A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation
Carbon monoxide is a member of the gasotransmitter family, which also includes NO and H2S, and has been implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well-studied, the real-time spatial and t...
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| Veröffentlicht in: | Journal of the American Chemical Society 2012-09, Vol.134 (38), p.15668-15671 |
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| container_end_page | 15671 |
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| container_issue | 38 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 134 |
| creator | Michel, Brian W Lippert, Alexander R Chang, Christopher J |
| description | Carbon monoxide is a member of the gasotransmitter family, which also includes NO and H2S, and has been implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well-studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. Here we report a new type of turn-on fluorescent probe for selective CO detection based on palladium-mediated carbonylation reactivity. CO Probe 1 (COP-1) is capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems. |
| doi_str_mv | 10.1021/ja307017b |
| format | Article |
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Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well-studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. Here we report a new type of turn-on fluorescent probe for selective CO detection based on palladium-mediated carbonylation reactivity. CO Probe 1 (COP-1) is capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja307017b</identifier><identifier>PMID: 22970765</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Boron Compounds - chemistry ; Carbon Monoxide - analysis ; Coordination Complexes - chemistry ; Fluorescent Dyes - chemistry ; Microscopy, Confocal ; Palladium - chemistry</subject><ispartof>Journal of the American Chemical Society, 2012-09, Vol.134 (38), p.15668-15671</ispartof><rights>Copyright © 2012 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a381t-b7151970433980027b8250d647661800024b408ccafad340a86c4212057d2bcf3</citedby><cites>FETCH-LOGICAL-a381t-b7151970433980027b8250d647661800024b408ccafad340a86c4212057d2bcf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja307017b$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja307017b$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22970765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Michel, Brian W</creatorcontrib><creatorcontrib>Lippert, Alexander R</creatorcontrib><creatorcontrib>Chang, Christopher J</creatorcontrib><title>A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation</title><title>Journal of the American Chemical Society</title><addtitle>J. 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CO Probe 1 (COP-1) is capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.</description><subject>Animals</subject><subject>Boron Compounds - chemistry</subject><subject>Carbon Monoxide - analysis</subject><subject>Coordination Complexes - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Microscopy, Confocal</subject><subject>Palladium - chemistry</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkD9PwzAQxS0EoqUw8AWQFyQYArbzx-5YIgqVWlEBnaNL4lSukrjYSUV3PjiOUjox-az73bt7D6FrSh4oYfRxAz7hhPL0BA1pyIgXUhadoiEhhHlcRP4AXVi7cd-ACXqOBoyNOeFROEQ_E_wuIWuUrr0nsDLH07LVRtpM1g1eGp1KXGiDP2QpHbWTeFbBWtVrrAscg0l1jRe61t8ql1jVeK52XTOWZWnxynY14CWUJeSqrbyFzBU0bks_ui-h23yJzgoorbw6vCO0mj5_xq_e_O1lFk_mHviCNl7KaUjd4YHvj4WzxlPBQpJHAY8iKjqzQRoQkWVQQO4HBESUBYwyEvKcpVnhj9Bdr7s1-quVtkkq5Yy642qpW5tQIogQPqHUofc9mhltrZFFsjWqArN3UNKFnhxDd-zNQbZNK5kfyb-UHXDbA5DZZKNbUzuX_wj9AhCjhx8</recordid><startdate>20120926</startdate><enddate>20120926</enddate><creator>Michel, Brian W</creator><creator>Lippert, Alexander R</creator><creator>Chang, Christopher J</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120926</creationdate><title>A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation</title><author>Michel, Brian W ; Lippert, Alexander R ; Chang, Christopher J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a381t-b7151970433980027b8250d647661800024b408ccafad340a86c4212057d2bcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Boron Compounds - chemistry</topic><topic>Carbon Monoxide - analysis</topic><topic>Coordination Complexes - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Microscopy, Confocal</topic><topic>Palladium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michel, Brian W</creatorcontrib><creatorcontrib>Lippert, Alexander R</creatorcontrib><creatorcontrib>Chang, Christopher J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michel, Brian W</au><au>Lippert, Alexander R</au><au>Chang, Christopher J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. 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| issn | 0002-7863 1520-5126 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Boron Compounds - chemistry Carbon Monoxide - analysis Coordination Complexes - chemistry Fluorescent Dyes - chemistry Microscopy, Confocal Palladium - chemistry |
| title | A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation |
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