Replication-competent gamma-retrovirus Mo-MuLV expressing green fluorescent protein as efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as primary cell receptor
The effect of sulfated polysaccharides on the efficiency of the infection of mouse transplantable fibroblast SC-1 and NIH-3T3 cell lines by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV), which carries the eGFP gene, was investigated. It was found that sulfated polysacchar...
Gespeichert in:
| Veröffentlicht in: | Molecular biology (New York) 2012-05, Vol.46 (3), p.457-466 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 466 |
|---|---|
| container_issue | 3 |
| container_start_page | 457 |
| container_title | Molecular biology (New York) |
| container_volume | 46 |
| creator | Stepanov, O. A. Prokof’eva, M. M. Stocking, K. Varlamov, V. P. Levov, A. N. Vikhoreva, G. A. Spirin, P. V. Mikhailov, S. N. Prassolov, V. S. |
| description | The effect of sulfated polysaccharides on the efficiency of the infection of mouse transplantable fibroblast SC-1 and NIH-3T3 cell lines by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV), which carries the eGFP gene, was investigated. It was found that sulfated polysaccharides have no cytostatic and cytotoxic effects on SC-1 and NIH-3T3 cells in the concentration range of 0.01–100 μg/mL and have virucidal activity against Mo-MuLV. Polysaccharides in the indicated concentrations inhibit Mo-MuLV infection that prevents the further development of viral infection. It was shown that sulfated polysaccharides are also effective inhibitors of other retroviruses, including lentiviruses, which use sulfated polysaccharides as primary cell receptors. |
| doi_str_mv | 10.1134/S0026893312030119 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1032899273</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1032899273</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-43d3df2d153ef9950657e582759abec63929fc30a5efaa1b9d3a7130727bd333</originalsourceid><addsrcrecordid>eNp1kcGKFDEQhoMoOK4-gLeAFy-tSWp6unOURVdhFkEXr00mXZnJ0p20qbTo8_liJsyCi4sQSKrq__5UUYy9lOKNlLB9-1UItes1gFQChJT6EdvInegbUNv2MdvUclPrT9kzolshZDlqw35_wWXy1mQfQ2PjvGDGkPnRzLNpEuYUf_i0Er-OzfW6_8bx55KQyIcjPybEwN20xpKxlVpSzOgDN8TROW99TeYYJ-5i4mQrUMnouA8nf_A5JqrR34-QeD6ZzMuLn3AxyQRO6-RMxmq7JD-b9ItbnKZCWVyKxXP2xJmJ8MXdfcFuPry_ufzY7D9ffbp8t28sbHVutjDC6NQoW0CndSt2bYdtr7pWmwPaHWilnQVhWnTGyIMewXQSRKe6wwgAF-z12baM-X1FysPsqTZiAsaVBilA9Vqrrkpf_SO9jWsKpbmiUkL2qlWqqORZZVMkSuiGu_GKaKhbHR5stTDqzFDRhiOm-87_g_4Agyqo9Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1020182522</pqid></control><display><type>article</type><title>Replication-competent gamma-retrovirus Mo-MuLV expressing green fluorescent protein as efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as primary cell receptor</title><source>SpringerNature Journals</source><creator>Stepanov, O. A. ; Prokof’eva, M. M. ; Stocking, K. ; Varlamov, V. P. ; Levov, A. N. ; Vikhoreva, G. A. ; Spirin, P. V. ; Mikhailov, S. N. ; Prassolov, V. S.</creator><creatorcontrib>Stepanov, O. A. ; Prokof’eva, M. M. ; Stocking, K. ; Varlamov, V. P. ; Levov, A. N. ; Vikhoreva, G. A. ; Spirin, P. V. ; Mikhailov, S. N. ; Prassolov, V. S.</creatorcontrib><description>The effect of sulfated polysaccharides on the efficiency of the infection of mouse transplantable fibroblast SC-1 and NIH-3T3 cell lines by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV), which carries the eGFP gene, was investigated. It was found that sulfated polysaccharides have no cytostatic and cytotoxic effects on SC-1 and NIH-3T3 cells in the concentration range of 0.01–100 μg/mL and have virucidal activity against Mo-MuLV. Polysaccharides in the indicated concentrations inhibit Mo-MuLV infection that prevents the further development of viral infection. It was shown that sulfated polysaccharides are also effective inhibitors of other retroviruses, including lentiviruses, which use sulfated polysaccharides as primary cell receptors.