Fucoidan extract derived from Undaria pinnatifida inhibits angiogenesis by human umbilical vein endothelial cells
In recent years, anti-angiogenic therapy has become an effective strategy for inhibiting tumor growth. Fucoidan is a class of fucose-enriched sulfated polysaccharides found in brown algae, and it is known to have strong anti-tumor property. Using a human umbilical vein endothelial cells (HUVEC)-base...
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| Veröffentlicht in: | Phytomedicine (Stuttgart) 2012-06, Vol.19 (8-9), p.797-803 |
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| creator | Liu, Fang Wang, Jia Chang, Alan K. Liu, Bing Yang, Lili Li, Qiaomei Wang, Peisheng Zou, Xiangyang |
| description | In recent years, anti-angiogenic therapy has become an effective strategy for inhibiting tumor growth. Fucoidan is a class of fucose-enriched sulfated polysaccharides found in brown algae, and it is known to have strong anti-tumor property. Using a human umbilical vein endothelial cells (HUVEC)-based cell culture model, the present study investigated the anti-angiogenic activity of fucoidan extracted from the brown seaweed Undaria pinnatifida. Treatment of HUVECs with various concentrations of fucoidan resulted in significant inhibition of cell proliferation, cell migration, tube formation and vascular network formation. However, significant inhibition of cell proliferation only occurred with longer treatment time (48h instead of 24h or less). About 40% of cell proliferation and cell migration and 61% of tube formation by HUVECs were inhibited by 400μg/ml fucoidan, the maximum concentration tested. These results appeared to suggest that modulation of angiogenesis by fucoidan might not occur through growth inhibition and apoptosis. Ex vivo angiogenesis assay demonstrated that at 100μg/ml, fucoidan caused significant reduction in microvessel outgrowth. Western blot and RT-PCR analyses indicated that at 400μg/ml, fucoidan significantly reduced the expression of the angiogenesis factor VEGF-A in the suppression of angiogenesis activity. Our results showed that fucoidan isolated from U. pinnatifida may have a new therapeutic potential in the prevention angiogenesis-related diseases. |
| doi_str_mv | 10.1016/j.phymed.2012.03.015 |
| format | Article |
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Fucoidan is a class of fucose-enriched sulfated polysaccharides found in brown algae, and it is known to have strong anti-tumor property. Using a human umbilical vein endothelial cells (HUVEC)-based cell culture model, the present study investigated the anti-angiogenic activity of fucoidan extracted from the brown seaweed Undaria pinnatifida. Treatment of HUVECs with various concentrations of fucoidan resulted in significant inhibition of cell proliferation, cell migration, tube formation and vascular network formation. However, significant inhibition of cell proliferation only occurred with longer treatment time (48h instead of 24h or less). About 40% of cell proliferation and cell migration and 61% of tube formation by HUVECs were inhibited by 400μg/ml fucoidan, the maximum concentration tested. These results appeared to suggest that modulation of angiogenesis by fucoidan might not occur through growth inhibition and apoptosis. Ex vivo angiogenesis assay demonstrated that at 100μg/ml, fucoidan caused significant reduction in microvessel outgrowth. Western blot and RT-PCR analyses indicated that at 400μg/ml, fucoidan significantly reduced the expression of the angiogenesis factor VEGF-A in the suppression of angiogenesis activity. Our results showed that fucoidan isolated from U. pinnatifida may have a new therapeutic potential in the prevention angiogenesis-related diseases.</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1016/j.phymed.2012.03.015</identifier><identifier>PMID: 22510492</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Algae ; Angiogenesis ; Angiogenesis Inhibitors - pharmacology ; Animals ; Aorta - drug effects ; Apoptosis ; Apoptosis - drug effects ; Cell culture ; Cell migration ; Cell Movement - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Chemical properties ; Drug Evaluation, Preclinical - methods ; Endothelial cells ; Female ; Fucoidan ; Health aspects ; Human umbilical vein endothelial cells ; Human Umbilical Vein Endothelial Cells - drug effects ; Humans ; In Vitro Techniques ; Materia medica, Vegetable ; Neovascularization ; Neovascularization, Physiologic - drug effects ; Physiological aspects ; Plant extracts ; Polymerase chain reaction ; Polysaccharides ; Polysaccharides - pharmacology ; Rats ; Rats, Sprague-Dawley ; Seaweeds ; Tumors ; Umbilical cord ; umbilical vein ; Undaria - chemistry ; Undaria pinnatifida ; Vascular Endothelial Growth Factor A - genetics ; VEGF-A ; Wakame ; Western blotting</subject><ispartof>Phytomedicine (Stuttgart), 2012-06, Vol.19 (8-9), p.797-803</ispartof><rights>2012 Elsevier GmbH</rights><rights>Copyright © 2012 Elsevier GmbH. All rights reserved.