Computer-assisted anatomic dissection (CAAD): evolution, methodology and application in intra-pelvic innervation study
Objective Classic anatomical methods have limitations in micro determination of nerve fibre location. Furthermore, the precise detection of the nerve fibres nature is not possible by means of dissection. The combination of immunohistochemistry and three-dimensional reconstruction could be used to re...
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| Veröffentlicht in: | Surgical and radiologic anatomy (English ed.) 2012-10, Vol.34 (8), p.721-729 |
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| creator | Alsaid, Bayan Bessede, Thomas Diallo, Djibril Karam, Ibrahim Uhl, Jean François Delmas, Vincent Droupy, Stéphane Benoît, Gérard |
| description | Objective
Classic anatomical methods have limitations in micro determination of nerve fibre location. Furthermore, the precise detection of the nerve fibres nature is not possible by means of dissection. The combination of immunohistochemistry and three-dimensional reconstruction could be used to resolve these limitations of morphological sciences. Our aim is to describe the evolution of computer-assisted anatomic dissection (CAAD), which is an original method applied to study the distribution of intra-pelvic nerves in anatomic research.
Materials and methods
Serial transverse sectioning of the pelvic region in rabbit, human fetus, infant and adult cadaver was performed. Sections were immuno-histochemically stained and digitized with a high optical resolution scanner. Photoshop 7 software was used in regrouping of the adult cadaver sections then a tri-dimensional reconstruction was achieved using WinSurf software.
Results
The 3D reconstruction of the immuno-histochemically stained histologic sections of the pelvis allowed for precise structural identification of the prostate and its innervations (in fetus, infant and adult). In addition, we reconstructed the entire intra-pelvic organs with accurate demonstration of the location of both adrenergic and cholinergic pathways. Moreover, we performed a virtual dissection of each of the pelvic structures with description of the exact location of the inferior hypogastric plexus, as well as the nature and the distribution of its fibres.
Conclusion
The CAAD is an original method in anatomic research, which illustrates the fact that descriptive anatomy is still a dynamic science. This method allows for a 3D presentation of the intra-organic innervation, the nature of the nerve fibres, and the distribution of receptors and their neurotransmitters. This technique improves the understanding of the complex anatomic regions such as the pelvis from both surgical and educational point of view. |
| doi_str_mv | 10.1007/s00276-011-0855-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1074783474</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1074783474</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-b88d046810f682905eea333ab19845bbf9a6ab3271e20f16e781e5a2e78ee1113</originalsourceid><addsrcrecordid>eNp1kV-L1TAQxYMo7nX1A_giBV9WMOtM0jTpvl2uf1ZY2Bd9Lmk7Xbu0TU3SC_fbm9pVRFgYGJj5nTMDh7HXCJcIoD8EAKELDogcjFIcn7AdCl1yo1A_ZTsoJXAEac7YixDuAUAhmufsTKDWZSnMjh0PbpyXSJ7bEPoQqc3sZKMb-yZr-xCoib2bsovDfv_x3VVGRzcs6-R9NlL84Vo3uLtTkiTZPA99Y3_j_VrRWz7TcExO_TSRP267EJf29JI96-wQ6NVDP2ffP3_6drjmN7dfvh72N7yRWkReG9NCXhiErjCiBEVkpZS2xtLkqq670ha2lkIjCeiwIG2QlBWpEyGiPGcXm-_s3c-FQqzGPjQ0DHYit4QKQefayFznCX37H3rvFj-l7xKVl0oJlUOicKMa70Lw1FWz70frTwmq1lCqLZQqhVKtoVTrE28enJd6pPav4k8KCRAbENJquiP_7-nHXH8BGbCXsA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1049552540</pqid></control><display><type>article</type><title>Computer-assisted anatomic dissection (CAAD): evolution, methodology and application in intra-pelvic innervation study</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Alsaid, Bayan ; Bessede, Thomas ; Diallo, Djibril ; Karam, Ibrahim ; Uhl, Jean François ; Delmas, Vincent ; Droupy, Stéphane ; Benoît, Gérard</creator><creatorcontrib>Alsaid, Bayan ; Bessede, Thomas ; Diallo, Djibril ; Karam, Ibrahim ; Uhl, Jean François ; Delmas, Vincent ; Droupy, Stéphane ; Benoît, Gérard</creatorcontrib><description>Objective
Classic anatomical methods have limitations in micro determination of nerve fibre location. Furthermore, the precise detection of the nerve fibres nature is not possible by means of dissection. The combination of immunohistochemistry and three-dimensional reconstruction could be used to resolve these limitations of morphological sciences. Our aim is to describe the evolution of computer-assisted anatomic dissection (CAAD), which is an original method applied to study the distribution of intra-pelvic nerves in anatomic research.
