Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo
Summary Background The barrier perturbation pattern and molecular markers of inflammation upon tandem repeated irritation in chronologically aged skin have not been previously studied. Objectives We aimed to investigate the barrier impairment kinetic and in vivo cytokine profile following sequenti...
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| Veröffentlicht in: | British journal of dermatology (1951) 2012-10, Vol.167 (4), p.787-793 |
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| container_title | British journal of dermatology (1951) |
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| creator | Angelova-Fischer, I. Becker, V. Fischer, T.W. Zillikens, D. Wigger-Alberti, W. Kezic, S. |
| description | Summary
Background The barrier perturbation pattern and molecular markers of inflammation upon tandem repeated irritation in chronologically aged skin have not been previously studied.
Objectives We aimed to investigate the barrier impairment kinetic and in vivo cytokine profile following sequential irritation with sodium lauryl sulfate (SLS) and undiluted toluene (Tol) in aged compared with young skin.
Methods Four fields on the volar forearm of healthy aged and young volunteers (median age, respectively, 63·9 and 32·6 years) were sequentially exposed to 0·5% SLS and undiluted toluene in a controlled tandem repeated irritation test; an adjacent nontreated field served as control. The permeability barrier function was monitored by repeated measurements of transepidermal water loss (TEWL), capacitance and erythema every 24 h up to 96 h. The stratum corneum cytokines were harvested by sequential tape stripping and quantified by multiplex bead array and enzyme‐linked immunosorbent assay.
Results Compared with young skin, aged skin was characterized by delayed and/or less pronounced alterations in the visual irritation score, TEWL, chromametry a*‐value and capacitance, assessed by the respective Δ‐values for each parameter and monitoring time point. In both groups, exposure to SLS/SLS, SLS/Tol and Tol/SLS resulted in decreased interleukin (IL)‐1α levels, whereas the application of Tol/Tol induced an increase in IL‐1α. Furthermore, decreased IL‐1 receptor antagonist (IL‐1RA) levels and a lower IL‐1RA/IL‐1α ratio were found following repeated exposure to the irritants.
Conclusions Our results provide evidence for selective alterations in the cytokine profile and distinct barrier impairment kinetic following tandem repeated irritation with SLS and Tol in aged compared with young skin in vivo. |
| doi_str_mv | 10.1111/j.1365-2133.2012.11049.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1081432305</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1081432305</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3899-684a1467dc17a00dc55722da1c2f9ce551257e592d7404195115705b64d86e9b3</originalsourceid><addsrcrecordid>eNpFkVFv0zAQxy3ExMrGV0B-QeIl5WzHcfyCxDYoTAOkaYhHy7GvyF2adHYy2m-Ps3bFL_bd_e5v-_6EUAZzlteH1ZyJShacCTHnwHjOQqnn2xdkdiy8JDMAUAXoSpyS1ymtAJgACa_IKedS1ZyzGVnd2c7jmkbcoB3Q0xBjGOwQ-o6Gjto_OZXuwxT40WGiPqQhdG6gjc0kRrrBOIyx2bdkMep2Q587kG5ivwwtTjqP4bE_JydL2yZ8c9jPyK8vn-8uvxY3PxffLj_dFE7UWhdVXVpWVso7piyAd1Iqzr1lji-1QylZfjxKzb0qoWRaMiYVyKYqfV2hbsQZeb_Xzfc_jJgGsw7JYdvaDvsxGQY1KwXPo8jo2wM6Nmv0ZhPD2sadeZ5PBt4dAJucbZfRdi6k_1wloRa6ztzHPfc3f3h3rDMwk19mZSZbzGSLmfwyT36Zrbm4vno6ZoFiL5DHi9ujgI33plJCSfP7x8Jcywu-uJLfza34BxDJlzU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1081432305</pqid></control><display><type>article</type><title>Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Angelova-Fischer, I. ; Becker, V. ; Fischer, T.W. ; Zillikens, D. ; Wigger-Alberti, W. ; Kezic, S.</creator><creatorcontrib>Angelova-Fischer, I. ; Becker, V. ; Fischer, T.W. ; Zillikens, D. ; Wigger-Alberti, W. ; Kezic, S.</creatorcontrib><description>Summary
Background The barrier perturbation pattern and molecular markers of inflammation upon tandem repeated irritation in chronologically aged skin have not been previously studied.
