The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium
Abstract This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblasti...
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| Veröffentlicht in: | International journal of oral and maxillofacial surgery 2012-09, Vol.41 (9), p.1097-1101 |
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| container_title | International journal of oral and maxillofacial surgery |
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| creator | Teixeira, L.N Crippa, G.E Lefebvre, L.-P De Oliveira, P.T Rosa, A.L Beloti, M.M |
| description | Abstract This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 ( p = 0.0010), day 7 ( p = 0.0005) and day 10 ( p = 0.0090) in the following way: Ti 62 < Ti 130 < Ti 312. Gene expression of bone markers evaluated at 14 days was affected, RUNX2 ( p = 0.0153), ALP ( p = 0.0153), BSP ( p = 0.0156), COL ( p = 0.0156), and OPN ( p = 0.0156) by pore size as follows: Ti 312 < Ti 130 < Ti 62. Based on these results, the authors suggest that porous Ti surfaces with pore sizes near 62 μm, compared with those of 312 μm and 130 μm, yield the highest expression of osteoblast phenotype as indicated by the lower cell proliferation rate and higher gene expression of bone markers. |
| doi_str_mv | 10.1016/j.ijom.2012.02.020 |
| format | Article |
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Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 ( p = 0.0010), day 7 ( p = 0.0005) and day 10 ( p = 0.0090) in the following way: Ti 62 < Ti 130 < Ti 312. Gene expression of bone markers evaluated at 14 days was affected, RUNX2 ( p = 0.0153), ALP ( p = 0.0153), BSP ( p = 0.0156), COL ( p = 0.0156), and OPN ( p = 0.0156) by pore size as follows: Ti 312 < Ti 130 < Ti 62. Based on these results, the authors suggest that porous Ti surfaces with pore sizes near 62 μm, compared with those of 312 μm and 130 μm, yield the highest expression of osteoblast phenotype as indicated by the lower cell proliferation rate and higher gene expression of bone markers.</description><identifier>ISSN: 0901-5027</identifier><identifier>EISSN: 1399-0020</identifier><identifier>DOI: 10.1016/j.ijom.2012.02.020</identifier><identifier>PMID: 22487807</identifier><identifier>CODEN: IJOSE9</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adult ; Alveolar Process - cytology ; Biological and medical sciences ; cell culture ; Cell Culture Techniques ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; dental implant ; Dentistry ; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics ; Humans ; Male ; Maxillofacial surgery. Dental surgery. Orthodontics ; Medical sciences ; osteoblast ; Osteoblasts - cytology ; Otorhinolaryngology. Stomatology ; pore size ; Porosity ; porous titanium ; Surface Properties ; Surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Titanium - chemistry ; Young Adult</subject><ispartof>International journal of oral and maxillofacial surgery, 2012-09, Vol.41 (9), p.1097-1101</ispartof><rights>International Association of Oral and Maxillofacial Surgeons</rights><rights>2012 International Association of Oral and Maxillofacial Surgeons</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-1fbb7e168c1a07a6b81bc2c2056208cd87e54a64c267a185e634be043ef4ce883</citedby><cites>FETCH-LOGICAL-c485t-1fbb7e168c1a07a6b81bc2c2056208cd87e54a64c267a185e634be043ef4ce883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0901502712000884$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26270316$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22487807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teixeira, L.N</creatorcontrib><creatorcontrib>Crippa, G.E</creatorcontrib><creatorcontrib>Lefebvre, L.-P</creatorcontrib><creatorcontrib>De Oliveira, P.T</creatorcontrib><creatorcontrib>Rosa, A.L</creatorcontrib><creatorcontrib>Beloti, M.M</creatorcontrib><title>The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium</title><title>International journal of oral and maxillofacial surgery</title><addtitle>Int J Oral Maxillofac Surg</addtitle><description>Abstract This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 ( p = 0.0010), day 7 ( p = 0.0005) and day 10 ( p = 0.0090) in the following way: Ti 62 < Ti 130 < Ti 312. Gene expression of bone markers evaluated at 14 days was affected, RUNX2 ( p = 0.0153), ALP ( p = 0.0153), BSP ( p = 0.0156), COL ( p = 0.0156), and OPN ( p = 0.0156) by pore size as follows: Ti 312 < Ti 130 < Ti 62. Based on these results, the authors suggest that porous Ti surfaces with pore sizes near 62 μm, compared with those of 312 μm and 130 μm, yield the highest expression of osteoblast phenotype as indicated by the lower cell proliferation rate and higher gene expression of bone markers.