A transgenic Marc-145 cell line of piggyBac transposon-derived targeting shRNA interference against porcine reproductive and respiratory syndrome virus
Porcine reproductive and respiratory syndrome (PRRS) is now considered to be one of the most important diseases in countries with intensive swine industries. The two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus (PRRSV), GP5 and M (encoded by ORF5 and ORF6...
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| Veröffentlicht in: | Veterinary research communications 2012-06, Vol.36 (2), p.99-105 |
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| creator | Zhou, Fang Liang, Shuang Chen, An-hui Singh, Chabungbam Orville Bhaskar, Roy Niu, Yan-shan Miao, Yun-gen |
| description | Porcine reproductive and respiratory syndrome (PRRS) is now considered to be one of the most important diseases in countries with intensive swine industries. The two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus (PRRSV), GP5 and M (encoded by ORF5 and ORF6 genes, respectively), are associated as disulfide-linked heterodimers (GP5/M) in the virus particle. In this study, we designed 5 of the small hairpin RNAs (shRNAs) targeting the GP5 and M gene of PRRSV respectively, and investigated their inhibition to the production of PRRSV. The highest activity displayed in shRNAs of the ORF6e sequence (nts 261-279), which the inhibition rate reached was 99.09%. The result suggests that RNAi technology might serve as a potential molecular strategy for PRRSV therapy. Furthermore, the transgenic Marc-145 cell line of
piggy
Bac transposon-derived targeting shRNA interference against PRRS virus was established. It presented stable inhibition to the replication and amplification of PRRS. The work implied that shRNAs targeting the GP5 and M gene of PRRSV may be used as potential RNA vaccines in vivo, and supplied the screening methods of transformed pig embryonic fibroblast which are prerequisite for the disease-resistant transgenic pigs to PRRS. |
| doi_str_mv | 10.1007/s11259-012-9519-9 |
| format | Article |
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piggy
Bac transposon-derived targeting shRNA interference against PRRS virus was established. It presented stable inhibition to the replication and amplification of PRRS. The work implied that shRNAs targeting the GP5 and M gene of PRRSV may be used as potential RNA vaccines in vivo, and supplied the screening methods of transformed pig embryonic fibroblast which are prerequisite for the disease-resistant transgenic pigs to PRRS.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-012-9519-9</identifier><identifier>PMID: 22297554</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Biomedical and Life Sciences ; Cell Line ; Cloning, Molecular ; Gene Expression Regulation, Viral - physiology ; Life Sciences ; Original Article ; Plasmids ; Porcine respiratory and reproductive syndrome virus ; Porcine respiratory and reproductive syndrome virus - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Interference - physiology ; RNA, Small Interfering - genetics ; Swine ; Transfection ; Veterinary Medicine/Veterinary Science ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - metabolism ; Zoology</subject><ispartof>Veterinary research communications, 2012-06, Vol.36 (2), p.99-105</ispartof><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-b54f34d86b610e15f38d293f119da861a960ac5c3ece438c84b856e29744f2663</citedby><cites>FETCH-LOGICAL-c448t-b54f34d86b610e15f38d293f119da861a960ac5c3ece438c84b856e29744f2663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-012-9519-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-012-9519-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22297554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Fang</creatorcontrib><creatorcontrib>Liang, Shuang</creatorcontrib><creatorcontrib>Chen, An-hui</creatorcontrib><creatorcontrib>Singh, Chabungbam Orville</creatorcontrib><creatorcontrib>Bhaskar, Roy</creatorcontrib><creatorcontrib>Niu, Yan-shan</creatorcontrib><creatorcontrib>Miao, Yun-gen</creatorcontrib><title>A transgenic Marc-145 cell line of piggyBac transposon-derived targeting shRNA interference against porcine reproductive and respiratory syndrome virus</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>Porcine reproductive and respiratory syndrome (PRRS) is now considered to be one of the most important diseases in countries with intensive swine industries. The two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus (PRRSV), GP5 and M (encoded by ORF5 and ORF6 genes, respectively), are associated as disulfide-linked heterodimers (GP5/M) in the virus particle. In this study, we designed 5 of the small hairpin RNAs (shRNAs) targeting the GP5 and M gene of PRRSV respectively, and investigated their inhibition to the production of PRRSV. The highest activity displayed in shRNAs of the ORF6e sequence (nts 261-279), which the inhibition rate reached was 99.09%. The result suggests that RNAi technology might serve as a potential molecular strategy for PRRSV therapy. Furthermore, the transgenic Marc-145 cell line of
piggy
