Loop-mediated isothermal amplification (LAMP) method based on two species-specific primer sets for the rapid identification of Chinese Babesia bovis and B. bigemina

Abstract Bovine babesiosis is a tick-transmitted hemoprotozoan disease that is mainly caused by Babesia bovis and/or Babesia bigemina and is characterized by significant morbidity and mortality worldwide. This disease is widespread in most parts of China. However, it is difficult to rapidly discrimi...

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Veröffentlicht in:	Parasitology international 2012-12, Vol.61 (4), p.658-663
Hauptverfasser:	Liu, Aihong, Guan, Guiquan, Du, Pengfei, Gou, Huitian, Liu, Zhijie, Liu, Junlong, Ma, Milin, Yang, Jifei, Li, Youquan, Niu, Qinli, Ren, Qiaoyun, Bai, Qi, Yin, Hong, Luo, Jianxun
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaplasma marginale Animals Anopheles sinensis Babesia - classification Babesia - genetics Babesia bigemina Babesia bovis babesiosis Babesiosis - epidemiology Babesiosis - parasitology Babesiosis - veterinary Cattle China - epidemiology cross reaction Detection Differentiation DNA DNA primers DNA, Intergenic - genetics Gastroenterology and Hepatology genes Infectious Disease internal transcribed spacers LAMP leukocytes loop-mediated isothermal amplification morbidity mortality Nucleic Acid Amplification Techniques - methods parasites parasitology polymerase chain reaction Polymerase Chain Reaction - methods Sensitivity and Specificity Species Specificity Theileria annulata Theileria sergenti
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