Improved Method for Isolation of Lycopsamine from Roots of Comfrey (Symphytum officinale)
An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey (Symphytum officinale L.) roots was developed, introducing very fast, selective and ion residue-free reduction of N-oxides followed by ion-exchange chromatography giving a non-aqueous solution of alkaloids,...
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| Veröffentlicht in: | Natural product communications 2012-07, Vol.7 (7), p.861-862 |
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| container_title | Natural product communications |
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| creator | Janeš, Damjan Kalamar, Boštjan Kreft, Samo |
| description | An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey (Symphytum officinale L.) roots was developed, introducing very fast, selective and ion residue-free reduction of N-oxides followed by ion-exchange chromatography giving a non-aqueous solution of alkaloids, from which solvents can be easily removed. With this procedure the use of large volumes of organic solvents, very slow reduction of N-oxides and input of additional impurities was avoided. Lycopsamine, which proved to be the major alkaloid, was additionally purified by preparative layer chromatography (PLC) and high performance liquid chromatography (HPLC). The identity of the alkaloid was confirmed by 1H NMR spectroscopy and mass spectrometry. |
| doi_str_mv | 10.1177/1934578X1200700713 |
| format | Article |
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With this procedure the use of large volumes of organic solvents, very slow reduction of N-oxides and input of additional impurities was avoided. Lycopsamine, which proved to be the major alkaloid, was additionally purified by preparative layer chromatography (PLC) and high performance liquid chromatography (HPLC). The identity of the alkaloid was confirmed by 1H NMR spectroscopy and mass spectrometry.</description><identifier>ISSN: 1934-578X</identifier><identifier>EISSN: 1555-9475</identifier><identifier>DOI: 10.1177/1934578X1200700713</identifier><identifier>PMID: 22908565</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Alkaloids - chemistry ; Alkaloids - isolation & purification ; Chromatography, High Pressure Liquid ; Comfrey - chemistry ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Plant Roots - chemistry</subject><ispartof>Natural product communications, 2012-07, Vol.7 (7), p.861-862</ispartof><rights>2012 SAGE Publications Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-5fd62b984055ee206e5edc027a20bf7decacd6fe41ae5391da93edddef7e8b6d3</citedby><cites>FETCH-LOGICAL-c343t-5fd62b984055ee206e5edc027a20bf7decacd6fe41ae5391da93edddef7e8b6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1934578X1200700713$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1934578X1200700713$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21946,27832,27903,27904,44924,45312</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/1934578X1200700713?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22908565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Janeš, Damjan</creatorcontrib><creatorcontrib>Kalamar, Boštjan</creatorcontrib><creatorcontrib>Kreft, Samo</creatorcontrib><title>Improved Method for Isolation of Lycopsamine from Roots of Comfrey (Symphytum officinale)</title><title>Natural product communications</title><addtitle>Nat Prod Commun</addtitle><description>An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey (Symphytum officinale L.) roots was developed, introducing very fast, selective and ion residue-free reduction of N-oxides followed by ion-exchange chromatography giving a non-aqueous solution of alkaloids, from which solvents can be easily removed. With this procedure the use of large volumes of organic solvents, very slow reduction of N-oxides and input of additional impurities was avoided. Lycopsamine, which proved to be the major alkaloid, was additionally purified by preparative layer chromatography (PLC) and high performance liquid chromatography (HPLC). The identity of the alkaloid was confirmed by 1H NMR spectroscopy and mass spectrometry.</description><subject>Alkaloids - chemistry</subject><subject>Alkaloids - isolation & purification</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Comfrey - chemistry</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mass Spectrometry</subject><subject>Plant Roots - chemistry</subject><issn>1934-578X</issn><issn>1555-9475</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAUhoMobsz9AS-kl_OiLmmapr2U4cdgIvgBelXS5MR1NM1MWqH_3oxNbwQPgXNInvcl50XonOArQjifk4KmjOdvJMGYh0PoERoTxlhcpJwdhzkA8Y4Yoan3Gxwqz1OcFqdolCQFzlnGxuh9abbOfoGKHqBbWxVp66Klt43oattGVkerQdqtF6ZuIdLOmujJ2s7vXhbWaAdDNHsezHY9dL0Jt7qWdSsauDxDJ1o0HqaHPkGvtzcvi_t49Xi3XFyvYklT2sVMqyypivAzxgASnAEDJXHCRYIrzRVIIVWmISUCGC2IEgUFpRRoDnmVKTpBs71v2OOzB9-VpvYSmka0YHtfEkzTHGd5lgY02aPSWe8d6HLraiPcEKByl2r5N9Ugujj495UB9Sv5yTAA8z3gxQeUG9u7sL__z_Ibi6OBNA</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Janeš, Damjan</creator><creator>Kalamar, Boštjan</creator><creator>Kreft, Samo</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201207</creationdate><title>Improved Method for Isolation of Lycopsamine from Roots of Comfrey (Symphytum officinale)</title><author>Janeš, Damjan ; Kalamar, Boštjan ; Kreft, Samo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-5fd62b984055ee206e5edc027a20bf7decacd6fe41ae5391da93edddef7e8b6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alkaloids - chemistry</topic><topic>Alkaloids - isolation & purification</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Comfrey - chemistry</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mass Spectrometry</topic><topic>Plant Roots - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Janeš, Damjan</creatorcontrib><creatorcontrib>Kalamar, Boštjan</creatorcontrib><creatorcontrib>Kreft, Samo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Natural product communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Janeš, Damjan</au><au>Kalamar, Boštjan</au><au>Kreft, Samo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved Method for Isolation of Lycopsamine from Roots of Comfrey (Symphytum officinale)</atitle><jtitle>Natural product communications</jtitle><addtitle>Nat Prod Commun</addtitle><date>2012-07</date><risdate>2012</risdate><volume>7</volume><issue>7</issue><spage>861</spage><epage>862</epage><pages>861-862</pages><issn>1934-578X</issn><eissn>1555-9475</eissn><abstract>An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey (Symphytum officinale L.) roots was developed, introducing very fast, selective and ion residue-free reduction of N-oxides followed by ion-exchange chromatography giving a non-aqueous solution of alkaloids, from which solvents can be easily removed. 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| identifier | ISSN: 1934-578X |
| ispartof | Natural product communications, 2012-07, Vol.7 (7), p.861-862 |
| issn | 1934-578X 1555-9475 |
| language | eng |
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| source | Sage Journals GOLD Open Access 2024 |
| subjects | Alkaloids - chemistry Alkaloids - isolation & purification Chromatography, High Pressure Liquid Comfrey - chemistry Magnetic Resonance Spectroscopy Mass Spectrometry Plant Roots - chemistry |
| title | Improved Method for Isolation of Lycopsamine from Roots of Comfrey (Symphytum officinale) |
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