Hypothalamic transcript profiling in hypothermia using SuperSAGE
Abstract Understanding of molecular mechanisms underlying hypothermia is of primary importance in devising strategies to diagnose hypothermia. We investigated the hypothalamic transriptome in hypothermia. For transcriptomic analyses, SuperSAGE, an improved method of serial analysis of gene expressio...
Gespeichert in:
| Veröffentlicht in: | Journal of forensic and legal medicine 2012-10, Vol.19 (7), p.396-401 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 401 |
|---|---|
| container_issue | 7 |
| container_start_page | 396 |
| container_title | Journal of forensic and legal medicine |
| container_volume | 19 |
| creator | Takamiya, Masataka, MD, PhD Nakayashiki, Nori, PhD Dewa, Koji, MD, PhD |
| description | Abstract Understanding of molecular mechanisms underlying hypothermia is of primary importance in devising strategies to diagnose hypothermia. We investigated the hypothalamic transriptome in hypothermia. For transcriptomic analyses, SuperSAGE, an improved method of serial analysis of gene expression, was used. Totally, 62,208 and 54,084 tags were collected from the hypothalami of normal and hypothermia, respectively. And 367 transcripts were differentially expressed at a statistically significant level. That is, 157 and 210 transcripts among them were expressed at a higher level in normal and hypothermic hypothalami. Results obtained by SuperSAGE and quantitative PCR were consistent in 6 selected genes, although levels of differences detected by the 2 methods were not exactly the same. mRNA expressions in the hypothalamus were considered to be useful for hypothermic diagnosis. Various methods have been applied for gene expression analyses and biomarker detections. However in forensic pathology, SuperSAGE would be a promising method, especially in gene discoveries and transcriptomic analyses. |
| doi_str_mv | 10.1016/j.jflm.2012.04.017 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1035528656</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S1752928X1200090X</els_id><sourcerecordid>2749200741</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-9a1fe8ac7caf41a528902b2a33bf5c39afe158fd0a62a6c8fe519650133e65d83</originalsourceid><addsrcrecordid>eNp9kU1rFTEUhoMotlb_gAsZcONmxpOvSQZELKW2QsHFVegu5GZObMb5MpkR7r83460KXbhKCM_7cvIcQl5SqCjQ-m1Xdb4fKgaUVSAqoOoROaVa6VIJrR7nu5KsbJi-PSHPUuoApGBKPiUnjDUMVM1OyYfrwzwtd7a3Q3DFEu2YXAzzUsxx8qEP47cijMXdbwjjEGyxpu1xt84Yd-dXl8_JE2_7hC_uzzPy9ePll4vr8ubz1aeL85vSCd4sZWOpR22dctYLaiXTDbA9s5zvvXS8sR6p1L4FWzNbO-1R0qaWQDnHWraan5E3x9482I8V02KGkBz2vR1xWpOhwGVurWWd0dcP0G5a45in2yjFBRNSZIodKRenlCJ6M8cw2HjIkNn8ms5sfs3m14Aw2W8OvbqvXvcDtn8jf4Rm4N0RwOziZ8Bokgs4OmxDRLeYdgr_73__IO7yDoKz_Xc8YPr3D5Nyxuy2DW8LpgwAGrjlvwBqyqBY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1037342454</pqid></control><display><type>article</type><title>Hypothalamic transcript profiling in hypothermia using SuperSAGE</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Takamiya, Masataka, MD, PhD ; Nakayashiki, Nori, PhD ; Dewa, Koji, MD, PhD</creator><creatorcontrib>Takamiya, Masataka, MD, PhD ; Nakayashiki, Nori, PhD ; Dewa, Koji, MD, PhD</creatorcontrib><description>Abstract Understanding of molecular mechanisms underlying hypothermia is of primary importance in devising strategies to diagnose hypothermia. We investigated the hypothalamic transriptome in hypothermia. For transcriptomic analyses, SuperSAGE, an improved method of serial analysis of gene expression, was used. Totally, 62,208 and 54,084 tags were collected from the hypothalami of normal and hypothermia, respectively. And 367 transcripts were differentially expressed at a statistically significant level. That is, 157 and 210 transcripts among them were expressed at a higher level in normal and hypothermic hypothalami. Results obtained by SuperSAGE and quantitative PCR were consistent in 6 selected genes, although levels of differences detected by the 2 methods were not exactly the same. mRNA expressions in the hypothalamus were considered to be useful for hypothermic diagnosis. Various methods have been applied for gene expression analyses and biomarker detections. However in forensic pathology, SuperSAGE would be a promising method, especially in gene discoveries and transcriptomic analyses.