</description><subject>Adult</subject><subject>Alveolar Process - cytology</subject><subject>Biological and medical sciences</subject><subject>cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>dental implant</subject><subject>Dentistry</subject><subject>Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics</subject><subject>Humans</subject><subject>Male</subject><subject>Maxillofacial surgery. Dental surgery. Orthodontics</subject><subject>Medical sciences</subject><subject>osteoblast</subject><subject>Osteoblasts - cytology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>pore size</subject><subject>Porosity</subject><subject>porous titanium</subject><subject>Surface Properties</subject><subject>Surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Titanium - chemistry</subject><subject>Young Adult</subject><issn>0901-5027</issn><issn>1399-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk2r1DAUhoMo3vHqH3Ah2QhuOp58tE1BBLn4BRdceF2HNHPqTW2TmqTq-OtNmVHBhXAgOeR53xxeDiGPGewZsOb5uHdjmPccGN_DVnCH7JjougrK_S7ZQQesqoG3F-RBSiMAdEK198kF51K1Ctod6W9ukTo_TCt6izQMdAkRaXI_S-NpSBlDP5mU6XKLPuTjghR_LBFTcuXdeWrXKa-lp59j-O43UXEIa6LZZePdOj8k9wYzJXx0Pi_Jpzevb67eVdcf3r6_enVdWanqXLGh71tkjbLMQGuaXrHecsuhbjgoe1At1tI00vKmNUzV2AjZI0iBg7SolLgkz06-SwxfV0xZzy5ZnCbjscyjGQjJOiU4Kyg_oTaGlCIOeoluNvFYIL1lq0e9Zau3bDVsBUX05Oy_9jMe_kh-h1mAp2fAJGumIRpvXfrLNbwFwZrCvThxWNL45jDqZN0W_8FFtFkfgvv_HC__kdvJeVd-_IJHTGNYoy85a6ZTEeiP2xZsS8B4WQClpPgFVUatkA</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Teixeira, L.N</creator><creator>Crippa, G.E</creator><creator>Lefebvre, L.-P</creator><creator>De Oliveira, P.T</creator><creator>Rosa, A.L</creator><creator>Beloti, M.M</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120901</creationdate><title>The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium</title><author>Teixeira, L.N ; Crippa, G.E ; Lefebvre, L.-P ; De Oliveira, P.T ; Rosa, A.L ; Beloti, M.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-1fbb7e168c1a07a6b81bc2c2056208cd87e54a64c267a185e634be043ef4ce883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Alveolar Process - cytology</topic><topic>Biological and medical sciences</topic><topic>cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>dental implant</topic><topic>Dentistry</topic><topic>Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics</topic><topic>Humans</topic><topic>Male</topic><topic>Maxillofacial surgery. Dental surgery. Orthodontics</topic><topic>Medical sciences</topic><topic>osteoblast</topic><topic>Osteoblasts - cytology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>pore size</topic><topic>Porosity</topic><topic>porous titanium</topic><topic>Surface Properties</topic><topic>Surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Titanium - chemistry</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teixeira, L.N</creatorcontrib><creatorcontrib>Crippa, G.E</creatorcontrib><creatorcontrib>Lefebvre, L.-P</creatorcontrib><creatorcontrib>De Oliveira, P.T</creatorcontrib><creatorcontrib>Rosa, A.L</creatorcontrib><creatorcontrib>Beloti, M.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of oral and maxillofacial surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teixeira, L.N</au><au>Crippa, G.E</au><au>Lefebvre, L.-P</au><au>De Oliveira, P.T</au><au>Rosa, A.L</au><au>Beloti, M.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium</atitle><jtitle>International journal of oral and maxillofacial surgery</jtitle><addtitle>Int J Oral Maxillofac Surg</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>41</volume><issue>9</issue><spage>1097</spage><epage>1101</epage><pages>1097-1101</pages><issn>0901-5027</issn><eissn>1399-0020</eissn><coden>IJOSE9</coden><abstract>Abstract This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 ( p = 0.0010), day 7 ( p = 0.0005) and day 10 ( p = 0.0090) in the following way: Ti 62 < Ti 130 < Ti 312. Gene expression of bone markers evaluated at 14 days was affected, RUNX2 ( p = 0.0153), ALP ( p = 0.0153), BSP ( p = 0.0156), COL ( p = 0.0156), and OPN ( p = 0.0156) by pore size as follows: Ti 312 < Ti 130 < Ti 62. 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| subjects | Adult Alveolar Process - cytology Biological and medical sciences cell culture Cell Culture Techniques Cell Differentiation Cell Proliferation Cells, Cultured dental implant Dentistry Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics Humans Male Maxillofacial surgery. Dental surgery. Orthodontics Medical sciences osteoblast Osteoblasts - cytology Otorhinolaryngology. Stomatology pore size Porosity porous titanium Surface Properties Surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Titanium - chemistry Young Adult |
| title | The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium |
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