Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes
Abstract：In the present study, we investigated the histological, immunohistochemical and ultrastructural characteristics of cytoplasmic blood plasma inclusions that spontaneously occurred in a rat liver. Histologically, a number of cytoplasmic inclusions were observed in the liver of an 8-week-old f...
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| Veröffentlicht in: | Journal of Toxicologic Pathology 2011, Vol.24 (4), p.245-249 |
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| container_title | Journal of Toxicologic Pathology |
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| creator | Tochitani, Tomoaki Toyosawa, Kaoru Matsumoto, Izumi Kouchi, Mami Michimae, Yoshiko Koujitani, Takatoshi Funabashi, Hitoshi Seki, Takaki |
| description | Abstract：In the present study, we investigated the histological, immunohistochemical and ultrastructural characteristics of cytoplasmic blood plasma inclusions that spontaneously occurred in a rat liver. Histologically, a number of cytoplasmic inclusions were observed in the liver of an 8-week-old female SD rat. These inclusions were strongly positive for PAS staining and resistant to diastase digestion. Immunohistochemical analyses revealed that these inclusions were positive for albumin and IgG；however, most of them were negative for LAMP-1 and LAMP-2. Ultrastructurally, the inclusions were surrounded by limiting membranes and composed of moderately electron dense, homogenous materials. These characteristics described here represent valuable information for pathological examination in toxicity studies. (DOI：10.1293/tox.24.245；J Toxicol Pathol 2011；24：245－249) Blood plasma inclusions, resulting from accumulation of blood plasma in cells, are known to occur spontaneously in the canine liver1. |
| format | Article |
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Histologically, a number of cytoplasmic inclusions were observed in the liver of an 8-week-old female SD rat. These inclusions were strongly positive for PAS staining and resistant to diastase digestion. Immunohistochemical analyses revealed that these inclusions were positive for albumin and IgG；however, most of them were negative for LAMP-1 and LAMP-2. Ultrastructurally, the inclusions were surrounded by limiting membranes and composed of moderately electron dense, homogenous materials. These characteristics described here represent valuable information for pathological examination in toxicity studies. (DOI：10.1293/tox.24.245；J Toxicol Pathol 2011；24：245－249) Blood plasma inclusions, resulting from accumulation of blood plasma in cells, are known to occur spontaneously in the canine liver1.</description><identifier>ISSN: 0914-9198</identifier><identifier>EISSN: 1881-915X</identifier><language>jpn</language><publisher>The Japanese Society of Toxicologic Pathology</publisher><subject>Albumin ; Blood ; Digestion ; Hepatocytes ; Immunoglobulin G ; Inclusion bodies ; LAMP-1 protein ; LAMP-2 protein ; Liver ; Toxicity</subject><ispartof>Journal of Toxicologic Pathology, 2011, Vol.24 (4), p.245-249</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids></links><search><creatorcontrib>Tochitani, Tomoaki</creatorcontrib><creatorcontrib>Toyosawa, Kaoru</creatorcontrib><creatorcontrib>Matsumoto, Izumi</creatorcontrib><creatorcontrib>Kouchi, Mami</creatorcontrib><creatorcontrib>Michimae, Yoshiko</creatorcontrib><creatorcontrib>Koujitani, Takatoshi</creatorcontrib><creatorcontrib>Funabashi, Hitoshi</creatorcontrib><creatorcontrib>Seki, Takaki</creatorcontrib><creatorcontrib>Dainippon Sumitomo Pharma Co</creatorcontrib><creatorcontrib>Safety Research Laboratories</creatorcontrib><creatorcontrib>Ltd</creatorcontrib><title>Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes</title><title>Journal of Toxicologic Pathology</title><description>Abstract：In the present study, we investigated the histological, immunohistochemical and ultrastructural characteristics of cytoplasmic blood plasma inclusions that spontaneously occurred in a rat liver. Histologically, a number of cytoplasmic inclusions were observed in the liver of an 8-week-old female SD rat. These inclusions were strongly positive for PAS staining and resistant to diastase digestion. Immunohistochemical analyses revealed that these inclusions were positive for albumin and IgG；however, most of them were negative for LAMP-1 and LAMP-2. Ultrastructurally, the inclusions were surrounded by limiting membranes and composed of moderately electron dense, homogenous materials. These characteristics described here represent valuable information for pathological examination in toxicity studies. (DOI：10.1293/tox.24.245；J Toxicol Pathol 2011；24：245－249) Blood plasma inclusions, resulting from accumulation of blood plasma in cells, are known to occur spontaneously in the canine liver1.