Reusable Solid-Phase Microextraction Coating for Direct Immersion Whole-Blood Analysis and Extracted Blood Spot Sampling Coupled with Liquid Chromatography–Tandem Mass Spectrometry and Direct Analysis in Real Time–Tandem Mass Spectrometry
Three different biocompatible polymers were tested and evaluated in order to improve the whole-blood biocompatibility of previously developed C18–polyacrylonitrile (C18–PAN) thin-film solid-phase microextraction (SPME) coating. Among all methods of modification, UV-dried thin PAN-over C18–PAN provid...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2012-10, Vol.84 (19), p.8301-8309 |
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| creator | Mirnaghi, Fatemeh S Pawliszyn, Janusz |
| description | Three different biocompatible polymers were tested and evaluated in order to improve the whole-blood biocompatibility of previously developed C18–polyacrylonitrile (C18–PAN) thin-film solid-phase microextraction (SPME) coating. Among all methods of modification, UV-dried thin PAN-over C18–PAN provided the best results. This coating presented reusable properties and reproducible extraction efficiency for at least 30 direct extractions of diazepam from whole blood [relative standard deviation (RSD) = 12% using external calibration and 4% using isotope dilution calibration]. The amount of absolute recovery for direct immersion analysis and based on the free concentration of diazepam in blood matrix was about 4.8% (desorption efficiency = 98%). The limit of quantitation (LOQ) for the developed solid-phase microextraction liquid chromatography–tandem mass spectrometry (SPME-LC–MS/MS) method for direct whole-blood analysis was 0.5 ng/mL. The optimized modification of the coating was then used for an extracted blood spot (EBS) sampling approach, a new sampling method which is introduced to address the limitations of dried blood spot sampling. EBS was evaluated using LC–MS/MS and direct analysis in real time (DART)–MS/MS, where, for a 5 μL blood spot, LOQs of 0.2 and 1 μg/mL, respectively, were achieved for extraction of diazepam. |
| doi_str_mv | 10.1021/ac3018229 |
| format | Article |
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Among all methods of modification, UV-dried thin PAN-over C18–PAN provided the best results. This coating presented reusable properties and reproducible extraction efficiency for at least 30 direct extractions of diazepam from whole blood [relative standard deviation (RSD) = 12% using external calibration and 4% using isotope dilution calibration]. The amount of absolute recovery for direct immersion analysis and based on the free concentration of diazepam in blood matrix was about 4.8% (desorption efficiency = 98%). The limit of quantitation (LOQ) for the developed solid-phase microextraction liquid chromatography–tandem mass spectrometry (SPME-LC–MS/MS) method for direct whole-blood analysis was 0.5 ng/mL. The optimized modification of the coating was then used for an extracted blood spot (EBS) sampling approach, a new sampling method which is introduced to address the limitations of dried blood spot sampling. EBS was evaluated using LC–MS/MS and direct analysis in real time (DART)–MS/MS, where, for a 5 μL blood spot, LOQs of 0.2 and 1 μg/mL, respectively, were achieved for extraction of diazepam.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac3018229</identifier><identifier>PMID: 22928515</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Biocompatible Materials - chemistry ; Biopolymers ; Blood Chemical Analysis - instrumentation ; Blood tests ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography ; Chromatography, Liquid - instrumentation ; Diazepam - analogs & derivatives ; Diazepam - blood ; Exact sciences and technology ; Humans ; Mass spectrometry ; Other chromatographic methods ; Sensitivity and Specificity ; Solid Phase Microextraction - instrumentation ; Spectrometric and optical methods ; Tandem Mass Spectrometry - instrumentation ; Thin film coatings ; Time Factors</subject><ispartof>Analytical chemistry (Washington), 2012-10, Vol.84 (19), p.8301-8309</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Oct 