Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein
Yellow catfish (Pelteobagrus fulvidraco Richardson) is one of the most important freshwater farmed species in China. However, its small size and slow growth rate limit its commercial value. Because genetic engineering has been a powerful tool to develop and improve fish traits for aquaculture, we pe...
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| Veröffentlicht in: | Transgenic research 2012-10, Vol.21 (5), p.995-1004 |
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| creator | Ge, Jiachun Dong, Zhangji Li, Jingyun Xu, Zhiqiang Song, Wei Bao, Jie Liang, Dong Li, Junbo Li, Kui Jia, Wenshuang Zhao, Muzi Cai, Yongxiang Yang, Jiaxin Pan, Jianlin Zhao, Qingshun |
| description | Yellow catfish (Pelteobagrus fulvidraco Richardson) is one of the most important freshwater farmed species in China. However, its small size and slow growth rate limit its commercial value. Because genetic engineering has been a powerful tool to develop and improve fish traits for aquaculture, we performed transgenic research on yellow catfish in order to increase its size and growth rate. Performing PCR with degenerate primers, we cloned a genomic fragment comprising 5′-flanking sequence upstream of the initiation codon of β-actin gene in yellow catfish. The sequence is 1,017 bp long, containing the core sequence of proximal promoter including CAAT box, CArG motif and TATA box. Microinjecting the transgene construct Tg(beta-actin:eYFP) of the proximal promoter fused to enhanced yellow fluorescent protein (eYFP) reporter gene into zebrafish and yellow catfish embryos, we found the promoter could drive the reporter to express transiently in both embryos at early development. Screening the offspring of five transgenic zebrafish founders developed from the embryos microinjected with Tg(ycbeta-actin:mCherry) or 19 yellow catfish founders developed from the embryos microinjected with Tg(beta-actin:eYFP), we obtained three lines of transgenic zebrafish and one transgenic yellow catfish, respectively. Analyzing the expression patterns of the reporter genes in transgenic zebrafish (Tg(ycbeta-actin:mCherry)nju8/+) and transgenic yellow catfish (Tg(beta-actin:eYFP)nju11/+), we found the reporters were broadly expressed in both animals. In summary, we have established a platform to make transgenic yellow catfish using the proximal promoter of its own β-actin gene. The results will help us to create transgenic yellow catfish using “all yellow catfish” transgene constructs. |
| doi_str_mv | 10.1007/s11248-012-9606-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1069203662</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1069203662</sourcerecordid><originalsourceid>FETCH-LOGICAL-c431t-9f8cd30591b146c5b3709916c51b587dc09abdc135879c878595e670dfa822b63</originalsourceid><addsrcrecordid>eNqNkcFuFSEUhonR2OvVB3Cjs2niBj3ADANL01Rt0sSFdk0YBm5p5sIVmNS-gc_jg_hMMpnbmrgwrg5wvv8_5PwIvSTwlgD07zIhtBUYCMWSA8f0EdqQrmdYMi4eow1ITrEQRJ6gZznfAFSVYE_RCaUt9C3wDfpxkeOki4-hia65s9MUbxuji_P5uvn1E2tTfGgOKe5jsanRYWx2Ntj0IClJh1yfvPlbbb8fks3Zh11jw7UOxo73iJvmWHvGhrJ4F-vDc_TE6SnbF8e6RVcfzr-efcKXnz9enL2_xKZlpGDphBkZdJIMpOWmG1gPUpJ6IkMn-tGA1MNoCKsXaUQvOtlZ3sPotKB04GyL3qy-de632eai9r5-ZJp0sHHOigCXFBjn9D9QJqCntNYtIitqUsw5WacOye91uquQWrJSa1aqZqWWrNRi_-poPw97Oz4o7sOpwOkR0NnoydVNG5__cJyBkHQxoiuXayvsbFI3cU6hbvGf01-vIqej0rtUja--UCAtAPC-6zr2G1zwt7A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1038072210</pqid></control><display><type>article</type><title>Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Ge, Jiachun ; Dong, Zhangji ; Li, Jingyun ; Xu, Zhiqiang ; Song, Wei ; Bao, Jie ; Liang, Dong ; Li, Junbo ; Li, Kui ; Jia, Wenshuang ; Zhao, Muzi ; Cai, Yongxiang ; Yang, Jiaxin ; Pan, Jianlin ; Zhao, Qingshun</creator><creatorcontrib>Ge, Jiachun ; Dong, Zhangji ; Li, Jingyun ; Xu, Zhiqiang ; Song, Wei ; Bao, Jie ; Liang, Dong ; Li, Junbo ; Li, Kui ; Jia, Wenshuang ; Zhao, Muzi ; Cai, Yongxiang ; Yang, Jiaxin ; Pan, Jianlin ; Zhao, Qingshun</creatorcontrib><description>Yellow catfish (Pelteobagrus