib><creatorcontrib>Park, Jun Bum</creatorcontrib><creatorcontrib>Lee, Soong Deok</creatorcontrib><creatorcontrib>Seo, Seung Bum</creatorcontrib><creatorcontrib>Kim, Hye Yeon</creatorcontrib><creatorcontrib>Kim, Jaehyup</creatorcontrib><creatorcontrib>Kim, Yi‐Suk</creatorcontrib><creatorcontrib>Shin, Dong Hoon</creatorcontrib><title>Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini‐ and Conventional STR Kits</title><title>Journal of forensic sciences</title><addtitle>J Forensic Sci</addtitle><description>: Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger‐sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large‐sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.</description><subject>AmpFℓSTR® Identifiler™ kit</subject><subject>AmpFℓSTR® MiniFiler™ kit</subject><subject>Analytical chemistry</subject><subject>ancient DNA</subject><subject>autosomal short tandem repeat genotyping</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - isolation & purification</subject><subject>DNA Degradation, Necrotic</subject><subject>DNA Fingerprinting - instrumentation</subject><subject>DNA Fingerprinting - methods</subject><subject>Femur - chemistry</subject><subject>forensic science</subject><subject>Forensic sciences</subject><subject>Gene loci</subject><subject>Genotype & phenotype</subject><subject>Genotyping Techniques - instrumentation</subject><subject>Genotyping Techniques - methods</subject><subject>History, 16th Century</subject><subject>History, 18th Century</subject><subject>Humans</subject><subject>Korea</subject><subject>medieval human sample</subject><subject>Microsatellite Repeats</subject><subject>Polymerase Chain Reaction</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUha2qiA6FV6gsddNNwvVPHHvBYjRQChQqlenays-N8CiJp3ECnR2PwDP2SXA6pQtWtRe-0vnOubIOIZRByuJ5u0lZlqlEAjcpB8ZS4CBlendAFk_CIVkAcJ4wZvQReRXCBgAUU-wlOeKcK50pvSBuOY0--K5o6fcffhjpuuhr7Og1brEY6bIv2l1wgfomzpXDfqTvvy1puaMrP21brOlNwFn96np3__sPjfYo9T8j6Xw_x66v6Rc3htfkRVO0Ad88vsfk5vzDenWRXF59_LRaXiaVNEomAnReGC5E3qis0tyUoGWJWhhWmiwKXKDMjVYsV42uDQI2Oai6alQtmniPydk-dzv42wnDaDsXKmzbokc_BcvACCmEYPAMlCmVK5BZRE__Qzd-GuL_HijgmRbZHKj3VDX4EAZs7HZwXTHsImTn5uzGzgXZuSA7N2cfmrN30XryuGAqO6yfjP-qisC7PfDLtbh7drD9fH41T-Iv_JWkMA</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Oh, Chang Seok</creator><creator>Lee, Sang Jun</creator><creator>Park, Jun Bum</creator><creator>Lee, Soong Deok</creator><creator>Seo, Seung Bum</creator><creator>Kim, Hye Yeon</creator><creator>Kim, Jaehyup</creator><creator>Kim, Yi‐Suk</creator><creator>Shin, Dong Hoon</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>201205</creationdate><title>Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini‐ and Conventional STR Kits</title><author>Oh, Chang Seok ; Lee, Sang Jun ; Park, Jun Bum ; Lee, Soong Deok ; Seo, Seung Bum ; Kim, Hye Yeon ; Kim, Jaehyup ; Kim, Yi‐Suk ; Shin, Dong Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4964-3087a92337f65c829b084be8391b9592323e47986176f8d9e0ef706dcf6d3f3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>AmpFℓSTR® Identifiler™ kit</topic><topic>AmpFℓSTR® MiniFiler™ kit</topic><topic>Analytical chemistry</topic><topic>ancient DNA</topic><topic>autosomal short tandem repeat genotyping</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>DNA Degradation, Necrotic</topic><topic>DNA Fingerprinting - instrumentation</topic><topic>DNA Fingerprinting - methods</topic><topic>Femur - chemistry</topic><topic>forensic science</topic><topic>Forensic sciences</topic><topic>Gene loci</topic><topic>Genotype & phenotype</topic><topic>Genotyping Techniques - instrumentation</topic><topic>Genotyping Techniques - methods</topic><topic>History, 16th Century</topic><topic>History, 18th Century</topic><topic>Humans</topic><topic>Korea</topic><topic>medieval human sample</topic><topic>Microsatellite Repeats</topic><topic>Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oh, Chang Seok</creatorcontrib><creatorcontrib>Lee, Sang Jun</creatorcontrib><creatorcontrib>Park, Jun Bum</creatorcontrib><creatorcontrib>Lee, Soong Deok</creatorcontrib><creatorcontrib>Seo, Seung Bum</creatorcontrib><creatorcontrib>Kim, Hye Yeon</creatorcontrib><creatorcontrib>Kim, Jaehyup</creatorcontrib><creatorcontrib>Kim, Yi‐Suk</creatorcontrib><creatorcontrib>Shin, Dong Hoon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oh, Chang Seok</au><au>Lee, Sang Jun</au><au>Park, Jun Bum</au><au>Lee, Soong Deok</au><au>Seo, Seung Bum</au><au>Kim, Hye Yeon</au><au>Kim, Jaehyup</au><au>Kim, Yi‐Suk</au><au>Shin, Dong Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini‐ and Conventional STR Kits</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2012-05</date><risdate>2012</risdate><volume>57</volume><issue>3</issue><spage>820</spage><epage>825</epage><pages>820-825</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><coden>JFSCAS</coden><abstract>: Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger‐sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large‐sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22268568</pmid><doi>10.1111/j.1556-4029.2011.02044.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of forensic sciences, 2012-05, Vol.57 (3), p.820-825 |
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| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | AmpFℓSTR® Identifiler™ kit AmpFℓSTR® MiniFiler™ kit Analytical chemistry ancient DNA autosomal short tandem repeat genotyping Deoxyribonucleic acid DNA DNA - isolation & purification DNA Degradation, Necrotic DNA Fingerprinting - instrumentation DNA Fingerprinting - methods Femur - chemistry forensic science Forensic sciences Gene loci Genotype & phenotype Genotyping Techniques - instrumentation Genotyping Techniques - methods History, 16th Century History, 18th Century Humans Korea medieval human sample Microsatellite Repeats Polymerase Chain Reaction |
| title | Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini‐ and Conventional STR Kits |
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