A short-term in vivo experimental model for Fuchs endothelial corneal dystrophy
We evaluated the in vivo functionality of a corneal endothelium tissue-engineered using corneal endothelial cells from human patients with Fuchs endothelial corneal dystrophy (FECD). A total of 15 healthy cats underwent full-thickness corneal transplantation. All transplants were of xenogeneic human...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2012-09, Vol.53 (10), p.6343-6354 |
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| creator | Haydari, M Nour Perron, Marie-Claude Laprise, Simon Roy, Olivier Cameron, J Douglas Proulx, Stéphanie Brunette, Isabelle |
| description | We evaluated the in vivo functionality of a corneal endothelium tissue-engineered using corneal endothelial cells from human patients with Fuchs endothelial corneal dystrophy (FECD).
A total of 15 healthy cats underwent full-thickness corneal transplantation. All transplants were of xenogeneic human origin and all grafts but two were tissue-engineered. In seven animals the graft corneal endothelium was tissue-engineered using cultured corneal endothelial cells from humans with FECD (TE-FECD). Two control animals were grafted with an endothelium engineered using cultured endothelial cells from normal eye bank corneas (TE-normal). Two controls received a native full-thickness corneal transplant, and four other controls were grafted with the stromal carrier only (without endothelial cells). Outcome parameters included graft transparency (0, opaque to 4, clear), pachymetry, optical coherence tomography, endothelial cell morphometry, transmission electron microscopy (TEM), and immunostaining of function-related proteins.
Seven days after transplantation, 6 of 7 TE-FECD grafts, all TE-normal grafts, and all normal native grafts were clear (transparency score >3), while all carriers-only grafts were opaque (score |
| doi_str_mv | 10.1167/iovs.12-9708 |
| format | Article |
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A total of 15 healthy cats underwent full-thickness corneal transplantation. All transplants were of xenogeneic human origin and all grafts but two were tissue-engineered. In seven animals the graft corneal endothelium was tissue-engineered using cultured corneal endothelial cells from humans with FECD (TE-FECD). Two control animals were grafted with an endothelium engineered using cultured endothelial cells from normal eye bank corneas (TE-normal). Two controls received a native full-thickness corneal transplant, and four other controls were grafted with the stromal carrier only (without endothelial cells). Outcome parameters included graft transparency (0, opaque to 4, clear), pachymetry, optical coherence tomography, endothelial cell morphometry, transmission electron microscopy (TEM), and immunostaining of function-related proteins.
Seven days after transplantation, 6 of 7 TE-FECD grafts, all TE-normal grafts, and all normal native grafts were clear (transparency score >3), while all carriers-only grafts were opaque (score <1). The mean pachymetry was 772 ± 102 μm for TE-FECD, 524 ± 11 μm for TE-normal, 555 ± 48 for normal native, and 1188 ± 223 μm for carriers only. TEM showed subendothelial loose fibrillar material deposition in all TE-FECD grafts. The TE endothelium expressed Na(+)-K(+)/ATPase and Na(+)/HCO3(-).
Restoration of transparency and corneal thickness demonstrated that the TE-FECD grafts were functional in vivo. This novel FECD seven-day living model suggests a potential role for tissue engineering leading to FECD cell rehabilitation.</description><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.12-9708</identifier><identifier>PMID: 22915029</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Animals ; Cats ; Cell Count ; Corneal Pachymetry ; Corneal Transplantation - methods ; Disease Models, Animal ; Endothelial Cells - physiology ; Endothelial Cells - ultrastructure ; Endothelium, Corneal - pathology ; Endothelium, Corneal - physiopathology ; Endothelium, Corneal - surgery ; Eye Banks ; Female ; Fuchs' Endothelial Dystrophy - pathology ; Fuchs' Endothelial Dystrophy - physiopathology ; Fuchs' Endothelial Dystrophy - surgery ; Humans ; Intraocular Pressure - physiology ; Male ; Microscopy, Electron, Transmission ; Middle Aged ; Postoperative Complications - diagnosis ; Primary Cell Culture ; Tissue Engineering - methods ; Transplantation, Heterologous</subject><ispartof>Investigative ophthalmology & visual science, 2012-09, Vol.53 (10), p.6343-6354</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c291t-7c58495c0ddf4f04cf7cedb761eaf8558de2b228bcf8f94c500cc92e5f1c11653</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22915029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haydari, M Nour</creatorcontrib><creatorcontrib>Perron, Marie-Claude</creatorcontrib><creatorcontrib>Laprise, Simon</creatorcontrib><creatorcontrib>Roy, Olivier</creatorcontrib><creatorcontrib>Cameron, J Douglas</creatorcontrib><creatorcontrib>Proulx, Stéphanie</creatorcontrib><creatorcontrib>Brunette, Isabelle</creatorcontrib><title>A short-term in vivo experimental model for Fuchs endothelial corneal dystrophy</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>We evaluated the in vivo functionality of a corneal endothelium tissue-engineered using corneal endothelial cells from human patients with Fuchs endothelial corneal dystrophy (FECD).