Objectives We aimed to investigate the barrier impairment kinetic and in vivo cytokine profile following sequential irritation with sodium lauryl sulfate (SLS) and undiluted toluene (Tol) in aged compared with young skin.
Methods Four fields on the volar forearm of healthy aged and young volunteers (median age, respectively, 63·9 and 32·6 years) were sequentially exposed to 0·5% SLS and undiluted toluene in a controlled tandem repeated irritation test; an adjacent nontreated field served as control. The permeability barrier function was monitored by repeated measurements of transepidermal water loss (TEWL), capacitance and erythema every 24 h up to 96 h. The stratum corneum cytokines were harvested by sequential tape stripping and quantified by multiplex bead array and enzyme‐linked immunosorbent assay.
Results Compared with young skin, aged skin was characterized by delayed and/or less pronounced alterations in the visual irritation score, TEWL, chromametry a*‐value and capacitance, assessed by the respective Δ‐values for each parameter and monitoring time point. In both groups, exposure to SLS/SLS, SLS/Tol and Tol/SLS resulted in decreased interleukin (IL)‐1α levels, whereas the application of Tol/Tol induced an increase in IL‐1α. Furthermore, decreased IL‐1 receptor antagonist (IL‐1RA) levels and a lower IL‐1RA/IL‐1α ratio were found following repeated exposure to the irritants.
Conclusions Our results provide evidence for selective alterations in the cytokine profile and distinct barrier impairment kinetic following tandem repeated irritation with SLS and Tol in aged compared with young skin in vivo.</description><identifier>ISSN: 0007-0963</identifier><identifier>EISSN: 1365-2133</identifier><identifier>DOI: 10.1111/j.1365-2133.2012.11049.x</identifier><identifier>PMID: 22578221</identifier><identifier>CODEN: BJDEAZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Body Water - metabolism ; Cytokines - metabolism ; Dermatitis, Irritant - etiology ; Dermatitis, Irritant - metabolism ; Dermatology ; Enzyme-Linked Immunosorbent Assay ; Epidermis - drug effects ; Epidermis - metabolism ; Erythema - chemically induced ; Female ; Humans ; Interleukin-1alpha - metabolism ; Male ; Medical sciences ; Middle Aged ; Receptors, Interleukin-1 Type I - antagonists & inhibitors ; Receptors, Interleukin-1 Type I - metabolism ; Skin - drug effects ; Skin Aging - drug effects ; Sodium Dodecyl Sulfate - toxicity ; Solvents - toxicity ; Surface-Active Agents - toxicity ; Toluene - toxicity ; Young Adult</subject><ispartof>British journal of dermatology (1951), 2012-10, Vol.167 (4), p.787-793</ispartof><rights>2012 The Authors. BJD © 2012 British Association of Dermatologists</rights><rights>2015 INIST-CNRS</rights><rights>2012 The Authors. BJD © 2012 British Association of Dermatologists.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3899-684a1467dc17a00dc55722da1c2f9ce551257e592d7404195115705b64d86e9b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2133.2012.11049.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2133.2012.11049.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26508398$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22578221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angelova-Fischer, I.</creatorcontrib><creatorcontrib>Becker, V.</creatorcontrib><creatorcontrib>Fischer, T.W.</creatorcontrib><creatorcontrib>Zillikens, D.</creatorcontrib><creatorcontrib>Wigger-Alberti, W.</creatorcontrib><creatorcontrib>Kezic, S.</creatorcontrib><title>Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo</title><title>British journal of dermatology (1951)</title><addtitle>Br J Dermatol</addtitle><description>Summary
Background The barrier perturbation pattern and molecular markers of inflammation upon tandem repeated irritation in chronologically aged skin have not been previously studied.
Objectives We aimed to investigate the barrier impairment kinetic and in vivo cytokine profile following sequential irritation with sodium lauryl sulfate (SLS) and undiluted toluene (Tol) in aged compared with young skin.
