Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals
Abstract Studies of enterohemorrhagic Escherichia coli (EHEC) infection mechanisms using mammals require large numbers of animals and are both costly and associated with ethical problems. Here, we evaluated the pathogenic mechanisms of EHEC in the silkworm model. Injection of a clinically isolated E...
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| Veröffentlicht in: | FEMS microbiology letters 2012-08, Vol.333 (1), p.59-68 |
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| creator | Miyashita, Atsushi Iyoda, Sunao Ishii, Kenichi Hamamoto, Hiroshi Sekimizu, Kazuhisa Kaito, Chikara |
| description | Abstract
Studies of enterohemorrhagic Escherichia coli (EHEC) infection mechanisms using mammals require large numbers of animals and are both costly and associated with ethical problems. Here, we evaluated the pathogenic mechanisms of EHEC in the silkworm model. Injection of a clinically isolated EHEC O157:H7 Sakai into either the silkworm hemolymph or intraperitoneal fluid of mice killed the host animals. EHEC O157:H7 Sakai deletion mutants of the rfbE gene, which encodes perosamine synthetase, a monosaccharide component synthetase of the O-antigen, or deletion mutants of the waaL gene, which encodes O-antigen ligase against the lipid A-core region of lipopolysaccharide (LPS), had attenuated killing ability in both silkworms and mice. Introduction of the rfbE gene or the waaL gene into the respective mutants restored the killing ability in silkworms. Growth of both mutants was inhibited by a major antimicrobial peptide in the silkworm hemolymph, moricin. The viability of both mutants was decreased in swine serum. The bactericidal effect of swine serum against both mutants was inactivated by heat treatment. These findings suggest that the LPS O-antigen of EHEC O157:H7 plays an important defensive role against antimicrobial factors in the host body fluid and is thus essential to the lethal effects of EHEC in animals. |
| doi_str_mv | 10.1111/j.1574-6968.2012.02599.x |
| format | Article |
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Studies of enterohemorrhagic Escherichia coli (EHEC) infection mechanisms using mammals require large numbers of animals and are both costly and associated with ethical problems. Here, we evaluated the pathogenic mechanisms of EHEC in the silkworm model. Injection of a clinically isolated EHEC O157:H7 Sakai into either the silkworm hemolymph or intraperitoneal fluid of mice killed the host animals. EHEC O157:H7 Sakai deletion mutants of the rfbE gene, which encodes perosamine synthetase, a monosaccharide component synthetase of the O-antigen, or deletion mutants of the waaL gene, which encodes O-antigen ligase against the lipid A-core region of lipopolysaccharide (LPS), had attenuated killing ability in both silkworms and mice. Introduction of the rfbE gene or the waaL gene into the respective mutants restored the killing ability in silkworms. Growth of both mutants was inhibited by a major antimicrobial peptide in the silkworm hemolymph, moricin. The viability of both mutants was decreased in swine serum. The bactericidal effect of swine serum against both mutants was inactivated by heat treatment. These findings suggest that the LPS O-antigen of EHEC O157:H7 plays an important defensive role against antimicrobial factors in the host body fluid and is thus essential to the lethal effects of EHEC in animals.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2012.02599.x</identifier><identifier>PMID: 22612664</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adhesins, Bacterial - genetics ; Adhesins, Bacterial - metabolism ; Animals ; Anti-Bacterial Agents - immunology ; Antigens ; Antigens, Bacterial - immunology ; Antiinfectives and antibacterials ; Antimicrobial Cationic Peptides - immunology ; Antimicrobial peptides ; Bacteria ; Bacteriology ; Biological and medical sciences ; Body fluids ; Bombyx - immunology ; Bombyx - microbiology ; Bombyx mori ; Carbohydrate Epimerases - genetics ; Carbohydrate Epimerases - metabolism ; Carbon-Oxygen Ligases - genetics ; Carbon-Oxygen Ligases - metabolism ; Deletion mutant ; Disease Models, Animal ; E coli ; enterohemorrhagic Escherichia coli ; Escherichia coli ; Escherichia coli Infections - immunology ; Escherichia coli Infections - microbiology ; Escherichia coli