Antiphotoaging Effects of Light‐Emitting Diode Irradiation on Narrow‐Band Ultraviolet B–Exposed Cultured Human Skin Cells
Background Antiaging effects of light‐emitting diodes (LEDs) have been clinically demonstrated using one or two wavelengths. The mechanism is unclear. Objective To examine direct and indirect photobiomodulation effects of LEDs on narrow‐band ultraviolet B (NB‐UVB)‐induced photoaging using seven diff...
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| Veröffentlicht in: | Dermatologic surgery 2012-10, Vol.38 (10), p.1695-1703 |
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| container_title | Dermatologic surgery |
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| creator | Tian, Yu Shun Kim, Nan‐Hyung Lee, Ai‐Young |
| description | Background
Antiaging effects of light‐emitting diodes (LEDs) have been clinically demonstrated using one or two wavelengths. The mechanism is unclear.
Objective
To examine direct and indirect photobiomodulation effects of LEDs on narrow‐band ultraviolet B (NB‐UVB)‐induced photoaging using seven different wavelengths alone or in combination.
Materials and Methods
Four LED wavelengths were chosen based on type I collagen and metalloproteinase (MMP)‐1 expression. NB‐UVB‐irradiated fibroblasts or keratinocytes were irradiated using these four wavelengths. The expression of collagen and MMP‐1 in fibroblasts with or without conditioned medium from LED‐irradiated keratinocytes and the expression of proinflammatory cytokines in the LED‐irradiated keratinocytes were examined.
Results
Irradiation with four wavelengths (630, 660, 830, and 850 nm) significantly increased the number of viable fibroblasts. These four wavelengths also increased type I collagen expression, particularly four combinations (630/830, 660/850, 630/850, and 660/830 nm). The fibroblasts cultured with the keratinocyte conditioned medium, particularly with a combination of 630/850 or 660/830 nm, increased collagen levels. Low tumor necrosis factor alpha (TNF‐α) and high transforming growth factor beta 1 (TGF‐β1) expression was detected in the LED‐irradiated keratinocytes.
Conclusion
The combination of 630/850‐ or 660/830‐nm irradiation led to better direct and indirect antiphotoaging outcomes than single LED wavelengths in NB‐UVB‐irradiated cultured normal human skin cells. |
| doi_str_mv | 10.1111/j.1524-4725.2012.02501.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1082407284</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1082407284</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3991-2e0bba8688b4aabedfba94ef59ee614b6ace5c00582a296dbb147c4a133061fe3</originalsourceid><addsrcrecordid>eNqNkMFu1DAQhi0EoqXwCsgXJC4JtuNkkwOHdru0lVZwKHu2Js5k6yWJF9uh2xN9hEq8YZ8Eh13KFcuSf2m-GY8-QihnKY_nwybluZCJnIk8FYyLlImc8XT3jBw_FZ7HzGZFwnIujsgr7zcsklXGXpIjIUomy6I6Jj9Ph2C2NzZYWJthTRdtizp4alu6NOub8Hj_sOhNCFPt3NgG6ZVz0BgIxg403s_gnL2N2BkMDV11wcEPYzsM9Ozx_tdit7UeGzofuzC6GC7HHgZ6_c0MdI5d51-TFy10Ht8c3hOy-rT4Or9Mll8uruany0RnVcUTgayuoSzKspYANTZtDZXENq8QCy7rAjTmmrG8FCCqoqlrLmdaAs8yVvAWsxPyfj936-z3EX1QvfE6bgAD2tErzkoh2UyUMqLlHtXOeu-wVVtnenB3EVKTfrVRk2U1WVaTfvVHv9rF1reHX8a6x-ap8a_vCLw7AOA1dK2DQRv_jytynldZFrmPe-7WdHj33wuo8-vVlLLfgxSlFg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1082407284</pqid></control><display><type>article</type><title>Antiphotoaging Effects of Light‐Emitting Diode Irradiation on Narrow‐Band Ultraviolet B–Exposed Cultured Human Skin Cells</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Journals@Ovid Complete</source><creator>Tian, Yu Shun ; Kim, Nan‐Hyung ; Lee, Ai‐Young</creator><creatorcontrib>Tian, Yu Shun ; Kim, Nan‐Hyung ; Lee, Ai‐Young</creatorcontrib><description>Background
Antiaging effects of light‐emitting diodes (LEDs) have been clinically demonstrated using one or two wavelengths. The mechanism is unclear.
Objective
To examine direct and indirect photobiomodulation effects of LEDs on narrow‐band ultraviolet B (NB‐UVB)‐induced photoaging using seven different wavelengths alone or in combination.
Materials and Methods
Four LED wavelengths were chosen based on type I collagen and metalloproteinase (MMP)‐1 expression. NB‐UVB‐irradiated fibroblasts or keratinocytes were irradiated using these four wavelengths. The expression of collagen and MMP‐1 in fibroblasts with or without conditioned medium from LED‐irradiated keratinocytes and the expression of proinflammatory cytokines in the LED‐irradiated keratinocytes were examined.