2, 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a373t-de91c83471f48a88ed4e3bbe59d0ccd43dd25d09d980e1c8b9abb5df89fe15813</citedby><cites>FETCH-LOGICAL-a373t-de91c83471f48a88ed4e3bbe59d0ccd43dd25d09d980e1c8b9abb5df89fe15813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac3018229$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac3018229$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26437554$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22928515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mirnaghi, Fatemeh S</creatorcontrib><creatorcontrib>Pawliszyn, Janusz</creatorcontrib><title>Reusable Solid-Phase Microextraction Coating for Direct Immersion Whole-Blood Analysis and Extracted Blood Spot Sampling Coupled with Liquid Chromatography–Tandem Mass Spectrometry and Direct Analysis in Real Time–Tandem Mass Spectrometry</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Three different biocompatible polymers were tested and evaluated in order to improve the whole-blood biocompatibility of previously developed C18–polyacrylonitrile (C18–PAN) thin-film solid-phase microextraction (SPME) coating. Among all methods of modification, UV-dried thin PAN-over C18–PAN provided the best results. This coating presented reusable properties and reproducible extraction efficiency for at least 30 direct extractions of diazepam from whole blood [relative standard deviation (RSD) = 12% using external calibration and 4% using isotope dilution calibration]. The amount of absolute recovery for direct immersion analysis and based on the free concentration of diazepam in blood matrix was about 4.8% (desorption efficiency = 98%). The limit of quantitation (LOQ) for the developed solid-phase microextraction liquid chromatography–tandem mass spectrometry (SPME-LC–MS/MS) method for direct whole-blood analysis was 0.5 ng/mL. The optimized modification of the coating was then used for an extracted blood spot (EBS) sampling approach, a new sampling method which is introduced to address the limitations of dried blood spot sampling. EBS was evaluated using LC–MS/MS and direct analysis in real time (DART)–MS/MS, where, for a 5 μL blood spot, LOQs of 0.2 and 1 μg/mL, respectively, were achieved for extraction of diazepam.</description><subject>Analytical chemistry</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biopolymers</subject><subject>Blood Chemical Analysis - instrumentation</subject><subject>Blood tests</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography</subject><subject>Chromatography, Liquid - instrumentation</subject><subject>Diazepam - analogs & derivatives</subject><subject>Diazepam - blood</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Mass spectrometry</subject><subject>Other chromatographic methods</subject><subject>Sensitivity and Specificity</subject><subject>Solid Phase Microextraction - instrumentation</subject><subject>Spectrometric and optical methods</subject><subject>Tandem Mass Spectrometry - instrumentation</subject><subject>Thin film coatings</subject><subject>Time Factors</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ksFu1DAURSMEokNhwQ8gSwgJFgE7jmecZRsKVJoK1BnEMnqxXzqunDi1E8Hs-Af-kE_gC_CQYYq6YOXFPe_eZ92XJE8Zfc1oxt6A4pTJLCvuJTMmMprOpczuJzNKKU-zBaVHyaMQrilljLL5w-QoopkUTMySX5c4BqgtkpWzRqefNhCQXBjlHX4bPKjBuI6UDgbTXZHGefLWeFQDOW9b9GEnftk4i-mpdU6Tkw7sNphAoNPkbDJATSZx1buBrKDt7c6rdGNvo_bVDBuyNDej0aTceNfC4K489Jvtz-8_1tEHW3IBIcTxmBt1HPz2j_9-k0Om6cglgiVr0-J_Zh8nDxqwAZ_s3-Pk87uzdfkhXX58f16eLFPgCz6kGgumJM8XrMklSIk6R17XKApNldI51zoTmha6kBQjWRdQ10I3smiQCcn4cfJy8u29uxkxDFVrgkJroUM3horRWBkXjO3Q53fQazf6-K-J4vNYqIjUq4mK5YTgsal6b1rw2whVu0OoDocQ2Wd7x7FuUR_Iv81H4MUegKDANh46ZcItN8_5Qoj8lgMV_t3qbuBvjn7Ncg</recordid><startdate>20121002</startdate><enddate>20121002</enddate><creator>Mirnaghi, Fatemeh S</creator><creator>Pawliszyn, Janusz</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20121002</creationdate><title>Reusable Solid-Phase Microextraction Coating for Direct Immersion Whole-Blood Analysis and Extracted Blood Spot Sampling Coupled with Liquid Chromatography–Tandem Mass Spectrometry and Direct Analysis in Real