Methods Four fields on the volar forearm of healthy aged and young volunteers (median age, respectively, 63·9 and 32·6 years) were sequentially exposed to 0·5% SLS and undiluted toluene in a controlled tandem repeated irritation test; an adjacent nontreated field served as control. The permeability barrier function was monitored by repeated measurements of transepidermal water loss (TEWL), capacitance and erythema every 24 h up to 96 h. The stratum corneum cytokines were harvested by sequential tape stripping and quantified by multiplex bead array and enzyme‐linked immunosorbent assay.
Results Compared with young skin, aged skin was characterized by delayed and/or less pronounced alterations in the visual irritation score, TEWL, chromametry a*‐value and capacitance, assessed by the respective Δ‐values for each parameter and monitoring time point. In both groups, exposure to SLS/SLS, SLS/Tol and Tol/SLS resulted in decreased interleukin (IL)‐1α levels, whereas the application of Tol/Tol induced an increase in IL‐1α. Furthermore, decreased IL‐1 receptor antagonist (IL‐1RA) levels and a lower IL‐1RA/IL‐1α ratio were found following repeated exposure to the irritants.
Conclusions Our results provide evidence for selective alterations in the cytokine profile and distinct barrier impairment kinetic following tandem repeated irritation with SLS and Tol in aged compared with young skin in vivo.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Body Water - metabolism</subject><subject>Cytokines - metabolism</subject><subject>Dermatitis, Irritant - etiology</subject><subject>Dermatitis, Irritant - metabolism</subject><subject>Dermatology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epidermis - drug effects</subject><subject>Epidermis - metabolism</subject><subject>Erythema - chemically induced</subject><subject>Female</subject><subject>Humans</subject><subject>Interleukin-1alpha - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Receptors, Interleukin-1 Type I - antagonists & inhibitors</subject><subject>Receptors, Interleukin-1 Type I - metabolism</subject><subject>Skin - drug effects</subject><subject>Skin Aging - drug effects</subject><subject>Sodium Dodecyl Sulfate - toxicity</subject><subject>Solvents - toxicity</subject><subject>Surface-Active Agents - toxicity</subject><subject>Toluene - toxicity</subject><subject>Young Adult</subject><issn>0007-0963</issn><issn>1365-2133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkVFv0zAQxy3ExMrGV0B-QeIl5WzHcfyCxDYoTAOkaYhHy7GvyF2adHYy2m-Ps3bFL_bd_e5v-_6EUAZzlteH1ZyJShacCTHnwHjOQqnn2xdkdiy8JDMAUAXoSpyS1ymtAJgACa_IKedS1ZyzGVnd2c7jmkbcoB3Q0xBjGOwQ-o6Gjto_OZXuwxT40WGiPqQhdG6gjc0kRrrBOIyx2bdkMep2Q587kG5ivwwtTjqP4bE_JydL2yZ8c9jPyK8vn-8uvxY3PxffLj_dFE7UWhdVXVpWVso7piyAd1Iqzr1lji-1QylZfjxKzb0qoWRaMiYVyKYqfV2hbsQZeb_Xzfc_jJgGsw7JYdvaDvsxGQY1KwXPo8jo2wM6Nmv0ZhPD2sadeZ5PBt4dAJucbZfRdi6k_1wloRa6ztzHPfc3f3h3rDMwk19mZSZbzGSLmfwyT36Zrbm4vno6ZoFiL5DHi9ujgI33plJCSfP7x8Jcywu-uJLfza34BxDJlzU</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>Angelova-Fischer, I.</creator><creator>Becker, V.</creator><creator>Fischer, T.W.</creator><creator>Zillikens, D.</creator><creator>Wigger-Alberti, W.</creator><creator>Kezic, S.</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201210</creationdate><title>Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo</title><author>Angelova-Fischer, I. ; Becker, V. ; Fischer, T.W. ; Zillikens, D. ; Wigger-Alberti, W. ; Kezic, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3899-684a1467dc17a00dc55722da1c2f9ce551257e592d7404195115705b64d86e9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Body Water - metabolism</topic><topic>Cytokines - metabolism</topic><topic>Dermatitis, Irritant - etiology</topic><topic>Dermatitis, Irritant - metabolism</topic><topic>Dermatology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epidermis - drug effects</topic><topic>Epidermis - metabolism</topic><topic>Erythema - chemically induced</topic><topic>Female</topic><topic>Humans</topic><topic>Interleukin-1alpha - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Receptors, Interleukin-1 Type I - antagonists & inhibitors</topic><topic>Receptors, Interleukin-1 Type I - metabolism</topic><topic>Skin - drug effects</topic><topic>Skin Aging - drug effects</topic><topic>Sodium Dodecyl Sulfate - toxicity</topic><topic>Solvents - toxicity</topic><topic>Surface-Active Agents - toxicity</topic><topic>Toluene - toxicity</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angelova-Fischer, I.