Green tea extract: Possible mechanism and antibacterial activity on skin pathogens

► Aqueous extract of green tea was studied against skin infection causing bacteria. ► Bacterial strains were sensitive to aqueous extract via disc diffusion assay. ► MIC was determined via NBT assay (0.156–0.313mg/ml). ► Aqueous extract was not toxic to Vero cell-line (via MTT assay). ► Aqueous extr...

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Veröffentlicht in:Food chemistry 2012-11, Vol.135 (2), p.672-675
Hauptverfasser: Sharma, Anjali, Gupta, Sonal, Sarethy, Indira P., Dang, Shweta, Gabrani, Reema
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container_end_page 675
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container_start_page 672
container_title Food chemistry
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creator Sharma, Anjali
Gupta, Sonal
Sarethy, Indira P.
Dang, Shweta
Gabrani, Reema
description ► Aqueous extract of green tea was studied against skin infection causing bacteria. ► Bacterial strains were sensitive to aqueous extract via disc diffusion assay. ► MIC was determined via NBT assay (0.156–0.313mg/ml). ► Aqueous extract was not toxic to Vero cell-line (via MTT assay). ► Aqueous extract inhibits the adhesion of bacteria to Vero cells at its MIC value. Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ⩾7mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156–0.313mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500μg/ml. The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value.
doi_str_mv 10.1016/j.foodchem.2012.04.143
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Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ⩾7mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156–0.313mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500μg/ml. 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The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value.</description><subject>Adhesion</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-microbial</subject><subject>Antibacterial activity</subject><subject>Aqueous extract</subject><subject>Bacillus subtilis</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - isolation &amp; purification</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Biological and medical sciences</subject><subject>Camellia sinensis</subject><subject>Camellia sinensis - chemistry</subject><subject>Catechin - analysis</subject><subject>Catechin - pharmacology</subject><subject>Cercopithecus aethiops</subject><subject>Coffee, tea and other stimulative beverage industries</subject><subject>Cytotoxicity</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Green tea</subject><subject>Humans</subject><subject>Microbial Sensitivity Tests</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - pharmacology</subject><subject>Skin Diseases, Bacterial - drug therapy</subject><subject>Skin Diseases, Bacterial - microbiology</subject><subject>Tea - chemistry</subject><subject>Vero cell line</subject><subject>Vero Cells</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQQIMo7rj6F5ZcBC_dpjrd6bQnZdFVWFBEzyFdqTgZ-2NMMov7780ws3r0UFRRvKqkHmNXIGoQoF7var-uDrc0142AphZtDa18xDage1n1om8es42QQlcaWnXBnqW0E0IUVj9lF02jVem3G_b1JhItPJPl9DtHi_kN_7KmFMaJ-Ey4tUtIM7eLK5HDWACKwU68FOEu5Hu-Ljz9DAvf27xdf9CSnrMn3k6JXpzzJfv-4f2364_V7eebT9fvbitshz5XugNwXvfjCGgb7VAR2MEOBNh7KcdmwNHZvhNKEg7oFHpouxZ940F46uQle3Xau4_rrwOlbOaQkKbJLrQekgEhtQIAeUTVCcVYbovkzT6G2cb7ApmjT7MzDz7N0acRrSk-y-DV-Y3DOJP7O_YgsAAvz4BNaCcf7YIh_eMUDKrrhsK9PXFUjNwFiiZhoAXJhUiYjVvD__7yB_AymEM</recordid><startdate>20121115</startdate><enddate>20121115</enddate><creator>Sharma, Anjali</creator><creator>Gupta, Sonal</creator><creator>Sarethy, Indira P.</creator><creator>Dang, Shweta</creator><creator>Gabrani, Reema</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20121115</creationdate><title>Green tea extract: Possible mechanism and antibacterial activity on skin pathogens</title><author>Sharma, Anjali ; Gupta, Sonal ; Sarethy, Indira P. ; Dang, Shweta ; Gabrani, Reema</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-8511df87bb1ca28dc6e1a9a9e1c7f33b29cbda75063ec9cd6cf1454cf2f10fe53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adhesion</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-microbial</topic><topic>Antibacterial activity</topic><topic>Aqueous extract</topic><topic>Bacillus subtilis</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - isolation &amp; purification</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Biological and medical sciences</topic><topic>Camellia sinensis</topic><topic>Camellia sinensis - chemistry</topic><topic>Catechin - analysis</topic><topic>Catechin - pharmacology</topic><topic>Cercopithecus aethiops</topic><topic>Coffee, tea and other stimulative beverage industries</topic><topic>Cytotoxicity</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. 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Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ⩾7mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156–0.313mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500μg/ml. 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subjects Adhesion
Animals
Anti-Bacterial Agents - pharmacology
Anti-microbial
Antibacterial activity
Aqueous extract
Bacillus subtilis
Bacteria - drug effects
Bacteria - isolation & purification
Bacterial Adhesion - drug effects
Biological and medical sciences
Camellia sinensis
Camellia sinensis - chemistry
Catechin - analysis
Catechin - pharmacology
Cercopithecus aethiops
Coffee, tea and other stimulative beverage industries
Cytotoxicity
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Green tea
Humans
Microbial Sensitivity Tests
Plant Extracts - analysis
Plant Extracts - pharmacology
Skin Diseases, Bacterial - drug therapy
Skin Diseases, Bacterial - microbiology
Tea - chemistry
Vero cell line
Vero Cells
title Green tea extract: Possible mechanism and antibacterial activity on skin pathogens
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