Quantitative proteomic analysis of pregnancy-related proteins from peripheral blood mononuclear cells during pregnancy in pigs
Information obtained from peripheral blood could help us understand the underlying mechanisms in autoimmune diseases, cancer, pregnancy, and other conditions. In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression...
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| Veröffentlicht in: | Animal reproduction science 2012-10, Vol.134 (3-4), p.164-176 |
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| creator | Chae, Jung-Il Kim, Jumi Lee, Seong Goo Koh, Min Whan Jeon, Young-Joo Kim, Dong-Wook Ko, Seon mi Seo, Kang Seok Lee, Hak Kyo Choi, Nag-Jin Cho, Seong-Keun Ryu, Joohyun Kang, Sunghyun Lee, Dong-Seok Chung, Hyung-Min Koo, Deog-Bon |
| description | Information obtained from peripheral blood could help us understand the underlying mechanisms in autoimmune diseases, cancer, pregnancy, and other conditions. In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression changes that occur during pregnancy using proteomic analysis. We detected 94 differentially expressed proteins in pregnant vs. non-pregnant (NP) pigs, and a representative set of the proteins was subjected to LC–MS/MS analysis. Furthermore, the identified proteins were categorized according to their biological process and molecular function. By classifying the proteins according to their functions, a large number of differentially regulated proteins involved in anti-oxidant, detoxification and stress response pathways were found, including peroxiredoxin (PRX) 1, 2, and 6, glutathione-S-transferase (GST), annexin A2, and A6, and heat shock protein 27 (HSP 27) during pregnancy (pregnancy d of E40, embryonic day 40; E70, embryonic day 70; and E93, embryonic day 93) compared with non-pregnancy. In this study, a proteomic approach utilizing 2-DE and LC–MS/MS was applied to evaluate specific molecular expression changes during pregnancy compared with non-pregnancy. Together, these data offer new information about the proteome map and factors that are differentially regulated during maintenance of normal pregnancy. |
| doi_str_mv | 10.1016/j.anireprosci.2012.07.008 |
| format | Article |
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In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression changes that occur during pregnancy using proteomic analysis. We detected 94 differentially expressed proteins in pregnant vs. non-pregnant (NP) pigs, and a representative set of the proteins was subjected to LC–MS/MS analysis. Furthermore, the identified proteins were categorized according to their biological process and molecular function. By classifying the proteins according to their functions, a large number of differentially regulated proteins involved in anti-oxidant, detoxification and stress response pathways were found, including peroxiredoxin (PRX) 1, 2, and 6, glutathione-S-transferase (GST), annexin A2, and A6, and heat shock protein 27 (HSP 27) during pregnancy (pregnancy d of E40, embryonic day 40; E70, embryonic day 70; and E93, embryonic day 93) compared with non-pregnancy. In this study, a proteomic approach utilizing 2-DE and LC–MS/MS was applied to evaluate specific molecular expression changes during pregnancy compared with non-pregnancy. Together, these data offer new information about the proteome map and factors that are differentially regulated during maintenance of normal pregnancy.