Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC

A better understanding of protein adsorption onto surfaces of materials is required to control biocompatibility and bioactivity. Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of a-C:H and silicon-doped a-C:H thin films (Si-DLC) were deposited onto silicon sub...

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Veröffentlicht in:Journal of materials science 2012-02, Vol.47 (4), p.1729-1736
Hauptverfasser: Ahmed, M., Byrne, A. J., McLaughlin, J., Elhissi, A., Phoenix, D. A., Ahmed, W.
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container_end_page 1736
container_issue 4
container_start_page 1729
container_title Journal of materials science
container_volume 47
creator Ahmed, M.
Byrne, A. J.
McLaughlin, J.
Elhissi, A.
Phoenix, D. A.
Ahmed, W.
description A better understanding of protein adsorption onto surfaces of materials is required to control biocompatibility and bioactivity. Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of a-C:H and silicon-doped a-C:H thin films (Si-DLC) were deposited onto silicon substrates using plasma-enhanced chemical vapour deposition (PECVD). Subsequently, the adsorption of the simplest amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Raman spectroscopy and atomic force microscopy (AFM). The Raman study highlighted a slight decrease in the I D / I G ratio with increasing the silicon dopant levels. Following exposure to glycine solutions, the presence of bands at ~1735 and ~1200 cm −1 indicates that the adsorption of glycine onto the surfaces has taken place. Glycine was bound to the surfaces via both deprotonated carboxyl and protonated amino groups whilst, as the silicon content in the DLC film increased the adsorption of glycine decreased. AFM analysis showed that the surface roughness increased following exposure to glycine. These results show that at low silicon doping the adsorption of the amino acid was enhanced whilst increased doping levels led to a reduced adsorption compared to undoped DLC. Therefore, doping of DLC may provide an approach to control the protein adsorption.
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The Raman study highlighted a slight decrease in the I D / I G ratio with increasing the silicon dopant levels. Following exposure to glycine solutions, the presence of bands at ~1735 and ~1200 cm −1 indicates that the adsorption of glycine onto the surfaces has taken place. Glycine was bound to the surfaces via both deprotonated carboxyl and protonated amino groups whilst, as the silicon content in the DLC film increased the adsorption of glycine decreased. AFM analysis showed that the surface roughness increased following exposure to glycine. These results show that at low silicon doping the adsorption of the amino acid was enhanced whilst increased doping levels led to a reduced adsorption compared to undoped DLC. 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A.</creatorcontrib><creatorcontrib>Ahmed, W.</creatorcontrib><title>Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC</title><title>Journal of materials science</title><addtitle>J Mater Sci</addtitle><description>A better understanding of protein adsorption onto surfaces of materials is required to control biocompatibility and bioactivity. Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of a-C:H and silicon-doped a-C:H thin films (Si-DLC) were deposited onto silicon substrates using plasma-enhanced chemical vapour deposition (PECVD). Subsequently, the adsorption of the simplest amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Raman spectroscopy and atomic force microscopy (AFM). 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Subsequently, the adsorption of the simplest amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Raman spectroscopy and atomic force microscopy (AFM). The Raman study highlighted a slight decrease in the I D / I G ratio with increasing the silicon dopant levels. Following exposure to glycine solutions, the presence of bands at ~1735 and ~1200 cm −1 indicates that the adsorption of glycine onto the surfaces has taken place. Glycine was bound to the surfaces via both deprotonated carboxyl and protonated amino groups whilst, as the silicon content in the DLC film increased the adsorption of glycine decreased. AFM analysis showed that the surface roughness increased following exposure to glycine. 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subjects Adsorption
Amino acids
Analysis
Atomic beam spectroscopy
Atomic force microscopy
Biocompatibility
Characterization and Evaluation of Materials
Chemical properties
Chemical vapor deposition
Chemistry and Materials Science
Classical Mechanics
Crystallography and Scattering Methods
Diamond-like carbon films
Dielectric films
Doping
Glycine
Materials Science
Organic chemistry
Plasma enhanced chemical vapor deposition
Polymer Sciences
Protein adsorption
Proteins
Raman spectroscopy
Silicon
Silicon substrates
Solid Mechanics
Surface chemistry
Surface roughness
Thin films
title Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC
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