Materials and methods
Serial transverse sectioning of the pelvic region in rabbit, human fetus, infant and adult cadaver was performed. Sections were immuno-histochemically stained and digitized with a high optical resolution scanner. Photoshop 7 software was used in regrouping of the adult cadaver sections then a tri-dimensional reconstruction was achieved using WinSurf software.
Results
The 3D reconstruction of the immuno-histochemically stained histologic sections of the pelvis allowed for precise structural identification of the prostate and its innervations (in fetus, infant and adult). In addition, we reconstructed the entire intra-pelvic organs with accurate demonstration of the location of both adrenergic and cholinergic pathways. Moreover, we performed a virtual dissection of each of the pelvic structures with description of the exact location of the inferior hypogastric plexus, as well as the nature and the distribution of its fibres.
Conclusion
The CAAD is an original method in anatomic research, which illustrates the fact that descriptive anatomy is still a dynamic science. This method allows for a 3D presentation of the intra-organic innervation, the nature of the nerve fibres, and the distribution of receptors and their neurotransmitters. This technique improves the understanding of the complex anatomic regions such as the pelvis from both surgical and educational point of view.</description><identifier>ISSN: 0930-1038</identifier><identifier>EISSN: 1279-8517</identifier><identifier>DOI: 10.1007/s00276-011-0855-1</identifier><identifier>PMID: 21779928</identifier><language>eng</language><publisher>Paris: Springer-Verlag</publisher><subject>3-D technology ; Adult ; Anatomy ; Anatomy & physiology ; Animals ; Cadaver ; Dissection ; Dissection - methods ; Fetus ; Humans ; Image Processing, Computer-Assisted - methods ; Imaging ; Imaging, Three-Dimensional - methods ; Infant ; Medicine ; Medicine & Public Health ; Nervous system ; Original Article ; Orthopedics ; Pelvis - innervation ; Rabbits ; Radiology ; Surgery</subject><ispartof>Surgical and radiologic anatomy (English ed.), 2012-10, Vol.34 (8), p.721-729</ispartof><rights>Springer-Verlag 2011</rights><rights>Springer-Verlag France 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-b88d046810f682905eea333ab19845bbf9a6ab3271e20f16e781e5a2e78ee1113</citedby><cites>FETCH-LOGICAL-c372t-b88d046810f682905eea333ab19845bbf9a6ab3271e20f16e781e5a2e78ee1113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00276-011-0855-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00276-011-0855-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21779928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alsaid, Bayan</creatorcontrib><creatorcontrib>Bessede, Thomas</creatorcontrib><creatorcontrib>Diallo, Djibril</creatorcontrib><creatorcontrib>Karam, Ibrahim</creatorcontrib><creatorcontrib>Uhl, Jean François</creatorcontrib><creatorcontrib>Delmas, Vincent</creatorcontrib><creatorcontrib>Droupy, Stéphane</creatorcontrib><creatorcontrib>Benoît, Gérard</creatorcontrib><title>Computer-assisted anatomic dissection (CAAD): evolution, methodology and application in intra-pelvic innervation study</title><title>Surgical and radiologic anatomy (English ed.)</title><addtitle>Surg Radiol Anat</addtitle><addtitle>Surg Radiol Anat</addtitle><description>Objective
Classic anatomical methods have limitations in micro determination of nerve fibre location. Furthermore, the precise detection of the nerve fibres nature is not possible by means of dissection. The combination of immunohistochemistry and three-dimensional reconstruction could be used to resolve these limitations of morphological sciences. Our aim is to describe the evolution of computer-assisted anatomic dissection (CAAD), which is an original method applied to study the distribution of intra-pelvic nerves in anatomic research.
