Molecular cloning, characterization, and expression analysis of the Muscovy duck Toll-like receptor 3 (MdTLR3) gene
ABSTRACT Toll-like receptor 3 (TLR3) is an important membrane-bound receptor for recognizing double-stranded RNA in innate immunity. In this study, we described the cloning and characterization of the Muscovy duck TLR3 (MdTLR3) gene. The full-length MdTLR3 cDNA (2,836 bp) encoded a polypeptide of 89...
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| Veröffentlicht in: | Poultry science 2012-10, Vol.91 (10), p.2475-2481 |
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| container_title | Poultry science |
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| creator | Jiao, P. R. Wei, L. M. Cheng, Y. Q. Yuan, R. Y. Han, F. Liang, J. Liu, W. L. Ren, T. Xin, C. A. Liao, M. |
| description | ABSTRACT
Toll-like receptor 3 (TLR3) is an important membrane-bound receptor for recognizing double-stranded RNA in innate immunity. In this study, we described the cloning and characterization of the Muscovy duck TLR3 (MdTLR3) gene. The full-length MdTLR3 cDNA (2,836 bp) encoded a polypeptide of 895 amino acids. The deduced amino acid sequence contained 4 main structural domains: a signal peptide, an extracellular leucine rich repeats domain, a transmembrane domain, and a Toll/IL-1 receptor domain. Quantitative real-time PCR analysis indicated that MdTLR3 mRNA was constitutively expressed in all sampled tissues of uninfected Muscovy duck except muscle. Expression of MdTLR3 in brain was significantly upregulated at 24 h (1.94-fold, P < 0.05), reached a peak at 48 h (4.64-fold, P < 0.05), and recovered to normal levels at 72 h postinfection with the H5N1 highly pathogenic avian influenza virus. In contrast, MdTLR3 expression was downregulated during the test period in spleen and lung. These results implicated MdTLR3 was a novel member of the TLR family, which is involved in the early stage of antiviral innate immunity. |
| doi_str_mv | 10.3382/ps.2012-02394 |
| format | Article |
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Toll-like receptor 3 (TLR3) is an important membrane-bound receptor for recognizing double-stranded RNA in innate immunity. In this study, we described the cloning and characterization of the Muscovy duck TLR3 (MdTLR3) gene. The full-length MdTLR3 cDNA (2,836 bp) encoded a polypeptide of 895 amino acids. The deduced amino acid sequence contained 4 main structural domains: a signal peptide, an extracellular leucine rich repeats domain, a transmembrane domain, and a Toll/IL-1 receptor domain. Quantitative real-time PCR analysis indicated that MdTLR3 mRNA was constitutively expressed in all sampled tissues of uninfected Muscovy duck except muscle. Expression of MdTLR3 in brain was significantly upregulated at 24 h (1.94-fold, P < 0.05), reached a peak at 48 h (4.64-fold, P < 0.05), and recovered to normal levels at 72 h postinfection with the H5N1 highly pathogenic avian influenza virus. In contrast, MdTLR3 expression was downregulated during the test period in spleen and lung. These results implicated MdTLR3 was a novel member of the TLR family, which is involved in the early stage of antiviral innate immunity.