Comparative study of dye-loaded liposome accumulation in sensitive and resistant human breast cancer cells
The aim of this research is to study the dynamics and efficiency of liposome accumulation in sensitive and resistant human breast cancer cells. Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used. The liposome-to-cell interaction and dye cellular uptake in...
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| Veröffentlicht in: | Experimental oncology 2012-07, Vol.34 (2), p.101-106 |
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| container_title | Experimental oncology |
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| creator | Yefimova, S L Kurilchenko, I Yu Tkacheva, T N Rozhkov, V A Sorokin, A V Lukianova, N Yu Bezdenezhnykh, N A Malyukin, Yu V Chekhun, V F |
| description | The aim of this research is to study the dynamics and efficiency of liposome accumulation in sensitive and resistant human breast cancer cells.
Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used.
The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness.
Obtained results show that resistant cells accumulate dye-loaded liposomes more effectively and reveal more effective dye molecule cellular uptake. |
| format | Article |
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Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used.
The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness.
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Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used.
The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness.
Obtained results show that resistant cells accumulate dye-loaded liposomes more effectively and reveal more effective dye molecule cellular uptake.</description><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Breast Neoplasms - metabolism</subject><subject>Carbocyanines - chemistry</subject><subject>Carbocyanines - pharmacokinetics</subject><subject>Cisplatin - administration & dosage</subject><subject>Cisplatin - pharmacology</subject><subject>Doxorubicin - administration & dosage</subject><subject>Doxorubicin - pharmacology</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - pharmacokinetics</subject><subject>Drug Resistance, Neoplasm</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - pharmacokinetics</subject><subject>Humans</subject><subject>Lipid Bilayers - chemistry</subject><subject>Liposomes</subject><subject>MCF-7 Cells</subject><subject>Microscopy, Fluorescence</subject><subject>Molecular Structure</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Spectrometry, Fluorescence</subject><subject>Tissue Distribution</subject><issn>1812-9269</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kDtPwzAYRT2AaCn8BeSRJZJfseMRVbykSiwwR1_8EKliO9gOUv89BdrpLuceXd0LtKYdZY1mUq_QdSl7QmSrpbhCK8YJ5UrSNdpvU5ghQx2_HS51sQecPLYH10wJrLN4GudUUnAYjFnCMh3JFPEYcXGxjH81iBZnV8ZSIVb8uQSIeMgOSsUGonEZGzdN5QZdepiKuz3lBn08Pb5vX5rd2_Pr9mHXzJTJ2hiqrBVCt5wYSofWaOO58opR0F5RaoTsjJTCEt9xDawdZEdapYWnoLqB8A26__fOOX0trtQ-jOV3AUSXltJT0lHBmeLiiN6d0GUIzvZzHgPkQ3_-h_8AA5Jhkw</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Yefimova, S L</creator><creator>Kurilchenko, I Yu</creator><creator>Tkacheva, T N</creator><creator>Rozhkov, V A</creator><creator>Sorokin, A V</creator><creator>Lukianova, N Yu</creator><creator>Bezdenezhnykh, N A</creator><creator>Malyukin, Yu V</creator><creator>Chekhun, V F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201207</creationdate><title>Comparative study of dye-loaded liposome accumulation in sensitive and resistant human breast cancer cells</title><author>Yefimova, S L ; Kurilchenko, I Yu ; Tkacheva, T N ; Rozhkov, V A ; Sorokin, A V ; Lukianova, N Yu ; Bezdenezhnykh, N A ; Malyukin, Yu V ; Chekhun, V F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-c17dd449530c11b5c9cf37f721a9f711c468c664d0f839a25b6805794f1a78b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antineoplastic Agents - administration & dosage</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Breast Neoplasms - metabolism</topic><topic>Carbocyanines - chemistry</topic><topic>Carbocyanines - pharmacokinetics</topic><topic>Cisplatin - administration & dosage</topic><topic>Cisplatin - pharmacology</topic><topic>Doxorubicin - administration & dosage</topic><topic>Doxorubicin - pharmacology</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Carriers - pharmacokinetics</topic><topic>Drug Resistance, Neoplasm</topic><topic>Fluorescence Resonance Energy Transfer</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent Dyes - pharmacokinetics</topic><topic>Humans</topic><topic>Lipid Bilayers - chemistry</topic><topic>Liposomes</topic><topic>MCF-7 Cells</topic><topic>Microscopy, Fluorescence</topic><topic>Molecular Structure</topic><topic>Phosphatidylcholines - chemistry</topic><topic>Spectrometry, Fluorescence</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yefimova, S L</creatorcontrib><creatorcontrib>Kurilchenko, I Yu</creatorcontrib><creatorcontrib>Tkacheva, T N</creatorcontrib><creatorcontrib>Rozhkov, V A</creatorcontrib><creatorcontrib>Sorokin, A V</creatorcontrib><creatorcontrib>Lukianova, N Yu</creatorcontrib><creatorcontrib>Bezdenezhnykh, N A</creatorcontrib><creatorcontrib>Malyukin, Yu V</creatorcontrib><creatorcontrib>Chekhun, V F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yefimova, S L</au><au>Kurilchenko, I Yu</au><au>Tkacheva, T N</au><au>Rozhkov, V A</au><au>Sorokin, A V</au><au>Lukianova, N Yu</au><au>Bezdenezhnykh, N A</au><au>Malyukin, Yu V</au><au>Chekhun, V F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative study of dye-loaded liposome accumulation in sensitive and resistant human breast cancer cells</atitle><jtitle>Experimental oncology</jtitle><addtitle>Exp Oncol</addtitle><date>2012-07</date><risdate>2012</risdate><volume>34</volume><issue>2</issue><spage>101</spage><epage>106</epage><pages>101-106</pages><issn>1812-9269</issn><abstract>The aim of this research is to study the dynamics and efficiency of liposome accumulation in sensitive and resistant human breast cancer cells.
Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used.
The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness.
Obtained results show that resistant cells accumulate dye-loaded liposomes more effectively and reveal more effective dye molecule cellular uptake.</abstract><cop>Ukraine</cop><pmid>23013761</pmid><tpages>6</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antineoplastic Agents - administration & dosage Antineoplastic Agents - pharmacology Breast Neoplasms - metabolism Carbocyanines - chemistry Carbocyanines - pharmacokinetics Cisplatin - administration & dosage Cisplatin - pharmacology Doxorubicin - administration & dosage Doxorubicin - pharmacology Drug Carriers - chemistry Drug Carriers - pharmacokinetics Drug Resistance, Neoplasm Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemistry Fluorescent Dyes - pharmacokinetics Humans Lipid Bilayers - chemistry Liposomes MCF-7 Cells Microscopy, Fluorescence Molecular Structure Phosphatidylcholines - chemistry Spectrometry, Fluorescence Tissue Distribution |
| title | Comparative study of dye-loaded liposome accumulation in sensitive and resistant human breast cancer cells |
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