</rights><rights>COPYRIGHT 2012 Urban & Fischer Verlag</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c590t-6c3ded48a2010e3dbd94511b7d2fe056a0ab1d9c42453556dcb98fa91f0668d73</citedby><cites>FETCH-LOGICAL-c590t-6c3ded48a2010e3dbd94511b7d2fe056a0ab1d9c42453556dcb98fa91f0668d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.phymed.2012.03.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22510492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Fang</creatorcontrib><creatorcontrib>Wang, Jia</creatorcontrib><creatorcontrib>Chang, Alan K.</creatorcontrib><creatorcontrib>Liu, Bing</creatorcontrib><creatorcontrib>Yang, Lili</creatorcontrib><creatorcontrib>Li, Qiaomei</creatorcontrib><creatorcontrib>Wang, Peisheng</creatorcontrib><creatorcontrib>Zou, Xiangyang</creatorcontrib><title>Fucoidan extract derived from Undaria pinnatifida inhibits angiogenesis by human umbilical vein endothelial cells</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>In recent years, anti-angiogenic therapy has become an effective strategy for inhibiting tumor growth. Fucoidan is a class of fucose-enriched sulfated polysaccharides found in brown algae, and it is known to have strong anti-tumor property. Using a human umbilical vein endothelial cells (HUVEC)-based cell culture model, the present study investigated the anti-angiogenic activity of fucoidan extracted from the brown seaweed Undaria pinnatifida. Treatment of HUVECs with various concentrations of fucoidan resulted in significant inhibition of cell proliferation, cell migration, tube formation and vascular network formation. However, significant inhibition of cell proliferation only occurred with longer treatment time (48h instead of 24h or less). About 40% of cell proliferation and cell migration and 61% of tube formation by HUVECs were inhibited by 400μg/ml fucoidan, the maximum concentration tested. These results appeared to suggest that modulation of angiogenesis by fucoidan might not occur through growth inhibition and apoptosis. Ex vivo angiogenesis assay demonstrated that at 100μg/ml, fucoidan caused significant reduction in microvessel outgrowth. Western blot and RT-PCR analyses indicated that at 400μg/ml, fucoidan significantly reduced the expression of the angiogenesis factor VEGF-A in the suppression of angiogenesis activity. Our results showed that fucoidan isolated from U. pinnatifida may have a new therapeutic potential in the prevention angiogenesis-related diseases.</description><subject>Algae</subject><subject>Angiogenesis</subject><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Aorta - drug effects</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cell culture</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Chemical properties</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Endothelial cells</subject><subject>Female</subject><subject>Fucoidan</subject><subject>Health aspects</subject><subject>Human umbilical vein endothelial cells</subject><subject>Human Umbilical Vein Endothelial Cells - drug effects</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Materia medica, Vegetable</subject><subject>Neovascularization</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Physiological aspects</subject><subject>Plant extracts</subject><subject>Polymerase chain reaction</subject><subject>Polysaccharides</subject><subject>Polysaccharides - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Seaweeds</subject><subject>Tumors</subject><subject>Umbilical cord</subject><subject>umbilical vein</subject><subject>Undaria - chemistry</subject><subject>Undaria pinnatifida</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>VEGF-A</subject><subject>Wakame</subject><subject>Western blotting</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksGL1DAUxoMo7rr6H4gWvHhpTZombS_CsrgqLHjQAW8hTV5n3tAms0k77Pz3pnT3IAwiOQRefu_jey8fIW8ZLRhl8tO-OOxOI9iipKwsKC8oE8_IJZOsyWkrfj8nl7StqrxmjF-QVzHuKWVVW9OX5KIsBaNVW16S-9vZeLTaZfAwBW2mzELAI9isD37MNs7qgDo7oHN6wj6RGboddjjFTLst-i04iBiz7pTt5jHpzGOHAxo9ZEfAJOusn3YwYCoYGIb4mrzo9RDhzeN9RTa3X37dfMvvfnz9fnN9lxvR0imXhluwVaPTeBS47WxbCca62pY9UCE11R2zranKSnAhpDVd2_S6ZT2VsrE1vyIfV91D8PczxEmNGBcH2oGfo2KUN5IxwZv_QFnTSM4rmdAPK7rVAyh0vV-2tuDqmiesLmvZJio_Qy2rCnrwDnpM5b_44gyfjoURzdmGam0wwccYoFeHgKMOp-R1sSvVXq3xUEs8FOUqxSO1vXscdO6Wt6empzwk4P0K9NorvQ0Y1eZnUpApO7UUYpH4vBKQ_u6IEFQ0CM6AxQBmUtbjvz38AVs-1ho</recordid><startdate>20120615</startdate><enddate>20120615</enddate><creator>Liu, Fang</creator><creator>Wang, Jia</creator><creator>Chang, Alan K.