Urease expression in a Thermoanaerobacterium saccharolyticum ethanologen allows high titer ethanol production

Genes encoding the enzyme urease were integrated in a Thermoanaerobacterium saccharolyticum ethanologen. The engineered strain hydrolyzed urea, as evidenced by increased cellular growth and elevated final pH in urea minimal medium and urease activity in cell free extracts. Interestingly, replacement...

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Veröffentlicht in:	Metabolic engineering 2012-09, Vol.14 (5), p.528-532
Hauptverfasser:	Joe Shaw, A., Covalla, Sean F., Miller, Bethany B., Firliet, Brian T., Hogsett, David A., Herring, Christopher D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Biofuels Clostridium thermocellum - enzymology Clostridium thermocellum - genetics Enzymes Ethanol Ethanol - metabolism Fermentation Gene Expression Hydrogen-Ion Concentration metabolic engineering Osmotic Pressure pH effects Salts Stress Thermoanaerobacterium - enzymology Thermoanaerobacterium - genetics Thermoanaerobacterium - growth & development Thermophiles Urea Urea - metabolism Urease Urease - biosynthesis Urease - genetics
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