Chitin and L(+)-lactic acid production from crab (Callinectes bellicosus) wastes by fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source

Crab wastes are employed for simultaneous production of chitin and L(+)-lactic acid by submerged fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source. Response surface methodology was applied to design the culture media considering demineralization. Fermentations in stirre...

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Veröffentlicht in:Bioprocess and biosystems engineering 2012-09, Vol.35 (7), p.1193-1200
Hauptverfasser: Flores-Albino, Belem, Arias, Ladislao, Gómez, Jorge, Castillo, Alberto, Gimeno, Miquel, Shirai, Keiko
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container_end_page 1200
container_issue 7
container_start_page 1193
container_title Bioprocess and biosystems engineering
container_volume 35
creator Flores-Albino, Belem
Arias, Ladislao
Gómez, Jorge
Castillo, Alberto
Gimeno, Miquel
Shirai, Keiko
description Crab wastes are employed for simultaneous production of chitin and L(+)-lactic acid by submerged fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source. Response surface methodology was applied to design the culture media considering demineralization. Fermentations in stirred tank reactor (2L) using selected conditions produced 88% demineralization and 56% deproteinization with 34% yield of chitin and 19.5 gL−1 of lactic acid (77% yield). The chitin purified from fermentation displayed 95% degree of acetylation and 0.81 and 1 ± 0.125% of residual ash and protein contents, respectively.
doi_str_mv 10.1007/s00449-012-0706-4
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Psychology ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; lactic acid ; Lactic Acid - biosynthesis ; Lactobacillus ; Lactobacillus - metabolism ; Methods. Procedures. Technologies ; Microbial engineering. 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ispartof Bioprocess and biosystems engineering, 2012-09, Vol.35 (7), p.1193-1200
issn 1615-7591
1615-7605
language eng
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subjects acetylation
Acid production
Animals
ash (inorganic matter)
ash content
Bioengineering
Biological and medical sciences
bioprocessing
Bioreactors
Biotechnology
Callinectes
Callinectes bellicosus
Carbon
Carbon - metabolism
Carbon sources
Chemistry
Chemistry and Materials Science
Chitin
Chitin - biosynthesis
crabs
Crustacea - metabolism
Crustaceans
Culture Media
Decapoda
Demineralization
engineering
Environmental Engineering/Biotechnology
Fermentation
food processing wastes
Food Science
Fundamental and applied biological sciences. Psychology
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
lactic acid
Lactic Acid - biosynthesis
Lactobacillus
Lactobacillus - metabolism
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Molasses
Original Paper
protein content
proteins
response surface methodology
Saccharum - chemistry
Spectroscopy, Fourier Transform Infrared
submerged fermentation
Sugar
Sugarcane
tanks
Waste materials
title Chitin and L(+)-lactic acid production from crab (Callinectes bellicosus) wastes by fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source
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