</description><identifier>ISSN: 0032-5791</identifier><identifier>EISSN: 1525-3171</identifier><identifier>DOI: 10.3382/ps.2012-02394</identifier><identifier>PMID: 22991530</identifier><language>eng</language><publisher>Oxford, UK: Oxford University Press</publisher><subject>Amino Acid Sequence ; Animals ; Cloning, Molecular ; Ducks - genetics ; Ducks - metabolism ; Gene Expression Regulation - physiology ; Influenza A Virus, H5N1 Subtype ; Influenza in Birds - metabolism ; Influenza in Birds - virology ; Molecular Sequence Data ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Toll-Like Receptor 3 - genetics ; Toll-Like Receptor 3 - metabolism</subject><ispartof>Poultry science, 2012-10, Vol.91 (10), p.2475-2481</ispartof><rights>2012 Poultry Science Association Inc. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-630a96758b402d4b1157d358ab08dd0e24c1b9bf3e07d8616fd70ad39540814b3</citedby><cites>FETCH-LOGICAL-c365t-630a96758b402d4b1157d358ab08dd0e24c1b9bf3e07d8616fd70ad39540814b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22991530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiao, P. R.</creatorcontrib><creatorcontrib>Wei, L. M.</creatorcontrib><creatorcontrib>Cheng, Y. Q.</creatorcontrib><creatorcontrib>Yuan, R. Y.</creatorcontrib><creatorcontrib>Han, F.</creatorcontrib><creatorcontrib>Liang, J.</creatorcontrib><creatorcontrib>Liu, W. L.</creatorcontrib><creatorcontrib>Ren, T.</creatorcontrib><creatorcontrib>Xin, C. A.</creatorcontrib><creatorcontrib>Liao, M.</creatorcontrib><title>Molecular cloning, characterization, and expression analysis of the Muscovy duck Toll-like receptor 3 (MdTLR3) gene</title><title>Poultry science</title><addtitle>Poult Sci</addtitle><description>ABSTRACT
Toll-like receptor 3 (TLR3) is an important membrane-bound receptor for recognizing double-stranded RNA in innate immunity. In this study, we described the cloning and characterization of the Muscovy duck TLR3 (MdTLR3) gene. The full-length MdTLR3 cDNA (2,836 bp) encoded a polypeptide of 895 amino acids. The deduced amino acid sequence contained 4 main structural domains: a signal peptide, an extracellular leucine rich repeats domain, a transmembrane domain, and a Toll/IL-1 receptor domain. Quantitative real-time PCR analysis indicated that MdTLR3 mRNA was constitutively expressed in all sampled tissues of uninfected Muscovy duck except muscle. Expression of MdTLR3 in brain was significantly upregulated at 24 h (1.94-fold, P < 0.05), reached a peak at 48 h (4.64-fold, P < 0.05), and recovered to normal levels at 72 h postinfection with the H5N1 highly pathogenic avian influenza virus. In contrast, MdTLR3 expression was downregulated during the test period in spleen and lung. These results implicated MdTLR3 was a novel member of the TLR family, which is involved in the early stage of antiviral innate immunity.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cloning, Molecular</subject><subject>Ducks - genetics</subject><subject>Ducks - metabolism</subject><subject>Gene Expression Regulation - physiology</subject><subject>Influenza A Virus, H5N1 Subtype</subject><subject>Influenza in Birds - metabolism</subject><subject>Influenza in Birds - virology</subject><subject>Molecular Sequence Data</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Toll-Like Receptor 3 - genetics</subject><subject>Toll-Like Receptor 3 - metabolism</subject><issn>0032-5791</issn><issn>1525-3171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1LwzAYxoMoOqdHr5Kjwjrz0aTtUYZfsCHIPJc0ebtVs6YmrTj_ejs39eTp5YEfzwO_F6EzSsacp-yqCWNGKIsI41m8hwZUMBFxmtB9NCCEs0gkGT1CxyG8EMKolMkhOmIsy6jgZIDCzFnQnVUea-vqql6MsF4qr3QLvvpUbeXqEVa1wfDReAihz31Udh2qgF2J2yXgWRe0e19j0-lXPHfWRrZ6BexBQ9M6jzm-mJn59Ilf4gXUcIIOSmUDnO7uED3f3swn99H08e5hcj2NNJeijSQnKpOJSIuYMBMXlIrEcJGqgqTGEGCxpkVWlBxIYlJJZWkSogzPRExSGhd8iC62vY13bx2ENl9VQYO1qgbXhZySmHIms17dEEVbVHsXgocyb3y1Un7dQ_nGc96EfOM5__bc8-e76q5Ygfmlf8T-bbuu-a9r-zP-BfS5hEQ</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>Jiao, P. R.