A total of 15 healthy cats underwent full-thickness corneal transplantation. All transplants were of xenogeneic human origin and all grafts but two were tissue-engineered. In seven animals the graft corneal endothelium was tissue-engineered using cultured corneal endothelial cells from humans with FECD (TE-FECD). Two control animals were grafted with an endothelium engineered using cultured endothelial cells from normal eye bank corneas (TE-normal). Two controls received a native full-thickness corneal transplant, and four other controls were grafted with the stromal carrier only (without endothelial cells). Outcome parameters included graft transparency (0, opaque to 4, clear), pachymetry, optical coherence tomography, endothelial cell morphometry, transmission electron microscopy (TEM), and immunostaining of function-related proteins.
Seven days after transplantation, 6 of 7 TE-FECD grafts, all TE-normal grafts, and all normal native grafts were clear (transparency score >3), while all carriers-only grafts were opaque (score <1). The mean pachymetry was 772 ± 102 μm for TE-FECD, 524 ± 11 μm for TE-normal, 555 ± 48 for normal native, and 1188 ± 223 μm for carriers only. TEM showed subendothelial loose fibrillar material deposition in all TE-FECD grafts. The TE endothelium expressed Na(+)-K(+)/ATPase and Na(+)/HCO3(-).
Restoration of transparency and corneal thickness demonstrated that the TE-FECD grafts were functional in vivo. This novel FECD seven-day living model suggests a potential role for tissue engineering leading to FECD cell rehabilitation.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Animals</subject><subject>Cats</subject><subject>Cell Count</subject><subject>Corneal Pachymetry</subject><subject>Corneal Transplantation - methods</subject><subject>Disease Models, Animal</subject><subject>Endothelial Cells - physiology</subject><subject>Endothelial Cells - ultrastructure</subject><subject>Endothelium, Corneal - pathology</subject><subject>Endothelium, Corneal - physiopathology</subject><subject>Endothelium, Corneal - surgery</subject><subject>Eye Banks</subject><subject>Female</subject><subject>Fuchs' Endothelial Dystrophy - pathology</subject><subject>Fuchs' Endothelial Dystrophy - physiopathology</subject><subject>Fuchs' Endothelial Dystrophy - surgery</subject><subject>Humans</subject><subject>Intraocular Pressure - physiology</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Middle Aged</subject><subject>Postoperative Complications - diagnosis</subject><subject>Primary Cell Culture</subject><subject>Tissue Engineering - methods</subject><subject>Transplantation, Heterologous</subject><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMFLwzAUh4Mobk5vniVHD3YmWdM2xzE2FQa76Dm0yQuttE1N0uH-ezM2xdPvwfv4Pd6H0D0lc0qz_Lmxez-nLBE5KS7QlHLOEp4Xi8t_8wTdeP9JCKOUkWs0YUxQTpiYot0S-9q6kARwHW56vG_2FsP3AK7poA9lizurocXGOrwZVe0x9NqGGtom7pR1PcTUBx-cHerDLboyZevh7pwz9LFZv69ek-3u5W213CYqng5JrniRCq6I1iY1JFUmV6CrPKNQmoLzQgOrGCsqZQojUsUJUUow4Iaq-DVfzNDjqXdw9msEH2TXeAVtW_ZgRy8pyQQjaZ5lEX06ocpZ7x0YOcTfSneIkDwqlEeFkjJ5VBjxh3PzWHWg_-BfZ4sfE1ZuPg</recordid><startdate>20120919</startdate><enddate>20120919</enddate><creator>Haydari, M Nour</creator><creator>Perron, Marie-Claude</creator><creator>Laprise, Simon</creator><creator>Roy, Olivier</creator><creator>Cameron, J Douglas</creator><creator>Proulx, Stéphanie</creator><creator>Brunette, Isabelle</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120919</creationdate><title>A short-term in vivo experimental model for Fuchs endothelial corneal dystrophy</title><author>Haydari, M Nour ; Perron, Marie-Claude ; Laprise, Simon ; Roy, Olivier ; Cameron, J Douglas ; Proulx, Stéphanie ; Brunette, Isabelle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c291t-7c58495c0ddf4f04cf7cedb761eaf8558de2b228bcf8f94c500cc92e5f1c11653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Animals</topic><topic>Cats</topic><topic>Cell Count</topic><topic>Corneal Pachymetry</topic><topic>Corneal Transplantation - methods</topic><topic>Disease Models, Animal</topic><topic>Endothelial Cells - physiology</topic><topic>Endothelial Cells - ultrastructure</topic><topic>Endothelium, Corneal - pathology</topic><topic>Endothelium, Corneal - physiopathology</topic><topic>Endothelium, Corneal - surgery</topic><topic>Eye Banks</topic><topic>Female</topic><topic>Fuchs' Endothelial Dystrophy - pathology</topic><topic>Fuchs' Endothelial Dystrophy - physiopathology</topic><topic>Fuchs' Endothelial Dystrophy - surgery</topic><topic>Humans</topic><topic>Intraocular Pressure - physiology</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>Middle Aged</topic><topic>Postoperative Complications - diagnosis</topic><topic>Primary Cell Culture</topic><topic>Tissue Engineering - methods</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haydari, M Nour</creatorcontrib><creatorcontrib>Perron, Marie-Claude</creatorcontrib><creatorcontrib>Laprise, Simon</creatorcontrib><creatorcontrib>Roy, Olivier</creatorcontrib><creatorcontrib>Cameron, J Douglas</creatorcontrib><creatorcontrib>Proulx, Stéphanie</creatorcontrib><creatorcontrib>Brunette, Isabelle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haydari, M Nour</au><au>Perron, Marie-Claude</au><au>Laprise, Simon</au><au>Roy, Olivier</au><au>Cameron, J Douglas</au><au>Proulx, Stéphanie</au><au>Brunette, Isabelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A short-term in vivo experimental model for Fuchs endothelial corneal dystrophy</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2012-09-19</date><risdate>2012</risdate><volume>53</volume><issue>10</issue><spage>6343</spage><epage>6354</epage><pages>6343-6354</pages><issn>1552-5783</issn><eissn>1552-5783</eissn><abstract>We evaluated the in vivo functionality of a corneal endothelium tissue-engineered using corneal endothelial cells from human patients with Fuchs endothelial corneal dystrophy (FECD).
A total of 15 healthy cats underwent full-thickness corneal transplantation. All transplants were of xenogeneic human origin and all grafts but two were tissue-engineered. In seven animals the graft corneal endothelium was tissue-engineered using cultured corneal endothelial cells from humans with FECD (TE-FECD). Two control animals were grafted with an endothelium engineered using cultured endothelial cells from normal eye bank corneas (TE-normal). Two controls received a native full-thickness corneal transplant, and four other controls were grafted with the stromal carrier only (without endothelial cells). Outcome parameters included graft transparency (0, opaque to 4, clear), pachymetry, optical coherence tomography, endothelial cell morphometry, transmission electron microscopy (TEM), and immunostaining of function-related proteins.
Seven days after transplantation, 6 of 7 TE-FECD grafts, all TE-normal grafts, and all normal native grafts were clear (transparency score >3), while all carriers-only grafts were opaque (score <1). The mean pachymetry was 772 ± 102 μm for TE-FECD, 524 ± 11 μm for TE-normal, 555 ± 48 for normal native, and 1188 ± 223 μm for carriers only. TEM showed subendothelial loose fibrillar material deposition in all TE-FECD grafts. The TE endothelium expressed Na(+)-K(+)/ATPase and Na(+)/HCO3(-).
Restoration of transparency and corneal thickness demonstrated that the TE-FECD grafts were functional in vivo. This novel FECD seven-day living model suggests a potential role for tissue engineering leading to FECD cell rehabilitation.</abstract><cop>United States</cop><pmid>22915029</pmid><doi>10.1167/iovs.12-9708</doi><tpages>12</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Animals Cats Cell Count Corneal Pachymetry Corneal Transplantation - methods Disease Models, Animal Endothelial Cells - physiology Endothelial Cells - ultrastructure Endothelium, Corneal - pathology Endothelium, Corneal - physiopathology Endothelium, Corneal - surgery Eye Banks Female Fuchs' Endothelial Dystrophy - pathology Fuchs' Endothelial Dystrophy - physiopathology Fuchs' Endothelial Dystrophy - surgery Humans Intraocular Pressure - physiology Male Microscopy, Electron, Transmission Middle Aged Postoperative Complications - diagnosis Primary Cell Culture Tissue Engineering - methods Transplantation, Heterologous |
| title | A short-term in vivo experimental model for Fuchs endothelial corneal dystrophy |
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