Generation of recombinant bacillus Calmette–Guérin and Mycobacterium smegmatis expressing BfpA and intimin as vaccine vectors against enteropathogenic Escherichia coli

Highlights ► We constructed rSmeg_pMIP_( bfp A or intimin ) and rBCG_pMIP_( bfpA or intimin ) vectors. ► The vectors contain the bfp a and intimin encoding genes. ► The vectors are stable, replicate and express BfpA and intimin. ► Vectors induce effective immune response measured by specific antibod...

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Veröffentlicht in:	Vaccine 2012-09, Vol.30 (41), p.5999-6005
Hauptverfasser:	Vasconcellos, Halyka Luzorio Franzotti, Scaramuzzi, Karina, Nascimento, Ivan Pereira, Da Costa Ferreira, Jorge M, Abe, Cecilia M, Piazza, Roxane M.F, Kipnis, Andre, Dias da Silva, Wilmar
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesins, Bacterial - genetics Adhesins, Bacterial - immunology adhesion Allergy and Immunology Animals antibodies Antibodies, Bacterial - blood BCG BCG Vaccine - administration & dosage BCG Vaccine - immunology bfpA and intimin blood serum Cell Line children clones Diarrhea DNA enteropathogenic Escherichia coli Enteropathogenic Escherichia coli - immunology Enteropathogenic Escherichia coli - pathogenicity EPEC Epithelial Cells - cytology Epithelial Cells - microbiology Escherichia coli Escherichia coli Proteins - genetics Escherichia coli Proteins - immunology feces Female Fimbriae Proteins - genetics Fimbriae Proteins - immunology genes Genetic Vectors Humans immune response immunoblotting immunoglobulin A immunoglobulin G interferon-gamma Interferon-gamma - immunology intestinal mucosa intimin kanamycin M. smegmatis Mice Mice, Inbred C57BL Mycobacterium bovis Mycobacterium bovis - genetics Mycobacterium bovis - immunology Mycobacterium bovis BCG Mycobacterium smegmatis Mycobacterium smegmatis - genetics Mycobacterium smegmatis - immunology polymerase chain reaction Recombinant vaccine Spleen - cytology Spleen - immunology splenocytes tumor necrosis factor-alpha Tumor Necrosis Factor-alpha - immunology vaccines Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology virulence
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