Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography
A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of antimycin A components from antimycin fermentation broth. Six antimycin A components were successfully purified for the first time by HSCCC with a two-phase solvent syste...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 2010-09, Vol.1217 (36), p.5687-5692 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5692 |
|---|---|
| container_issue | 36 |
| container_start_page | 5687 |
| container_title | Journal of Chromatography A |
| container_volume | 1217 |
| creator | Wang, Jiaoyan Wen, Yaoming Chen, Xiuming Lin, Yuhan Zhou, Jingming Xie, Ying Wang, Huaiwang Jiang, Hong Zheng, Wei |
| description | A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of antimycin A components from antimycin fermentation broth. Six antimycin A components were successfully purified for the first time by HSCCC with a two-phase solvent system composed of
n-hexane–ethyl acetate–methanol–water (5:2:4:1, by volume). Total of 20
mg antimycin A
4(a or b), 25
mg antimycin A
3(a or b), 21
mg antimycin A
8(a or b), 34
mg antimycin A
2(a or b), 26
mg antimycin A
1(a or b) and 34
mg antimycin A
1(a or b) with the purities of 93.2, 98.6, 96.2, 94.1, 94.9 and 96.7%, respectively, determined by high-performance liquid chromatography (HPLC), were yielded from 200
mg crude sample only in one HSCCC run. |
| doi_str_mv | 10.1016/j.chroma.2010.07.003 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1038594141</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967310008885</els_id><sourcerecordid>1038594141</sourcerecordid><originalsourceid>FETCH-LOGICAL-c514t-5a387db820bd35d90f1558c8f6065c7b3ad382c68fc0d335e42604e33972ef723</originalsourceid><addsrcrecordid>eNp9kU9v1DAQxS0EotuFb4DAF6Resh3HSey9IFUV_6RKVIKeLccZb7zaxMF2KvbcL46rLNATJ4_Gv-c3fkPIGwYbBqy53G9MH_ygNyXkFogNAH9GVkwKXnAh5HOyAihZsW0EPyPnMe4BmABRviRnJTRScl6tyMNtwEkHndw90ngq_Ui9pdH9onpMbjgaN9Iravww-RHHFKnNxk_uLIYh9xdlG3zqaXukvdv1RZwQuyydx4ShMHMIGaTL5Mnvgp764yvywupDxNenc03uPn38cf2luPn2-ev11U1halalotZciq6VJbQdr7stWFbX0kjbQFMb0XLdcVmaRloDHec1VmUDFXK-FSVaUfI1uVjenYL_OWNManDR4OGgR_RzVAy4rLcVq1hGqwU1wccY0KopuEGHY4bUY_xqr5ZPqMf4FQiV48-ytyeHuR2w-yv6k3cG3p8AHY0-2KBH4-I_jrNaNHlha_Ju4az2Su9CZu6-ZycOTMpsLzPxYSEwJ3bvMKhoHI4GOxfQJNV59_9ZfwPpHrD5</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1038594141</pqid></control><display><type>article</type><title>Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Wang, Jiaoyan ; Wen, Yaoming ; Chen, Xiuming ; Lin, Yuhan ; Zhou, Jingming ; Xie, Ying ; Wang, Huaiwang ; Jiang, Hong ; Zheng, Wei</creator><creatorcontrib>Wang, Jiaoyan ; Wen, Yaoming ; Chen, Xiuming ; Lin, Yuhan ; Zhou, Jingming ; Xie, Ying ; Wang, Huaiwang ; Jiang, Hong ; Zheng, Wei</creatorcontrib><description>A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of antimycin A components from antimycin fermentation broth. Six antimycin A components were successfully purified for the first time by HSCCC with a two-phase solvent system composed of
n-hexane–ethyl acetate–methanol–water (5:2:4:1, by volume). Total of 20
mg antimycin A
4(a or b), 25
mg antimycin A
3(a or b), 21
mg antimycin A
8(a or b), 34
mg antimycin A
2(a or b), 26
mg antimycin A
1(a or b) and 34
mg antimycin A
1(a or b) with the purities of 93.2, 98.6, 96.2, 94.1, 94.9 and 96.7%, respectively, determined by high-performance liquid chromatography (HPLC), were yielded from 200
mg crude sample only in one HSCCC run.</description><identifier>ISSN: 0021-9673</identifier><identifier>EISSN: 1873-3778</identifier><identifier>DOI: 10.1016/j.chroma.2010.07.003</identifier><identifier>PMID: 20688334</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetates - chemistry ; Antibiotics (antibacterial agents, antifungal agents) ; Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents ; Antimycin A - chemistry ; Antimycin A - isolation & purification ; Antimycin A - metabolism ; Antimycin A components ; Applied microbiology ; Biological and medical sciences ; Bioreactors ; Biotechnology ; Countercurrent Distribution - methods ; Culture Media, Conditioned - chemistry ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Hexanes - chemistry ; HSCCC ; Methanol - chemistry ; Methods. Procedures. Technologies ; Microbiology ; Others ; Separation ; Streptomyces - metabolism ; Various methods and equipments ; Water - chemistry</subject><ispartof>Journal of Chromatography A, 2010-09, Vol.1217 (36), p.5687-5692</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-5a387db820bd35d90f1558c8f6065c7b3ad382c68fc0d335e42604e33972ef723</citedby><cites>FETCH-LOGICAL-c514t-5a387db820bd35d90f1558c8f6065c7b3ad382c68fc0d335e42604e33972ef723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021967310008885$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23157667$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20688334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jiaoyan</creatorcontrib><creatorcontrib>Wen, Yaoming</creatorcontrib><creatorcontrib>Chen, Xiuming</creatorcontrib><creatorcontrib>Lin, Yuhan</creatorcontrib><creatorcontrib>Zhou, Jingming</creatorcontrib><creatorcontrib>Xie, Ying</creatorcontrib><creatorcontrib>Wang, Huaiwang</creatorcontrib><creatorcontrib>Jiang, Hong</creatorcontrib><creatorcontrib>Zheng, Wei</creatorcontrib><title>Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of antimycin A components from antimycin fermentation broth. Six antimycin A components were successfully purified for the first time by HSCCC with a two-phase solvent system composed of
n-hexane–ethyl acetate–methanol–water (5:2:4:1, by volume). Total of 20
mg antimycin A
4(a or b), 25
mg antimycin A
3(a or b), 21
mg antimycin A
8(a or b), 34
mg antimycin A
2(a or b), 26
mg antimycin A
1(a or b) and 34
mg antimycin A
1(a or b) with the purities of 93.2, 98.6, 96.2, 94.1, 94.9 and 96.7%, respectively, determined by high-performance liquid chromatography (HPLC), were yielded from 200
mg crude sample only in one HSCCC run.</description><subject>Acetates - chemistry</subject><subject>Antibiotics (antibacterial agents, antifungal agents)</subject><subject>Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents</subject><subject>Antimycin A - chemistry</subject><subject>Antimycin A - isolation & purification</subject><subject>Antimycin A - metabolism</subject><subject>Antimycin A components</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Countercurrent Distribution - methods</subject><subject>Culture Media, Conditioned - chemistry</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hexanes - chemistry</subject><subject>HSCCC</subject><subject>Methanol - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Others</subject><subject>Separation</subject><subject>Streptomyces - metabolism</subject><subject>Various methods and equipments</subject><subject>Water - chemistry</subject><issn>0021-9673</issn><issn>1873-3778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EotuFb4DAF6Resh3HSey9IFUV_6RKVIKeLccZb7zaxMF2KvbcL46rLNATJ4_Gv-c3fkPIGwYbBqy53G9MH_ygNyXkFogNAH9GVkwKXnAh5HOyAihZsW0EPyPnMe4BmABRviRnJTRScl6tyMNtwEkHndw90ngq_Ui9pdH9onpMbjgaN9Iravww-RHHFKnNxk_uLIYh9xdlG3zqaXukvdv1RZwQuyydx4ShMHMIGaTL5Mnvgp764yvywupDxNenc03uPn38cf2luPn2-ev11U1halalotZciq6VJbQdr7stWFbX0kjbQFMb0XLdcVmaRloDHec1VmUDFXK-FSVaUfI1uVjenYL_OWNManDR4OGgR_RzVAy4rLcVq1hGqwU1wccY0KopuEGHY4bUY_xqr5ZPqMf4FQiV48-ytyeHuR2w-yv6k3cG3p8AHY0-2KBH4-I_jrNaNHlha_Ju4az2Su9CZu6-ZycOTMpsLzPxYSEwJ3bvMKhoHI4GOxfQJNV59_9ZfwPpHrD5</recordid><startdate>20100903</startdate><enddate>20100903</enddate><creator>Wang, Jiaoyan</creator><creator>Wen, Yaoming</creator><creator>Chen, Xiuming</creator><creator>Lin, Yuhan</creator><creator>Zhou, Jingming</creator><creator>Xie, Ying</creator><creator>Wang, Huaiwang</creator><creator>Jiang, Hong</creator><creator>Zheng, Wei</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QO</scope><scope>7T7</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20100903</creationdate><title>Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography</title><author>Wang, Jiaoyan ; Wen, Yaoming ; Chen, Xiuming ; Lin, Yuhan ; Zhou, Jingming ; Xie, Ying ; Wang, Huaiwang ; Jiang, Hong ; Zheng, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-5a387db820bd35d90f1558c8f6065c7b3ad382c68fc0d335e42604e33972ef723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetates - chemistry</topic><topic>Antibiotics (antibacterial agents, antifungal agents)</topic><topic>Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents</topic><topic>Antimycin A - chemistry</topic><topic>Antimycin A - isolation & purification</topic><topic>Antimycin A - metabolism</topic><topic>Antimycin A components</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Countercurrent Distribution - methods</topic><topic>Culture Media, Conditioned - chemistry</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hexanes - chemistry</topic><topic>HSCCC</topic><topic>Methanol - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Others</topic><topic>Separation</topic><topic>Streptomyces - metabolism</topic><topic>Various methods and equipments</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jiaoyan</creatorcontrib><creatorcontrib>Wen, Yaoming</creatorcontrib><creatorcontrib>Chen, Xiuming</creatorcontrib><creatorcontrib>Lin, Yuhan</creatorcontrib><creatorcontrib>Zhou, Jingming</creatorcontrib><creatorcontrib>Xie, Ying</creatorcontrib><creatorcontrib>Wang, Huaiwang</creatorcontrib><creatorcontrib>Jiang, Hong</creatorcontrib><creatorcontrib>Zheng, Wei</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jiaoyan</au><au>Wen, Yaoming</au><au>Chen, Xiuming</au><au>Lin, Yuhan</au><au>Zhou, Jingming</au><au>Xie, Ying</au><au>Wang, Huaiwang</au><au>Jiang, Hong</au><au>Zheng, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2010-09-03</date><risdate>2010</risdate><volume>1217</volume><issue>36</issue><spage>5687</spage><epage>5692</epage><pages>5687-5692</pages><issn>0021-9673</issn><eissn>1873-3778</eissn><coden>JOCRAM</coden><abstract>A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of antimycin A components from antimycin fermentation broth. Six antimycin A components were successfully purified for the first time by HSCCC with a two-phase solvent system composed of
n-hexane–ethyl acetate–methanol–water (5:2:4:1, by volume). Total of 20
mg antimycin A
4(a or b), 25
mg antimycin A
3(a or b), 21
mg antimycin A
8(a or b), 34
mg antimycin A
2(a or b), 26
mg antimycin A
1(a or b) and 34
mg antimycin A
1(a or b) with the purities of 93.2, 98.6, 96.2, 94.1, 94.9 and 96.7%, respectively, determined by high-performance liquid chromatography (HPLC), were yielded from 200
mg crude sample only in one HSCCC run.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20688334</pmid><doi>10.1016/j.chroma.2010.07.003</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 2010-09, Vol.1217 (36), p.5687-5692 |
| issn | 0021-9673 1873-3778 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1038594141 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetates - chemistry Antibiotics (antibacterial agents, antifungal agents) Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents Antimycin A - chemistry Antimycin A - isolation & purification Antimycin A - metabolism Antimycin A components Applied microbiology Biological and medical sciences Bioreactors Biotechnology Countercurrent Distribution - methods Culture Media, Conditioned - chemistry Fermentation Fundamental and applied biological sciences. Psychology Hexanes - chemistry HSCCC Methanol - chemistry Methods. Procedures. Technologies Microbiology Others Separation Streptomyces - metabolism Various methods and equipments Water - chemistry |
| title | Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T17%3A52%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparative%20separation%20of%20six%20antimycin%20A%20components%20from%20antimycin%20fermentation%20broth%20by%20high-speed%20counter-current%20chromatography&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=Wang,%20Jiaoyan&rft.date=2010-09-03&rft.volume=1217&rft.issue=36&rft.spage=5687&rft.epage=5692&rft.pages=5687-5692&rft.issn=0021-9673&rft.eissn=1873-3778&rft.coden=JOCRAM&rft_id=info:doi/10.1016/j.chroma.2010.07.003&rft_dat=%3Cproquest_cross%3E1038594141%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1038594141&rft_id=info:pmid/20688334&rft_els_id=S0021967310008885&rfr_iscdi=true |