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2012.07.008</identifier><identifier>PMID: 22917877</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; autoimmune diseases ; Blood Proteins - analysis ; Blood Proteins - classification ; Blood Proteins - metabolism ; Chromatography, Liquid ; Development ; Electrophoresis, Gel, Two-Dimensional ; Female ; gene expression regulation ; glutathione transferase ; heat shock proteins ; Leukocytes, Mononuclear - chemistry ; Leukocytes, Mononuclear - metabolism ; mononuclear leukocytes ; Peripheral blood mononuclear cells ; peroxiredoxin ; Pig ; Pregnancy ; Pregnancy Proteins - analysis ; Pregnancy Proteins - blood ; Pregnancy Proteins - classification ; Pregnancy Proteins - metabolism ; Pregnancy, Animal - blood ; Pregnancy, Animal - metabolism ; protein synthesis ; proteome ; Proteome - analysis ; Proteome - metabolism ; Proteomic ; proteomics ; Proteomics - methods ; stress response ; swine ; Swine - blood ; Swine - metabolism ; Swine - physiology ; Tandem Mass Spectrometry</subject><ispartof>Animal reproduction science, 2012-10, Vol.134 (3-4), p.164-176</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-a4e72e3f3ad089339637a6c2eb573fa9c327f89b54778f33a341df955dce0a083</citedby><cites>FETCH-LOGICAL-c425t-a4e72e3f3ad089339637a6c2eb573fa9c327f89b54778f33a341df955dce0a083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anireprosci.2012.07.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22917877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chae, Jung-Il</creatorcontrib><creatorcontrib>Kim, Jumi</creatorcontrib><creatorcontrib>Lee, Seong Goo</creatorcontrib><creatorcontrib>Koh, Min Whan</creatorcontrib><creatorcontrib>Jeon, Young-Joo</creatorcontrib><creatorcontrib>Kim, Dong-Wook</creatorcontrib><creatorcontrib>Ko, Seon mi</creatorcontrib><creatorcontrib>Seo, Kang Seok</creatorcontrib><creatorcontrib>Lee, Hak Kyo</creatorcontrib><creatorcontrib>Choi, Nag-Jin</creatorcontrib><creatorcontrib>Cho, Seong-Keun</creatorcontrib><creatorcontrib>Ryu, Joohyun</creatorcontrib><creatorcontrib>Kang, Sunghyun</creatorcontrib><creatorcontrib>Lee, Dong-Seok</creatorcontrib><creatorcontrib>Chung, Hyung-Min</creatorcontrib><creatorcontrib>Koo, Deog-Bon</creatorcontrib><title>Quantitative proteomic analysis of pregnancy-related proteins from peripheral blood mononuclear cells during pregnancy in pigs</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>Information obtained from peripheral blood could help us understand the underlying mechanisms in autoimmune diseases, cancer, pregnancy, and other conditions. In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression changes that occur during pregnancy using proteomic analysis. We detected 94 differentially expressed proteins in pregnant vs. non-pregnant (NP) pigs, and a representative set of the proteins was subjected to LC–MS/MS analysis. Furthermore, the identified proteins were categorized according to their biological process and molecular function. By classifying the proteins according to their functions, a large number of differentially regulated proteins involved in anti-oxidant, detoxification and stress response pathways were found, including peroxiredoxin (PRX) 1, 2, and 6, glutathione-S-transferase (GST), annexin A2, and A6, and heat shock protein 27 (HSP 27) during pregnancy (pregnancy d of E40, embryonic day 40; E70, embryonic day 70; and E93, embryonic day 93) compared with non-pregnancy. In this study, a proteomic approach utilizing 2-DE and LC–MS/MS was applied to evaluate specific molecular expression changes during pregnancy compared with non-pregnancy. Together, these data offer new information about the proteome map and factors that are differentially regulated during maintenance of normal pregnancy.</description><subject>Animals</subject><subject>autoimmune diseases</subject><subject>Blood Proteins - analysis</subject><subject>Blood Proteins - classification</subject><subject>Blood Proteins - metabolism</subject><subject>Chromatography, Liquid</subject><subject>Development</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Female</subject><subject>gene expression regulation</subject><subject>glutathione transferase</subject><subject>heat shock proteins</subject><subject>Leukocytes, Mononuclear - chemistry</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>mononuclear leukocytes</subject><subject>Peripheral blood mononuclear cells</subject><subject>peroxiredoxin</subject><subject>Pig</subject><subject>Pregnancy</subject><subject>Pregnancy