Changes in hydrogen production and polymer accumulation upon sulfur-deprivation in purple photosynthetic bacteria

The work investigated physiological conditions directing cellular metabolism toward either H 2-production or storage polymer accumulation in purple photosynthetic bacteria. Hydrogen-producing cultures of the purple anoxygenic photosynthetic bacterium Rhodospirillum rubrum were resuspended in media l...

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Veröffentlicht in:International journal of hydrogen energy 2009-08, Vol.34 (15), p.6157-6170
Hauptverfasser: Melnicki, Matthew R., Eroglu, Ela, Melis, Anastasios
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container_issue 15
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container_title International journal of hydrogen energy
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creator Melnicki, Matthew R.
Eroglu, Ela
Melis, Anastasios
description The work investigated physiological conditions directing cellular metabolism toward either H 2-production or storage polymer accumulation in purple photosynthetic bacteria. Hydrogen-producing cultures of the purple anoxygenic photosynthetic bacterium Rhodospirillum rubrum were resuspended in media lacking sulfur (S) nutrients. S-deprived cultures displayed lack of growth, cessation of bacteriochlorophyll and protein accumulation, and inhibition of H 2 evolution. Cell volume increased substantially and large amounts of polymer were found to accumulate extracellularly. Poly-β-hydroxybutyrate (PHB) content increased about 3.5-fold within 24 h of S-deprivation. Most cells remained viable after 100 h of S-deprivation and cultures were capable of resuming growth and H 2-production when supplemented with sulfate. Transcript levels, protein amount, and activity of the nitrogenase enzyme, which are responsible for H 2-production, decreased with a halftime of about 15 h upon S-deprivation. In addition, the nitrogenase NifH subunits were modified by ADP-ribosylation, indicating post-translational inactivation. Comparative aconitase activity measurements of control and S-deprived cells failed to indicate a general stress to Fe–S proteins, as aconitase, a Fe–S protein in the citric acid cycle sensitive to oxidative stress, maintained activity throughout the course of the S-deprivation. In contrast to nifH transcriptional down-regulation, expression of cysK (encoding cysteine synthase) was upregulated in response to S-deprivation. The described physiology is not specific to R. rubrum, as Rhodobacter sphaeroides and Rhodopseudomonas palustris exhibited a similar response to S-deprivation. It is suggested that manipulation of the supply of S-nutrients may serve as a tool for the alternative production of H 2 or PHB in purple photosynthetic bacteria, thus affording opportunities to design photobiological systems that serve in both energy conversion and storage processes.
doi_str_mv 10.1016/j.ijhydene.2009.05.115
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Hydrogen-producing cultures of the purple anoxygenic photosynthetic bacterium Rhodospirillum rubrum were resuspended in media lacking sulfur (S) nutrients. S-deprived cultures displayed lack of growth, cessation of bacteriochlorophyll and protein accumulation, and inhibition of H 2 evolution. Cell volume increased substantially and large amounts of polymer were found to accumulate extracellularly. Poly-β-hydroxybutyrate (PHB) content increased about 3.5-fold within 24 h of S-deprivation. Most cells remained viable after 100 h of S-deprivation and cultures were capable of resuming growth and H 2-production when supplemented with sulfate. Transcript levels, protein amount, and activity of the nitrogenase enzyme, which are responsible for H 2-production, decreased with a halftime of about 15 h upon S-deprivation. In addition, the nitrogenase NifH subunits were modified by ADP-ribosylation, indicating post-translational inactivation. Comparative aconitase activity measurements of control and S-deprived cells failed to indicate a general stress to Fe–S proteins, as aconitase, a Fe–S protein in the citric acid cycle sensitive to oxidative stress, maintained activity throughout the course of the S-deprivation. In contrast to nifH transcriptional down-regulation, expression of cysK (encoding cysteine synthase) was upregulated in response to S-deprivation. The described physiology is not specific to R. rubrum, as Rhodobacter sphaeroides and Rhodopseudomonas palustris exhibited a similar response to S-deprivation. 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Hydrogen-producing cultures of the purple anoxygenic photosynthetic bacterium Rhodospirillum rubrum were resuspended in media lacking sulfur (S) nutrients. S-deprived cultures displayed lack of growth, cessation of bacteriochlorophyll and protein accumulation, and inhibition of H 2 evolution. Cell volume increased substantially and large amounts of polymer were found to accumulate extracellularly. Poly-β-hydroxybutyrate (PHB) content increased about 3.5-fold within 24 h of S-deprivation. Most cells remained viable after 100 h of S-deprivation and cultures were capable of resuming growth and H 2-production when supplemented with sulfate. Transcript levels, protein amount, and activity of the nitrogenase enzyme, which are responsible for H 2-production, decreased with a halftime of about 15 h upon S-deprivation. In addition, the nitrogenase NifH subunits were modified by ADP-ribosylation, indicating post-translational inactivation. Comparative aconitase activity measurements of control and S-deprived cells failed to indicate a general stress to Fe–S proteins, as aconitase, a Fe–S protein in the citric acid cycle sensitive to oxidative stress, maintained activity throughout the course of the S-deprivation. In contrast to nifH transcriptional down-regulation, expression of cysK (encoding cysteine synthase) was upregulated in response to S-deprivation. The described physiology is not specific to R. rubrum, as Rhodobacter sphaeroides and Rhodopseudomonas palustris exhibited a similar response to S-deprivation. It is suggested that manipulation of the supply of S-nutrients may serve as a tool for the alternative production of H 2 or PHB in purple photosynthetic bacteria, thus affording opportunities to design photobiological systems that serve in both energy conversion and storage processes.</description><subject>ADP-ribosylation</subject><subject>Alternative fuels. 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Production and utilization</topic><topic>Applied sciences</topic><topic>Energy</topic><topic>Exact sciences and technology</topic><topic>Fuels</topic><topic>Hydrogen</topic><topic>Hydrogen production</topic><topic>Nitrogenase</topic><topic>Poly-β-hydroxybutyrate</topic><topic>Purple photosynthetic bacteria</topic><topic>Rhodobacter sphaeroides</topic><topic>Rhodopseudomonas palustris</topic><topic>Rhodospirillum rubrum</topic><topic>Sulfur-deprivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Melnicki, Matthew R.</creatorcontrib><creatorcontrib>Eroglu, Ela</creatorcontrib><creatorcontrib>Melis, Anastasios</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of hydrogen energy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Melnicki, Matthew R.</au><au>Eroglu, Ela</au><au>Melis, Anastasios</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in hydrogen production and polymer accumulation upon sulfur-deprivation in purple photosynthetic bacteria</atitle><jtitle>International journal of hydrogen energy</jtitle><date>2009-08-01</date><risdate>2009</risdate><volume>34</volume><issue>15</issue><spage>6157</spage><epage>6170</epage><pages>6157-6170</pages><issn>0360-3199</issn><eissn>1879-3487</eissn><coden>IJHEDX</coden><abstract>The work investigated physiological conditions directing cellular metabolism toward either H 2-production or storage polymer accumulation in purple photosynthetic bacteria. 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subjects ADP-ribosylation
Alternative fuels. Production and utilization
Applied sciences
Energy
Exact sciences and technology
Fuels
Hydrogen
Hydrogen production
Nitrogenase
Poly-β-hydroxybutyrate
Purple photosynthetic bacteria
Rhodobacter sphaeroides
Rhodopseudomonas palustris
Rhodospirillum rubrum
Sulfur-deprivation
title Changes in hydrogen production and polymer accumulation upon sulfur-deprivation in purple photosynthetic bacteria
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