</creatorcontrib><creatorcontrib>Becker, V.</creatorcontrib><creatorcontrib>Fischer, T.W.</creatorcontrib><creatorcontrib>Zillikens, D.</creatorcontrib><creatorcontrib>Wigger-Alberti, W.</creatorcontrib><creatorcontrib>Kezic, S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of dermatology (1951)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angelova-Fischer, I.</au><au>Becker, V.</au><au>Fischer, T.W.</au><au>Zillikens, D.</au><au>Wigger-Alberti, W.</au><au>Kezic, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo</atitle><jtitle>British journal of dermatology (1951)</jtitle><addtitle>Br J Dermatol</addtitle><date>2012-10</date><risdate>2012</risdate><volume>167</volume><issue>4</issue><spage>787</spage><epage>793</epage><pages>787-793</pages><issn>0007-0963</issn><eissn>1365-2133</eissn><coden>BJDEAZ</coden><abstract>Summary
Background The barrier perturbation pattern and molecular markers of inflammation upon tandem repeated irritation in chronologically aged skin have not been previously studied.
Objectives We aimed to investigate the barrier impairment kinetic and in vivo cytokine profile following sequential irritation with sodium lauryl sulfate (SLS) and undiluted toluene (Tol) in aged compared with young skin.
Methods Four fields on the volar forearm of healthy aged and young volunteers (median age, respectively, 63·9 and 32·6 years) were sequentially exposed to 0·5% SLS and undiluted toluene in a controlled tandem repeated irritation test; an adjacent nontreated field served as control. The permeability barrier function was monitored by repeated measurements of transepidermal water loss (TEWL), capacitance and erythema every 24 h up to 96 h. The stratum corneum cytokines were harvested by sequential tape stripping and quantified by multiplex bead array and enzyme‐linked immunosorbent assay.
Results Compared with young skin, aged skin was characterized by delayed and/or less pronounced alterations in the visual irritation score, TEWL, chromametry a*‐value and capacitance, assessed by the respective Δ‐values for each parameter and monitoring time point. In both groups, exposure to SLS/SLS, SLS/Tol and Tol/SLS resulted in decreased interleukin (IL)‐1α levels, whereas the application of Tol/Tol induced an increase in IL‐1α. Furthermore, decreased IL‐1 receptor antagonist (IL‐1RA) levels and a lower IL‐1RA/IL‐1α ratio were found following repeated exposure to the irritants.
Conclusions Our results provide evidence for selective alterations in the cytokine profile and distinct barrier impairment kinetic following tandem repeated irritation with SLS and Tol in aged compared with young skin in vivo.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22578221</pmid><doi>10.1111/j.1365-2133.2012.11049.x</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adult Aged Biological and medical sciences Body Water - metabolism Cytokines - metabolism Dermatitis, Irritant - etiology Dermatitis, Irritant - metabolism Dermatology Enzyme-Linked Immunosorbent Assay Epidermis - drug effects Epidermis - metabolism Erythema - chemically induced Female Humans Interleukin-1alpha - metabolism Male Medical sciences Middle Aged Receptors, Interleukin-1 Type I - antagonists & inhibitors Receptors, Interleukin-1 Type I - metabolism Skin - drug effects Skin Aging - drug effects Sodium Dodecyl Sulfate - toxicity Solvents - toxicity Surface-Active Agents - toxicity Toluene - toxicity Young Adult |
| title | Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo |
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