</description><identifier>ISSN: 1752-928X</identifier><identifier>EISSN: 1878-7487</identifier><identifier>DOI: 10.1016/j.jflm.2012.04.017</identifier><identifier>PMID: 22920762</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Biomarkers ; Calmodulin-Binding Proteins - genetics ; Calmodulin-Binding Proteins - metabolism ; Chromosomal Proteins, Non-Histone - genetics ; Chromosomal Proteins, Non-Histone - metabolism ; Dopamine Plasma Membrane Transport Proteins - genetics ; Dopamine Plasma Membrane Transport Proteins - metabolism ; Expressed Sequence Tags ; Fever ; Forensic Genetics ; Forensic Pathology ; Gene expression ; Gene Expression Profiling ; Genetic testing ; HSC70 Heat-Shock Proteins - genetics ; HSC70 Heat-Shock Proteins - metabolism ; Hypothalamus ; Hypothalamus - metabolism ; Hypothermia ; Hypothermia - diagnosis ; Male ; Medical diagnosis ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Oligonucleotide Array Sequence Analysis - methods ; Pathology ; Polymerase Chain Reaction ; Quantitative PCR ; Rats ; Rats, Wistar ; RNA, Messenger - metabolism ; SuperSAGE ; Transcriptome ; Tryptophan Hydroxylase - genetics ; Tryptophan Hydroxylase - metabolism ; Tyrosine 3-Monooxygenase - genetics ; Tyrosine 3-Monooxygenase - metabolism</subject><ispartof>Journal of forensic and legal medicine, 2012-10, Vol.19 (7), p.396-401</ispartof><rights>Elsevier Ltd and Faculty of Forensic and Legal Medicine</rights><rights>2012 Elsevier Ltd and Faculty of Forensic and Legal Medicine</rights><rights>Copyright © 2012 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.</rights><rights>Copyright Churchill Livingstone Inc., Medical Publishers Oct 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-9a1fe8ac7caf41a528902b2a33bf5c39afe158fd0a62a6c8fe519650133e65d83</citedby><cites>FETCH-LOGICAL-c439t-9a1fe8ac7caf41a528902b2a33bf5c39afe158fd0a62a6c8fe519650133e65d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jflm.2012.04.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22920762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takamiya, Masataka, MD, PhD</creatorcontrib><creatorcontrib>Nakayashiki, Nori, PhD</creatorcontrib><creatorcontrib>Dewa, Koji, MD, PhD</creatorcontrib><title>Hypothalamic transcript profiling in hypothermia using SuperSAGE</title><title>Journal of forensic and legal medicine</title><addtitle>J Forensic Leg Med</addtitle><description>Abstract Understanding of molecular mechanisms underlying hypothermia is of primary importance in devising strategies to diagnose hypothermia. We investigated the hypothalamic transriptome in hypothermia. For transcriptomic analyses, SuperSAGE, an improved method of serial analysis of gene expression, was used. Totally, 62,208 and 54,084 tags were collected from the hypothalami of normal and hypothermia, respectively. And 367 transcripts were differentially expressed at a statistically significant level. That is, 157 and 210 transcripts among them were expressed at a higher level in normal and hypothermic hypothalami. Results obtained by SuperSAGE and quantitative PCR were consistent in 6 selected genes, although levels of differences detected by the 2 methods were not exactly the same. mRNA expressions in the hypothalamus were considered to be useful for hypothermic diagnosis. Various methods have been applied for gene expression analyses and biomarker detections. However in forensic pathology, SuperSAGE would be a promising method, especially in gene discoveries and transcriptomic analyses.