O157 - genetics ; Escherichia coli O157 - growth & development ; Escherichia coli O157 - pathogenicity ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene deletion ; Genes, Bacterial ; Heat treatment ; Hemolymph ; Hemolymph - immunology ; Hot Temperature ; Insect Proteins - immunology ; Insects ; Lethal Dose 50 ; Lethal effects ; Lipid A ; Lipids ; lipopolysaccharide O‐antigen ; Lipopolysaccharides ; Mammals ; Mice ; Microbial Viability ; Microbiology ; Miscellaneous ; Monosaccharides ; O Antigens - genetics ; O Antigens - toxicity ; RfbE gene ; Sequence Deletion ; silkworm model ; Silkworms ; Swine ; Swine - immunology ; Transaminases - genetics ; Transaminases - metabolism ; Viability ; virulence</subject><ispartof>FEMS microbiology letters, 2012-08, Vol.333 (1), p.59-68</ispartof><rights>Copyright © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2012</rights><rights>2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>Copyright © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6209-758031711617b30cb3ae983ae929e5f842f1e2076c24edd666f3c7a66784e1013</citedby><cites>FETCH-LOGICAL-c6209-758031711617b30cb3ae983ae929e5f842f1e2076c24edd666f3c7a66784e1013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2012.02599.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2012.02599.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26128753$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22612664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyashita, Atsushi</creatorcontrib><creatorcontrib>Iyoda, Sunao</creatorcontrib><creatorcontrib>Ishii, Kenichi</creatorcontrib><creatorcontrib>Hamamoto, Hiroshi</creatorcontrib><creatorcontrib>Sekimizu, Kazuhisa</creatorcontrib><creatorcontrib>Kaito, Chikara</creatorcontrib><title>Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Studies of enterohemorrhagic Escherichia coli (EHEC) infection mechanisms using mammals require large numbers of animals and are both costly and associated with ethical problems. Here, we evaluated the pathogenic mechanisms of EHEC in the silkworm model. Injection of a clinically isolated EHEC O157:H7 Sakai into either the silkworm hemolymph or intraperitoneal fluid of mice killed the host animals. EHEC O157:H7 Sakai deletion mutants of the rfbE gene, which encodes perosamine synthetase, a monosaccharide component synthetase of the O-antigen, or deletion mutants of the waaL gene, which encodes O-antigen ligase against the lipid A-core region of lipopolysaccharide (LPS), had attenuated killing ability in both silkworms and mice. Introduction of the rfbE gene or the waaL gene into the respective mutants restored the killing ability in silkworms. Growth of both mutants was inhibited by a major antimicrobial peptide in the silkworm hemolymph, moricin. The viability of both mutants was decreased in swine serum. The bactericidal effect of swine serum against both mutants was inactivated by heat treatment. These findings suggest that the LPS O-antigen of EHEC O157:H7 plays an important defensive role against antimicrobial factors in the host body fluid and is thus essential to the lethal effects of EHEC in animals.</description><subject>Adhesins, Bacterial - genetics</subject><subject>Adhesins, Bacterial - metabolism</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - immunology</subject><subject>Antigens</subject><subject>Antigens, Bacterial - immunology</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial Cationic Peptides - immunology</subject><subject>Antimicrobial peptides</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Body fluids</subject><subject>Bombyx - immunology</subject><subject>Bombyx - microbiology</subject><subject>Bombyx mori</subject><subject>Carbohydrate Epimerases - genetics</subject><subject>Carbohydrate Epimerases - metabolism</subject><subject>Carbon-Oxygen Ligases - genetics</subject><subject>Carbon-Oxygen Ligases - metabolism</subject><subject>Deletion mutant</subject><subject>Disease Models, Animal</subject><subject>E coli</subject><subject>enterohemorrhagic Escherichia coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - immunology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli O157 - genetics</subject><subject>Escherichia coli O157 - growth & development</subject><subject>Escherichia coli O157 - pathogenicity</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene