Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection
Sclerotinia sclerotiorum (Sclerotiniaceae) is a plant pathogenic fungus that causes white mold disease in vegetable crops, including the common bean (Phaseolus vulgaris). Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of diffe...
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| Veröffentlicht in: | Genetics and molecular research 2012-01, Vol.11 (3), p.2171-2181 |
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| creator | Bueno, E A Oliveira, M B Andrade, R V Lobo, Jr, M Petrofeza, S |
| description | Sclerotinia sclerotiorum (Sclerotiniaceae) is a plant pathogenic fungus that causes white mold disease in vegetable crops, including the common bean (Phaseolus vulgaris). Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of different carbon sources--pectin, glucose, and cell wall of P. vulgaris on the production of proteases in cultures of S. sclerotiorum. These proteases were also assayed in infected P. vulgaris plants. Enzyme activity was increased with all carbon sources, but the highest levels were found when pectin was added. Based on real-time quantitative reverse transcription-PCR analyses, protease induction in S. sclerotiorum was found to occur at the level of gene transcription. The finding of increased expression of acid phosphatase 1 and aspartyl protease in vivo in infected P. vulgaris plants supports the role of these enzymes in the invasion process of S. sclerotiorum. |
| doi_str_mv | 10.4238/2012.June.25.3 |
| format | Article |
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Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of different carbon sources--pectin, glucose, and cell wall of P. vulgaris on the production of proteases in cultures of S. sclerotiorum. These proteases were also assayed in infected P. vulgaris plants. Enzyme activity was increased with all carbon sources, but the highest levels were found when pectin was added. Based on real-time quantitative reverse transcription-PCR analyses, protease induction in S. sclerotiorum was found to occur at the level of gene transcription. 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Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of different carbon sources--pectin, glucose, and cell wall of P. vulgaris on the production of proteases in cultures of S. sclerotiorum. These proteases were also assayed in infected P. vulgaris plants. Enzyme activity was increased with all carbon sources, but the highest levels were found when pectin was added. Based on real-time quantitative reverse transcription-PCR analyses, protease induction in S. sclerotiorum was found to occur at the level of gene transcription. The finding of increased expression of acid phosphatase 1 and aspartyl protease in vivo in infected P. vulgaris plants supports the role of these enzymes in the invasion process of S. sclerotiorum.</abstract><cop>Brazil</cop><pmid>22782635</pmid><doi>10.4238/2012.June.25.3</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB Electronic Journals Library |
| subjects | Ascomycota - drug effects Ascomycota - enzymology Ascomycota - genetics Ascomycota - physiology Carbon - pharmacology Culture Media - chemistry Fungal Proteins - genetics Fungal Proteins - metabolism Gene Expression Regulation, Fungal - drug effects Genes, Fungal - genetics Hydrogen-Ion Concentration - drug effects Kinetics Peptide Hydrolases - genetics Peptide Hydrolases - secretion Phaseolus - drug effects Phaseolus - microbiology Plant Diseases - microbiology Protease Inhibitors - pharmacology Proteolysis - drug effects Real-Time Polymerase Chain Reaction Transcription, Genetic - drug effects |
| title | Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection |
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