</description><subject>Animals</subject><subject>Biomarkers</subject><subject>Calmodulin-Binding Proteins - genetics</subject><subject>Calmodulin-Binding Proteins - metabolism</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Dopamine Plasma Membrane Transport Proteins - genetics</subject><subject>Dopamine Plasma Membrane Transport Proteins - metabolism</subject><subject>Expressed Sequence Tags</subject><subject>Fever</subject><subject>Forensic Genetics</subject><subject>Forensic Pathology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genetic testing</subject><subject>HSC70 Heat-Shock Proteins - genetics</subject><subject>HSC70 Heat-Shock Proteins - metabolism</subject><subject>Hypothalamus</subject><subject>Hypothalamus - metabolism</subject><subject>Hypothermia</subject><subject>Hypothermia - diagnosis</subject><subject>Male</subject><subject>Medical diagnosis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Pathology</subject><subject>Polymerase Chain Reaction</subject><subject>Quantitative PCR</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - metabolism</subject><subject>SuperSAGE</subject><subject>Transcriptome</subject><subject>Tryptophan Hydroxylase - genetics</subject><subject>Tryptophan Hydroxylase - metabolism</subject><subject>Tyrosine 3-Monooxygenase - genetics</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><issn>1752-928X</issn><issn>1878-7487</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rFTEUhoMotlb_gAsZcONmxpOvSQZELKW2QsHFVegu5GZObMb5MpkR7r83460KXbhKCM_7cvIcQl5SqCjQ-m1Xdb4fKgaUVSAqoOoROaVa6VIJrR7nu5KsbJi-PSHPUuoApGBKPiUnjDUMVM1OyYfrwzwtd7a3Q3DFEu2YXAzzUsxx8qEP47cijMXdbwjjEGyxpu1xt84Yd-dXl8_JE2_7hC_uzzPy9ePll4vr8ubz1aeL85vSCd4sZWOpR22dctYLaiXTDbA9s5zvvXS8sR6p1L4FWzNbO-1R0qaWQDnHWraan5E3x9482I8V02KGkBz2vR1xWpOhwGVurWWd0dcP0G5a45in2yjFBRNSZIodKRenlCJ6M8cw2HjIkNn8ms5sfs3m14Aw2W8OvbqvXvcDtn8jf4Rm4N0RwOziZ8Bokgs4OmxDRLeYdgr_73__IO7yDoKz_Xc8YPr3D5Nyxuy2DW8LpgwAGrjlvwBqyqBY</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Takamiya, Masataka, MD, PhD</creator><creator>Nakayashiki, Nori, PhD</creator><creator>Dewa, Koji, MD, PhD</creator><general>Elsevier Ltd</general><general>Churchill Livingstone Inc., Medical Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121001</creationdate><title>Hypothalamic transcript profiling in hypothermia using SuperSAGE</title><author>Takamiya, Masataka, MD, PhD ; Nakayashiki, Nori, PhD ; Dewa, Koji, MD, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-9a1fe8ac7caf41a528902b2a33bf5c39afe158fd0a62a6c8fe519650133e65d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biomarkers</topic><topic>Calmodulin-Binding Proteins - genetics</topic><topic>Calmodulin-Binding Proteins - metabolism</topic><topic>Chromosomal Proteins, Non-Histone - genetics</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>Dopamine Plasma Membrane Transport Proteins - genetics</topic><topic>Dopamine Plasma Membrane Transport Proteins - metabolism</topic><topic>Expressed Sequence Tags</topic><topic>Fever</topic><topic>Forensic Genetics</topic><topic>Forensic Pathology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Genetic testing</topic><topic>HSC70 Heat-Shock Proteins - genetics</topic><topic>HSC70 Heat-Shock Proteins - metabolism</topic><topic>Hypothalamus</topic><topic>Hypothalamus - metabolism</topic><topic>Hypothermia</topic><topic>Hypothermia - diagnosis</topic><topic>Male</topic><topic>Medical diagnosis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Pathology</topic><topic>Polymerase Chain Reaction</topic><topic>Quantitative PCR</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - metabolism</topic><topic>SuperSAGE</topic><topic>Transcriptome</topic><topic>Tryptophan Hydroxylase - genetics</topic><topic>Tryptophan Hydroxylase - metabolism</topic><topic>Tyrosine 3-Monooxygenase - genetics</topic><topic>Tyrosine 