Proteins - analysis</subject><subject>Pregnancy Proteins - blood</subject><subject>Pregnancy Proteins - classification</subject><subject>Pregnancy Proteins - metabolism</subject><subject>Pregnancy, Animal - blood</subject><subject>Pregnancy, Animal - metabolism</subject><subject>protein synthesis</subject><subject>proteome</subject><subject>Proteome - analysis</subject><subject>Proteome - metabolism</subject><subject>Proteomic</subject><subject>proteomics</subject><subject>Proteomics - methods</subject><subject>stress response</subject><subject>swine</subject><subject>Swine - blood</subject><subject>Swine - metabolism</subject><subject>Swine - physiology</subject><subject>Tandem Mass Spectrometry</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCXwBz45Lg2EnsHNGqUKRKCEHP1qwzXrxK7GAnlfbCb8ch5eOEOI1kPX7n1TOEvKpYWbGqfXMqwbuIUwzJuJKzipdMloypR2RXKSkKzgV_THZMSFXUgrMLcpnSiTEm27Z7Si447yqppNyR758W8LObYXb3SHPijGF0hoKH4ZxcosHmVzx68OZcRBxgxn7jnE_UxjDSCaObvmKEgR6GEHo6Bh_8YgaESA0OQ6L9Ep0__kmiztPJHdMz8sTCkPD5w7wid--uv-xvituP7z_s394WpubNXECNkqOwAnqmOiG6VkhoDcdDI4WFzggureoOTS2lskKAqKvedk3TG2TAlLgir7fc3PzbgmnWo0trNfAYlqQrprK4lncr2m2oyXpTRKun6EaI5wzpVb8-6b_061W_ZlKzn2tePKxZDiP2v3_-8p2BlxtgIWg4Rpf03eecUOfb1LVq-T8J3rZ8JfYbgVnYvcOocw30Bvtcysy6D-4_qv4ADfWybA</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Chae, Jung-Il</creator><creator>Kim, Jumi</creator><creator>Lee, Seong Goo</creator><creator>Koh, Min Whan</creator><creator>Jeon, Young-Joo</creator><creator>Kim, Dong-Wook</creator><creator>Ko, Seon mi</creator><creator>Seo, Kang Seok</creator><creator>Lee, Hak Kyo</creator><creator>Choi, Nag-Jin</creator><creator>Cho, Seong-Keun</creator><creator>Ryu, Joohyun</creator><creator>Kang, Sunghyun</creator><creator>Lee, Dong-Seok</creator><creator>Chung, Hyung-Min</creator><creator>Koo, Deog-Bon</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121001</creationdate><title>Quantitative proteomic analysis of pregnancy-related proteins from peripheral blood mononuclear cells during pregnancy in pigs</title><author>Chae, Jung-Il ; Kim, Jumi ; Lee, Seong Goo ; Koh, Min Whan ; Jeon, Young-Joo ; Kim, Dong-Wook ; Ko, Seon mi ; Seo, Kang Seok ; Lee, Hak Kyo ; Choi, Nag-Jin ; Cho, Seong-Keun ; Ryu, Joohyun ; Kang, Sunghyun ; Lee, Dong-Seok ; Chung, Hyung-Min ; Koo, Deog-Bon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-a4e72e3f3ad089339637a6c2eb573fa9c327f89b54778f33a341df955dce0a083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>autoimmune diseases</topic><topic>Blood Proteins - analysis</topic><topic>Blood Proteins - classification</topic><topic>Blood Proteins - metabolism</topic><topic>Chromatography, Liquid</topic><topic>Development</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Female</topic><topic>gene expression regulation</topic><topic>glutathione transferase</topic><topic>heat shock proteins</topic><topic>Leukocytes, Mononuclear - chemistry</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>mononuclear leukocytes</topic><topic>Peripheral blood mononuclear cells</topic><topic>peroxiredoxin</topic><topic>Pig</topic><topic>Pregnancy</topic><topic>Pregnancy Proteins - analysis</topic><topic>Pregnancy Proteins - blood</topic><topic>Pregnancy Proteins - classification</topic><topic>Pregnancy Proteins - metabolism</topic><topic>Pregnancy, Animal - blood</topic><topic>Pregnancy, Animal - metabolism</topic><topic>protein synthesis</topic><topic>proteome</topic><topic>Proteome - analysis</topic><topic>Proteome - metabolism</topic><topic>Proteomic</topic><topic>proteomics</topic><topic>Proteomics - methods</topic><topic>stress response</topic><topic>swine</topic><topic>Swine - blood</topic><topic>Swine - metabolism</topic><topic>Swine - physiology</topic><topic>Tandem Mass Spectrometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chae, Jung-Il</creatorcontrib><creatorcontrib>Kim, Jumi</creatorcontrib><creatorcontrib>Lee, Seong Goo</creatorcontrib><creatorcontrib>Koh, Min