Rapid identification of chloroplast haplotypes using High Resolution Melting analysis

We have evaluated High Resolution Melting (HRM) analysis as a method for one‐step haplotype identification in phylogeographic analysis. Using two adjoined internal amplicons (c. 360 and 390 bp) at the chloroplast rps16 intron (c. 750 bp) we applied HRM to identify haplotypes in 21 populations of two...

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Veröffentlicht in:	Molecular ecology resources 2012-09, Vol.12 (5), p.894-908
Hauptverfasser:	DANG, XIAO-DONG, KELLEHER, COLIN T., HOWARD-WILLIAMS, EMMA, MEADE, CONOR V.
Format:	Artikel
Sprache:	eng
Schlagworte:	angiosperms Arenaria Arenaria ciliata Caryophyllaceae Caryophyllaceae - classification Caryophyllaceae - genetics conservation genetics DNA, Chloroplast - chemistry DNA, Chloroplast - genetics DNA, Plant - chemistry DNA, Plant - genetics Haplotypes Molecular Biology - methods Molecular Sequence Data Nucleic Acid Denaturation Phylogeography Phylogeography - methods Polymorphism Polymorphism, Single Nucleotide population genetics-empirical Sensitivity and Specificity Sequence Analysis, DNA speciation Transition Temperature
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