3-Monooxygenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takamiya, Masataka, MD, PhD</creatorcontrib><creatorcontrib>Nakayashiki, Nori, PhD</creatorcontrib><creatorcontrib>Dewa, Koji, MD, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of forensic and legal medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takamiya, Masataka, MD, PhD</au><au>Nakayashiki, Nori, PhD</au><au>Dewa, Koji, MD, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypothalamic transcript profiling in hypothermia using SuperSAGE</atitle><jtitle>Journal of forensic and legal medicine</jtitle><addtitle>J Forensic Leg Med</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>19</volume><issue>7</issue><spage>396</spage><epage>401</epage><pages>396-401</pages><issn>1752-928X</issn><eissn>1878-7487</eissn><abstract>Abstract Understanding of molecular mechanisms underlying hypothermia is of primary importance in devising strategies to diagnose hypothermia. We investigated the hypothalamic transriptome in hypothermia. For transcriptomic analyses, SuperSAGE, an improved method of serial analysis of gene expression, was used. Totally, 62,208 and 54,084 tags were collected from the hypothalami of normal and hypothermia, respectively. And 367 transcripts were differentially expressed at a statistically significant level. That is, 157 and 210 transcripts among them were expressed at a higher level in normal and hypothermic hypothalami. Results obtained by SuperSAGE and quantitative PCR were consistent in 6 selected genes, although levels of differences detected by the 2 methods were not exactly the same. mRNA expressions in the hypothalamus were considered to be useful for hypothermic diagnosis. Various methods have been applied for gene expression analyses and biomarker detections. However in forensic pathology, SuperSAGE would be a promising method, especially in gene discoveries and transcriptomic analyses.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22920762</pmid><doi>10.1016/j.jflm.2012.04.017</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1752-928X |
| ispartof | Journal of forensic and legal medicine, 2012-10, Vol.19 (7), p.396-401 |
| issn | 1752-928X 1878-7487 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1035528656 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biomarkers Calmodulin-Binding Proteins - genetics Calmodulin-Binding Proteins - metabolism Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism Dopamine Plasma Membrane Transport Proteins - genetics Dopamine Plasma Membrane Transport Proteins - metabolism Expressed Sequence Tags Fever Forensic Genetics Forensic Pathology Gene expression Gene Expression Profiling Genetic testing HSC70 Heat-Shock Proteins - genetics HSC70 Heat-Shock Proteins - metabolism Hypothalamus Hypothalamus - metabolism Hypothermia Hypothermia - diagnosis Male Medical diagnosis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Oligonucleotide Array Sequence Analysis - methods Pathology Polymerase Chain Reaction Quantitative PCR Rats Rats, Wistar RNA, Messenger - metabolism SuperSAGE Transcriptome Tryptophan Hydroxylase - genetics Tryptophan Hydroxylase - metabolism Tyrosine 3-Monooxygenase - genetics Tyrosine 3-Monooxygenase - metabolism |
| title | Hypothalamic transcript profiling in hypothermia using SuperSAGE |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T00%3A27%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hypothalamic%20transcript%20profiling%20in%20hypothermia%20using%20SuperSAGE&rft.jtitle=Journal%20of%20forensic%20and%20legal%20medicine&rft.au=Takamiya,%20Masataka,%20MD,%20PhD&rft.date=2012-10-01&rft.volume=19&rft.issue=7&rft.spage=396&rft.epage=401&rft.pages=396-401&rft.issn=1752-928X&rft.eissn=1878-7487&rft_id=info:doi/10.1016/j.jflm.2012.04.017&rft_dat=%3Cproquest_cross%3E2749200741%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1037342454&rft_id=info:pmid/22920762&rft_els_id=1_s2_0_S1752928X1200090X&rfr_iscdi=true |