</description><identifier>ISSN: 0026-8933</identifier><identifier>EISSN: 1608-3245</identifier><identifier>DOI: 10.1134/S0026893312030119</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Cellular biology ; Cytotoxicity ; Fibroblasts ; Gene expression ; Green fluorescent protein ; Heparan sulfate ; Human Genetics ; Infection ; Lentivirus ; Life Sciences ; Molecular biology ; Molecular Biomedicine Special Issue ; Moloney murine leukemia virus ; Polysaccharides ; Proteins ; Retrovirus ; Viruses</subject><ispartof>Molecular biology (New York), 2012-05, Vol.46 (3), p.457-466</ispartof><rights>Pleiades Publishing, Ltd. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-43d3df2d153ef9950657e582759abec63929fc30a5efaa1b9d3a7130727bd333</citedby><cites>FETCH-LOGICAL-c349t-43d3df2d153ef9950657e582759abec63929fc30a5efaa1b9d3a7130727bd333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0026893312030119$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0026893312030119$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Stepanov, O. A.</creatorcontrib><creatorcontrib>Prokof’eva, M. M.</creatorcontrib><creatorcontrib>Stocking, K.</creatorcontrib><creatorcontrib>Varlamov, V. P.</creatorcontrib><creatorcontrib>Levov, A. N.</creatorcontrib><creatorcontrib>Vikhoreva, G. A.</creatorcontrib><creatorcontrib>Spirin, P. V.</creatorcontrib><creatorcontrib>Mikhailov, S. N.</creatorcontrib><creatorcontrib>Prassolov, V. S.</creatorcontrib><title>Replication-competent gamma-retrovirus Mo-MuLV expressing green fluorescent protein as efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as primary cell receptor</title><title>Molecular biology (New York)</title><addtitle>Mol Biol</addtitle><description>The effect of sulfated polysaccharides on the efficiency of the infection of mouse transplantable fibroblast SC-1 and NIH-3T3 cell lines by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV), which carries the eGFP gene, was investigated. It was found that sulfated polysaccharides have no cytostatic and cytotoxic effects on SC-1 and NIH-3T3 cells in the concentration range of 0.01–100 μg/mL and have virucidal activity against Mo-MuLV. Polysaccharides in the indicated concentrations inhibit Mo-MuLV infection that prevents the further development of viral infection. It was shown that sulfated polysaccharides are also effective inhibitors of other retroviruses, including lentiviruses, which use sulfated polysaccharides as primary cell receptors.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cellular biology</subject><subject>Cytotoxicity</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Green fluorescent protein</subject><subject>Heparan sulfate</subject><subject>Human Genetics</subject><subject>Infection</subject><subject>Lentivirus</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Molecular Biomedicine Special Issue</subject><subject>Moloney murine leukemia virus</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>Retrovirus</subject><subject>Viruses</subject><issn>0026-8933</issn><issn>1608-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcGKFDEQhoMoOK4-gLeAFy-tSWp6unOURVdhFkEXr00mXZnJ0p20qbTo8_liJsyCi4sQSKrq__5UUYy9lOKNlLB9-1UItes1gFQChJT6EdvInegbUNv2MdvUclPrT9kzolshZDlqw35_wWXy1mQfQ2PjvGDGkPnRzLNpEuYUf_i0Er-OzfW6_8bx55KQyIcjPybEwN20xpKxlVpSzOgDN8TROW99TeYYJ-5i4mQrUMnouA8nf_A5JqrR34-QeD6ZzMuLn3AxyQRO6-RMxmq7JD-b9ItbnKZCWVyKxXP2xJmJ8MXdfcFuPry_ufzY7D9ffbp8t28sbHVutjDC6NQoW0CndSt2bYdtr7pWmwPaHWilnQVhWnTGyIMewXQSRKe6wwgAF-z12baM-X1FysPsqTZiAsaVBilA9Vqrrkpf_SO9jWsKpbmiUkL2qlWqqORZZVMkSuiGu_GKaKhbHR5stTDqzFDRhiOm-87_g_4Agyqo9Q</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Stepanov, O. A.</creator><creator>Prokof’eva, M. M.</creator><creator>Stocking, K.</creator><creator>Varlamov, V. P.</creator><creator>Levov, A. N.</creator><creator>Vikhoreva, G. A.</creator><creator>Spirin, P. V.</creator><creator>Mikhailov, S. N.</creator><creator>Prassolov, V. S.</creator><general>SP MAIK Nauka/Interperiodica</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20120501</creationdate><title>Replication-competent gamma-retrovirus Mo-MuLV expressing green fluorescent protein as efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as primary cell receptor</title><author>Stepanov, O. A. ; Prokof’eva, M. M. ; Stocking, K. ; Varlamov, V. P. ; Levov, A. N. ; Vikhoreva, G. A. ; Spirin, P. V. ; Mikhailov, S. N. ; Prassolov, V. