Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes
Listeria monocytogenes (LM) is a major foodborne pathogen causing septicemia, meningitis and death in humans. LM infection is preceded by its attachment to and invasion of human intestinal epithelium followed by systemic spread. The major virulence factors in LM include motility, hemolysin and lecit...
Gespeichert in:
| Veröffentlicht in: | International journal of food microbiology 2012-06, Vol.157 (1), p.88-94 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 94 |
|---|---|
| container_issue | 1 |
| container_start_page | 88 |
| container_title | International journal of food microbiology |
| container_volume | 157 |
| creator | Upadhyay, Abhinav Johny, Anup Kollanoor Amalaradjou, Mary Anne Roshni Ananda Baskaran, Sangeetha Kim, Kwang Sik Venkitanarayanan, Kumar |
| description | Listeria monocytogenes (LM) is a major foodborne pathogen causing septicemia, meningitis and death in humans. LM infection is preceded by its attachment to and invasion of human intestinal epithelium followed by systemic spread. The major virulence factors in LM include motility, hemolysin and lecithinase production. Reducing LM attachment to and invasion of host tissue and production of virulence factors could potentially control listeriosis in humans. This study investigated the efficacy of sub-inhibitory concentrations (SICs, concentrations not inhibiting bacterial growth) of three, generally regarded as safe (GRAS)-status, plant-derived antimicrobial compounds in reducing LM attachment to and invasion of human colon adenocarcinoma (Caco-2) and human brain microvascular endothelial cells (HBMEC). Additionally, the effect of these compounds on the aforementioned LM virulence factors was studied. The compounds and their respective SICs used relative to their MICs were trans-cinnamaldehyde (TC 0.50mM, 0.75mM with the MIC of 0.90mM), carvacrol (CR 0.50mM, 0.65mM with the MIC of 0.75mM), and thymol (TY 0.33mM, 0.50mM with the MIC of 0.60mM). All three-plant antimicrobials reduced LM adhesion to and invasion of Caco-2 and HBMEC (p |
| doi_str_mv | 10.1016/j.ijfoodmicro.2012.04.018 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1034822915</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S016816051200222X</els_id><sourcerecordid>1034822915</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-d17a38efd28556cf75f3fd77d377291d2254329527c6d310e4ea809a5c2e1d63</originalsourceid><addsrcrecordid>eNqNkc1u1DAUhS1ERYfCK4DZdUGCf-I4WaIRBaSRikRZW659PfIoiQc7mdIX4Lm50ymoS1a-sr5z7tU5hLzjrOaMtx92ddyFlPwYXU61YFzUrKkZ756RFe90X8mmZc_JCtmu4i1T5-RlKTvGmJKSvSDnQrSsa5Vekd_fBjvNlYccD-ApzvHB9TbaodAMfnFAN7HMCFg6pim5-zltYYJCDzEvA0wIBOvmlAuNE37OOb1HI099upuqDNtlsDNQ-LXPUEpME03hifbB6xU5C7gQXj--F-Tm6tPN-ku1uf78df1xU7lGNngm11Z2ELzolGpd0CrI4LX2UmvRcy-EaqToldCu9ZIzaMB2rLfKCeC-lRfk8mS7z-nnAmU2YywOBswA0lIMZ7LpBDopRPsTimGUkiGYfY6jzfcImWMLZmeetGCOLRjWGGwBtW8e1yy3I_h_yr-xI_D2BASbjN3mWMyP7-jQMJT3WCES6xMBmMYhQjbFxWNePmZws_Ep_schfwD9I6tV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1034822915</pqid></control><display><type>article</type><title>Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Upadhyay, Abhinav ; Johny, Anup Kollanoor ; Amalaradjou, Mary Anne Roshni ; Ananda Baskaran, Sangeetha ; Kim, Kwang Sik ; Venkitanarayanan, Kumar</creator><creatorcontrib>Upadhyay, Abhinav ; Johny, Anup Kollanoor ; Amalaradjou, Mary Anne Roshni ; Ananda Baskaran, Sangeetha ; Kim, Kwang Sik ; Venkitanarayanan, Kumar</creatorcontrib><description>Listeria monocytogenes (LM) is a major foodborne pathogen causing septicemia, meningitis and death in humans. LM infection is preceded by its attachment to and invasion of human intestinal epithelium followed by systemic spread. The major virulence factors in LM include motility, hemolysin and lecithinase production. Reducing LM attachment to and invasion of host tissue