Development of a quantitative real-time PCR for the detection of Tenacibaculum maritimum and its application to field samples

The development and the application of a quantitative real‐time PCR for the detection of Tenacibaculum maritimum are described. A set of primers and probe was designed to amplify a 155‐bp fragment specific to the T. maritimum 16S rRNA gene. The test was shown to be very sensitive, able to detect as...

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Veröffentlicht in:	Journal of fish diseases 2012-08, Vol.35 (8), p.579-590
Hauptverfasser:	Fringuelli, E, Savage, P D, Gordon, A, Baxter, E J, Rodger, H D, Graham, D A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Load Environmental Monitoring FFPE tissues fish Fish Diseases - diagnosis Fish Diseases - microbiology Fish Diseases - pathology Flavobacteriaceae Infections - diagnosis Flavobacteriaceae Infections - microbiology Flavobacteriaceae Infections - pathology Flavobacteriaceae Infections - veterinary gill Gills - microbiology Gills - pathology jellyfish Muggiaea atlantica Phialella quadrata real-time PCR Real-Time Polymerase Chain Reaction Reproducibility of Results RNA, Ribosomal, 16S - genetics Salmo salar Scyphozoa - microbiology Tenacibaculum - genetics Tenacibaculum maritimum
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