Antibody–antigen pair probed by combinatorial approach and rational design: Bringing together structural insights, directed evolution, and novel functionality
► Antibodies are extraordinary template for combinatorial and rational improvements. ► Reactibodies are novel catalytic scavengers with predefined specificity. ► Catalytic antibodies towards distinct pathogens – elaboration of catalytic vaccines. ► 2D screening – antibody versus antigen in solving o...
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| Veröffentlicht in: | FEBS letters 2012-08, Vol.586 (18), p.2966-2973 |
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| container_title | FEBS letters |
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| creator | Belogurov, Alexey Smirnov, Ivan Ponomarenko, Natalya Gabibov, Alexander |
| description | ► Antibodies are extraordinary template for combinatorial and rational improvements. ► Reactibodies are novel catalytic scavengers with predefined specificity. ► Catalytic antibodies towards distinct pathogens – elaboration of catalytic vaccines. ► 2D screening – antibody versus antigen in solving of the “Chicken and Egg” problem.
The unique hypervariability of the immunoglobulin (Ig) superfamily provides a means to create both binding and catalytic antibodies with almost any desired specificity and activity. The diversity of antigens and concept of adaptive response suggest that it is possible to find an antigen pair to any raised Ig. In the current review we discuss combinatorial approaches, which makes it possible to obtain an antibody with predefined properties, followed by 3D structure-based rational design to enhance or dramatically change its characteristics. A similar strategy, but applied to the second partner of the antibody–antigen pair, may result in selection of complementary substrates to the chosen Ig. Finally, 2D screening may be performed solving the “Chicken and Egg” problem when neither antibody nor antigen is known. |
| doi_str_mv | 10.1016/j.febslet.2012.07.046 |
| format | Article |
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The unique hypervariability of the immunoglobulin (Ig) superfamily provides a means to create both binding and catalytic antibodies with almost any desired specificity and activity. The diversity of antigens and concept of adaptive response suggest that it is possible to find an antigen pair to any raised Ig. In the current review we discuss combinatorial approaches, which makes it possible to obtain an antibody with predefined properties, followed by 3D structure-based rational design to enhance or dramatically change its characteristics. A similar strategy, but applied to the second partner of the antibody–antigen pair, may result in selection of complementary substrates to the chosen Ig. Finally, 2D screening may be performed solving the “Chicken and Egg” problem when neither antibody nor antigen is known.</description><subject>Antigen-Antibody Reactions</subject><subject>Autoantibodies</subject><subject>Autoantigen</subject><subject>Catalytic vaccine</subject><subject>Combinatorial Chemistry Techniques</subject><subject>Combinatorial screening</subject><subject>Drug Design</subject><subject>Evolution, Chemical</subject><subject>Multiple sclerosis</subject><subject>Rational design</subject><subject>Reactibodies</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNqNkU1u1DAYhiMEotPCEUBesmgGO7HjhA1qq5YiVWIBSN1Z_vky41HGHmxnqtlxBy7Qs3ESHDKwBcmS_x4_tr-3KF4RvCSYNG83yx5UHCAtK0yqJeZLTJsnxYK0vC5r2rRPiwXGhJaMd_VJcRrjBud5S7rnxUlVtZRwwhfF44VLVnlz-Pn9h8zDFTi0kzagXfAKDFIHpP1WWSeTD1YOSO7yjtRrJJ1BQSbrXV41EO3KvUOXwbpVbij5FaQ1BBRTGHUaQ4asy9A6xXNkbACdsh72fhgnx_lvn_N7GFA_Oj17bTq8KJ71cojw8tifFV9vrr9c3ZZ3nz58vLq4KzXl_L4kpuHMGEJ7xnSLO1nlOnDddTVmClhPCJadVKpVmlKuGNWkN5VmrG85qL6qz4o3szd_79sIMYmtjRqGQTrwYxQE1zVlFcVtRtmM6uBjDNCLXbBbGQ4ZElM4YiOO4YgpHIG5yOHkc6-PV4xqC-bvqT9pZOB2Bh7sAIf_s4qb68vq85T0FDSpMG4Ivc-q97MKcs32FoKI2oLTMJdeGG__8dpfR0--QQ</recordid><startdate>20120831</startdate><enddate>20120831</enddate><creator>Belogurov, Alexey</creator><creator>Smirnov, Ivan</creator><creator>Ponomarenko, Natalya</creator><creator>Gabibov, Alexander</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120831</creationdate><title>Antibody–antigen pair probed by combinatorial approach and rational design: Bringing together structural insights, directed evolution, and novel functionality</title><author>Belogurov, Alexey ; Smirnov, Ivan ; Ponomarenko, Natalya ; Gabibov, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477X-1d675dd14f55c809a28737c99305be5f110a9abb8bc447b54c1fd2c55f87ebf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antigen-Antibody Reactions</topic><topic>Autoantibodies</topic><topic>Autoantigen</topic><topic>Catalytic vaccine</topic><topic>Combinatorial Chemistry Techniques</topic><topic>Combinatorial screening</topic><topic>Drug Design</topic><topic>Evolution, Chemical</topic><topic>Multiple sclerosis</topic><topic>Rational design</topic><topic>Reactibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belogurov, Alexey</creatorcontrib><creatorcontrib>Smirnov, Ivan</creatorcontrib><creatorcontrib>Ponomarenko, Natalya</creatorcontrib><creatorcontrib>Gabibov, Alexander</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belogurov, Alexey</au><au>Smirnov, Ivan</au><au>Ponomarenko, Natalya</au><au>Gabibov, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibody–antigen pair probed by combinatorial approach and rational design: Bringing together structural insights, directed evolution, and novel functionality</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2012-08-31</date><risdate>2012</risdate><volume>586</volume><issue>18</issue><spage>2966</spage><epage>2973</epage><pages>2966-2973</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>► Antibodies are extraordinary template for combinatorial and rational improvements. ► Reactibodies are novel catalytic scavengers with predefined specificity. ► Catalytic antibodies towards distinct pathogens – elaboration of catalytic vaccines. ► 2D screening – antibody versus antigen in solving of the “Chicken and Egg” problem.
The unique hypervariability of the immunoglobulin (Ig) superfamily provides a means to create both binding and catalytic antibodies with almost any desired specificity and activity. The diversity of antigens and concept of adaptive response suggest that it is possible to find an antigen pair to any raised Ig. In the current review we discuss combinatorial approaches, which makes it possible to obtain an antibody with predefined properties, followed by 3D structure-based rational design to enhance or dramatically change its characteristics. A similar strategy, but applied to the second partner of the antibody–antigen pair, may result in selection of complementary substrates to the chosen Ig. Finally, 2D screening may be performed solving the “Chicken and Egg” problem when neither antibody nor antigen is known.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>22841717</pmid><doi>10.1016/j.febslet.2012.07.046</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Free Content; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Antigen-Antibody Reactions Autoantibodies Autoantigen Catalytic vaccine Combinatorial Chemistry Techniques Combinatorial screening Drug Design Evolution, Chemical Multiple sclerosis Rational design Reactibodies |
| title | Antibody–antigen pair probed by combinatorial approach and rational design: Bringing together structural insights, directed evolution, and novel functionality |
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