Preferential emergence of cell types expressing markers for primitive endoderm lineages in mouse embryonic stem cells expressing exogenous EGAM1 homeoprotein
Embryonic stem (ES) cells have been considered as a valuable renewable source of materials in regenerative medicine. Recently, we identified the homeoprotein EGAM1 both in preimplantation mouse embryos and mouse ES cells. Expression of the Egam1 transcript and its encoded protein was detectable in d...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2012-09, Vol.114 (3), p.342-346 |
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| creator | Soma, Miki Iha, Momoe Kihara, Yumi Sato, Shiori Sato, Yuki Sato, Sho Mori, Yuki Sugawara, Saiko Kasuga, Kano Kojima, Ikuo Kobayashi, Masayuki |
| description | Embryonic stem (ES) cells have been considered as a valuable renewable source of materials in regenerative medicine. Recently, we identified the homeoprotein EGAM1 both in preimplantation mouse embryos and mouse ES cells. Expression of the Egam1 transcript and its encoded protein was detectable in differentiating mouse ES cells, while it was almost undetectable in undifferentiated cells. In the present study, in order to clarify the effect of forced expression of EGAM1 on the differentiation of mouse ES cells in vitro, transfectants expressing exogenous EGAM1 were generated. Egam1 transfectants promoted differentiation into cell types expressing Gata6, Gata4, Afp, or Plat, genes associated with emergence of the extra-embryonic endoderm lineages. On the other hand, Egam1 transfectants inhibited the expression of specific genes for the embryonic lineages, including Fgf5 (epiblast) and T (mesoderm), in addition to Cdx2, a specific gene for the extra-embryonic trophectoderm lineages. Changes in the percentage of cells recognizing by antibodies against specific marker proteins closely correlated with the expression patterns of their transcripts. Taken together, the results obtained in this study suggested that mouse ES cells expressing exogenous EGAM1 preferentially differentiate into extra-embryonic primitive endoderm lineages, rather than embryonic lineages or extra-embryonic trophectoderm lineages. |
| doi_str_mv | 10.1016/j.jbiosc.2012.04.012 |
| format | Article |
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Recently, we identified the homeoprotein EGAM1 both in preimplantation mouse embryos and mouse ES cells. Expression of the Egam1 transcript and its encoded protein was detectable in differentiating mouse ES cells, while it was almost undetectable in undifferentiated cells. In the present study, in order to clarify the effect of forced expression of EGAM1 on the differentiation of mouse ES cells in vitro, transfectants expressing exogenous EGAM1 were generated. Egam1 transfectants promoted differentiation into cell types expressing Gata6, Gata4, Afp, or Plat, genes associated with emergence of the extra-embryonic endoderm lineages. On the other hand, Egam1 transfectants inhibited the expression of specific genes for the embryonic lineages, including Fgf5 (epiblast) and T (mesoderm), in addition to Cdx2, a specific gene for the extra-embryonic trophectoderm lineages. Changes in the percentage of cells recognizing by antibodies against specific marker proteins closely correlated with the expression patterns of their transcripts. Taken together, the results obtained in this study suggested that mouse ES cells expressing exogenous EGAM1 preferentially differentiate into extra-embryonic primitive endoderm lineages, rather than embryonic lineages or extra-embryonic trophectoderm lineages.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2012.04.012</identifier><identifier>PMID: 22608553</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies ; Biological and medical sciences ; Biomarkers - metabolism ; Biotechnology ; cell differentiation ; Cell Differentiation - genetics ; Cell Line ; Cell Lineage ; Cell Shape ; Crxos1 ; Differentiation ; Egam1 ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Endoderm - cytology ; Endoderm - metabolism ; Fundamental and applied biological sciences. Psychology ; GATA transcription factors ; Gene Expression ; genes ; homeodomain proteins ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Mesoderm - metabolism ; Mice ; Primitive