fulvidraco Richardson) is one of the most important freshwater farmed species in China. However, its small size and slow growth rate limit its commercial value. Because genetic engineering has been a powerful tool to develop and improve fish traits for aquaculture, we performed transgenic research on yellow catfish in order to increase its size and growth rate. Performing PCR with degenerate primers, we cloned a genomic fragment comprising 5′-flanking sequence upstream of the initiation codon of β-actin gene in yellow catfish. The sequence is 1,017 bp long, containing the core sequence of proximal promoter including CAAT box, CArG motif and TATA box. Microinjecting the transgene construct Tg(beta-actin:eYFP) of the proximal promoter fused to enhanced yellow fluorescent protein (eYFP) reporter gene into zebrafish and yellow catfish embryos, we found the promoter could drive the reporter to express transiently in both embryos at early development. Screening the offspring of five transgenic zebrafish founders developed from the embryos microinjected with Tg(ycbeta-actin:mCherry) or 19 yellow catfish founders developed from the embryos microinjected with Tg(beta-actin:eYFP), we obtained three lines of transgenic zebrafish and one transgenic yellow catfish, respectively. Analyzing the expression patterns of the reporter genes in transgenic zebrafish (Tg(ycbeta-actin:mCherry)nju8/+) and transgenic yellow catfish (Tg(beta-actin:eYFP)nju11/+), we found the reporters were broadly expressed in both animals. In summary, we have established a platform to make transgenic yellow catfish using the proximal promoter of its own β-actin gene. The results will help us to create transgenic yellow catfish using “all yellow catfish” transgene constructs.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-012-9606-2</identifier><identifier>PMID: 22407406</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Actin ; Actins - genetics ; Actins - metabolism ; Animal Genetics and Genomics ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - metabolism ; Aquaculture ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biotechnology ; Body Size ; Catfishes - genetics ; Catfishes - metabolism ; Cloning, Molecular ; Codon, Initiator - genetics ; Codon, Initiator - metabolism ; Codons ; Danio rerio ; early development ; Embryo, Nonmammalian - cytology ; Embryo, Nonmammalian - metabolism ; Embryonic Development ; Embryos ; fish ; Freshwater environments ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Genes, Reporter ; Genetic Engineering ; Genetic Engineering - methods ; Genetic technics ; genomics ; Growth rate ; Life Sciences ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Methods. Procedures. Technologies ; Microinjections ; Molecular Medicine ; Original Paper ; Pelteobagrus fulvidraco ; Plant Genetics and Genomics ; Polymerase chain reaction ; Primers ; Progeny ; Promoter Regions, Genetic ; Promoters ; Reporter gene ; reporter genes ; screening ; start codon ; Tachysurus fulvidraco ; TATA box ; Transgenes ; Transgenic animals and transgenic plants ; Transgenics ; yellow fluorescent protein ; Zebrafish - genetics ; Zebrafish - metabolism</subject><ispartof>Transgenic research, 2012-10, Vol.21 (5), p.995-1004</ispartof><rights>Springer Science+Business Media B.V. 2012</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-9f8cd30591b146c5b3709916c51b587dc09abdc135879c878595e670dfa822b63</citedby><cites>FETCH-LOGICAL-c431t-9f8cd30591b146c5b3709916c51b587dc09abdc135879c878595e670dfa822b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11248-012-9606-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11248-012-9606-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26308922$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22407406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Jiachun</creatorcontrib><creatorcontrib>Dong, Zhangji</creatorcontrib><creatorcontrib>Li, Jingyun</creatorcontrib><creatorcontrib>Xu, Zhiqiang</creatorcontrib><creatorcontrib>Song, Wei</creatorcontrib><creatorcontrib>Bao, Jie</creatorcontrib><creatorcontrib>Liang, Dong</creatorcontrib><creatorcontrib>Li, Junbo</creatorcontrib><creatorcontrib>Li, Kui</creatorcontrib><creatorcontrib>Jia, Wenshuang</creatorcontrib><creatorcontrib>Zhao, Muzi</creatorcontrib><creatorcontrib>Cai, Yongxiang</creatorcontrib><creatorcontrib>Yang, Jiaxin</creatorcontrib><creatorcontrib>Pan, Jianlin</creatorcontrib><creatorcontrib>Zhao, Qingshun</creatorcontrib><title>Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><addtitle>Transgenic