</creator><creator>Liu, Bing</creator><creator>Yang, Lili</creator><creator>Li, Qiaomei</creator><creator>Wang, Peisheng</creator><creator>Zou, Xiangyang</creator><general>Elsevier GmbH</general><general>Urban & Fischer Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>20120615</creationdate><title>Fucoidan extract derived from Undaria pinnatifida inhibits angiogenesis by human umbilical vein endothelial cells</title><author>Liu, Fang ; Wang, Jia ; Chang, Alan K. ; Liu, Bing ; Yang, Lili ; Li, Qiaomei ; Wang, Peisheng ; Zou, Xiangyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c590t-6c3ded48a2010e3dbd94511b7d2fe056a0ab1d9c42453556dcb98fa91f0668d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Algae</topic><topic>Angiogenesis</topic><topic>Angiogenesis Inhibitors - pharmacology</topic><topic>Animals</topic><topic>Aorta - drug effects</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Cell culture</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Chemical properties</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>Endothelial cells</topic><topic>Female</topic><topic>Fucoidan</topic><topic>Health aspects</topic><topic>Human umbilical vein endothelial cells</topic><topic>Human Umbilical Vein Endothelial Cells - drug effects</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Materia medica, Vegetable</topic><topic>Neovascularization</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Physiological aspects</topic><topic>Plant extracts</topic><topic>Polymerase chain reaction</topic><topic>Polysaccharides</topic><topic>Polysaccharides - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Seaweeds</topic><topic>Tumors</topic><topic>Umbilical cord</topic><topic>umbilical vein</topic><topic>Undaria - chemistry</topic><topic>Undaria pinnatifida</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>VEGF-A</topic><topic>Wakame</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Fang</creatorcontrib><creatorcontrib>Wang, Jia</creatorcontrib><creatorcontrib>Chang, Alan K.</creatorcontrib><creatorcontrib>Liu, Bing</creatorcontrib><creatorcontrib>Yang, Lili</creatorcontrib><creatorcontrib>Li, Qiaomei</creatorcontrib><creatorcontrib>Wang, Peisheng</creatorcontrib><creatorcontrib>Zou, Xiangyang</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Fang</au><au>Wang, Jia</au><au>Chang, Alan K.</au><au>Liu, Bing</au><au>Yang, Lili</au><au>Li, Qiaomei</au><au>Wang, Peisheng</au><au>Zou, Xiangyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fucoidan extract derived from Undaria pinnatifida inhibits angiogenesis by human umbilical vein endothelial cells</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2012-06-15</date><risdate>2012</risdate><volume>19</volume><issue>8-9</issue><spage>797</spage><epage>803</epage><pages>797-803</pages><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>In recent years, anti-angiogenic therapy has become an effective strategy for inhibiting tumor growth. Fucoidan is a class of fucose-enriched sulfated polysaccharides found in brown algae, and it is known to have strong anti-tumor property. Using a human umbilical vein endothelial cells (HUVEC)-based cell culture model, the present study investigated the anti-angiogenic activity of fucoidan extracted from the brown seaweed Undaria pinnatifida. Treatment of HUVECs with various concentrations of fucoidan resulted in significant inhibition of cell proliferation, cell migration, tube formation and vascular network formation. However, significant inhibition of cell proliferation only occurred with longer treatment time (48h instead of 24h or less). About 40% of cell proliferation and cell migration and 61% of tube formation by HUVECs were inhibited by 400μg/ml fucoidan, the maximum concentration tested. These results appeared to suggest that modulation of angiogenesis by fucoidan might not occur through growth inhibition and apoptosis. Ex vivo angiogenesis assay demonstrated that at 100μg/ml, fucoidan caused significant reduction in microvessel outgrowth. Western blot and RT-PCR analyses indicated that at 400μg/ml, fucoidan significantly reduced the expression of the angiogenesis factor VEGF-A in the suppression of angiogenesis activity. Our results showed that fucoidan isolated from U. pinnatifida may have a new therapeutic potential in the prevention angiogenesis-related diseases.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>22510492</pmid><doi>10.1016/j.phymed.2012.03.015</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0944-7113 |
| ispartof | Phytomedicine (Stuttgart), 2012-06, Vol.19 (8-9), p.797-803 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Algae Angiogenesis Angiogenesis Inhibitors - pharmacology Animals Aorta - drug effects Apoptosis Apoptosis - drug effects Cell culture Cell migration Cell Movement - drug effects Cell proliferation Cell Proliferation - drug effects Chemical properties Drug Evaluation, Preclinical - methods Endothelial cells Female Fucoidan Health aspects Human umbilical vein endothelial cells Human Umbilical Vein Endothelial Cells - drug effects Humans In Vitro Techniques Materia medica, Vegetable Neovascularization Neovascularization, Physiologic - drug effects Physiological aspects Plant extracts Polymerase chain reaction Polysaccharides Polysaccharides - pharmacology Rats Rats, Sprague-Dawley Seaweeds Tumors Umbilical cord umbilical vein Undaria - chemistry Undaria pinnatifida Vascular Endothelial Growth Factor A - genetics VEGF-A Wakame Western blotting |
| title | Fucoidan extract derived from Undaria pinnatifida inhibits angiogenesis by human umbilical vein endothelial cells |
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