endoderm ; Transfection</subject><ispartof>Journal of bioscience and bioengineering, 2012-09, Vol.114 (3), p.342-346</ispartof><rights>2012 The Society for Biotechnology, Japan</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-3e1468713d81f1b431e304e3fca457677b8f63804546f18f5f981d021568a89d3</citedby><cites>FETCH-LOGICAL-c539t-3e1468713d81f1b431e304e3fca457677b8f63804546f18f5f981d021568a89d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1389172312001612$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26394764$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22608553$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soma, Miki</creatorcontrib><creatorcontrib>Iha, Momoe</creatorcontrib><creatorcontrib>Kihara, Yumi</creatorcontrib><creatorcontrib>Sato, Shiori</creatorcontrib><creatorcontrib>Sato, Yuki</creatorcontrib><creatorcontrib>Sato, Sho</creatorcontrib><creatorcontrib>Mori, Yuki</creatorcontrib><creatorcontrib>Sugawara, Saiko</creatorcontrib><creatorcontrib>Kasuga, Kano</creatorcontrib><creatorcontrib>Kojima, Ikuo</creatorcontrib><creatorcontrib>Kobayashi, Masayuki</creatorcontrib><title>Preferential emergence of cell types expressing markers for primitive endoderm lineages in mouse embryonic stem cells expressing exogenous EGAM1 homeoprotein</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>Embryonic stem (ES) cells have been considered as a valuable renewable source of materials in regenerative medicine. Recently, we identified the homeoprotein EGAM1 both in preimplantation mouse embryos and mouse ES cells. Expression of the Egam1 transcript and its encoded protein was detectable in differentiating mouse ES cells, while it was almost undetectable in undifferentiated cells. In the present study, in order to clarify the effect of forced expression of EGAM1 on the differentiation of mouse ES cells in vitro, transfectants expressing exogenous EGAM1 were generated. Egam1 transfectants promoted differentiation into cell types expressing Gata6, Gata4, Afp, or Plat, genes associated with emergence of the extra-embryonic endoderm lineages. On the other hand, Egam1 transfectants inhibited the expression of specific genes for the embryonic lineages, including Fgf5 (epiblast) and T (mesoderm), in addition to Cdx2, a specific gene for the extra-embryonic trophectoderm lineages. Changes in the percentage of cells recognizing by antibodies against specific marker proteins closely correlated with the expression patterns of their transcripts. Taken together, the results obtained in this study suggested that mouse ES cells expressing exogenous EGAM1 preferentially differentiate into extra-embryonic primitive endoderm lineages, rather than embryonic lineages or extra-embryonic trophectoderm lineages.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Biotechnology</subject><subject>cell differentiation</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Line</subject><subject>Cell Lineage</subject><subject>Cell Shape</subject><subject>Crxos1</subject><subject>Differentiation</subject><subject>Egam1</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Endoderm - cytology</subject><subject>Endoderm - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GATA transcription factors</subject><subject>Gene Expression</subject><subject>genes</subject><subject>homeodomain proteins</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Mesoderm - metabolism</subject><subject>Mice</subject><subject>Primitive endoderm</subject><subject>Transfection</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhiMEoqXwBgh8QeKS4Ikdx7kgVVUpSEUgQc-W44wXL4m92Nmq-zC8Kw5ZQFwQp7Hkb-b_Nf8UxVOgFVAQr7bVtnchmaqmUFeUV7ncK06B8bbkvIb7y1t2JbQ1OykepbSlFFrawsPipK4FlU3DTovvHyNajOhnp0eCE8YNeoMkWGJwHMl82GEieLeLmJLzGzLp-BVjIjZEsotucrO7RYJ-CAPGiYzOo97kFufJFPYpf019PATvDEkzTj-n_jUQ70KWzCi5vDp_D-RLmDDsYpjR-cfFA6vHhE-O9ay4eXP5-eJtef3h6t3F-XVpGtbNJUPgQrbABgkWes4AGeXIrNG8aUXb9tIKJilvuLAgbWM7CQOtoRFSy25gZ8XLdW7W_bbHNKvJpcWp9pidKaBMChBUyP9B65Y10PCM8hU1MaSU96yWhel4yJBaMlRbtWaolgwV5SqX3PbsqLDvJxx-N_0KLQMvjoBORo82am9c-sMJ1vFWLPrPV87qoPQmZubmU1YSNF8CY3KRer0SmJd76zCqZNyS_-AimlkNwf3b6w-DVscz</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Soma, Miki</creator><creator>Iha, Momoe</creator><creator>Kihara, Yumi</creator><creator>Sato, Shiori</creator><creator>Sato, Yuki</creator><creator>Sato, Sho</creator><creator>Mori, Yuki</creator><creator>Sugawara, Saiko</creator><creator>Kasuga, Kano</creator><creator>Kojima, Ikuo</creator><creator>Kobayashi, Masayuki</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120901</creationdate><title>Preferential