Res</addtitle><description>Yellow catfish (Pelteobagrus fulvidraco Richardson) is one of the most important freshwater farmed species in China. However, its small size and slow growth rate limit its commercial value. Because genetic engineering has been a powerful tool to develop and improve fish traits for aquaculture, we performed transgenic research on yellow catfish in order to increase its size and growth rate. Performing PCR with degenerate primers, we cloned a genomic fragment comprising 5′-flanking sequence upstream of the initiation codon of β-actin gene in yellow catfish. The sequence is 1,017 bp long, containing the core sequence of proximal promoter including CAAT box, CArG motif and TATA box. Microinjecting the transgene construct Tg(beta-actin:eYFP) of the proximal promoter fused to enhanced yellow fluorescent protein (eYFP) reporter gene into zebrafish and yellow catfish embryos, we found the promoter could drive the reporter to express transiently in both embryos at early development. Screening the offspring of five transgenic zebrafish founders developed from the embryos microinjected with Tg(ycbeta-actin:mCherry) or 19 yellow catfish founders developed from the embryos microinjected with Tg(beta-actin:eYFP), we obtained three lines of transgenic zebrafish and one transgenic yellow catfish, respectively. Analyzing the expression patterns of the reporter genes in transgenic zebrafish (Tg(ycbeta-actin:mCherry)nju8/+) and transgenic yellow catfish (Tg(beta-actin:eYFP)nju11/+), we found the reporters were broadly expressed in both animals. In summary, we have established a platform to make transgenic yellow catfish using the proximal promoter of its own β-actin gene. The results will help us to create transgenic yellow catfish using “all yellow catfish” transgene constructs.</description><subject>Actin</subject><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Animals, Genetically Modified - genetics</subject><subject>Animals, Genetically Modified - metabolism</subject><subject>Aquaculture</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biotechnology</subject><subject>Body Size</subject><subject>Catfishes - genetics</subject><subject>Catfishes - metabolism</subject><subject>Cloning, Molecular</subject><subject>Codon, Initiator - genetics</subject><subject>Codon, Initiator - metabolism</subject><subject>Codons</subject><subject>Danio rerio</subject><subject>early development</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Embryonic Development</subject><subject>Embryos</subject><subject>fish</subject><subject>Freshwater environments</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes, Reporter</subject><subject>Genetic Engineering</subject><subject>Genetic Engineering - methods</subject><subject>Genetic technics</subject><subject>genomics</subject><subject>Growth rate</subject><subject>Life Sciences</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Microinjections</subject><subject>Molecular Medicine</subject><subject>Original Paper</subject><subject>Pelteobagrus fulvidraco</subject><subject>Plant Genetics and Genomics</subject><subject>Polymerase chain reaction</subject><subject>Primers</subject><subject>Progeny</subject><subject>Promoter Regions, Genetic</subject><subject>Promoters</subject><subject>Reporter gene</subject><subject>reporter genes</subject><subject>screening</subject><subject>start codon</subject><subject>Tachysurus fulvidraco</subject><subject>TATA box</subject><subject>Transgenes</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenics</subject><subject>yellow