Green tea extract: Possible mechanism and antibacterial activity on skin pathogens
► Aqueous extract of green tea was studied against skin infection causing bacteria. ► Bacterial strains were sensitive to aqueous extract via disc diffusion assay. ► MIC was determined via NBT assay (0.156–0.313mg/ml). ► Aqueous extract was not toxic to Vero cell-line (via MTT assay). ► Aqueous extr...
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Veröffentlicht in: | Food chemistry 2012-11, Vol.135 (2), p.672-675 |
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description | ► Aqueous extract of green tea was studied against skin infection causing bacteria. ► Bacterial strains were sensitive to aqueous extract via disc diffusion assay. ► MIC was determined via NBT assay (0.156–0.313mg/ml). ► Aqueous extract was not toxic to Vero cell-line (via MTT assay). ► Aqueous extract inhibits the adhesion of bacteria to Vero cells at its MIC value.
Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ⩾7mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156–0.313mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500μg/ml. The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value. |
doi_str_mv | 10.1016/j.foodchem.2012.04.143 |
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Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ⩾7mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156–0.313mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500μg/ml. The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2012.04.143</identifier><identifier>PMID: 22868144</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adhesion ; Animals ; Anti-Bacterial Agents - pharmacology ; Anti-microbial ; Antibacterial activity ; Aqueous extract ; Bacillus subtilis ; Bacteria - drug effects ; Bacteria - isolation & purification ; Bacterial Adhesion - drug effects ; Biological and medical sciences ; Camellia sinensis ; Camellia sinensis - chemistry ; Catechin - analysis ; Catechin - pharmacology ; Cercopithecus aethiops ; Coffee, tea and other stimulative beverage industries ; Cytotoxicity ; Food industries ; Food microbiology ; Fundamental and applied biological sciences. Psychology ; Green tea ; Humans ; Microbial Sensitivity Tests ; Plant Extracts - analysis ; Plant Extracts - pharmacology ; Skin Diseases, Bacterial - drug therapy ; Skin Diseases, Bacterial - microbiology ; Tea - chemistry ; Vero cell line ; Vero Cells</subject><ispartof>Food chemistry, 2012-11, Vol.135 (2), p.672-675</ispartof><rights>2012 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-8511df87bb1ca28dc6e1a9a9e1c7f33b29cbda75063ec9cd6cf1454cf2f10fe53</citedby><cites>FETCH-LOGICAL-c497t-8511df87bb1ca28dc6e1a9a9e1c7f33b29cbda75063ec9cd6cf1454cf2f10fe53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodchem.2012.04.143$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26196559$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22868144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharma, Anjali</creatorcontrib><creatorcontrib>Gupta, Sonal</creatorcontrib><creatorcontrib>Sarethy, Indira P.</creatorcontrib><creatorcontrib>Dang, Shweta</creatorcontrib><creatorcontrib>Gabrani, Reema</creatorcontrib><title>Green tea extract: Possible mechanism and antibacterial activity on skin pathogens</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>► Aqueous extract of green tea was studied against skin infection causing bacteria. ► Bacterial strains were sensitive to aqueous extract via disc diffusion assay. ► MIC was determined via NBT assay (0.156–0.313mg/ml). ► Aqueous extract was not toxic to Vero cell-line (via MTT assay). ► Aqueous extract inhibits the adhesion of bacteria to Vero cells at its MIC value.
Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ⩾7mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156–0.313mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500μg/ml. The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value.</description><subject>Adhesion</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-microbial</subject><subject>Antibacterial activity</subject><subject>Aqueous extract</subject><subject>Bacillus subtilis</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Biological and medical sciences</subject><subject>Camellia sinensis</subject><subject>Camellia sinensis - chemistry</subject><subject>Catechin - analysis</subject><subject>Catechin - pharmacology</subject><subject>Cercopithecus aethiops</subject><subject>Coffee, tea and other stimulative beverage industries</subject><subject>Cytotoxicity</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Green tea</subject><subject>Humans</subject><subject>Microbial Sensitivity Tests</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - pharmacology</subject><subject>Skin Diseases, Bacterial - drug therapy</subject><subject>Skin Diseases, Bacterial - microbiology</subject><subject>Tea - chemistry</subject><subject>Vero cell line</subject><subject>Vero Cells</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQQIMo7rj6F5ZcBC_dpjrd6bQnZdFVWFBEzyFdqTgZ-2NMMov7780ws3r0UFRRvKqkHmNXIGoQoF7var-uDrc0142AphZtDa18xDage1n1om8es42QQlcaWnXBnqW0E0IUVj9lF02jVem3G_b1JhItPJPl9DtHi_kN_7KmFMaJ-Ey4tUtIM7eLK5HDWACKwU68FOEu5Hu-Ljz9DAvf27xdf9CSnrMn3k6JXpzzJfv-4f2364_V7eebT9fvbitshz5XugNwXvfjCGgb7VAR2MEOBNh7KcdmwNHZvhNKEg7oFHpouxZ940F46uQle3Xau4_rrwOlbOaQkKbJLrQekgEhtQIAeUTVCcVYbovkzT6G2cb7ApmjT7MzDz7N0acRrSk-y-DV-Y3DOJP7O_YgsAAvz4BNaCcf7YIh_eMUDKrrhsK9PXFUjNwFiiZhoAXJhUiYjVvD__7yB_AymEM</recordid><startdate>20121115</startdate><enddate>20121115</enddate><creator>Sharma, Anjali</creator><creator>Gupta, Sonal</creator><creator>Sarethy, Indira P.</creator><creator>Dang, Shweta</creator><creator>Gabrani, Reema</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20121115</creationdate><title>Green tea extract: Possible mechanism and antibacterial activity on skin pathogens</title><author>Sharma, Anjali ; Gupta, Sonal ; Sarethy, Indira P. ; Dang, Shweta ; Gabrani, Reema</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-8511df87bb1ca28dc6e1a9a9e1c7f33b29cbda75063ec9cd6cf1454cf2f10fe53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adhesion</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-microbial</topic><topic>Antibacterial activity</topic><topic>Aqueous extract</topic><topic>Bacillus subtilis</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - isolation & purification</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Biological and medical sciences</topic><topic>Camellia sinensis</topic><topic>Camellia sinensis - chemistry</topic><topic>Catechin - analysis</topic><topic>Catechin - pharmacology</topic><topic>Cercopithecus aethiops</topic><topic>Coffee, tea and other stimulative beverage industries</topic><topic>Cytotoxicity</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Green tea</topic><topic>Humans</topic><topic>Microbial Sensitivity Tests</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - pharmacology</topic><topic>Skin Diseases, Bacterial - drug therapy</topic><topic>Skin Diseases, Bacterial - microbiology</topic><topic>Tea - chemistry</topic><topic>Vero cell line</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Anjali</creatorcontrib><creatorcontrib>Gupta, Sonal</creatorcontrib><creatorcontrib>Sarethy, Indira P.</creatorcontrib><creatorcontrib>Dang, Shweta</creatorcontrib><creatorcontrib>Gabrani, Reema</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Anjali</au><au>Gupta, Sonal</au><au>Sarethy, Indira P.</au><au>Dang, Shweta</au><au>Gabrani, Reema</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green tea extract: Possible mechanism and antibacterial activity on skin pathogens</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2012-11-15</date><risdate>2012</risdate><volume>135</volume><issue>2</issue><spage>672</spage><epage>675</epage><pages>672-675</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>► Aqueous extract of green tea was studied against skin infection causing bacteria. ► Bacterial strains were sensitive to aqueous extract via disc diffusion assay. ► MIC was determined via NBT assay (0.156–0.313mg/ml). ► Aqueous extract was not toxic to Vero cell-line (via MTT assay). ► Aqueous extract inhibits the adhesion of bacteria to Vero cells at its MIC value.
Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ⩾7mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156–0.313mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500μg/ml. The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>22868144</pmid><doi>10.1016/j.foodchem.2012.04.143</doi><tpages>4</tpages></addata></record> |
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subjects | Adhesion Animals Anti-Bacterial Agents - pharmacology Anti-microbial Antibacterial activity Aqueous extract Bacillus subtilis Bacteria - drug effects Bacteria - isolation & purification Bacterial Adhesion - drug effects Biological and medical sciences Camellia sinensis Camellia sinensis - chemistry Catechin - analysis Catechin - pharmacology Cercopithecus aethiops Coffee, tea and other stimulative beverage industries Cytotoxicity Food industries Food microbiology Fundamental and applied biological sciences. Psychology Green tea Humans Microbial Sensitivity Tests Plant Extracts - analysis Plant Extracts - pharmacology Skin Diseases, Bacterial - drug therapy Skin Diseases, Bacterial - microbiology Tea - chemistry Vero cell line Vero Cells |
title | Green tea extract: Possible mechanism and antibacterial activity on skin pathogens |
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