Analysis of Immune Response in Yellowtail upon Vaccination with a Bivalent Vaccine of Vibrio anguillarum and Lactococcus garvieae
We inoculated yellowtail Seriola quinqueradiata with a commercial bivalent vaccine containing inactivated Vibrio anguillarum and Lactococcus garvieae to better understand the immunological responses to the vaccination. Serum samples were collected from vaccinated and control (PBS-injected) fish at 1...
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| Veröffentlicht in: | Fish Pathology 2012, Vol.47(1), pp.12-19 |
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| creator | Nakajima, Nao Kijima, Mayumi Kawanishi, Michiko Katou, Goushi Futami, Kunihiko Katagiri, Takayuki Endo, Makoto Maita, Masashi |
| description | We inoculated yellowtail Seriola quinqueradiata with a commercial bivalent vaccine containing inactivated Vibrio anguillarum and Lactococcus garvieae to better understand the immunological responses to the vaccination. Serum samples were collected from vaccinated and control (PBS-injected) fish at 1, 3, 5, 7, 10, and 14 days post-vaccination (dpv). Compared to the controls, the serum from the vaccinated fish inhibited the proliferation of V. anguillarum from 7 dpv. However, vaccination had no effect on L. garvieae proliferation. The antibody titer against V. anguillarum obviously increased from 7 dpv, but no such increase was detected for L. garvieae. We confirmed that the protection conferred by the V. anguillarum vaccine was a result of the antibodies against the pathogen. However, humoral factors related to protection were not observed for L. garvieae. The expression of immune-related genes in the head kidney was profiled by microarray analysis. Twenty-two immune-related genes were up-regulated during the experimental period. Up-regulation of cellular immune-related genes, such as tumor necrosis factor, CC chemokine, and macrophage colony-stimulating factor receptor, was mostly observed from 1 to 3 dpv, whereas that of humoral immune-related genes was observed from 3 to 14 dpv. Notably, immunoglobulin-related genes showed continuous up-regulation during the 2-week experimental period. |
| doi_str_mv | 10.3147/jsfp.47.12 |
| format | Article |
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Serum samples were collected from vaccinated and control (PBS-injected) fish at 1, 3, 5, 7, 10, and 14 days post-vaccination (dpv). Compared to the controls, the serum from the vaccinated fish inhibited the proliferation of V. anguillarum from 7 dpv. However, vaccination had no effect on L. garvieae proliferation. The antibody titer against V. anguillarum obviously increased from 7 dpv, but no such increase was detected for L. garvieae. We confirmed that the protection conferred by the V. anguillarum vaccine was a result of the antibodies against the pathogen. However, humoral factors related to protection were not observed for L. garvieae. The expression of immune-related genes in the head kidney was profiled by microarray analysis. Twenty-two immune-related genes were up-regulated during the experimental period. Up-regulation of cellular immune-related genes, such as tumor necrosis factor, CC chemokine, and macrophage colony-stimulating factor receptor, was mostly observed from 1 to 3 dpv, whereas that of humoral immune-related genes was observed from 3 to 14 dpv. Notably, immunoglobulin-related genes showed continuous up-regulation during the 2-week experimental period.</description><identifier>ISSN: 0388-788X</identifier><identifier>EISSN: 1881-7335</identifier><identifier>DOI: 10.3147/jsfp.47.12</identifier><language>eng</language><publisher>The Japanese Society of Fish Pathology</publisher><subject>Gene expression ; Immune response ; Lactococcus garvieae ; Marine ; Seriola quinqueradiata ; Vaccine ; Vibrio anguillarum ; Yellowtail</subject><ispartof>Fish Pathology, 2012, Vol.47(1), pp.12-19</ispartof><rights>2012 The Japanese Society of Fish Pathology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-431bddfd04f2d66fd2df4b8a1bead367dca8e488696b2ddd6cb0bbe59c51bc213</citedby><cites>FETCH-LOGICAL-c356t-431bddfd04f2d66fd2df4b8a1bead367dca8e488696b2ddd6cb0bbe59c51bc213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Nakajima, Nao</creatorcontrib><creatorcontrib>Kijima, Mayumi</creatorcontrib><creatorcontrib>Kawanishi, Michiko</creatorcontrib><creatorcontrib>Katou, Goushi</creatorcontrib><creatorcontrib>Futami, Kunihiko</creatorcontrib><creatorcontrib>Katagiri, Takayuki</creatorcontrib><creatorcontrib>Endo, Makoto</creatorcontrib><creatorcontrib>Maita, Masashi</creatorcontrib><title>Analysis of Immune Response in Yellowtail upon Vaccination with a Bivalent Vaccine of Vibrio anguillarum and Lactococcus garvieae</title><title>Fish Pathology</title><addtitle>Fish Pathol.</addtitle><description>We inoculated yellowtail Seriola quinqueradiata with a commercial bivalent vaccine containing inactivated Vibrio anguillarum and Lactococcus garvieae to better understand the immunological responses to the vaccination. Serum samples were collected from vaccinated and control (PBS-injected) fish at 1, 3, 5, 7, 10, and 14 days post-vaccination (dpv). Compared to the controls, the serum from the vaccinated fish inhibited the proliferation of V. anguillarum from 7 dpv. However, vaccination had no effect on L. garvieae proliferation. The antibody titer against V. anguillarum obviously increased from 7 dpv, but no such increase was detected for L. garvieae. We confirmed that the protection conferred by the V. anguillarum vaccine was a result of the antibodies against the pathogen. However, humoral factors related to protection were not observed for L. garvieae. The expression of immune-related genes in the head kidney was profiled by microarray analysis. Twenty-two immune-related genes were up-regulated during the experimental period. Up-regulation of cellular immune-related genes, such as tumor necrosis factor, CC chemokine, and macrophage colony-stimulating factor receptor, was mostly observed from 1 to 3 dpv, whereas that of humoral immune-related genes was observed from 3 to 14 dpv. Notably, immunoglobulin-related genes showed continuous up-regulation during the 2-week experimental period.