Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation
In this article, we introduce a chip-capillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) or capillary gel electrophoresis (CGE) toward automating two-dimensional (2D) protein separati...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2012-08, Vol.84 (16), p.7001-7007 |
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| creator | Lu, Joann J Wang, Shili Li, Guanbin Wang, Wei Pu, Qiaosheng Liu, Shaorong |
| description | In this article, we introduce a chip-capillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) or capillary gel electrophoresis (CGE) toward automating two-dimensional (2D) protein separations. The hybrid device consists of three chips that are butted together. The middle chip can be moved between two positions to reroute the fluidic paths, which enables the performance of CIEF and injection of proteins partially resolved by CIEF to CGE capillaries for parallel CGE separations in a continuous and automated fashion. Capillaries are attached to the other two chips to facilitate CIEF and CGE separations and to extend the effective lengths of CGE columns. Specifically, we illustrate the working principle of the hybrid device, develop protocols for producing and preparing the hybrid device, and demonstrate the feasibility of using this hybrid device for automated injection of CIEF-separated sample to parallel CGE for 2D protein separations. Potentials and problems associated with the hybrid device are also discussed. |
| doi_str_mv | 10.1021/ac3017168 |
| format | Article |
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The hybrid device consists of three chips that are butted together. The middle chip can be moved between two positions to reroute the fluidic paths, which enables the performance of CIEF and injection of proteins partially resolved by CIEF to CGE capillaries for parallel CGE separations in a continuous and automated fashion. Capillaries are attached to the other two chips to facilitate CIEF and CGE separations and to extend the effective lengths of CGE columns. Specifically, we illustrate the working principle of the hybrid device, develop protocols for producing and preparing the hybrid device, and demonstrate the feasibility of using this hybrid device for automated injection of CIEF-separated sample to parallel CGE for 2D protein separations. Potentials and problems associated with the hybrid device are also discussed.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac3017168</identifier><identifier>PMID: 22830584</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Animals ; Automation ; Cattle ; Chemical compounds ; Chemical reactions ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Electrophoresis, Microchip - instrumentation ; Electrophoresis, Polyacrylamide Gel - instrumentation ; Exact sciences and technology ; Isoelectric Focusing - instrumentation ; Other chromatographic methods ; Proteins ; Proteins - isolation & purification ; Protocol</subject><ispartof>Analytical chemistry (Washington), 2012-08, Vol.84 (16), p.7001-7007</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Aug 21, 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a408t-3df4cf335ef7721b179cd4515f2193007ef9597ce450641b782c590a39381c4e3</citedby><cites>FETCH-LOGICAL-a408t-3df4cf335ef7721b179cd4515f2193007ef9597ce450641b782c590a39381c4e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac3017168$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac3017168$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26281476$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22830584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Joann J</creatorcontrib><creatorcontrib>Wang, Shili</creatorcontrib><creatorcontrib>Li, Guanbin</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Pu, Qiaosheng</creatorcontrib><creatorcontrib>Liu, Shaorong</creatorcontrib><title>Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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Potentials and problems associated with the hybrid device are also discussed.</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Automation</subject><subject>Cattle</subject><subject>Chemical compounds</subject><subject>Chemical reactions</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Electrophoresis, Microchip - instrumentation</subject><subject>Electrophoresis, Polyacrylamide Gel - instrumentation</subject><subject>Exact sciences and technology</subject><subject>Isoelectric Focusing - instrumentation</subject><subject>Other chromatographic methods</subject><subject>Proteins</subject><subject>Proteins - isolation & purification</subject><subject>Protocol</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0ctq3DAUBmARWpJpmkVfoAhKoV241cWy5GWYXCHQQCZrI8tHjYJsuZKdMg_Xd6s6M82ErITlj_Mf9CP0gZJvlDD6XRtOqKSVOkALKhgpKqXYG7QghPCCSUKO0LuUHgmhlNDqEB0xpjgRqlygP8sHNxZLPTrvdVzjq3UbXYfP4MkZwDZEfDpPodcTdHgV9ZAsRBwsvtP96AHfRkgw6rj5367xftB1CuDBTNEZfBHMnNzwE08B32bsPfgX9DJ_nW9sGB9CnujSJnn1OxRnrochuTBon8PCBG7Ad9vEfPkevbXaJzjZncfo_uJ8tbwqbn5cXi9PbwpdEjUVvLOlsZwLsFIy2lJZm64UVFhGa06IBFuLWhooBalK2krFjKiJ5jVX1JTAj9GX7dwxhl8zpKnpXTKQ1x8gzKmhhJeVUFyxTD-9oo9hjnn9jRJMCKVkVl-3ysSQUgTbjNH1-TEyav512jx3mu3H3cS57aF7lv9LzODzDuhktLe5JuPS3lVM0VJWe6dNernV68C_M-y2Mw</recordid><startdate>20120821</startdate><enddate>20120821</enddate><creator>Lu, Joann J</creator><creator>Wang, Shili</creator><creator>Li, Guanbin</creator><creator>Wang, Wei</creator><creator>Pu, Qiaosheng</creator><creator>Liu, Shaorong</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120821</creationdate><title>Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation</title><author>Lu, Joann J ; Wang, Shili ; Li, Guanbin ; Wang, Wei ; Pu, Qiaosheng ; Liu, Shaorong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a408t-3df4cf335ef7721b179cd4515f2193007ef9597ce450641b782c590a39381c4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Automation</topic><topic>Cattle</topic><topic>Chemical compounds</topic><topic>Chemical reactions</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Electrophoresis, Microchip - instrumentation</topic><topic>Electrophoresis, Polyacrylamide Gel - instrumentation</topic><topic>Exact sciences and technology</topic><topic>Isoelectric Focusing - instrumentation</topic><topic>Other chromatographic methods</topic><topic>Proteins</topic><topic>Proteins - isolation & purification</topic><topic>Protocol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Joann J</creatorcontrib><creatorcontrib>Wang, Shili</creatorcontrib><creatorcontrib>Li, Guanbin</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Pu, Qiaosheng</creatorcontrib><creatorcontrib>Liu, Shaorong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Joann J</au><au>Wang, Shili</au><au>Li, Guanbin</au><au>Wang, Wei</au><au>Pu, Qiaosheng</au><au>Liu, Shaorong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2012-08-21</date><risdate>2012</risdate><volume>84</volume><issue>16</issue><spage>7001</spage><epage>7007</epage><pages>7001-7007</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>In this article, we introduce a chip-capillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) or capillary gel electrophoresis (CGE) toward automating two-dimensional (2D) protein separations. The hybrid device consists of three chips that are butted together. The middle chip can be moved between two positions to reroute the fluidic paths, which enables the performance of CIEF and injection of proteins partially resolved by CIEF to CGE capillaries for parallel CGE separations in a continuous and automated fashion. 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| source | MEDLINE; American Chemical Society Web Editions |
| subjects | Analytical chemistry Animals Automation Cattle Chemical compounds Chemical reactions Chemistry Chromatographic methods and physical methods associated with chromatography Electrophoresis, Microchip - instrumentation Electrophoresis, Polyacrylamide Gel - instrumentation Exact sciences and technology Isoelectric Focusing - instrumentation Other chromatographic methods Proteins Proteins - isolation & purification Protocol |
| title | Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation |
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