Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7
[Display omitted] ► WISH was used to investigate the tissue localisation of Schistosoma mansoni VAL genes. ► SmVAL6 demonstrated a discrete expression pattern in oral and ventral suckers. ► SmVAL7 revealed a distinct staining pattern in the oesophageal gland. ► The diversity of SmVAL6 transcripts is...
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| Veröffentlicht in: | International journal for parasitology 2012-06, Vol.42 (7), p.613-620 |
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| container_title | International journal for parasitology |
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| creator | Rofatto, Henrique K. Parker-Manuel, Sophia J. Barbosa, Tereza C. Tararam, Cibele A. Alan Wilson, R. Leite, Luciana C.C. Farias, Leonardo P. |
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► WISH was used to investigate the tissue localisation of Schistosoma mansoni VAL genes. ► SmVAL6 demonstrated a discrete expression pattern in oral and ventral suckers. ► SmVAL7 revealed a distinct staining pattern in the oesophageal gland. ► The diversity of SmVAL6 transcripts is translated to the protein level.
The Schistosoma mansoni Venom Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-Coating Protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in host–pathogen interactions. Whole mount in situ hybridisation demonstrated a distinct expression pattern in oral and ventral suckers of adult worms for SmVAL6 and in the oesophageal gland for SmVAL7 transcripts, respectively. Additionally, immunocytochemistry analysis corroborated SmVAL7 expression in the oesophageal gland. Analysis of protein expression across the parasite’s life cycle revealed that the SmVAL6 protein is upregulated in cercariae and adult male worms. Furthermore, SmVAL6 protein was identified by mass spectrometry in tegument fractions of adult worms. Finally, we speculate on possible functions of these two SmVALs at the host–parasite interface. |
| doi_str_mv | 10.1016/j.ijpara.2012.04.008 |
| format | Article |
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► WISH was used to investigate the tissue localisation of Schistosoma mansoni VAL genes. ► SmVAL6 demonstrated a discrete expression pattern in oral and ventral suckers. ► SmVAL7 revealed a distinct staining pattern in the oesophageal gland. ► The diversity of SmVAL6 transcripts is translated to the protein level.
The Schistosoma mansoni Venom Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-Coating Protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in host–pathogen interactions. Whole mount in situ hybridisation demonstrated a distinct expression pattern in oral and ventral suckers of adult worms for SmVAL6 and in the oesophageal gland for SmVAL7 transcripts, respectively. Additionally, immunocytochemistry analysis corroborated SmVAL7 expression in the oesophageal gland. Analysis of protein expression across the parasite’s life cycle revealed that the SmVAL6 protein is upregulated in cercariae and adult male worms. Furthermore, SmVAL6 protein was identified by mass spectrometry in tegument fractions of adult worms. Finally, we speculate on possible functions of these two SmVALs at the host–parasite interface.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/j.ijpara.2012.04.008</identifier><identifier>PMID: 22575701</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Allergens - biosynthesis ; Animal Structures - chemistry ; Animals ; Antigens, Helminth - biosynthesis ; Biological and medical sciences ; DNA, Helminth - chemistry ; DNA, Helminth - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Expression Profiling ; Helminth Proteins - biosynthesis ; Immunocytochemistry (ICC) ; In Situ Hybridization ; Life cycle. Host-agent relationship. Pathogenesis ; Male ; Mass Spectrometry ; Molecular Sequence Data ; Protozoa ; Schistosoma mansoni ; Schistosoma mansoni - chemistry ; Schistosoma mansoni - genetics ; Schistosoma mansoni - growth & development ; Sequence Analysis, DNA ; SmVAL ; Whole-mount in situ hybridisation (WISH)</subject><ispartof>International journal for parasitology, 2012-06, Vol.42 (7), p.613-620</ispartof><rights>2012 Australian Society for Parasitology Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Australian Society for Parasitology Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-c3f5b6777f567fe1b62c7d2151d80f0087dc06b294ba15e42b08ef881a76e82d3</citedby><cites>FETCH-LOGICAL-c425t-c3f5b6777f567fe1b62c7d2151d80f0087dc06b294ba15e42b08ef881a76e82d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0020751912000896$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26079331$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22575701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rofatto, Henrique K.</creatorcontrib><creatorcontrib>Parker-Manuel, Sophia J.</creatorcontrib><creatorcontrib>Barbosa, Tereza C.</creatorcontrib><creatorcontrib>Tararam, Cibele A.</creatorcontrib><creatorcontrib>Alan Wilson, R.</creatorcontrib><creatorcontrib>Leite, Luciana C.C.</creatorcontrib><creatorcontrib>Farias, Leonardo P.</creatorcontrib><title>Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>[Display omitted]
► WISH was used to investigate the tissue localisation of Schistosoma mansoni VAL genes. ► SmVAL6 demonstrated a discrete expression pattern in oral and ventral suckers. ► SmVAL7 revealed a distinct staining pattern in the oesophageal gland. ► The diversity of SmVAL6 transcripts is translated to the protein level.
