Efficacy of platelet-rich plasma gel and hyaluronan hydrogel as carriers of electrically polarized hydroxyapatite microgranules for accelerating bone formation

The technology for electrical polarization and characterization of hydroxyapatite (HA) microgranules has been developed. This study aimed to examine and compare the efficacy of composites comprising electrically polarized HA (pHA) microgranules and platelet‐rich plasma (PRP) or hyaluronan (HAN) in o...

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Veröffentlicht in:Journal of biomedical materials research. Part A 2012-11, Vol.100A (11), p.3167-3176
Hauptverfasser: Ohba, Seiko, Wang, Wei, Itoh, Soichiro, Takagi, Yuzo, Nagai, Akiko, Yamashita, Kimihiro
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container_end_page 3176
container_issue 11
container_start_page 3167
container_title Journal of biomedical materials research. Part A
container_volume 100A
creator Ohba, Seiko
Wang, Wei
Itoh, Soichiro
Takagi, Yuzo
Nagai, Akiko
Yamashita, Kimihiro
description The technology for electrical polarization and characterization of hydroxyapatite (HA) microgranules has been developed. This study aimed to examine and compare the efficacy of composites comprising electrically polarized HA (pHA) microgranules and platelet‐rich plasma (PRP) or hyaluronan (HAN) in osteoconductivity. Composites of HA microgranules with or without electrical polarization and either PRP or HAN (PRP+pHA, PRP+HA, HAN+pHA, and HAN+HA, respectively), as well as pHA and HA microgranules were implanted randomly into holes created in the medial femoral condyle or tibial tuberosity of rabbits. As a control, PRP or HAN gel alone was implanted, or the bone holes were left empty. Each group included six animals. After 6 weeks, histological examination was performed, and osteoclastic and osteoblastic cell activities were assessed by cell counting. Although PRP alone could not induce bone formation, PRP+pHA and PRP+HA composites, especially the former, activated osteogenic cells and enhanced bone formation. This effect was not prominent in the HAN+pHA and HAN+HA composites. PRP+HA composites formed a gel in which the ceramic particles were dispersed and entrapped in the fibrin network of PRP. It is assumed that these particles provide scaffolds for osteogenic cells, and when electrically polarized, can activate the cells in co‐operation with the positive effects of the PRP, resulting in enhanced bone formation. Conversely, it is conceivable that this composite gel cannot act as an accelerator for woven bone formation, because HAN with low viscoelasticity is absorbed rapidly after implantation, the hydrated network containing HA microgranules is destroyed, and the HA microgranules effuse with HAN from the bone hole. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3167–3176, 2012.
doi_str_mv 10.1002/jbm.a.34250
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This study aimed to examine and compare the efficacy of composites comprising electrically polarized HA (pHA) microgranules and platelet‐rich plasma (PRP) or hyaluronan (HAN) in osteoconductivity. Composites of HA microgranules with or without electrical polarization and either PRP or HAN (PRP+pHA, PRP+HA, HAN+pHA, and HAN+HA, respectively), as well as pHA and HA microgranules were implanted randomly into holes created in the medial femoral condyle or tibial tuberosity of rabbits. As a control, PRP or HAN gel alone was implanted, or the bone holes were left empty. Each group included six animals. After 6 weeks, histological examination was performed, and osteoclastic and osteoblastic cell activities were assessed by cell counting. Although PRP alone could not induce bone formation, PRP+pHA and PRP+HA composites, especially the former, activated osteogenic cells and enhanced bone formation. This effect was not prominent in the HAN+pHA and HAN+HA composites. PRP+HA composites formed a gel in which the ceramic particles were dispersed and entrapped in the fibrin network of PRP. It is assumed that these particles provide scaffolds for osteogenic cells, and when electrically polarized, can activate the cells in co‐operation with the positive effects of the PRP, resulting in enhanced bone formation. Conversely, it is conceivable that this composite gel cannot act as an accelerator for woven bone formation, because HAN with low viscoelasticity is absorbed rapidly after implantation, the hydrated network containing HA microgranules is destroyed, and the HA microgranules effuse with HAN from the bone hole. © 2012 Wiley Periodicals, Inc. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>The technology for electrical polarization and characterization of hydroxyapatite (HA) microgranules has been developed. This study aimed to examine and compare the efficacy of composites comprising electrically polarized HA (pHA) microgranules and platelet‐rich plasma (PRP) or hyaluronan (HAN) in osteoconductivity. Composites of HA microgranules with or without electrical polarization and either PRP or HAN (PRP+pHA, PRP+HA, HAN+pHA, and HAN+HA, respectively), as well as pHA and HA microgranules were implanted randomly into holes created in the medial femoral condyle or tibial tuberosity of rabbits. As a control, PRP or HAN gel alone was implanted, or the bone holes were left empty. Each group included six animals. After 6 weeks, histological examination was performed, and osteoclastic and osteoblastic cell activities were assessed by cell counting. 