Time–Tandem Mass Spectrometry</title><author>Mirnaghi, Fatemeh S ; Pawliszyn, Janusz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a373t-de91c83471f48a88ed4e3bbe59d0ccd43dd25d09d980e1c8b9abb5df89fe15813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical chemistry</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biopolymers</topic><topic>Blood Chemical Analysis - instrumentation</topic><topic>Blood tests</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography</topic><topic>Chromatography, Liquid - instrumentation</topic><topic>Diazepam - analogs & derivatives</topic><topic>Diazepam - blood</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Mass spectrometry</topic><topic>Other chromatographic methods</topic><topic>Sensitivity and Specificity</topic><topic>Solid Phase Microextraction - instrumentation</topic><topic>Spectrometric and optical methods</topic><topic>Tandem Mass Spectrometry - instrumentation</topic><topic>Thin film coatings</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mirnaghi, Fatemeh S</creatorcontrib><creatorcontrib>Pawliszyn, Janusz</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mirnaghi, Fatemeh S</au><au>Pawliszyn, Janusz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reusable Solid-Phase Microextraction Coating for Direct Immersion Whole-Blood Analysis and Extracted Blood Spot Sampling Coupled with Liquid Chromatography–Tandem Mass Spectrometry and Direct Analysis in Real Time–Tandem Mass Spectrometry</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2012-10-02</date><risdate>2012</risdate><volume>84</volume><issue>19</issue><spage>8301</spage><epage>8309</epage><pages>8301-8309</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Three different biocompatible polymers were tested and evaluated in order to improve the whole-blood biocompatibility of previously developed C18–polyacrylonitrile (C18–PAN) thin-film solid-phase microextraction (SPME) coating. Among all methods of modification, UV-dried thin PAN-over C18–PAN provided the best results. This coating presented reusable properties and reproducible extraction efficiency for at least 30 direct extractions of diazepam from whole blood [relative standard deviation (RSD) = 12% using external calibration and 4% using isotope dilution calibration]. The amount of absolute recovery for direct immersion analysis and based on the free concentration of diazepam in blood matrix was about 4.8% (desorption efficiency = 98%). The limit of quantitation (LOQ) for the developed solid-phase microextraction liquid chromatography–tandem mass spectrometry (SPME-LC–MS/MS) method for direct whole-blood analysis was 0.5 ng/mL. The optimized modification of the coating was then used for an extracted blood spot (EBS) sampling approach, a new sampling method which is introduced to address the limitations of dried blood spot sampling. EBS was evaluated using LC–MS/MS and direct analysis in real time (DART)–MS/MS, where, for a 5 μL blood spot, LOQs of 0.2 and 1 μg/mL, respectively, were achieved for extraction of diazepam.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>22928515</pmid><doi>10.1021/ac3018229</doi><tpages>9</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2012-10, Vol.84 (19), p.8301-8309 |
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| subjects | Analytical chemistry Biocompatible Materials - chemistry Biopolymers Blood Chemical Analysis - instrumentation Blood tests Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, Liquid - instrumentation Diazepam - analogs & derivatives Diazepam - blood Exact sciences and technology Humans Mass spectrometry Other chromatographic methods Sensitivity and Specificity Solid Phase Microextraction - instrumentation Spectrometric and optical methods Tandem Mass Spectrometry - instrumentation Thin film coatings Time Factors |
| title | Reusable Solid-Phase Microextraction Coating for Direct Immersion Whole-Blood Analysis and Extracted Blood Spot Sampling Coupled with Liquid Chromatography–Tandem Mass Spectrometry and Direct Analysis in Real Time–Tandem Mass Spectrometry |
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