Whan</creatorcontrib><creatorcontrib>Jeon, Young-Joo</creatorcontrib><creatorcontrib>Kim, Dong-Wook</creatorcontrib><creatorcontrib>Ko, Seon mi</creatorcontrib><creatorcontrib>Seo, Kang Seok</creatorcontrib><creatorcontrib>Lee, Hak Kyo</creatorcontrib><creatorcontrib>Choi, Nag-Jin</creatorcontrib><creatorcontrib>Cho, Seong-Keun</creatorcontrib><creatorcontrib>Ryu, Joohyun</creatorcontrib><creatorcontrib>Kang, Sunghyun</creatorcontrib><creatorcontrib>Lee, Dong-Seok</creatorcontrib><creatorcontrib>Chung, Hyung-Min</creatorcontrib><creatorcontrib>Koo, Deog-Bon</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chae, Jung-Il</au><au>Kim, Jumi</au><au>Lee, Seong Goo</au><au>Koh, Min Whan</au><au>Jeon, Young-Joo</au><au>Kim, Dong-Wook</au><au>Ko, Seon mi</au><au>Seo, Kang Seok</au><au>Lee, Hak Kyo</au><au>Choi, Nag-Jin</au><au>Cho, Seong-Keun</au><au>Ryu, Joohyun</au><au>Kang, Sunghyun</au><au>Lee, Dong-Seok</au><au>Chung, Hyung-Min</au><au>Koo, Deog-Bon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative proteomic analysis of pregnancy-related proteins from peripheral blood mononuclear cells during pregnancy in pigs</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>134</volume><issue>3-4</issue><spage>164</spage><epage>176</epage><pages>164-176</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>Information obtained from peripheral blood could help us understand the underlying mechanisms in autoimmune diseases, cancer, pregnancy, and other conditions. In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression changes that occur during pregnancy using proteomic analysis. We detected 94 differentially expressed proteins in pregnant vs. non-pregnant (NP) pigs, and a representative set of the proteins was subjected to LC–MS/MS analysis. Furthermore, the identified proteins were categorized according to their biological process and molecular function. By classifying the proteins according to their functions, a large number of differentially regulated proteins involved in anti-oxidant, detoxification and stress response pathways were found, including peroxiredoxin (PRX) 1, 2, and 6, glutathione-S-transferase (GST), annexin A2, and A6, and heat shock protein 27 (HSP 27) during pregnancy (pregnancy d of E40, embryonic day 40; E70, embryonic day 70; and E93, embryonic day 93) compared with non-pregnancy. In this study, a proteomic approach utilizing 2-DE and LC–MS/MS was applied to evaluate specific molecular expression changes during pregnancy compared with non-pregnancy. Together, these data offer new information about the proteome map and factors that are differentially regulated during maintenance of normal pregnancy.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22917877</pmid><doi>10.1016/j.anireprosci.2012.07.008</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4320 |
| ispartof | Animal reproduction science, 2012-10, Vol.134 (3-4), p.164-176 |
| issn | 0378-4320 1873-2232 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals autoimmune diseases Blood Proteins - analysis Blood Proteins - classification Blood Proteins - metabolism Chromatography, Liquid Development Electrophoresis, Gel, Two-Dimensional Female gene expression regulation glutathione transferase heat shock proteins Leukocytes, Mononuclear - chemistry Leukocytes, Mononuclear - metabolism mononuclear leukocytes Peripheral blood mononuclear cells peroxiredoxin Pig Pregnancy Pregnancy Proteins - analysis Pregnancy Proteins - blood Pregnancy Proteins - classification Pregnancy Proteins - metabolism Pregnancy, Animal - blood Pregnancy, Animal - metabolism protein synthesis proteome Proteome - analysis Proteome - metabolism Proteomic proteomics Proteomics - methods stress response swine Swine - blood Swine - metabolism Swine - physiology Tandem Mass Spectrometry |
| title | Quantitative proteomic analysis of pregnancy-related proteins from peripheral blood mononuclear cells during pregnancy in pigs |
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