and production of virulence factors could potentially control listeriosis in humans. This study investigated the efficacy of sub-inhibitory concentrations (SICs, concentrations not inhibiting bacterial growth) of three, generally regarded as safe (GRAS)-status, plant-derived antimicrobial compounds in reducing LM attachment to and invasion of human colon adenocarcinoma (Caco-2) and human brain microvascular endothelial cells (HBMEC). Additionally, the effect of these compounds on the aforementioned LM virulence factors was studied. The compounds and their respective SICs used relative to their MICs were trans-cinnamaldehyde (TC 0.50mM, 0.75mM with the MIC of 0.90mM), carvacrol (CR 0.50mM, 0.65mM with the MIC of 0.75mM), and thymol (TY 0.33mM, 0.50mM with the MIC of 0.60mM). All three-plant antimicrobials reduced LM adhesion to and invasion of Caco-2 and HBMEC (p<0.05). The compounds also decreased LM motility, hemolysin production and lecithinase activity (p<0.05). Real-time PCR data revealed that TC, CR, and TY down-regulated the expression of LM virulence genes by >3.0 folds compared to controls (p<0.05). Results suggest that TC, CR, and TY could potentially be used to control LM infection; however, in vivo studies are necessary to validate these results.
► SICs of Trans-cinnamaldehyde (TC), Carvacrol (CR), and Thymol (TY) reduced motility, hemolysin and lecithinase production. ► SICs of TC, CR, and TY completely shut down LM invasion on HBMEC and reduced adhesion and invasion on Caco-2 cells significantly. ► SICs of TC, CR, and TY down regulated expression of major virulence genes.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2012.04.018</identifier><identifier>PMID: 22608657</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adenocarcinoma ; adhesion ; Anti-Infective Agents - pharmacology ; Antimicrobial agents ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Proteins - physiology ; Brain ; Brain - cytology ; Caco-2 Cells ; Carvacrol ; Cell Culture Techniques ; Colon ; death ; disease control ; Down-Regulation ; Endothelial Cells ; Epithelium ; Food ; food pathogens ; Foodborne Diseases - prevention & control ; gene expression ; Gene regulation ; genes ; GRAS substances ; Hemolysin Proteins - genetics ; Hemolysin Proteins - metabolism ; Hemolysins ; hosts ; Humans ; in vivo studies ; Infection ; intestinal mucosa ; Intestine ; Lecithinase ; Listeria monocytogenes ; Listeria monocytogenes - genetics ; Listeria monocytogenes - pathogenicity ; Listeria monocytogenes - physiology ; Listeriosis ; Listeriosis - prevention & control ; Meningitis ; microbial growth ; Microvasculature ; Minimum inhibitory concentration ; Motility ; Pathogens ; Phospholipases - metabolism ; Plant compounds ; Plant Extracts - pharmacology ; Plants ; polymerase chain reaction ; Septicemia ; thymol ; Virulence ; Virulence - genetics ; virulence factors ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>International journal of food microbiology, 2012-06, Vol.157 (1), p.88-94</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-d17a38efd28556cf75f3fd77d377291d2254329527c6d310e4ea809a5c2e1d63</citedby><cites>FETCH-LOGICAL-c434t-d17a38efd28556cf75f3fd77d377291d2254329527c6d310e4ea809a5c2e1d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2012.04.