Bac transposon-derived targeting shRNA interference against PRRS virus was established. It presented stable inhibition to the replication and amplification of PRRS. The work implied that shRNAs targeting the GP5 and M gene of PRRSV may be used as potential RNA vaccines in vivo, and supplied the screening methods of transformed pig embryonic fibroblast which are prerequisite for the disease-resistant transgenic pigs to PRRS.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>Gene Expression Regulation, Viral - physiology</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Plasmids</subject><subject>Porcine respiratory and reproductive syndrome virus</subject><subject>Porcine respiratory and reproductive syndrome virus - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Interference - physiology</subject><subject>RNA, Small Interfering - genetics</subject><subject>Swine</subject><subject>Transfection</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - metabolism</subject><subject>Zoology</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkcGKFDEURYMoTjv6AW4k4MZNNKkkVcmyHUZnYFQQXRfp1KsyQ3dSvqQG-kv8XdPUKCIIrgLJeTfvcgh5LvhrwXn3JgvRaMu4aJjVwjL7gGyE7iTrlGofkg0XrWadNPyMPMn5lnNuDZePyVnTNLbTWm3Ijy0t6GKeIAZPPzj0TChNPez3dB8i0DTSOUzT8a3zKzmnnCIbAMMdDLQ4nKCEONH87fPHLQ2xAI6AED1QN7kQc6FzQn_KQpgxDYsvdZS6ONSLPAd0JeGR5mMcMB2A3gVc8lPyaHT7DM_uz3Py9d3ll4srdvPp_fXF9oZ5pUxhO61GqQbT7lrBQehRmqGxchTCDs60wtmWO6-9BA9KGm_UzugWanulxqZt5Tl5tebWzb4vkEt_CPnU3kVIS-4Fl43lSir1P2iVopWRFX35F3qbFoy1SKVEZ5XsrKiUWCmPKWeEsZ8xHBweK9SfBPer4L4K7k-Ce1tnXtwnL7sDDL8nfhmtQLMCuT7FCfDPr_-V-hMJJbHF</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Zhou, Fang</creator><creator>Liang, Shuang</creator><creator>Chen, An-hui</creator><creator>Singh, Chabungbam Orville</creator><creator>Bhaskar, Roy</creator><creator>Niu, Yan-shan</creator><creator>Miao, Yun-gen</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20120601</creationdate><title>A transgenic Marc-145 cell line of piggyBac transposon-derived targeting shRNA interference against porcine reproductive and respiratory syndrome virus</title><author>Zhou, Fang ; Liang, Shuang ; Chen, An-hui ; Singh, Chabungbam Orville ; Bhaskar, Roy ; Niu, Yan-shan ; Miao, Yun-gen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-b54f34d86b610e15f38d293f119da861a960ac5c3ece438c84b856e29744f2663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>Gene Expression Regulation, Viral - physiology</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Plasmids</topic><topic>Porcine respiratory and reproductive syndrome virus</topic><topic>Porcine respiratory and reproductive syndrome virus - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA Interference - physiology</topic><topic>RNA, Small Interfering - genetics</topic><topic>Swine</topic><topic>Transfection</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - metabolism</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Fang</creatorcontrib><creatorcontrib>Liang, Shuang</creatorcontrib><creatorcontrib>Chen, An-hui</creatorcontrib><creatorcontrib>Singh, Chabungbam Orville</creatorcontrib><creatorcontrib>Bhaskar, Roy</creatorcontrib><creatorcontrib>Niu, Yan-shan</creatorcontrib><creatorcontrib>Miao, Yun-gen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Fang</au><au>Liang, Shuang</au><au>Chen, An-hui</au><au>Singh, Chabungbam Orville</au><au>Bhaskar, Roy</au><au>Niu, Yan-shan</au><au>Miao, Yun-gen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A transgenic Marc-145 cell line of piggyBac transposon-derived targeting shRNA interference against porcine reproductive and respiratory syndrome virus</atitle><jtitle>Veterinary research communications</jtitle><stitle>Vet Res Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>36</volume><issue>2</issue><spage>99</spage><epage>105</epage><pages>99-105</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>Porcine reproductive and respiratory syndrome (PRRS) is now considered to be one of the most important diseases in countries with intensive swine industries. The two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus (PRRSV), GP5 and M (encoded by ORF5 and ORF6 genes, respectively), are associated as disulfide-linked heterodimers (GP5/M) in the virus particle. In this study, we designed 5 of the small hairpin RNAs (shRNAs) targeting the GP5 and M gene of PRRSV respectively, and investigated their inhibition to the production of PRRSV. The highest activity displayed in shRNAs of the ORF6e sequence (nts 261-279), which the inhibition rate reached was 99.09%. The result suggests that RNAi technology might serve as a potential molecular strategy for PRRSV therapy. Furthermore, the transgenic Marc-145 cell line of
piggy
Bac transposon-derived targeting shRNA interference against PRRS virus was established. It presented stable inhibition to the replication and amplification of PRRS. The work implied that shRNAs targeting the GP5 and M gene of PRRSV may be used as potential RNA vaccines in vivo, and supplied the screening methods of transformed pig embryonic fibroblast which are prerequisite for the disease-resistant transgenic pigs to PRRS.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>22297554</pmid><doi>10.1007/s11259-012-9519-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Veterinary research communications, 2012-06, Vol.36 (2), p.99-105 |
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| subjects | Animals Biomedical and Life Sciences Cell Line Cloning, Molecular Gene Expression Regulation, Viral - physiology Life Sciences Original Article Plasmids Porcine respiratory and reproductive syndrome virus Porcine respiratory and reproductive syndrome virus - physiology Reverse Transcriptase Polymerase Chain Reaction RNA Interference - physiology RNA, Small Interfering - genetics Swine Transfection Veterinary Medicine/Veterinary Science Viral Envelope Proteins - genetics Viral Envelope Proteins - metabolism Zoology |
| title | A transgenic Marc-145 cell line of piggyBac transposon-derived targeting shRNA interference against porcine reproductive and respiratory syndrome virus |
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