</description><subject>Gene expression</subject><subject>Immune response</subject><subject>Lactococcus garvieae</subject><subject>Marine</subject><subject>Seriola quinqueradiata</subject><subject>Vaccine</subject><subject>Vibrio anguillarum</subject><subject>Yellowtail</subject><issn>0388-788X</issn><issn>1881-7335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpFkM1LAzEQxYMoWKoX_4IcRdi62exHehIVv6AgiIqelkkyWyPZpCbZikf_c7dU9DJvmPfjwTxCjlg-46xsTt9jt5qVzYwVO2TChGBZw3m1SyY5FyJrhHjZJ4cxGpnnRSnKqplPyPe5A_sVTaS-o3d9PzikDxhX3kWkxtFXtNZ_JjCWDuORPoNSxkEy4_5p0hsFemHWYNGlXw83Sc9GBuMpuOVgrIUw9OOu6QJU8sorNUS6hLA2CHhA9jqwEQ9_dUqerq8eL2-zxf3N3eX5IlO8qlNWcia17nRedoWu604XuiulACYRNK8brUBgKUQ9r2Whta6VzKXEaq4qJlXB-JQcb3NXwX8MGFPbm6jG98ChH2LLxpZqVvB8g55sURV8jAG7dhVMD-FrhNpN1e2m6nbUkZ-Ssy38HhMs8Q-FkIyy-I9uByv-HPUGoUXHfwB9CY0N</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Nakajima, Nao</creator><creator>Kijima, Mayumi</creator><creator>Kawanishi, Michiko</creator><creator>Katou, Goushi</creator><creator>Futami, Kunihiko</creator><creator>Katagiri, Takayuki</creator><creator>Endo, Makoto</creator><creator>Maita, Masashi</creator><general>The Japanese Society of Fish Pathology</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope></search><sort><creationdate>2012</creationdate><title>Analysis of Immune Response in Yellowtail upon Vaccination with a Bivalent Vaccine of Vibrio anguillarum and Lactococcus garvieae</title><author>Nakajima, Nao ; Kijima, Mayumi ; Kawanishi, Michiko ; Katou, Goushi ; Futami, Kunihiko ; Katagiri, Takayuki ; Endo, Makoto ; Maita, Masashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-431bddfd04f2d66fd2df4b8a1bead367dca8e488696b2ddd6cb0bbe59c51bc213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Gene expression</topic><topic>Immune response</topic><topic>Lactococcus garvieae</topic><topic>Marine</topic><topic>Seriola quinqueradiata</topic><topic>Vaccine</topic><topic>Vibrio anguillarum</topic><topic>Yellowtail</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakajima, Nao</creatorcontrib><creatorcontrib>Kijima, Mayumi</creatorcontrib><creatorcontrib>Kawanishi, Michiko</creatorcontrib><creatorcontrib>Katou, Goushi</creatorcontrib><creatorcontrib>Futami, Kunihiko</creatorcontrib><creatorcontrib>Katagiri, Takayuki</creatorcontrib><creatorcontrib>Endo, Makoto</creatorcontrib><creatorcontrib>Maita, Masashi</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Fish Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakajima, Nao</au><au>Kijima, Mayumi</au><au>Kawanishi, Michiko</au><au>Katou, Goushi</au><au>Futami, Kunihiko</au><au>Katagiri, Takayuki</au><au>Endo, Makoto</au><au>Maita, Masashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Immune Response in Yellowtail upon Vaccination with a Bivalent Vaccine of Vibrio anguillarum and Lactococcus garvieae</atitle><jtitle>Fish Pathology</jtitle><addtitle>Fish Pathol.</addtitle><date>2012</date><risdate>2012</risdate><volume>47</volume><issue>1</issue><spage>12</spage><epage>19</epage><pages>12-19</pages><issn>0388-788X</issn><eissn>1881-7335</eissn><abstract>We inoculated yellowtail Seriola quinqueradiata with a commercial bivalent vaccine containing inactivated Vibrio anguillarum and Lactococcus garvieae to better understand the immunological responses to the vaccination. Serum samples were collected from vaccinated and control (PBS-injected) fish at 1, 3, 5, 7, 10, and 14 days post-vaccination (dpv). Compared to the controls, the serum from the vaccinated fish inhibited the proliferation of V. anguillarum from 7 dpv. However, vaccination had no effect on L. garvieae proliferation. The antibody titer against V. anguillarum obviously increased from 7 dpv, but no such increase was detected for L. garvieae. We confirmed that the protection conferred by the V. anguillarum vaccine was a result of the antibodies against the pathogen. However, humoral factors related to protection were not observed for L. garvieae. The expression of immune-related genes in the head kidney was profiled by microarray analysis. Twenty-two immune-related genes were up-regulated during the experimental period. Up-regulation of cellular immune-related genes, such as tumor necrosis factor, CC chemokine, and macrophage colony-stimulating factor receptor, was mostly observed from 1 to 3 dpv, whereas that of humoral immune-related genes was observed from 3 to 14 dpv. Notably, immunoglobulin-related genes showed continuous up-regulation during the 2-week experimental period.</abstract><pub>The Japanese Society of Fish Pathology</pub><doi>10.3147/jsfp.47.12</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Gene expression Immune response Lactococcus garvieae Marine Seriola quinqueradiata Vaccine Vibrio anguillarum Yellowtail |
| title | Analysis of Immune Response in Yellowtail upon Vaccination with a Bivalent Vaccine of Vibrio anguillarum and Lactococcus garvieae |
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