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22608657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Upadhyay, Abhinav</creatorcontrib><creatorcontrib>Johny, Anup Kollanoor</creatorcontrib><creatorcontrib>Amalaradjou, Mary Anne Roshni</creatorcontrib><creatorcontrib>Ananda Baskaran, Sangeetha</creatorcontrib><creatorcontrib>Kim, Kwang Sik</creatorcontrib><creatorcontrib>Venkitanarayanan, Kumar</creatorcontrib><title>Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Listeria monocytogenes (LM) is a major foodborne pathogen causing septicemia, meningitis and death in humans. LM infection is preceded by its attachment to and invasion of human intestinal epithelium followed by systemic spread. The major virulence factors in LM include motility, hemolysin and lecithinase production. Reducing LM attachment to and invasion of host tissue and production of virulence factors could potentially control listeriosis in humans. This study investigated the efficacy of sub-inhibitory concentrations (SICs, concentrations not inhibiting bacterial growth) of three, generally regarded as safe (GRAS)-status, plant-derived antimicrobial compounds in reducing LM attachment to and invasion of human colon adenocarcinoma (Caco-2) and human brain microvascular endothelial cells (HBMEC). Additionally, the effect of these compounds on the aforementioned LM virulence factors was studied. The compounds and their respective SICs used relative to their MICs were trans-cinnamaldehyde (TC 0.50mM, 0.75mM with the MIC of 0.90mM), carvacrol (CR 0.50mM, 0.65mM with the MIC of 0.75mM), and thymol (TY 0.33mM, 0.50mM with the MIC of 0.60mM). All three-plant antimicrobials reduced LM adhesion to and invasion of Caco-2 and HBMEC (p<0.05). The compounds also decreased LM motility, hemolysin production and lecithinase activity (p<0.05). Real-time PCR data revealed that TC, CR, and TY down-regulated the expression of LM virulence genes by >3.0 folds compared to controls (p<0.05). Results suggest that TC, CR, and TY could potentially be used to control LM infection; however, in vivo studies are necessary to validate these results.
► SICs of Trans-cinnamaldehyde (TC), Carvacrol (CR), and Thymol (TY) reduced motility, hemolysin and lecithinase production. ► SICs of TC, CR, and TY completely shut down LM invasion on HBMEC and reduced adhesion and invasion on Caco-2 cells significantly. ► SICs of TC, CR, and TY down regulated expression of major virulence genes.</description><subject>Adenocarcinoma</subject><subject>adhesion</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Antimicrobial agents</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Proteins - physiology</subject><subject>Brain</subject><subject>Brain - cytology</subject><subject>Caco-2 Cells</subject><subject>Carvacrol</subject><subject>Cell Culture Techniques</subject><subject>Colon</subject><subject>death</subject><subject>disease control</subject><subject>Down-Regulation</subject><subject>Endothelial Cells</subject><subject>Epithelium</subject><subject>Food</subject><subject>food pathogens</subject><subject>Foodborne Diseases - prevention & control</subject><subject>gene expression</subject><subject>Gene regulation</subject><subject>genes</subject><subject>GRAS substances</subject><subject>Hemolysin Proteins - genetics</subject><subject>Hemolysin Proteins - metabolism</subject><subject>Hemolysins</subject><subject>hosts</subject><subject>Humans</subject><subject>in vivo studies</subject><subject>Infection</subject><subject>intestinal mucosa</subject><subject>Intestine</subject><subject>Lecithinase</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Listeria monocytogenes - physiology</subject><subject>Listeriosis</subject><subject>Listeriosis - prevention & control</subject><subject>Meningitis</subject><subject>microbial growth</subject><subject>Microvasculature</subject><subject>Minimum inhibitory concentration</subject><subject>Motility</subject><subject>Pathogens</subject><subject>Phospholipases - metabolism</subject><subject>Plant compounds</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants</subject><subject>polymerase chain reaction</subject><subject>Septicemia</subject><subject>thymol</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><subject>virulence