Materials and methods
Serial transverse sectioning of the pelvic region in rabbit, human fetus, infant and adult cadaver was performed. Sections were immuno-histochemically stained and digitized with a high optical resolution scanner. Photoshop 7 software was used in regrouping of the adult cadaver sections then a tri-dimensional reconstruction was achieved using WinSurf software.
Results
The 3D reconstruction of the immuno-histochemically stained histologic sections of the pelvis allowed for precise structural identification of the prostate and its innervations (in fetus, infant and adult). In addition, we reconstructed the entire intra-pelvic organs with accurate demonstration of the location of both adrenergic and cholinergic pathways. Moreover, we performed a virtual dissection of each of the pelvic structures with description of the exact location of the inferior hypogastric plexus, as well as the nature and the distribution of its fibres.
Conclusion
The CAAD is an original method in anatomic research, which illustrates the fact that descriptive anatomy is still a dynamic science. This method allows for a 3D presentation of the intra-organic innervation, the nature of the nerve fibres, and the distribution of receptors and their neurotransmitters. This technique improves the understanding of the complex anatomic regions such as the pelvis from both surgical and educational point of view.</description><subject>3-D technology</subject><subject>Adult</subject><subject>Anatomy</subject><subject>Anatomy & physiology</subject><subject>Animals</subject><subject>Cadaver</subject><subject>Dissection</subject><subject>Dissection - methods</subject><subject>Fetus</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Imaging</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Infant</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nervous system</subject><subject>Original Article</subject><subject>Orthopedics</subject><subject>Pelvis - innervation</subject><subject>Rabbits</subject><subject>Radiology</subject><subject>Surgery</subject><issn>0930-1038</issn><issn>1279-8517</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kV-L1TAQxYMo7nX1A_giBV9WMOtM0jTpvl2uf1ZY2Bd9Lmk7Xbu0TU3SC_fbm9pVRFgYGJj5nTMDh7HXCJcIoD8EAKELDogcjFIcn7AdCl1yo1A_ZTsoJXAEac7YixDuAUAhmufsTKDWZSnMjh0PbpyXSJ7bEPoQqc3sZKMb-yZr-xCoib2bsovDfv_x3VVGRzcs6-R9NlL84Vo3uLtTkiTZPA99Y3_j_VrRWz7TcExO_TSRP267EJf29JI96-wQ6NVDP2ffP3_6drjmN7dfvh72N7yRWkReG9NCXhiErjCiBEVkpZS2xtLkqq670ha2lkIjCeiwIG2QlBWpEyGiPGcXm-_s3c-FQqzGPjQ0DHYit4QKQefayFznCX37H3rvFj-l7xKVl0oJlUOicKMa70Lw1FWz70frTwmq1lCqLZQqhVKtoVTrE28enJd6pPav4k8KCRAbENJquiP_7-nHXH8BGbCXsA</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Alsaid, Bayan</creator><creator>Bessede, Thomas</creator><creator>Diallo, Djibril</creator><creator>Karam, Ibrahim</creator><creator>Uhl, Jean François</creator><creator>Delmas, Vincent</creator><creator>Droupy, Stéphane</creator><creator>Benoît, Gérard</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20121001</creationdate><title>Computer-assisted anatomic dissection (CAAD): evolution, methodology and application in intra-pelvic innervation study</title><author>Alsaid, Bayan ; Bessede, Thomas ; Diallo, Djibril ; Karam, Ibrahim ; Uhl, Jean François ; Delmas, Vincent ; Droupy, Stéphane ; Benoît, Gérard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-b88d046810f682905eea333ab19845bbf9a6ab3271e20f16e781e5a2e78ee1113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>3-D technology</topic><topic>Adult</topic><topic>Anatomy</topic><topic>Anatomy & physiology</topic><topic>Animals</topic><topic>Cadaver</topic><topic>Dissection</topic><topic>Dissection - methods</topic><topic>Fetus</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>Imaging</topic><topic>Imaging, Three-Dimensional - methods</topic><topic>Infant</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nervous system</topic><topic>Original Article</topic><topic>Orthopedics</topic><topic>Pelvis - innervation</topic><topic>Rabbits</topic><topic>Radiology</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alsaid, Bayan</creatorcontrib><creatorcontrib>Bessede, Thomas</creatorcontrib><creatorcontrib>Diallo, Djibril</creatorcontrib><creatorcontrib>Karam, Ibrahim</creatorcontrib><creatorcontrib>Uhl, Jean François</creatorcontrib><creatorcontrib>Delmas, Vincent</creatorcontrib><creatorcontrib>Droupy, Stéphane</creatorcontrib><creatorcontrib>Benoît, Gérard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Surgical and radiologic anatomy (English ed.