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-43d3df2d153ef9950657e582759abec63929fc30a5efaa1b9d3a7130727bd333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cellular biology</topic><topic>Cytotoxicity</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>Green fluorescent protein</topic><topic>Heparan sulfate</topic><topic>Human Genetics</topic><topic>Infection</topic><topic>Lentivirus</topic><topic>Life Sciences</topic><topic>Molecular biology</topic><topic>Molecular Biomedicine Special Issue</topic><topic>Moloney murine leukemia virus</topic><topic>Polysaccharides</topic><topic>Proteins</topic><topic>Retrovirus</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stepanov, O. A.</creatorcontrib><creatorcontrib>Prokof’eva, M. M.</creatorcontrib><creatorcontrib>Stocking, K.</creatorcontrib><creatorcontrib>Varlamov, V. P.</creatorcontrib><creatorcontrib>Levov, A. N.</creatorcontrib><creatorcontrib>Vikhoreva, G. A.</creatorcontrib><creatorcontrib>Spirin, P. V.</creatorcontrib><creatorcontrib>Mikhailov, S. N.</creatorcontrib><creatorcontrib>Prassolov, V. S.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Molecular biology (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stepanov, O. A.</au><au>Prokof’eva, M. M.</au><au>Stocking, K.</au><au>Varlamov, V. P.</au><au>Levov, A. N.</au><au>Vikhoreva, G. A.</au><au>Spirin, P. V.</au><au>Mikhailov, S. N.</au><au>Prassolov, V. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Replication-competent gamma-retrovirus Mo-MuLV expressing green fluorescent protein as efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as primary cell receptor</atitle><jtitle>Molecular biology (New York)</jtitle><stitle>Mol Biol</stitle><date>2012-05-01</date><risdate>2012</risdate><volume>46</volume><issue>3</issue><spage>457</spage><epage>466</epage><pages>457-466</pages><issn>0026-8933</issn><eissn>1608-3245</eissn><abstract>The effect of sulfated polysaccharides on the efficiency of the infection of mouse transplantable fibroblast SC-1 and NIH-3T3 cell lines by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV), which carries the eGFP gene, was investigated. It was found that sulfated polysaccharides have no cytostatic and cytotoxic effects on SC-1 and NIH-3T3 cells in the concentration range of 0.01–100 μg/mL and have virucidal activity against Mo-MuLV. Polysaccharides in the indicated concentrations inhibit Mo-MuLV infection that prevents the further development of viral infection. It was shown that sulfated polysaccharides are also effective inhibitors of other retroviruses, including lentiviruses, which use sulfated polysaccharides as primary cell receptors.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S0026893312030119</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-8933 |
| ispartof | Molecular biology (New York), 2012-05, Vol.46 (3), p.457-466 |
| issn | 0026-8933 1608-3245 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1032899273 |
| source | SpringerNature Journals |
| subjects | Biochemistry Biomedical and Life Sciences Cellular biology Cytotoxicity Fibroblasts Gene expression Green fluorescent protein Heparan sulfate Human Genetics Infection Lentivirus Life Sciences Molecular biology Molecular Biomedicine Special Issue Moloney murine leukemia virus Polysaccharides Proteins Retrovirus Viruses |
| title | Replication-competent gamma-retrovirus Mo-MuLV expressing green fluorescent protein as efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as primary cell receptor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T20%3A31%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Replication-competent%20gamma-retrovirus%20Mo-MuLV%20expressing%20green%20fluorescent%20protein%20as%20efficient%20tool%20for%20screening%20of%20inhibitors%20of%20retroviruses%20that%20use%20heparan%20sulfate%20as%20primary%20cell%20receptor&rft.jtitle=Molecular%20biology%20(New%20York)&rft.au=Stepanov,%20O.%20A.&rft.date=2012-05-01&rft.volume=46&rft.issue=3&rft.spage=457&rft.epage=466&rft.pages=457-466&rft.issn=0026-8933&rft.eissn=1608-3245&rft_id=info:doi/10.1134/S0026893312030119&rft_dat=%3Cproquest_cross%3E1032899273%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1020182522&rft_id=info:pmid/&rfr_iscdi=true |