factors</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS1ERYfCK4DZdUGCf-I4WaIRBaSRikRZW659PfIoiQc7mdIX4Lm50ymoS1a-sr5z7tU5hLzjrOaMtx92ddyFlPwYXU61YFzUrKkZ756RFe90X8mmZc_JCtmu4i1T5-RlKTvGmJKSvSDnQrSsa5Vekd_fBjvNlYccD-ApzvHB9TbaodAMfnFAN7HMCFg6pim5-zltYYJCDzEvA0wIBOvmlAuNE37OOb1HI099upuqDNtlsDNQ-LXPUEpME03hifbB6xU5C7gQXj--F-Tm6tPN-ku1uf78df1xU7lGNngm11Z2ELzolGpd0CrI4LX2UmvRcy-EaqToldCu9ZIzaMB2rLfKCeC-lRfk8mS7z-nnAmU2YywOBswA0lIMZ7LpBDopRPsTimGUkiGYfY6jzfcImWMLZmeetGCOLRjWGGwBtW8e1yy3I_h_yr-xI_D2BASbjN3mWMyP7-jQMJT3WCES6xMBmMYhQjbFxWNePmZws_Ep_schfwD9I6tV</recordid><startdate>20120615</startdate><enddate>20120615</enddate><creator>Upadhyay, Abhinav</creator><creator>Johny, Anup Kollanoor</creator><creator>Amalaradjou, Mary Anne Roshni</creator><creator>Ananda Baskaran, Sangeetha</creator><creator>Kim, Kwang Sik</creator><creator>Venkitanarayanan, Kumar</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20120615</creationdate><title>Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes</title><author>Upadhyay, Abhinav ; Johny, Anup Kollanoor ; Amalaradjou, Mary Anne Roshni ; Ananda Baskaran, Sangeetha ; Kim, Kwang Sik ; Venkitanarayanan, Kumar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-d17a38efd28556cf75f3fd77d377291d2254329527c6d310e4ea809a5c2e1d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenocarcinoma</topic><topic>adhesion</topic><topic>Anti-Infective Agents - pharmacology</topic><topic>Antimicrobial agents</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Proteins - physiology</topic><topic>Brain</topic><topic>Brain - cytology</topic><topic>Caco-2 Cells</topic><topic>Carvacrol</topic><topic>Cell Culture Techniques</topic><topic>Colon</topic><topic>death</topic><topic>disease control</topic><topic>Down-Regulation</topic><topic>Endothelial Cells</topic><topic>Epithelium</topic><topic>Food</topic><topic>food pathogens</topic><topic>Foodborne Diseases - prevention & control</topic><topic>gene expression</topic><topic>Gene regulation</topic><topic>genes</topic><topic>GRAS substances</topic><topic>Hemolysin Proteins - genetics</topic><topic>Hemolysin Proteins - metabolism</topic><topic>Hemolysins</topic><topic>hosts</topic><topic>Humans</topic><topic>in vivo studies</topic><topic>Infection</topic><topic>intestinal mucosa</topic><topic>Intestine</topic><topic>Lecithinase</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Listeria monocytogenes - physiology</topic><topic>Listeriosis</topic><topic>Listeriosis - prevention & control</topic><topic>Meningitis</topic><topic>microbial growth</topic><topic>Microvasculature</topic><topic>Minimum inhibitory concentration</topic><topic>Motility</topic><topic>Pathogens</topic><topic>Phospholipases - metabolism</topic><topic>Plant compounds</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants</topic><topic>polymerase chain reaction</topic><topic>Septicemia</topic><topic>thymol</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><topic>virulence factors</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Upadhyay, Abhinav</creatorcontrib><creatorcontrib>Johny, Anup Kollanoor</creatorcontrib><creatorcontrib>Amalaradjou, Mary Anne Roshni</creatorcontrib><creatorcontrib>Ananda Baskaran, Sangeetha</creatorcontrib><creatorcontrib>Kim, Kwang Sik</creatorcontrib><creatorcontrib>Venkitanarayanan, Kumar</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Upadhyay, Abhinav</au><au>Johny, Anup Kollanoor</au><au>Amalaradjou, Mary Anne Roshni</au><au>Ananda Baskaran, Sangeetha</au><au>Kim, Kwang Sik</au><au>Venkitanarayanan, Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2012-06-15</date><risdate>2012</risdate><volume>157</volume><issue>1</issue><spage>88</spage><epage>94</epage><pages>88-94</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><abstract>Listeria monocytogenes (LM) is a major foodborne pathogen causing septicemia, meningitis and death in humans. LM infection is preceded by its attachment to and invasion of human intestinal epithelium followed by systemic spread. The major virulence