deletion</subject><subject>Genes, Bacterial</subject><subject>Heat treatment</subject><subject>Hemolymph</subject><subject>Hemolymph - immunology</subject><subject>Hot Temperature</subject><subject>Insect Proteins - immunology</subject><subject>Insects</subject><subject>Lethal Dose 50</subject><subject>Lethal effects</subject><subject>Lipid A</subject><subject>Lipids</subject><subject>lipopolysaccharide O‐antigen</subject><subject>Lipopolysaccharides</subject><subject>Mammals</subject><subject>Mice</subject><subject>Microbial Viability</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Monosaccharides</subject><subject>O Antigens - genetics</subject><subject>O Antigens - toxicity</subject><subject>RfbE gene</subject><subject>Sequence Deletion</subject><subject>silkworm model</subject><subject>Silkworms</subject><subject>Swine</subject><subject>Swine - immunology</subject><subject>Transaminases - genetics</subject><subject>Transaminases - metabolism</subject><subject>Viability</subject><subject>virulence</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkV2L1DAUhoMo7rj6FyQggjet-WiTVPBCll13YWRu9Dpk0tNpxrbpJi3u4J83dcZdUITNRRLI855zwoMQpiSnab3f57SURSYqoXJGKMsJK6sqv3uCVvcPT9GKcKkySip5hl7EuCeEFIyI5-iMMUGZEMUK_Vy70Y--O0RjbWuCqwFvMjNMbgcD9g2GYYLgW-h9CK3ZOYsvo20hONs6g63vHN6knh-uJXYRB7idXYAaNz7g767r3LDDWz-12A0R7BSxGWrcm743XXyJnjXpgFen8xx9u7r8enGdrTefby4-rTMrGKkyWSrCqaRUULnlxG65gUotG6ugbFTBGgqMSGFZAXUthGi4lUYIqQqghPJz9O5Ydwz-doY46d5FC11nBvBz1JRwJQgtSvkIlBUV57KsEvrmL3Tv5zCkj2jGSSlVRRlLlDpSNvgYAzR6DK434ZBK6cWl3utFmV6U6cWl_u1S36Xo61ODedtDfR_8Iy8Bb0-AidZ0TTCDdfGBS5iSJU_cxyP3w3VwePQA-urLermlPD_m_Tz-J539O_4vWgrHjw</recordid><startdate>201208</startdate><enddate>201208</enddate><creator>Miyashita, Atsushi</creator><creator>Iyoda, Sunao</creator><creator>Ishii, Kenichi</creator><creator>Hamamoto, Hiroshi</creator><creator>Sekimizu, Kazuhisa</creator><creator>Kaito, Chikara</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201208</creationdate><title>Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals</title><author>Miyashita, Atsushi ; Iyoda, Sunao ; Ishii, Kenichi ; Hamamoto, Hiroshi ; Sekimizu, Kazuhisa ; Kaito, Chikara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6209-758031711617b30cb3ae983ae929e5f842f1e2076c24edd666f3c7a66784e1013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adhesins, Bacterial - genetics</topic><topic>Adhesins, Bacterial - metabolism</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - immunology</topic><topic>Antigens</topic><topic>Antigens, Bacterial - immunology</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial Cationic Peptides - immunology</topic><topic>Antimicrobial peptides</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Body fluids</topic><topic>Bombyx - immunology</topic><topic>Bombyx - microbiology</topic><topic>Bombyx mori</topic><topic>Carbohydrate Epimerases - genetics</topic><topic>Carbohydrate Epimerases - metabolism</topic><topic>Carbon-Oxygen Ligases - genetics</topic><topic>Carbon-Oxygen Ligases - metabolism</topic><topic>Deletion mutant</topic><topic>Disease Models, Animal</topic><topic>E coli</topic><topic>enterohemorrhagic Escherichia coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - immunology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli O157 - genetics</topic><topic>Escherichia coli O157 - growth & development</topic><topic>Escherichia coli O157 - pathogenicity</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene deletion</topic><topic>Genes, Bacterial</topic><topic>Heat treatment</topic><topic>Hemolymph</topic><topic>Hemolymph - immunology</topic><topic>Hot Temperature</topic><topic>Insect Proteins - immunology</topic><topic>Insects</topic><topic>Lethal Dose 50</topic><topic>Lethal effects</topic><topic>Lipid A</topic><topic>Lipids</topic><topic>lipopolysaccharide O‐antigen</topic><topic>Lipopolysaccharides</topic><topic>Mammals</topic><topic>Mice</topic><topic>Microbial Viability</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Monosaccharides</topic><topic>O Antigens - genetics</topic><topic>O Antigens - toxicity</topic><topic>RfbE gene</topic><topic>Sequence Deletion</topic><topic>silkworm model</topic><topic>Silkworms</topic><topic>Swine</topic><topic>Swine - immunology</topic><topic>Transaminases - genetics</topic><topic>Transaminases - metabolism</topic><topic>Viability</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyashita, Atsushi</creatorcontrib><creatorcontrib>Iyoda, Sunao</creatorcontrib><creatorcontrib>Ishii, Kenichi</creatorcontrib><creatorcontrib>Hamamoto, Hiroshi</creatorcontrib><creatorcontrib>Sekimizu, Kazuhisa</creatorcontrib><creatorcontrib>Kaito, Chikara</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyashita, Atsushi</au><au>Iyoda, Sunao</au><au>Ishii, Kenichi</au><au>Hamamoto, Hiroshi</au><au>Sekimizu, Kazuhisa</au><au>Kaito, Chikara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2012-08</date><risdate>2012</risdate><volume>333</volume><issue>1</issue><spage>59</spage><epage>68</epage><pages>59-68</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Studies of enterohemorrhagic Escherichia coli (EHEC) infection mechanisms using mammals require large numbers of animals and are both costly and associated with ethical problems. Here, we evaluated the pathogenic mechanisms of EHEC in the silkworm model. Injection of a clinically isolated EHEC O157:H7 Sakai into either the silkworm hemolymph or intraperitoneal fluid of mice killed the host animals. EHEC O157:H7 Sakai deletion mutants of the rfbE gene, which encodes perosamine synthetase, a monosaccharide component synthetase of the O-antigen, or deletion mutants of the waaL gene, which encodes O-antigen ligase against the lipid A-core region of lipopolysaccharide (LPS), had attenuated killing ability in both silkworms and mice. Introduction of the rfbE gene or the waaL gene into the respective mutants restored the killing ability in silkworms. Growth of both mutants was inhibited by a major antimicrobial peptide in the silkworm hemolymph, moricin. The viability of both mutants was decreased in swine serum. The bactericidal effect of swine serum against both mutants was inactivated by heat treatment. These findings suggest that the LPS O-antigen of EHEC O157:H7 plays an important defensive role against antimicrobial factors in the host body fluid and is thus essential to the lethal effects of EHEC in animals.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22612664</pmid><doi>10.1111/j.1574-6968.2012.02599.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2012-08, Vol.333 (1), p.59-68 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1038601457 |
| source | MEDLINE; Wiley Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adhesins, Bacterial - genetics Adhesins, Bacterial - metabolism Animals Anti-Bacterial Agents - immunology Antigens Antigens, Bacterial - immunology Antiinfectives and antibacterials Antimicrobial Cationic Peptides - immunology Antimicrobial peptides Bacteria Bacteriology Biological and medical sciences Body fluids Bombyx - immunology Bombyx - microbiology Bombyx mori Carbohydrate Epimerases - genetics Carbohydrate Epimerases - metabolism Carbon-Oxygen Ligases - genetics Carbon-Oxygen Ligases - metabolism Deletion mutant Disease Models, Animal E coli enterohemorrhagic Escherichia coli Escherichia coli Escherichia coli Infections - immunology Escherichia coli Infections - microbiology Escherichia coli O157 - genetics Escherichia coli O157 - growth & development Escherichia coli O157 - pathogenicity Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene deletion Genes, Bacterial Heat treatment Hemolymph Hemolymph - immunology Hot Temperature Insect Proteins - immunology Insects Lethal Dose 50 Lethal effects Lipid A Lipids lipopolysaccharide O‐antigen Lipopolysaccharides Mammals Mice Microbial Viability Microbiology Miscellaneous Monosaccharides O Antigens - genetics O Antigens - toxicity RfbE gene Sequence Deletion silkworm model Silkworms Swine Swine - immunology Transaminases - genetics Transaminases - metabolism Viability virulence |
| title | Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T08%3A05%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lipopolysaccharide%20O-antigen%20of%20enterohemorrhagic%20Escherichia%20coli%20O157:H7%20is%20required%20for%20killing%20both%20insects%20and%20mammals&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Miyashita,%20Atsushi&rft.date=2012-08&rft.volume=333&rft.issue=1&rft.spage=59&rft.epage=68&rft.pages=59-68&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.2012.02599.x&rft_dat=%3Cproquest_cross%3E1038601457%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2305789122&rft_id=info:pmid/22612664&rft_oup_id=10.1111/j.1574-6968.2012.02599.x&rfr_iscdi=true |