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alsaid, Bayan</au><au>Bessede, Thomas</au><au>Diallo, Djibril</au><au>Karam, Ibrahim</au><au>Uhl, Jean François</au><au>Delmas, Vincent</au><au>Droupy, Stéphane</au><au>Benoît, Gérard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Computer-assisted anatomic dissection (CAAD): evolution, methodology and application in intra-pelvic innervation study</atitle><jtitle>Surgical and radiologic anatomy (English ed.)</jtitle><stitle>Surg Radiol Anat</stitle><addtitle>Surg Radiol Anat</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>34</volume><issue>8</issue><spage>721</spage><epage>729</epage><pages>721-729</pages><issn>0930-1038</issn><eissn>1279-8517</eissn><abstract>Objective
Classic anatomical methods have limitations in micro determination of nerve fibre location. Furthermore, the precise detection of the nerve fibres nature is not possible by means of dissection. The combination of immunohistochemistry and three-dimensional reconstruction could be used to resolve these limitations of morphological sciences. Our aim is to describe the evolution of computer-assisted anatomic dissection (CAAD), which is an original method applied to study the distribution of intra-pelvic nerves in anatomic research.
Materials and methods
Serial transverse sectioning of the pelvic region in rabbit, human fetus, infant and adult cadaver was performed. Sections were immuno-histochemically stained and digitized with a high optical resolution scanner. Photoshop 7 software was used in regrouping of the adult cadaver sections then a tri-dimensional reconstruction was achieved using WinSurf software.
Results
The 3D reconstruction of the immuno-histochemically stained histologic sections of the pelvis allowed for precise structural identification of the prostate and its innervations (in fetus, infant and adult). In addition, we reconstructed the entire intra-pelvic organs with accurate demonstration of the location of both adrenergic and cholinergic pathways. Moreover, we performed a virtual dissection of each of the pelvic structures with description of the exact location of the inferior hypogastric plexus, as well as the nature and the distribution of its fibres.
Conclusion
The CAAD is an original method in anatomic research, which illustrates the fact that descriptive anatomy is still a dynamic science. This method allows for a 3D presentation of the intra-organic innervation, the nature of the nerve fibres, and the distribution of receptors and their neurotransmitters. This technique improves the understanding of the complex anatomic regions such as the pelvis from both surgical and educational point of view.</abstract><cop>Paris</cop><pub>Springer-Verlag</pub><pmid>21779928</pmid><doi>10.1007/s00276-011-0855-1</doi><tpages>9</tpages></addata></record> |
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| subjects | 3-D technology Adult Anatomy Anatomy & physiology Animals Cadaver Dissection Dissection - methods Fetus Humans Image Processing, Computer-Assisted - methods Imaging Imaging, Three-Dimensional - methods Infant Medicine Medicine & Public Health Nervous system Original Article Orthopedics Pelvis - innervation Rabbits Radiology Surgery |
| title | Computer-assisted anatomic dissection (CAAD): evolution, methodology and application in intra-pelvic innervation study |
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