factors in LM include motility, hemolysin and lecithinase production. Reducing LM attachment to and invasion of host tissue and production of virulence factors could potentially control listeriosis in humans. This study investigated the efficacy of sub-inhibitory concentrations (SICs, concentrations not inhibiting bacterial growth) of three, generally regarded as safe (GRAS)-status, plant-derived antimicrobial compounds in reducing LM attachment to and invasion of human colon adenocarcinoma (Caco-2) and human brain microvascular endothelial cells (HBMEC). Additionally, the effect of these compounds on the aforementioned LM virulence factors was studied. The compounds and their respective SICs used relative to their MICs were trans-cinnamaldehyde (TC 0.50mM, 0.75mM with the MIC of 0.90mM), carvacrol (CR 0.50mM, 0.65mM with the MIC of 0.75mM), and thymol (TY 0.33mM, 0.50mM with the MIC of 0.60mM). All three-plant antimicrobials reduced LM adhesion to and invasion of Caco-2 and HBMEC (p<0.05). The compounds also decreased LM motility, hemolysin production and lecithinase activity (p<0.05). Real-time PCR data revealed that TC, CR, and TY down-regulated the expression of LM virulence genes by >3.0 folds compared to controls (p<0.05). Results suggest that TC, CR, and TY could potentially be used to control LM infection; however, in vivo studies are necessary to validate these results.
► SICs of Trans-cinnamaldehyde (TC), Carvacrol (CR), and Thymol (TY) reduced motility, hemolysin and lecithinase production. ► SICs of TC, CR, and TY completely shut down LM invasion on HBMEC and reduced adhesion and invasion on Caco-2 cells significantly. ► SICs of TC, CR, and TY down regulated expression of major virulence genes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22608657</pmid><doi>10.1016/j.ijfoodmicro.2012.04.018</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2012-06, Vol.157 (1), p.88-94 |
| issn | 0168-1605 1879-3460 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1034822915 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenocarcinoma adhesion Anti-Infective Agents - pharmacology Antimicrobial agents Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Proteins - physiology Brain Brain - cytology Caco-2 Cells Carvacrol Cell Culture Techniques Colon death disease control Down-Regulation Endothelial Cells Epithelium Food food pathogens Foodborne Diseases - prevention & control gene expression Gene regulation genes GRAS substances Hemolysin Proteins - genetics Hemolysin Proteins - metabolism Hemolysins hosts Humans in vivo studies Infection intestinal mucosa Intestine Lecithinase Listeria monocytogenes Listeria monocytogenes - genetics Listeria monocytogenes - pathogenicity Listeria monocytogenes - physiology Listeriosis Listeriosis - prevention & control Meningitis microbial growth Microvasculature Minimum inhibitory concentration Motility Pathogens Phospholipases - metabolism Plant compounds Plant Extracts - pharmacology Plants polymerase chain reaction Septicemia thymol Virulence Virulence - genetics virulence factors Virulence Factors - genetics Virulence Factors - metabolism |
| title | Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T08%3A20%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plant-derived%20antimicrobials%20reduce%20Listeria%20monocytogenes%20virulence%20factors%20in%20vitro,%20and%20down-regulate%20expression%20of%20virulence%20genes&rft.jtitle=International%20journal%20of%20food%20microbiology&rft.au=Upadhyay,%20Abhinav&rft.date=2012-06-15&rft.volume=157&rft.issue=1&rft.spage=88&rft.epage=94&rft.pages=88-94&rft.issn=0168-1605&rft.eissn=1879-3460&rft_id=info:doi/10.1016/j.ijfoodmicro.2012.04.018&rft_dat=%3Cproquest_cross%3E1034822915%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1034822915&rft_id=info:pmid/22608657&rft_els_id=S016816051200222X&rfr_iscdi=true |