fluorescent protein</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish - metabolism</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFuFSEUhonR2OvVB3Cjs2niBj3ADANL01Rt0sSFdk0YBm5p5sIVmNS-gc_jg_hMMpnbmrgwrg5wvv8_5PwIvSTwlgD07zIhtBUYCMWSA8f0EdqQrmdYMi4eow1ITrEQRJ6gZznfAFSVYE_RCaUt9C3wDfpxkeOki4-hia65s9MUbxuji_P5uvn1E2tTfGgOKe5jsanRYWx2Ntj0IClJh1yfvPlbbb8fks3Zh11jw7UOxo73iJvmWHvGhrJ4F-vDc_TE6SnbF8e6RVcfzr-efcKXnz9enL2_xKZlpGDphBkZdJIMpOWmG1gPUpJ6IkMn-tGA1MNoCKsXaUQvOtlZ3sPotKB04GyL3qy-de632eai9r5-ZJp0sHHOigCXFBjn9D9QJqCntNYtIitqUsw5WacOye91uquQWrJSa1aqZqWWrNRi_-poPw97Oz4o7sOpwOkR0NnoydVNG5__cJyBkHQxoiuXayvsbFI3cU6hbvGf01-vIqej0rtUja--UCAtAPC-6zr2G1zwt7A</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Ge, Jiachun</creator><creator>Dong, Zhangji</creator><creator>Li, Jingyun</creator><creator>Xu, Zhiqiang</creator><creator>Song, Wei</creator><creator>Bao, Jie</creator><creator>Liang, Dong</creator><creator>Li, Junbo</creator><creator>Li, Kui</creator><creator>Jia, Wenshuang</creator><creator>Zhao, Muzi</creator><creator>Cai, Yongxiang</creator><creator>Yang, Jiaxin</creator><creator>Pan, Jianlin</creator><creator>Zhao, Qingshun</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20121001</creationdate><title>Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein</title><author>Ge, Jiachun ; Dong, Zhangji ; Li, Jingyun ; Xu, Zhiqiang ; Song, Wei ; Bao, Jie ; Liang, Dong ; Li, Junbo ; Li, Kui ; Jia, Wenshuang ; Zhao, Muzi ; Cai, Yongxiang ; Yang, Jiaxin ; Pan, Jianlin ; Zhao, Qingshun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-9f8cd30591b146c5b3709916c51b587dc09abdc135879c878595e670dfa822b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Actin</topic><topic>Actins - genetics</topic><topic>Actins - metabolism</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Animals, Genetically Modified - genetics</topic><topic>Animals, Genetically Modified - metabolism</topic><topic>Aquaculture</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biotechnology</topic><topic>Body Size</topic><topic>Catfishes - genetics</topic><topic>Catfishes - metabolism</topic><topic>Cloning, Molecular</topic><topic>Codon, Initiator - genetics</topic><topic>Codon, Initiator - metabolism</topic><topic>Codons</topic><topic>Danio rerio</topic><topic>early development</topic><topic>Embryo, Nonmammalian - cytology</topic><topic>Embryo, Nonmammalian - metabolism</topic><topic>Embryonic Development</topic><topic>Embryos</topic><topic>fish</topic><topic>Freshwater environments</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes, Reporter</topic><topic>Genetic Engineering</topic><topic>Genetic Engineering - methods</topic><topic>Genetic technics</topic><topic>genomics</topic><topic>Growth rate</topic><topic>Life Sciences</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Microinjections</topic><topic>Molecular Medicine</topic><topic>Original Paper</topic><topic>Pelteobagrus fulvidraco</topic><topic>Plant Genetics and Genomics</topic><topic>Polymerase chain reaction</topic><topic>Primers</topic><topic>Progeny</topic><topic>Promoter Regions, Genetic</topic><topic>Promoters</topic><topic>Reporter gene</topic><topic>reporter genes</topic><topic>screening</topic><topic>start codon</topic><topic>Tachysurus fulvidraco</topic><topic>TATA box</topic><topic>Transgenes</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenics</topic><topic>yellow fluorescent protein</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Jiachun</creatorcontrib><creatorcontrib>Dong, Zhangji</creatorcontrib><creatorcontrib>Li, Jingyun</creatorcontrib><creatorcontrib>Xu, Zhiqiang</creatorcontrib><creatorcontrib>Song, Wei</creatorcontrib><creatorcontrib>Bao, Jie</creatorcontrib><creatorcontrib>Liang, Dong</creatorcontrib><creatorcontrib>Li, Junbo</creatorcontrib><creatorcontrib>Li, Kui</creatorcontrib><creatorcontrib>Jia, Wenshuang</creatorcontrib><creatorcontrib>Zhao, Muzi</creatorcontrib><creatorcontrib>Cai, Yongxiang</creatorcontrib><creatorcontrib>Yang, Jiaxin</creatorcontrib><creatorcontrib>Pan, Jianlin</creatorcontrib><creatorcontrib>Zhao, Qingshun</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Jiachun</au><au>Dong, Zhangji</au><au>Li, Jingyun</au><au>Xu, Zhiqiang</au><au>Song, Wei</au><au>Bao, Jie</au><au>Liang, Dong</au><au>Li, Junbo</au><au>Li, Kui</au><au>Jia, Wenshuang</au><au>Zhao, Muzi</au><au>Cai, Yongxiang</au><au>Yang, Jiaxin</au><au>Pan, Jianlin</au><au>Zhao, Qingshun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein</atitle><jtitle>Transgenic