</description><subject>Albumin</subject><subject>Blood</subject><subject>Digestion</subject><subject>Hepatocytes</subject><subject>Immunoglobulin G</subject><subject>Inclusion bodies</subject><subject>LAMP-1 protein</subject><subject>LAMP-2 protein</subject><subject>Liver</subject><subject>Toxicity</subject><issn>0914-9198</issn><issn>1881-915X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNotkE9LAzEQxYMoWKvfIUcvC_m7yR5r0bZQUMSCt5DdZOmWbLJuErDf3lS9zJsZfjzmzRVYYClx1WD-eQ0WqMGs9I28BXcxnhAiAnG6AKfdOGYfjkNMoTvacei0g9obeHBp1jHNuUt5LruV1-4cbYShh-tzCpPTsdDwyYVg4Ntl0nDnO5fjEPwv9q4T3NpJF-dzsvEe3PTaRfvwr0tweHn-WG-r_etmt17tqxHXklayxjUTmrQtwsIgoik3NTGCdgYh0xtc09rKlpIW8Z7zlukWSYopNQ1iggi6BI9_vtMcvrKNSY1D7Kxz2tuQo8KIMolFTWhBN3_oaM0lefBu8FadQp5L3KiMoSl8T1oRhLEqT2OIFWlU6filNOUaJqSgP0Vubfs</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Tochitani, Tomoaki</creator><creator>Toyosawa, Kaoru</creator><creator>Matsumoto, Izumi</creator><creator>Kouchi, Mami</creator><creator>Michimae, Yoshiko</creator><creator>Koujitani, Takatoshi</creator><creator>Funabashi, Hitoshi</creator><creator>Seki, Takaki</creator><general>The Japanese Society of Toxicologic Pathology</general><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>2011</creationdate><title>Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes</title><author>Tochitani, Tomoaki ; Toyosawa, Kaoru ; Matsumoto, Izumi ; Kouchi, Mami ; Michimae, Yoshiko ; Koujitani, Takatoshi ; Funabashi, Hitoshi ; Seki, Takaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-m1683-861647a2bb017d02a35d62d73cd00dfd1636e8b32b05f55b4ab083133d9047273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>jpn</language><creationdate>2011</creationdate><topic>Albumin</topic><topic>Blood</topic><topic>Digestion</topic><topic>Hepatocytes</topic><topic>Immunoglobulin G</topic><topic>Inclusion bodies</topic><topic>LAMP-1 protein</topic><topic>LAMP-2 protein</topic><topic>Liver</topic><topic>Toxicity</topic><toplevel>online_resources</toplevel><creatorcontrib>Tochitani, Tomoaki</creatorcontrib><creatorcontrib>Toyosawa, Kaoru</creatorcontrib><creatorcontrib>Matsumoto, Izumi</creatorcontrib><creatorcontrib>Kouchi, Mami</creatorcontrib><creatorcontrib>Michimae, Yoshiko</creatorcontrib><creatorcontrib>Koujitani, Takatoshi</creatorcontrib><creatorcontrib>Funabashi, Hitoshi</creatorcontrib><creatorcontrib>Seki, Takaki</creatorcontrib><creatorcontrib>Dainippon Sumitomo Pharma Co</creatorcontrib><creatorcontrib>Safety Research Laboratories</creatorcontrib><creatorcontrib>Ltd</creatorcontrib><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of Toxicologic Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tochitani, Tomoaki</au><au>Toyosawa, Kaoru</au><au>Matsumoto, Izumi</au><au>Kouchi, Mami</au><au>Michimae, Yoshiko</au><au>Koujitani, Takatoshi</au><au>Funabashi, Hitoshi</au><au>Seki, Takaki</au><aucorp>Dainippon Sumitomo Pharma Co</aucorp><aucorp>Safety Research Laboratories</aucorp><aucorp>Ltd</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes</atitle><jtitle>Journal of Toxicologic Pathology</jtitle><date>2011</date><risdate>2011</risdate><volume>24</volume><issue>4</issue><spage>245</spage><epage>249</epage><pages>245-249</pages><issn>0914-9198</issn><eissn>1881-915X</eissn><abstract>Abstract：In the present study, we investigated the histological, immunohistochemical and ultrastructural characteristics of cytoplasmic blood plasma inclusions that spontaneously occurred in a rat liver. Histologically, a number of cytoplasmic inclusions were observed in the liver of an 8-week-old female SD rat. These inclusions were strongly positive for PAS staining and resistant to diastase digestion. Immunohistochemical analyses revealed that these inclusions were positive for albumin and IgG；however, most of them were negative for LAMP-1 and LAMP-2. Ultrastructurally, the inclusions were surrounded by limiting membranes and composed of moderately electron dense, homogenous materials. These characteristics described here represent valuable information for pathological examination in toxicity studies. (DOI：10.1293/tox.24.245；J Toxicol Pathol 2011；24：245－249) Blood plasma inclusions, resulting from accumulation of blood plasma in cells, are known to occur spontaneously in the canine liver1.</abstract><pub>The Japanese Society of Toxicologic Pathology</pub><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0914-9198 |
| ispartof | Journal of Toxicologic Pathology, 2011, Vol.24 (4), p.245-249 |
| issn | 0914-9198 1881-915X |
| language | jpn |
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| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Albumin Blood Digestion Hepatocytes Immunoglobulin G Inclusion bodies LAMP-1 protein LAMP-2 protein Liver Toxicity |
| title | Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes |
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