Results
Irradiation with four wavelengths (630, 660, 830, and 850 nm) significantly increased the number of viable fibroblasts. These four wavelengths also increased type I collagen expression, particularly four combinations (630/830, 660/850, 630/850, and 660/830 nm). The fibroblasts cultured with the keratinocyte conditioned medium, particularly with a combination of 630/850 or 660/830 nm, increased collagen levels. Low tumor necrosis factor alpha (TNF‐α) and high transforming growth factor beta 1 (TGF‐β1) expression was detected in the LED‐irradiated keratinocytes.
Conclusion
The combination of 630/850‐ or 660/830‐nm irradiation led to better direct and indirect antiphotoaging outcomes than single LED wavelengths in NB‐UVB‐irradiated cultured normal human skin cells.</description><identifier>ISSN: 1076-0512</identifier><identifier>EISSN: 1524-4725</identifier><identifier>DOI: 10.1111/j.1524-4725.2012.02501.x</identifier><identifier>PMID: 22804869</identifier><language>eng</language><publisher>Malden, MA: Wiley</publisher><subject>Analysis of Variance ; Biological and medical sciences ; Cell Proliferation - radiation effects ; Collagen Type I - biosynthesis ; Dermatology ; Fibroblasts - metabolism ; Fibroblasts - radiation effects ; Humans ; Keratinocytes - metabolism ; Keratinocytes - radiation effects ; Matrix Metalloproteinase 1 - metabolism ; Medical sciences ; Phototherapy - methods ; Skin Aging ; Skin plastic surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Transforming Growth Factor beta1 - metabolism ; Tumor Necrosis Factor-alpha - metabolism ; Ultraviolet Rays - adverse effects</subject><ispartof>Dermatologic surgery, 2012-10, Vol.38 (10), p.1695-1703</ispartof><rights>2012 by the American Society for Dermatologic Surgery, Inc. Published by Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3991-2e0bba8688b4aabedfba94ef59ee614b6ace5c00582a296dbb147c4a133061fe3</citedby><cites>FETCH-LOGICAL-c3991-2e0bba8688b4aabedfba94ef59ee614b6ace5c00582a296dbb147c4a133061fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1524-4725.2012.02501.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1524-4725.2012.02501.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26515933$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22804869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Yu Shun</creatorcontrib><creatorcontrib>Kim, Nan‐Hyung</creatorcontrib><creatorcontrib>Lee, Ai‐Young</creatorcontrib><title>Antiphotoaging Effects of Light‐Emitting Diode Irradiation on Narrow‐Band Ultraviolet B–Exposed Cultured Human Skin Cells</title><title>Dermatologic surgery</title><addtitle>Dermatol Surg</addtitle><description>Background
Antiaging effects of light‐emitting diodes (LEDs) have been clinically demonstrated using one or two wavelengths. The mechanism is unclear.
Objective
To examine direct and indirect photobiomodulation effects of LEDs on narrow‐band ultraviolet B (NB‐UVB)‐induced photoaging using seven different wavelengths alone or in combination.
Materials and Methods
Four LED wavelengths were chosen based on type I collagen and metalloproteinase (MMP)‐1 expression. NB‐UVB‐irradiated fibroblasts or keratinocytes were irradiated using these four wavelengths. The expression of collagen and MMP‐1 in fibroblasts with or without conditioned medium from LED‐irradiated keratinocytes and the expression of proinflammatory cytokines in the LED‐irradiated keratinocytes were examined.
Results
Irradiation with four wavelengths (630, 660, 830, and 850 nm) significantly increased the number of viable fibroblasts. These four wavelengths also increased type I collagen expression, particularly four combinations (630/830, 660/850, 630/850, and 660/830 nm). The fibroblasts cultured with the keratinocyte conditioned medium, particularly with a combination of 630/850 or 660/830 nm, increased collagen levels. Low tumor necrosis factor alpha (TNF‐α) and high transforming growth factor beta 1 (TGF‐β1) expression was detected in the LED‐irradiated keratinocytes.