</creator><creator>Wei, L. M.</creator><creator>Cheng, Y. Q.</creator><creator>Yuan, R. Y.</creator><creator>Han, F.</creator><creator>Liang, J.</creator><creator>Liu, W. L.</creator><creator>Ren, T.</creator><creator>Xin, C. A.</creator><creator>Liao, M.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201210</creationdate><title>Molecular cloning, characterization, and expression analysis of the Muscovy duck Toll-like receptor 3 (MdTLR3) gene</title><author>Jiao, P. R. ; Wei, L. M. ; Cheng, Y. Q. ; Yuan, R. Y. ; Han, F. ; Liang, J. ; Liu, W. L. ; Ren, T. ; Xin, C. 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M.</creatorcontrib><creatorcontrib>Cheng, Y. Q.</creatorcontrib><creatorcontrib>Yuan, R. Y.</creatorcontrib><creatorcontrib>Han, F.</creatorcontrib><creatorcontrib>Liang, J.</creatorcontrib><creatorcontrib>Liu, W. L.</creatorcontrib><creatorcontrib>Ren, T.</creatorcontrib><creatorcontrib>Xin, C. A.</creatorcontrib><creatorcontrib>Liao, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Poultry science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiao, P. R.</au><au>Wei, L. M.</au><au>Cheng, Y. Q.</au><au>Yuan, R. Y.</au><au>Han, F.</au><au>Liang, J.</au><au>Liu, W. L.</au><au>Ren, T.</au><au>Xin, C. A.</au><au>Liao, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning, characterization, and expression analysis of the Muscovy duck Toll-like receptor 3 (MdTLR3) gene</atitle><jtitle>Poultry science</jtitle><addtitle>Poult Sci</addtitle><date>2012-10</date><risdate>2012</risdate><volume>91</volume><issue>10</issue><spage>2475</spage><epage>2481</epage><pages>2475-2481</pages><issn>0032-5791</issn><eissn>1525-3171</eissn><abstract>ABSTRACT
Toll-like receptor 3 (TLR3) is an important membrane-bound receptor for recognizing double-stranded RNA in innate immunity. In this study, we described the cloning and characterization of the Muscovy duck TLR3 (MdTLR3) gene. The full-length MdTLR3 cDNA (2,836 bp) encoded a polypeptide of 895 amino acids. The deduced amino acid sequence contained 4 main structural domains: a signal peptide, an extracellular leucine rich repeats domain, a transmembrane domain, and a Toll/IL-1 receptor domain. Quantitative real-time PCR analysis indicated that MdTLR3 mRNA was constitutively expressed in all sampled tissues of uninfected Muscovy duck except muscle. Expression of MdTLR3 in brain was significantly upregulated at 24 h (1.94-fold, P < 0.05), reached a peak at 48 h (4.64-fold, P < 0.05), and recovered to normal levels at 72 h postinfection with the H5N1 highly pathogenic avian influenza virus. In contrast, MdTLR3 expression was downregulated during the test period in spleen and lung. These results implicated MdTLR3 was a novel member of the TLR family, which is involved in the early stage of antiviral innate immunity.</abstract><cop>Oxford, UK</cop><pub>Oxford University Press</pub><pmid>22991530</pmid><doi>10.3382/ps.2012-02394</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals Cloning, Molecular Ducks - genetics Ducks - metabolism Gene Expression Regulation - physiology Influenza A Virus, H5N1 Subtype Influenza in Birds - metabolism Influenza in Birds - virology Molecular Sequence Data RNA, Messenger - genetics RNA, Messenger - metabolism Toll-Like Receptor 3 - genetics Toll-Like Receptor 3 - metabolism |
| title | Molecular cloning, characterization, and expression analysis of the Muscovy duck Toll-like receptor 3 (MdTLR3) gene |
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