Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway
[Display omitted] ► HDGF stimulates podosome formation, dorsal ruffles formation and cell migration. ► PI3K/Akt signaling cascade is involved in the HDGF-mediated cell migration. ► Ad-PTENT inhibits HDGF-mediated cell migration and PI3K/Akt signaling. ► LY294002 suppresses HDGF-mediated cytoskeleton...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2012-08, Vol.425 (2), p.169-176 |
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| creator | Kung, Mei-Lang Tsai, Han-En Hu, Tsung-Hui Kuo, Hsiao-Mei Liu, Li-Fen Chen, San-Cher Lin, Pey-Ru Ma, Yi-Ling Wang, E-Ming Liu, Guei-Sheung Liu, Jong-Kang Tai, Ming-Hong |
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► HDGF stimulates podosome formation, dorsal ruffles formation and cell migration. ► PI3K/Akt signaling cascade is involved in the HDGF-mediated cell migration. ► Ad-PTENT inhibits HDGF-mediated cell migration and PI3K/Akt signaling. ► LY294002 suppresses HDGF-mediated cytoskeleton reorganization and migration.
Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration. |
| doi_str_mv | 10.1016/j.bbrc.2012.07.060 |
| format | Article |
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► HDGF stimulates podosome formation, dorsal ruffles formation and cell migration. ► PI3K/Akt signaling cascade is involved in the HDGF-mediated cell migration. ► Ad-PTENT inhibits HDGF-mediated cell migration and PI3K/Akt signaling. ► LY294002 suppresses HDGF-mediated cytoskeleton reorganization and migration.
Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2012.07.060</identifier><identifier>PMID: 22819846</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Akt ; Animals ; Cell migration ; Cell Movement ; Cytoskeleton - genetics ; Cytoskeleton - metabolism ; Enzyme Activation ; Hepatoma-derived growth factor ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - physiology ; Mice ; NIH 3T3 Cells ; Phosphatidylinositol 3-Kinase - biosynthesis ; PI3K ; Podosome ; Proto-Oncogene Proteins c-akt - biosynthesis ; Rosette Formation ; Transfection</subject><ispartof>Biochemical and biophysical research communications, 2012-08, Vol.425 (2), p.169-176</ispartof><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-81eeac144f054beee1e921541f71545c4fa575a016db93a19e573d6c8602e743</citedby><cites>FETCH-LOGICAL-c422t-81eeac144f054beee1e921541f71545c4fa575a016db93a19e573d6c8602e743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X12013563$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22819846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kung, Mei-Lang</creatorcontrib><creatorcontrib>Tsai, Han-En</creatorcontrib><creatorcontrib>Hu, Tsung-Hui</creatorcontrib><creatorcontrib>Kuo, Hsiao-Mei</creatorcontrib><creatorcontrib>Liu, Li-Fen</creatorcontrib><creatorcontrib>Chen, San-Cher</creatorcontrib><creatorcontrib>Lin, Pey-Ru</creatorcontrib><creatorcontrib>Ma, Yi-Ling</creatorcontrib><creatorcontrib>Wang, E-Ming</creatorcontrib><creatorcontrib>Liu, Guei-Sheung</creatorcontrib><creatorcontrib>Liu, Jong-Kang</creatorcontrib><creatorcontrib>Tai, Ming-Hong</creatorcontrib><title>Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>[Display omitted]
► HDGF stimulates podosome formation, dorsal ruffles formation and cell migration. ► PI3K/Akt signaling cascade is involved in the HDGF-mediated cell migration. ► Ad-PTENT inhibits HDGF-mediated cell migration and PI3K/Akt signaling. ► LY294002 suppresses HDGF-mediated cytoskeleton reorganization and migration.
Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.</description><subject>Akt</subject><subject>Animals</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Cytoskeleton - genetics</subject><subject>Cytoskeleton - metabolism</subject><subject>Enzyme Activation</subject><subject>Hepatoma-derived growth factor</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Intercellular Signaling Peptides and Proteins - physiology</subject><subject>Mice</subject><subject>NIH 3T3 Cells</subject><subject>Phosphatidylinositol 3-Kinase - biosynthesis</subject><subject>PI3K</subject><subject>Podosome</subject><subject>Proto-Oncogene Proteins c-akt - biosynthesis</subject><subject>Rosette Formation</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhBTggH7kkO3acZCNxqarCVqrgsgdulmNPGm-TONjOVvsqPC2OtnDk4pGtb3555iPkI4OcAau2x7xtvc45MJ5DnUMFr8iGQQMZZyBekw0AVBlv2M8r8i6EIwBjomrekivOd6zZiWpDfu9xVtGNKjPo7QkNffTuOfa0Uzo6T0O04zKoiIHOzrjgRqTeBYzrS-f8qKJ1E7UT_X6_3xaHgmochkBj793y2KeKNCXZ04VzHZ17F-Y-Xc15sJMLNrqBFtmTnVTA7c1TpOlD_bM6vydvOjUE_PBSr8nh693hdp89_Ph2f3vzkGnBecx2DFFpJkQHpWgRkWHDWSlYV6ez1KJTZV2qtDDTNoViDZZ1YSq9q4BjLYpr8vkSO3v3a8EQ5WjDOoSa0C1BMijKkjccqoTyC6rTCoLHTs7ejsqfEyRXJfIoVyVyVSKhlklJavr0kr-0I5p_LX8dJODLBcA05Mmil0FbnDQa61FHaZz9X_4frSqgOQ</recordid><startdate>20120824</startdate><enddate>20120824</enddate><creator>Kung, Mei-Lang</creator><creator>Tsai, Han-En</creator><creator>Hu, Tsung-Hui</creator><creator>Kuo, Hsiao-Mei</creator><creator>Liu, Li-Fen</creator><creator>Chen, San-Cher</creator><creator>Lin, Pey-Ru</creator><creator>Ma, Yi-Ling</creator><creator>Wang, E-Ming</creator><creator>Liu, Guei-Sheung</creator><creator>Liu, Jong-Kang</creator><creator>Tai, Ming-Hong</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120824</creationdate><title>Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway</title><author>Kung, Mei-Lang ; Tsai, Han-En ; Hu, Tsung-Hui ; Kuo, Hsiao-Mei ; Liu, Li-Fen ; Chen, San-Cher ; Lin, Pey-Ru ; Ma, Yi-Ling ; Wang, E-Ming ; Liu, Guei-Sheung ; Liu, Jong-Kang ; Tai, Ming-Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-81eeac144f054beee1e921541f71545c4fa575a016db93a19e573d6c8602e743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Akt</topic><topic>Animals</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Cytoskeleton - genetics</topic><topic>Cytoskeleton - metabolism</topic><topic>Enzyme Activation</topic><topic>Hepatoma-derived growth factor</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Intercellular Signaling Peptides and Proteins - physiology</topic><topic>Mice</topic><topic>NIH 3T3 Cells</topic><topic>Phosphatidylinositol 3-Kinase - biosynthesis</topic><topic>PI3K</topic><topic>Podosome</topic><topic>Proto-Oncogene Proteins c-akt - biosynthesis</topic><topic>Rosette Formation</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kung, Mei-Lang</creatorcontrib><creatorcontrib>Tsai, Han-En</creatorcontrib><creatorcontrib>Hu, Tsung-Hui</creatorcontrib><creatorcontrib>Kuo, Hsiao-Mei</creatorcontrib><creatorcontrib>Liu, Li-Fen</creatorcontrib><creatorcontrib>Chen, San-Cher</creatorcontrib><creatorcontrib>Lin, Pey-Ru</creatorcontrib><creatorcontrib>Ma, Yi-Ling</creatorcontrib><creatorcontrib>Wang, E-Ming</creatorcontrib><creatorcontrib>Liu, Guei-Sheung</creatorcontrib><creatorcontrib>Liu, Jong-Kang</creatorcontrib><creatorcontrib>Tai, Ming-Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kung, Mei-Lang</au><au>Tsai, Han-En</au><au>Hu, Tsung-Hui</au><au>Kuo, Hsiao-Mei</au><au>Liu, Li-Fen</au><au>Chen, San-Cher</au><au>Lin, Pey-Ru</au><au>Ma, Yi-Ling</au><au>Wang, E-Ming</au><au>Liu, Guei-Sheung</au><au>Liu, Jong-Kang</au><au>Tai, Ming-Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2012-08-24</date><risdate>2012</risdate><volume>425</volume><issue>2</issue><spage>169</spage><epage>176</epage><pages>169-176</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>[Display omitted]
► HDGF stimulates podosome formation, dorsal ruffles formation and cell migration. ► PI3K/Akt signaling cascade is involved in the HDGF-mediated cell migration. ► Ad-PTENT inhibits HDGF-mediated cell migration and PI3K/Akt signaling. ► LY294002 suppresses HDGF-mediated cytoskeleton reorganization and migration.
Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22819846</pmid><doi>10.1016/j.bbrc.2012.07.060</doi><tpages>8</tpages></addata></record> |
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| subjects | Akt Animals Cell migration Cell Movement Cytoskeleton - genetics Cytoskeleton - metabolism Enzyme Activation Hepatoma-derived growth factor Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - physiology Mice NIH 3T3 Cells Phosphatidylinositol 3-Kinase - biosynthesis PI3K Podosome Proto-Oncogene Proteins c-akt - biosynthesis Rosette Formation Transfection |
| title | Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway |
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