Conclusion
The combination of 630/850‐ or 660/830‐nm irradiation led to better direct and indirect antiphotoaging outcomes than single LED wavelengths in NB‐UVB‐irradiated cultured normal human skin cells.</description><subject>Analysis of Variance</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation - radiation effects</subject><subject>Collagen Type I - biosynthesis</subject><subject>Dermatology</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - radiation effects</subject><subject>Humans</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - radiation effects</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Medical sciences</subject><subject>Phototherapy - methods</subject><subject>Skin Aging</subject><subject>Skin plastic surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Ultraviolet Rays - adverse effects</subject><issn>1076-0512</issn><issn>1524-4725</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFu1DAQhi0EoqXwCsgXJC4JtuNkkwOHdru0lVZwKHu2Js5k6yWJF9uh2xN9hEq8YZ8Eh13KFcuSf2m-GY8-QihnKY_nwybluZCJnIk8FYyLlImc8XT3jBw_FZ7HzGZFwnIujsgr7zcsklXGXpIjIUomy6I6Jj9Ph2C2NzZYWJthTRdtizp4alu6NOub8Hj_sOhNCFPt3NgG6ZVz0BgIxg403s_gnL2N2BkMDV11wcEPYzsM9Ozx_tdit7UeGzofuzC6GC7HHgZ6_c0MdI5d51-TFy10Ht8c3hOy-rT4Or9Mll8uruany0RnVcUTgayuoSzKspYANTZtDZXENq8QCy7rAjTmmrG8FCCqoqlrLmdaAs8yVvAWsxPyfj936-z3EX1QvfE6bgAD2tErzkoh2UyUMqLlHtXOeu-wVVtnenB3EVKTfrVRk2U1WVaTfvVHv9rF1reHX8a6x-ap8a_vCLw7AOA1dK2DQRv_jytynldZFrmPe-7WdHj33wuo8-vVlLLfgxSlFg</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>Tian, Yu Shun</creator><creator>Kim, Nan‐Hyung</creator><creator>Lee, Ai‐Young</creator><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201210</creationdate><title>Antiphotoaging Effects of Light‐Emitting Diode Irradiation on Narrow‐Band Ultraviolet B–Exposed Cultured Human Skin Cells</title><author>Tian, Yu Shun ; Kim, Nan‐Hyung ; Lee, Ai‐Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3991-2e0bba8688b4aabedfba94ef59ee614b6ace5c00582a296dbb147c4a133061fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis of Variance</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation - radiation effects</topic><topic>Collagen Type I - biosynthesis</topic><topic>Dermatology</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - radiation effects</topic><topic>Humans</topic><topic>Keratinocytes - metabolism</topic><topic>Keratinocytes - radiation effects</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Medical sciences</topic><topic>Phototherapy - methods</topic><topic>Skin Aging</topic><topic>Skin plastic surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Ultraviolet Rays - adverse effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Yu Shun</creatorcontrib><creatorcontrib>Kim, Nan‐Hyung</creatorcontrib><creatorcontrib>Lee, Ai‐Young</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Dermatologic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Yu Shun</au><au>Kim, Nan‐Hyung</au><au>Lee, Ai‐Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiphotoaging Effects of Light‐Emitting Diode Irradiation on Narrow‐Band Ultraviolet B–Exposed Cultured Human Skin Cells</atitle><jtitle>Dermatologic surgery</jtitle><addtitle>Dermatol Surg</addtitle><date>2012-10</date><risdate>2012</risdate><volume>38</volume><issue>10</issue><spage>1695</spage><epage>1703</epage><pages>1695-1703</pages><issn>1076-0512</issn><eissn>1524-4725</eissn><abstract>Background
Antiaging effects of light‐emitting diodes (LEDs) have been clinically demonstrated using one or two wavelengths. The mechanism is unclear.
Objective
To examine direct and indirect photobiomodulation effects of LEDs on narrow‐band ultraviolet B (NB‐UVB)‐induced photoaging using seven different wavelengths alone or in combination.
Materials and Methods
Four LED wavelengths were chosen based on type I collagen and metalloproteinase (MMP)‐1 expression. NB‐UVB‐irradiated fibroblasts or keratinocytes were irradiated using these four wavelengths. The expression of collagen and MMP‐1 in fibroblasts with or without conditioned medium from LED‐irradiated keratinocytes and the expression of proinflammatory cytokines in the LED‐irradiated keratinocytes were examined.
Results
Irradiation with four wavelengths (630, 660, 830, and 850 nm) significantly increased the number of viable fibroblasts. These four wavelengths also increased type I collagen expression, particularly four combinations (630/830, 660/850, 630/850, and 660/830 nm). The fibroblasts cultured with the keratinocyte conditioned medium, particularly with a combination of 630/850 or 660/830 nm, increased collagen levels. Low tumor necrosis factor alpha (TNF‐α) and high transforming growth factor beta 1 (TGF‐β1) expression was detected in the LED‐irradiated keratinocytes.
Conclusion
The combination of 630/850‐ or 660/830‐nm irradiation led to better direct and indirect antiphotoaging outcomes than single LED wavelengths in NB‐UVB‐irradiated cultured normal human skin cells.</abstract><cop>Malden, MA</cop><pub>Wiley</pub><pmid>22804869</pmid><doi>10.1111/j.1524-4725.2012.02501.x</doi><tpages>9</tpages></addata></record> |
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| ispartof | Dermatologic surgery, 2012-10, Vol.38 (10), p.1695-1703 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Journals@Ovid Complete |
| subjects | Analysis of Variance Biological and medical sciences Cell Proliferation - radiation effects Collagen Type I - biosynthesis Dermatology Fibroblasts - metabolism Fibroblasts - radiation effects Humans Keratinocytes - metabolism Keratinocytes - radiation effects Matrix Metalloproteinase 1 - metabolism Medical sciences Phototherapy - methods Skin Aging Skin plastic surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Transforming Growth Factor beta1 - metabolism Tumor Necrosis Factor-alpha - metabolism Ultraviolet Rays - adverse effects |
| title | Antiphotoaging Effects of Light‐Emitting Diode Irradiation on Narrow‐Band Ultraviolet B–Exposed Cultured Human Skin Cells |
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