research</jtitle><stitle>Transgenic Res</stitle><addtitle>Transgenic Res</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>21</volume><issue>5</issue><spage>995</spage><epage>1004</epage><pages>995-1004</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Yellow catfish (Pelteobagrus fulvidraco Richardson) is one of the most important freshwater farmed species in China. However, its small size and slow growth rate limit its commercial value. Because genetic engineering has been a powerful tool to develop and improve fish traits for aquaculture, we performed transgenic research on yellow catfish in order to increase its size and growth rate. Performing PCR with degenerate primers, we cloned a genomic fragment comprising 5′-flanking sequence upstream of the initiation codon of β-actin gene in yellow catfish. The sequence is 1,017 bp long, containing the core sequence of proximal promoter including CAAT box, CArG motif and TATA box. Microinjecting the transgene construct Tg(beta-actin:eYFP) of the proximal promoter fused to enhanced yellow fluorescent protein (eYFP) reporter gene into zebrafish and yellow catfish embryos, we found the promoter could drive the reporter to express transiently in both embryos at early development. Screening the offspring of five transgenic zebrafish founders developed from the embryos microinjected with Tg(ycbeta-actin:mCherry) or 19 yellow catfish founders developed from the embryos microinjected with Tg(beta-actin:eYFP), we obtained three lines of transgenic zebrafish and one transgenic yellow catfish, respectively. Analyzing the expression patterns of the reporter genes in transgenic zebrafish (Tg(ycbeta-actin:mCherry)nju8/+) and transgenic yellow catfish (Tg(beta-actin:eYFP)nju11/+), we found the reporters were broadly expressed in both animals. In summary, we have established a platform to make transgenic yellow catfish using the proximal promoter of its own β-actin gene. The results will help us to create transgenic yellow catfish using “all yellow catfish” transgene constructs.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>22407406</pmid><doi>10.1007/s11248-012-9606-2</doi><tpages>10</tpages></addata></record> |
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| subjects | Actin Actins - genetics Actins - metabolism Animal Genetics and Genomics Animals Animals, Genetically Modified - genetics Animals, Genetically Modified - metabolism Aquaculture Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biotechnology Body Size Catfishes - genetics Catfishes - metabolism Cloning, Molecular Codon, Initiator - genetics Codon, Initiator - metabolism Codons Danio rerio early development Embryo, Nonmammalian - cytology Embryo, Nonmammalian - metabolism Embryonic Development Embryos fish Freshwater environments Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Genes, Reporter Genetic Engineering Genetic Engineering - methods Genetic technics genomics Growth rate Life Sciences Luminescent Proteins - genetics Luminescent Proteins - metabolism Methods. Procedures. Technologies Microinjections Molecular Medicine Original Paper Pelteobagrus fulvidraco Plant Genetics and Genomics Polymerase chain reaction Primers Progeny Promoter Regions, Genetic Promoters Reporter gene reporter genes screening start codon Tachysurus fulvidraco TATA box Transgenes Transgenic animals and transgenic plants Transgenics yellow fluorescent protein Zebrafish - genetics Zebrafish - metabolism |
| title | Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein |
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