A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver
The intranuclear disposition of plasmid DNA is extremely important for transgene expression. The interactions between the plasmid DNA and the histone proteins are one of the keys for controlling the disposition. In this study, the effects of a left-handedly curved sequence (20–40 repeated A•T tracts...
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| creator | Fukunaga, S Kanda, G Tanase, J Harashima, H Ohyama, T Kamiya, H |
| description | The intranuclear disposition of plasmid DNA is extremely important for transgene expression. The interactions between the plasmid DNA and the histone proteins are one of the keys for controlling the disposition. In this study, the effects of a left-handedly curved sequence (20–40 repeated A•T tracts) on transgene expression from a plasmid were examined
in vivo
. A naked luciferase plasmid with the curved sequence was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. Interestingly, transgene expression was markedly increased by the addition of the curved sequence. An analysis of the nucleosome positions near the left-handedly curved sequence suggested that the sequence functions as an acceptor of the histone core and allows nucleosome sliding, resulting in transcriptional activation. These results suggested that the designed curved DNA sequences could control transgene expression from plasmid DNAs
in vivo
. |
| doi_str_mv | 10.1038/gt.2011.127 |
| format | Article |
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in vivo
. A naked luciferase plasmid with the curved sequence was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. Interestingly, transgene expression was markedly increased by the addition of the curved sequence. An analysis of the nucleosome positions near the left-handedly curved sequence suggested that the sequence functions as an acceptor of the histone core and allows nucleosome sliding, resulting in transcriptional activation. These results suggested that the designed curved DNA sequences could control transgene expression from plasmid DNAs
in vivo
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in vivo
. A naked luciferase plasmid with the curved sequence was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. Interestingly, transgene expression was markedly increased by the addition of the curved sequence. An analysis of the nucleosome positions near the left-handedly curved sequence suggested that the sequence functions as an acceptor of the histone core and allows nucleosome sliding, resulting in transcriptional activation. These results suggested that the designed curved DNA sequences could control transgene expression from plasmid DNAs
in vivo
.</description><subject>631/1647/1511</subject><subject>631/208/199</subject><subject>631/337/100/1701</subject><subject>Analysis</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Applied cell therapy and gene therapy</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Cell Nucleus - metabolism</subject><subject>Chromatin. Chromosome</subject><subject>Deoxyribonucleic acid</subject><subject>Disposition</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Therapy</subject><subject>Genetic aspects</subject><subject>Handedness</subject><subject>Health. Pharmaceutical industry</subject><subject>Histones</subject><subject>Histones - metabolism</subject><subject>Human Genetics</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Keys</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nanotechnology</subject><subject>Nucleosomes</subject><subject>Nucleosomes - genetics</subject><subject>Nucleotide sequence</subject><subject>original-article</subject><subject>Physiological aspects</subject><subject>Plasmids</subject><subject>Plasmids - genetics</subject><subject>Rodents</subject><subject>Transcription activation</subject><subject>Transfusions. Complications. Transfusion reactions. 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Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Applied cell therapy and gene therapy</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Cell Nucleus - metabolism</topic><topic>Chromatin. Chromosome</topic><topic>Deoxyribonucleic acid</topic><topic>Disposition</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Therapy</topic><topic>Genetic aspects</topic><topic>Handedness</topic><topic>Health. Pharmaceutical industry</topic><topic>Histones</topic><topic>Histones - metabolism</topic><topic>Human Genetics</topic><topic>Industrial applications and implications. 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The interactions between the plasmid DNA and the histone proteins are one of the keys for controlling the disposition. In this study, the effects of a left-handedly curved sequence (20–40 repeated A•T tracts) on transgene expression from a plasmid were examined
in vivo
. A naked luciferase plasmid with the curved sequence was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. Interestingly, transgene expression was markedly increased by the addition of the curved sequence. An analysis of the nucleosome positions near the left-handedly curved sequence suggested that the sequence functions as an acceptor of the histone core and allows nucleosome sliding, resulting in transcriptional activation. These results suggested that the designed curved DNA sequences could control transgene expression from plasmid DNAs
in vivo
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>21918548</pmid><doi>10.1038/gt.2011.127</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Gene therapy, 2012-08, Vol.19 (8), p.828-835 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; SpringerLink Journals - AutoHoldings |
| subjects | 631/1647/1511 631/208/199 631/337/100/1701 Analysis Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Applied cell therapy and gene therapy Base Sequence Biological and medical sciences Biomedical and Life Sciences Biomedicine Biotechnology Cell Biology Cell Nucleus - metabolism Chromatin. Chromosome Deoxyribonucleic acid Disposition DNA Fundamental and applied biological sciences. Psychology Gene Expression Gene Therapy Genetic aspects Handedness Health. Pharmaceutical industry Histones Histones - metabolism Human Genetics Industrial applications and implications. Economical aspects Keys Liver Liver - metabolism Male Medical sciences Mice Mice, Inbred BALB C Molecular and cellular biology Molecular genetics Nanotechnology Nucleosomes Nucleosomes - genetics Nucleotide sequence original-article Physiological aspects Plasmids Plasmids - genetics Rodents Transcription activation Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transgenes |
| title | A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver |
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