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J Biomed Mater Res Part A: 100A:3167–3176, 2012.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Substitutes - chemistry</subject><subject>Bone Substitutes - metabolism</subject><subject>Bone Substitutes - therapeutic use</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - metabolism</subject><subject>Durapatite - therapeutic use</subject><subject>electrical polarization</subject><subject>Electrochemical Techniques</subject><subject>Femur - cytology</subject><subject>Femur - growth &amp; development</subject><subject>Femur - injuries</subject><subject>hyaluronan</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Hyaluronic Acid - metabolism</subject><subject>Hyaluronic Acid - therapeutic use</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - therapeutic use</subject><subject>hydroxyapatite</subject><subject>Medical sciences</subject><subject>osteoconductivity</subject><subject>Osteogenesis</subject><subject>platelet-rich plasma</subject><subject>Platelet-Rich Plasma - chemistry</subject><subject>Platelet-Rich Plasma - metabolism</subject><subject>Rabbits</subject><subject>Surgery (general aspects). 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohba, Seiko</au><au>Wang, Wei</au><au>Itoh, Soichiro</au><au>Takagi, Yuzo</au><au>Nagai, Akiko</au><au>Yamashita, Kimihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of platelet-rich plasma gel and hyaluronan hydrogel as carriers of electrically polarized hydroxyapatite microgranules for accelerating bone formation</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2012-11</date><risdate>2012</risdate><volume>100A</volume><issue>11</issue><spage>3167</spage><epage>3176</epage><pages>3167-3176</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>The technology for electrical polarization and characterization of hydroxyapatite (HA) microgranules has been developed. This study aimed to examine and compare the efficacy of composites comprising electrically polarized HA (pHA) microgranules and platelet‐rich plasma (PRP) or hyaluronan (HAN) in osteoconductivity. Composites of HA microgranules with or without electrical polarization and either PRP or HAN (PRP+pHA, PRP+HA, HAN+pHA, and HAN+HA, respectively), as well as pHA and HA microgranules were implanted randomly into holes created in the medial femoral condyle or tibial tuberosity of rabbits. As a control, PRP or HAN gel alone was implanted, or the bone holes were left empty. Each group included six animals. After 6 weeks, histological examination was performed, and osteoclastic and osteoblastic cell activities were assessed by cell counting. Although PRP alone could not induce bone formation, PRP+pHA and PRP+HA composites, especially the former, activated osteogenic cells and enhanced bone formation. This effect was not prominent in the HAN+pHA and HAN+HA composites. PRP+HA composites formed a gel in which the ceramic particles were dispersed and entrapped in the fibrin network of PRP. It is assumed that these particles provide scaffolds for osteogenic cells, and when electrically polarized, can activate the cells in co‐operation with the positive effects of the PRP, resulting in enhanced bone formation. Conversely, it is conceivable that this composite gel cannot act as an accelerator for woven bone formation, because HAN with low viscoelasticity is absorbed rapidly after implantation, the hydrated network containing HA microgranules is destroyed, and the HA microgranules effuse with HAN from the bone hole. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3167–3176, 2012.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22847859</pmid><doi>10.1002/jbm.a.34250</doi><tpages>10</tpages></addata></record>
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subjects Animals
Biological and medical sciences
Bone Substitutes - chemistry
Bone Substitutes - metabolism
Bone Substitutes - therapeutic use
Durapatite - chemistry
Durapatite - metabolism
Durapatite - therapeutic use
electrical polarization
Electrochemical Techniques
Femur - cytology
Femur - growth & development
Femur - injuries
hyaluronan
Hyaluronic Acid - chemistry
Hyaluronic Acid - metabolism
Hyaluronic Acid - therapeutic use
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism
Hydrogel, Polyethylene Glycol Dimethacrylate - therapeutic use
hydroxyapatite
Medical sciences
osteoconductivity
Osteogenesis
platelet-rich plasma
Platelet-Rich Plasma - chemistry
Platelet-Rich Plasma - metabolism
Rabbits
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Tibia - cytology
Tibia - growth & development
Tibia - injuries
title Efficacy of platelet-rich plasma gel and hyaluronan hydrogel as carriers of electrically polarized hydroxyapatite microgranules for accelerating bone formation
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