emergence of cell types expressing markers for primitive endoderm lineages in mouse embryonic stem cells expressing exogenous EGAM1 homeoprotein</title><author>Soma, Miki ; Iha, Momoe ; Kihara, Yumi ; Sato, Shiori ; Sato, Yuki ; Sato, Sho ; Mori, Yuki ; Sugawara, Saiko ; Kasuga, Kano ; Kojima, Ikuo ; Kobayashi, Masayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-3e1468713d81f1b431e304e3fca457677b8f63804546f18f5f981d021568a89d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Biotechnology</topic><topic>cell differentiation</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Line</topic><topic>Cell Lineage</topic><topic>Cell Shape</topic><topic>Crxos1</topic><topic>Differentiation</topic><topic>Egam1</topic><topic>Embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Endoderm - cytology</topic><topic>Endoderm - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GATA transcription factors</topic><topic>Gene Expression</topic><topic>genes</topic><topic>homeodomain proteins</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Mesoderm - metabolism</topic><topic>Mice</topic><topic>Primitive endoderm</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soma, Miki</creatorcontrib><creatorcontrib>Iha, Momoe</creatorcontrib><creatorcontrib>Kihara, Yumi</creatorcontrib><creatorcontrib>Sato, Shiori</creatorcontrib><creatorcontrib>Sato, Yuki</creatorcontrib><creatorcontrib>Sato, Sho</creatorcontrib><creatorcontrib>Mori, Yuki</creatorcontrib><creatorcontrib>Sugawara, Saiko</creatorcontrib><creatorcontrib>Kasuga, Kano</creatorcontrib><creatorcontrib>Kojima, Ikuo</creatorcontrib><creatorcontrib>Kobayashi, Masayuki</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soma, Miki</au><au>Iha, Momoe</au><au>Kihara, Yumi</au><au>Sato, Shiori</au><au>Sato, Yuki</au><au>Sato, Sho</au><au>Mori, Yuki</au><au>Sugawara, Saiko</au><au>Kasuga, Kano</au><au>Kojima, Ikuo</au><au>Kobayashi, Masayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preferential emergence of cell types expressing markers for primitive endoderm lineages in mouse embryonic stem cells expressing exogenous EGAM1 homeoprotein</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>114</volume><issue>3</issue><spage>342</spage><epage>346</epage><pages>342-346</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Embryonic stem (ES) cells have been considered as a valuable renewable source of materials in regenerative medicine. 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Changes in the percentage of cells recognizing by antibodies against specific marker proteins closely correlated with the expression patterns of their transcripts. Taken together, the results obtained in this study suggested that mouse ES cells expressing exogenous EGAM1 preferentially differentiate into extra-embryonic primitive endoderm lineages, rather than embryonic lineages or extra-embryonic trophectoderm lineages.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22608553</pmid><doi>10.1016/j.jbiosc.2012.04.012</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of bioscience and bioengineering, 2012-09, Vol.114 (3), p.342-346 |
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| language | eng |
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| subjects | Animals Antibodies Biological and medical sciences Biomarkers - metabolism Biotechnology cell differentiation Cell Differentiation - genetics Cell Line Cell Lineage Cell Shape Crxos1 Differentiation Egam1 Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Endoderm - cytology Endoderm - metabolism Fundamental and applied biological sciences. Psychology GATA transcription factors Gene Expression genes homeodomain proteins Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Mesoderm - metabolism Mice Primitive endoderm Transfection |
| title | Preferential emergence of cell types expressing markers for primitive endoderm lineages in mouse embryonic stem cells expressing exogenous EGAM1 homeoprotein |
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