The Schistosoma mansoni Venom Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-Coating Protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in host–pathogen interactions. Whole mount in situ hybridisation demonstrated a distinct expression pattern in oral and ventral suckers of adult worms for SmVAL6 and in the oesophageal gland for SmVAL7 transcripts, respectively. Additionally, immunocytochemistry analysis corroborated SmVAL7 expression in the oesophageal gland. Analysis of protein expression across the parasite’s life cycle revealed that the SmVAL6 protein is upregulated in cercariae and adult male worms. Furthermore, SmVAL6 protein was identified by mass spectrometry in tegument fractions of adult worms. Finally, we speculate on possible functions of these two SmVALs at the host–parasite interface.</description><subject>Allergens - biosynthesis</subject><subject>Animal Structures - chemistry</subject><subject>Animals</subject><subject>Antigens, Helminth - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>DNA, Helminth - chemistry</subject><subject>DNA, Helminth - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Helminth Proteins - biosynthesis</subject><subject>Immunocytochemistry (ICC)</subject><subject>In Situ Hybridization</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Molecular Sequence Data</subject><subject>Protozoa</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - chemistry</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosoma mansoni - growth & development</subject><subject>Sequence Analysis, DNA</subject><subject>SmVAL</subject><subject>Whole-mount in situ hybridisation (WISH)</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFu1DAQhi0EotvCGyDkCxKXhLETx94LUlUBRVqJA6VXy3HG4CWxgyeL4O3Jahe4IU5z-f6Zfz7GngmoBYju1b6O-9kVV0sQsoa2BjAP2EYYva1ANOoh2wBIqLQS2wt2SbQHEKpp28fsQkqllQaxYfd3keiAHH_MBYliTnx2y4IlEc-Bf_RfIi2Z8uT45BLlFPk9pjzx63HE8hlTtYtfkc8lLxjXTMddGrh-wh4FNxI-Pc8r9untm7ub22r34d37m-td5Vuplso3QfWd1jqoTgcUfSe9HqRQYjAQ1of04KHr5bbtnVDYyh4MBmOE0x0aOTRX7OVp71rg2wFpsVMkj-PoEuYDWQGN6cA0oP8DlULIY5kVbU-oL5moYLBziZMrP1fIHuXbvT3Jt0f5Flq7dl1jz88XDv2Ew5_Qb9sr8OIMOPJuDMUlH-kv14HeNs2Re33icFX3PWKx5CMmj0Ms6Bc75PjvJr8AK_yjMg</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Rofatto, Henrique K.</creator><creator>Parker-Manuel, Sophia J.</creator><creator>Barbosa, Tereza C.</creator><creator>Tararam, Cibele A.</creator><creator>Alan Wilson, R.</creator><creator>Leite, Luciana C.C.</creator><creator>Farias, Leonardo P.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20120601</creationdate><title>Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7</title><author>Rofatto, Henrique K. ; Parker-Manuel, Sophia J. ; Barbosa, Tereza C. ; Tararam, Cibele A. ; Alan Wilson, R. ; Leite, Luciana C.C. ; Farias, Leonardo P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-c3f5b6777f567fe1b62c7d2151d80f0087dc06b294ba15e42b08ef881a76e82d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Allergens - biosynthesis</topic><topic>Animal Structures - chemistry</topic><topic>Animals</topic><topic>Antigens, Helminth - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>DNA, Helminth - chemistry</topic><topic>DNA, Helminth - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Helminth Proteins - biosynthesis</topic><topic>Immunocytochemistry (ICC)</topic><topic>In Situ Hybridization</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Molecular Sequence Data</topic><topic>Protozoa</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - chemistry</topic><topic>Schistosoma mansoni - genetics</topic><topic>Schistosoma mansoni - growth & development</topic><topic>Sequence Analysis, DNA</topic><topic>SmVAL</topic><topic>Whole-mount in situ hybridisation (WISH)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rofatto, Henrique K.</creatorcontrib><creatorcontrib>Parker-Manuel, Sophia J.</creatorcontrib><creatorcontrib>Barbosa, Tereza C.</creatorcontrib><creatorcontrib>Tararam, Cibele A.</creatorcontrib><creatorcontrib>Alan Wilson, R.</creatorcontrib><creatorcontrib>Leite, Luciana C.C.</creatorcontrib><creatorcontrib>Farias, Leonardo P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rofatto, Henrique K.</au><au>Parker-Manuel, Sophia J.</au><au>Barbosa, Tereza C.</au><au>Tararam, Cibele A.</au><au>Alan Wilson, R.</au><au>Leite, Luciana C.C.</au><au>Farias, Leonardo P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>42</volume><issue>7</issue><spage>613</spage><epage>620</epage><pages>613-620</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>[Display omitted]
► WISH was used to investigate the tissue localisation of Schistosoma mansoni VAL genes. ► SmVAL6 demonstrated a discrete expression pattern in oral and ventral suckers. ► SmVAL7 revealed a distinct staining pattern in the oesophageal gland. ► The diversity of SmVAL6 transcripts is translated to the protein level.
The Schistosoma mansoni Venom Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-Coating Protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in host–pathogen interactions. Whole mount in situ hybridisation demonstrated a distinct expression pattern in oral and ventral suckers of adult worms for SmVAL6 and in the oesophageal gland for SmVAL7 transcripts, respectively. Additionally, immunocytochemistry analysis corroborated SmVAL7 expression in the oesophageal gland. Analysis of protein expression across the parasite’s life cycle revealed that the SmVAL6 protein is upregulated in cercariae and adult male worms. Furthermore, SmVAL6 protein was identified by mass spectrometry in tegument fractions of adult worms. Finally, we speculate on possible functions of these two SmVALs at the host–parasite interface.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>22575701</pmid><doi>10.1016/j.ijpara.2012.04.008</doi><tpages>8</tpages></addata></record> |
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| subjects | Allergens - biosynthesis Animal Structures - chemistry Animals Antigens, Helminth - biosynthesis Biological and medical sciences DNA, Helminth - chemistry DNA, Helminth - genetics Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Profiling Helminth Proteins - biosynthesis Immunocytochemistry (ICC) In Situ Hybridization Life cycle. Host-agent relationship. Pathogenesis Male Mass Spectrometry Molecular Sequence Data Protozoa Schistosoma mansoni Schistosoma mansoni - chemistry Schistosoma mansoni - genetics Schistosoma mansoni - growth & development Sequence Analysis, DNA SmVAL Whole-mount in situ hybridisation (WISH) |
| title | Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7 |
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